摘要
目的观察卵巢切除小鼠的骨折早期愈合情况,并探讨其可能的机制。方法 40只6周龄雌性C57BL/6小鼠随机分为卵巢切除组(OVX组)和假手术组,每组20只。于OVX组小鼠卵巢切除术后4周,两组分别建立右侧股骨骨折模型。建模后2周,X线摄片观察骨折愈合情况;M icro-CT测定骨痂体积和骨密度,观察血管灌注后骨折部位血管化情况并测定血管体积和血管体积分数;制作骨组织标本,HE染色光学显微镜观察骨折愈合进程,免疫组织化学染色观察骨痂组织血管内皮生长因子(VEGF)蛋白表达。结果 X线片观察和骨折标本组织学检查显示:OVX组小鼠早期骨折愈合明显较假手术组延迟。M icro-CT检测结果显示:与假手术组比较,OVX组骨痂体积较小,骨密度较低,血管灌注后骨折部位新生血管少且血管体积和血管体积分数较小,且差异均有统计学意义(P<0.05)。免疫组织化学染色观察发现OVX组骨痂组织中VEGF表达明显少于假手术组。结论卵巢切除后股骨骨折小鼠的骨折早期愈合明显延迟,可能与骨痂组织新生血管减少有关。
Objective To observe the early fracture healing in ovariectomized mice,and explore the possible mechanism.Methods Forty female C57BL/6 mice aged 6 weeks were randomly divided into ovariectomy group(OVX group) and sham operation group,with 20 rats in each group.Four weeks after ovariectomy in OVX group,right femur fracture mouse models were established in both groups.Two weeks after model establishment,the fracture healing was observed by X-ray examinations,and Micro-CT was used to measure the volumes of callus and mineral density,observe the angiogenesis and determine the vessel volumes and vessel volume fractions in fracture sites after vessel perfusion.Bone tissue samples were prepared 2 weeks after model establishment,the fracture healing was observed by light microscopy with HE staining,and the expression of vascular endothelial growth factor(VEGF) protein in callus tissues was observed with immunohistochemical staining. Results X-ray examinations and histological examinations of fracture samples indicated that the early fracture healing in OVX group was significantly delayed compared with sham operation group.Micro-CT detection revealed that compared with sham operation group,the volume of callus,mineral density,and number of vessels,vessel volume and vessel volume fraction in fracture sites after vessel perfusion were significantly decreased(P<0.05).It was observed by immunohistochemical staining that the expression of VEGF in callus tissues in OVX group was significantly lower than that in sham operation group. Conclusion The early fracture healing is significantly delayed in ovariectomized mice,which may be related to the reduction of angiogenesis in callus tissues.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2011年第7期890-894,共5页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家自然科学基金(30872641)
上海市研发基地项目(09dz2200500)~~
关键词
骨质疏松
卵巢切除
骨折愈合
osteoporosis
ovariectomy
fracture healing
