摘要
以缢蛏Sinonvacula constricta (Lamarck)为研究对象,用双相电泳及图像分析等蛋白质组学技术探求其在高温、低盐2种生态压力下血淋巴内40KDa以下小分子碱性蛋白质的差异表达,以寻找缢蛏血淋巴的抗性机制,明确血淋巴在机体防御体系中的作用。 在环境压力下,缢蛏血淋巴内有多种蛋白发生变化,MALDI-TOF-MS检测鉴定出磷脂酶A2、表皮分泌蛋白xp2前体、脑衍生神经营养因子、肿瘤坏死因子受体、脯氨酸顺反同工酶等7种蛋白或多肽。涉及多种与炎症反应、基质转运、神经营养、基因表达调控、蛋白质合成等有关的功能。说明其血淋巴在缢蛏的防御体系中具有关键性作用。
Sinonovacula constricta(Lamarck), a kind of mollusks, is an important species of marine aquaculture. We choose it as our experiment sample and its basic protein expression profiles of hemolymph after short-term treatment ur(?)der high temperature and low salinity were detected, seven kinds of proteins or polypeptides were identified: Cysteine-rich protein 2 CRP2, Phospholipase A2, Pepidyl-prolyl cis- trans isomerase A, epidermal secretory protein xp2 precursor, brain-derived neurotrophic factor, tumor necrosis factor receptor, highmobility groupprotein HMGI-C. They have multiple functions, such as nutrition, gene expression regulation, protein synthesis, substances transport, antitoxin and anti-interference. The results suggested that the hemolymph played a key action in defense System of Sinonovacula constricta .
出处
《中国海洋药物》
CAS
CSCD
2004年第3期23-27,共5页
Chinese Journal of Marine Drugs
基金
福建省教育厅资助项目(A类)(项目编号:JA02179)
关键词
双相电泳
图像分析
缢蛏
血淋巴碱性蛋白
高温低盐
biphase electrophoresis
image analysis
Sinonovacula constricta (Lamarck)
basic protein in hemolymph
high temperature and low salinity
