摘要
枯草杆菌是重要的工业生产菌。由于它的非病原性及可将产生的多种酶分泌至胞外的特点,也已成为基因工程重要的宿主菌之一.本文叙述了枯草杆菌中的多种酶基因的克隆及表达。对枯草杆菌作为基因工程的宿主菌应具备的条件、克隆载体的构建和外源基因在枯草杆菌中的表达也做了简介。
Bacillus subtilis is an important industrial enzyme—producing bacterium.It has became a potential host cell for genetic engineering because of its nonpathogenicity and the ability of secreting enzyme into medium.This review describes the physiological characteristics of B. subtilis,the produced enzyme and the cloning and expression of certain enzyme genes from B.subtilis.For its application in genetic engineering,the reguirements for B.subtilis,the construction of specific cloning vectors and the heterogenes expressed in it are presented as well。
关键词
枯草杆菌
基因克隆
分子生物学
bacillus subtilis
gene cloning
cloning vectoy
