摘要
从发病鸡的粪便中分离出具有多重耐药性的大肠杆菌O18和鸡白痢沙门菌O2,检测多黏菌素E的最小抑菌浓度(MIC)和抗生素后效应(PAE),测定多黏菌素E接触过的细菌各组乳酸脱氢酶(LDH)和γ-谷氨酰基转移酶(γ-GT)活性变化、电镜观察细胞膜形态、巨噬细胞吞噬细菌数量和细菌对Caco-2的黏附力。多黏菌素E作用后,菌液中LDH和γ-GT活性显著性增高(P<0.01),细菌胞膜出现破损或缺失,细菌形态改变,被巨噬细胞吞噬的菌量明显增多(P<0.01),细菌对Caco-2的黏附力显著下降(P<0.01)。多黏菌素E对鸡源性多重耐药菌株存在PAE,推测其产生机制为破坏细菌细胞膜,改变细菌形态,促进巨噬细胞对细菌的吞噬能力增强,降低细菌对细胞黏附力。
E.coli O18 and S.pullorum O2 were isolated from excrements of infected chickens.The minimum inhibitory concentration(MIC)and post antibiotic effect(PAE)of colistin were detected and the change of lactic dehydrogenase(LDH)and gamma-glutamyltransferase(γ-GT),membrane morphology,the number of macrophages engulfing bacteria and bacteria adhesion were investigated.Contrasted with control group,activity of LDH andγ-GT significantly increased in bacterium solution(P<0.01),bacterial cell membranes damaged,bacteria morphology changed,the number of bacteria engulfed by macrophages significantly increased(P<0.01),and the number of bacteria adhered to Caco-2cells significantly decreased(P<0.01)in colistin groups.Colistin has PAE on chicken-derived E.coli O18 and S.pullorum O2.The mechanism may be related with colistin destroying bacterial cell membranes,intensifying phagocytosis of macrophages and decreasing the number of bacteria adhesion.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2015年第4期585-591,共7页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(31472240)
黑龙江省自然科学基金资助项目(C201424)
