摘要
目的 :测定阿归养血颗粒中阿魏酸的含量。方法 :用高效液相色谱法 (HPLC)测定阿魏酸的含量。色谱分析采用DiamonsilTM(砖石 )C18色谱分析柱 ,柱温 30℃ ;甲醇 醋酸 (5 0∶5 0 )为流动相 ,紫外检测波长为 313nm ,流速 1 0ml/min ,实验数据用Gilson 715液相色谱工作站处理 ,以峰面积外标法定量。结果 :5种质控样品的平均回收率为 10 0 4 4 % (V/V) ,日内和日间差RSD为 2 0 8%。结论 :本法精度度好 ,方法简便、快速 ,重视性好 。
Objective: To determine the content of ferulic acid in Aguiyangxue granules. Methods: HPLC was used to determine the ferulic acid content, and the chromatographic separation was performed on a Diamonsil TmC 18 column. The column temperature was 30℃ with methanol-1.0% NH 4AC (50∶50) as mobile phase. The detection wavelength was 313 nm, and the flow rate 1 ml·min -1. The test data were treated with Gilson 715 chro-matographic workstation. Peak area calculated represented the amount. Results: The average recovery was 100.44% for the 5 batches of samples. Conclusions: The method is accurate, simple and sensitive. This method has good recovery rate and reliability. It could be used for controlling the content standard of ferulic acid.
出处
《贵阳医学院学报》
CAS
2004年第3期232-233,共2页
Journal of Guiyang Medical College
关键词
色谱法
高压液相
植物制剂
阿魏酸
阿归养血颗粒
chromatography,high pressure liquid
plant preparation
ferulic acid
Aguiyangxue granules
