摘要
目的：建立一种安息香荧光衍生法测定面粉及其制品中硼砂根的含量。方法安息香与硼离子形成复合物，在甘氨酸做缓冲溶液条件下，通过荧光分光光度法测定复合物中的硼，进而建立一种快速检测食品中的硼砂含量的方法。结果在该条件下硼砂根为10-7~10-5 mol/L范围内回归系数达到0.9999。利用该条件建立的测定面制品中硼砂的含量回收率为83%~102%，其中面粉中的回收率在100%左右。结论本研究证明使用该方法测定面粉及其制品中的硼酸根较为准确、可靠，为方便快速地测定食品中的硼砂提供了技术支持。
Objectvie To develop a benzoin fluorescence method for the determination of borax in flour and its products. Methods Benzoin and boron ions formed complexes in glycine buffer solution and then de-termined by fluorescence spectrophotometry, thereby establishing a method for rapid detection of food borax content. Results Under the described conditions, the proposed method was applicable over the concentration range ( 10-7~10-5 mol/L), the borate concentration was linear with the fluorescence intensity (regression coef-ficient: 0.9999), with recoveries for borax in flour and related products were almost 100and 83~102 respec-tively. Conclusion The established method for determination of borax in flour and its products is accurate and validated, which provides efficient technical support for rapidly detecting the borax in food.
出处
《食品安全质量检测学报》
CAS
2014年第3期929-933,共5页
Journal of Food Safety and Quality
基金
河北省科技支撑项目(13227105D)~~
关键词
荧光
硼砂
面粉及其制品
fluorescence
borax
flour and its products
