摘要
利用DMSO促进杂交瘤细胞分泌单克隆抗体 ,并对其作用机制进行了研究。结果表明在杂交瘤细胞对数生长后期添加 0 6 %浓度的DMSO与对照相比可提高抗体的产量 2倍 ;流式细胞仪检测细胞周期表明此时 83 7%的细胞处于G1期 ;免疫印记结果显示在有DMSO作用下杂交瘤细胞P2 7蛋白表达显著升高。因此 ,DMSO可通过促进P2 7蛋白的表达 ,抑制杂交瘤细胞的增殖 ,从而提高杂交瘤细胞分泌抗体的能力。
Dimethyl sulfoxide (DMSO), a well known differentiation inducer in several myeloid cells, induces G1 phase arrest in many cell lines. In this study, we investigated the possibility of using DMSO to arrest H18 hybridoma cells to the G1 phase and monitor whether the arrest improves antibody production. We showed that DMSO in concentration ranging between 0 3% and 0 6% efficiently arrested H18 hybridoma cells in G1 phase. In our experiment, >80% of cells grown for 36h in presence of the 0 6% DMSO were arrested in G1 Furthermore, expression levels of P27 were up regulated tow fold during the G1 phase. Higher concentration of DMSO at 0 9% leads to cytotoxicity. Herein we show a simple way, a two stage process for antibody production, which consists of a proliferation phase leading to the desired cell density, followed by an extended production phase during which the cells remain at G1 phase. Our observation that the addition of DMSO results in increase antibody production is of significance in further use of hybridoma cells in high density large scale cell culture.
出处
《生物工程学报》
CAS
CSCD
北大核心
2004年第4期568-571,共4页
Chinese Journal of Biotechnology
基金
国家 8 63计划生物工程主题项目 (No .2 0 0 2AA2 170 11)
国家重大科技专项 (No.2 0 0 2AA2Z3 441)~~
