摘要
目的拆分西酞普兰(R,S-citalopram)对映异构体,建立S-西酞普兰的质量检测方法.方法采用CHIROBIOTIC V手性柱,以甲醇-冰醋酸一三乙胺(100:0.1:0.1)为流动相,检测波长240nm,柱温20℃,流速1.0min/ml.结果 S、R型异构体获得完全分离,S-西酞普兰在10～150μg/m1范围内具有良好线性,(r=0.999 1,n=5).结论该方法简便、准确,可作为S-西酞普兰含量测定及其R型异构体的含量监控方法.
Objective To separate the enantiomers of citalopram and determine the content of S-citalopram using chiral high-performance liquid chromatography (HPLC). Methods The chiral column CHIROBIOTIC V was used with the mobile phase using methanol-acetic acid-triethylamine (100∶0.1∶0.1) at the detection wavelength of 240 nm, column temperature of 20 ℃and flow rate of 1.0 ml/min. Results Complete separation of the enantiomers of citalopram was achieved, and S-citalopram exhibited good linearity within the concentration range of 10 to 150 μg/ml (r=0.9991, n=5). Conclusion This method allows accurate quantification of S- and R-citalopram and is well suited for drug interaction investigations.
出处
《第一军医大学学报》
CSCD
北大核心
2004年第6期716-717,共2页
Journal of First Military Medical University
