摘要
确立了蒙古黄芪中黄芪甲苷含量的高效液相色谱测定方法(HPLC),色谱柱:C18(150×4.6mm,5μm)流动相为乙腈:水:磷酸=1:2:0.1,流速为1.20mL/min,检测器:紫外检测波长200nm。
To establish a HPLC method for determination of Astragaloside IV Astragalus membranaceus ( Fisch ) Bge.var. mongholicus ( Bge ) Hsiao. The G18 column ( 150×4.6mm, 5μm) was used with a mobile of acetonitrile - water - phosphoric acid (1:2:0.1).The flow rate was 1.20mL/min. The detection wavelength was 200nm.Conclusion that the method is suitable for assay of Astragaloside IV in Astragalus membranaceus (Fisch ) Bge.var. mongholicus ( Bge ) Hsiao.
出处
《齐齐哈尔大学学报（自然科学版）》
2004年第1期20-22,共3页
Journal of Qiqihar University(Natural Science Edition)
关键词
高效液相色谱法
测定
蒙古黄芪
黄芪甲苷
含量测定
豆科
HPLC
Astragalus membranaceus ( Fisch ) Bge.var. mongholicus ( Bge ) Hsiao
Astragaloside IV
