摘要
目的 :探讨端粒酶活性、血管内皮生长因子 (VEGF)在非小细胞肺癌 (NSCLC)组织中的表达及其临床意义。方法 :对 70例NSCLC肺癌组织采用端粒末端重复序列扩增 -酶联免疫试验法检测端粒酶活性和免疫组化法检测VEGF的表达 ,比较二者的表达与生存率的关系。结果 :端粒酶和VEGF在NSCLC中的阳性表达率分别为 74 .3%和 70 .0 % ;二者呈正相关 (P =0 .0 32 ,r=0 .2 5 7) ;端粒酶、VEGF表达阳性者生存时间明显低于阴性表达者 (P <0 .0 5 )。结论 :端粒酶与VEGF在NSCLC组织中的表达呈正相关 ;二者联合检测对患者化疗疗效。
Objective: To evaluate the expression and clinical significance of telomerase activity and VEGF in non-small-cell lung cancer (NSCLC). Method:The telomerase activity was detected by telomeric repeat amplification protocol-ELISA and VEGF was detected by the method of immunohistochemical techniques in 70 cases of NSCLC, comparing the relationship between the expression of telomerase activity 、VEGF and the survival rate. Result: The positive expression rate of telomerase activity and VEGF was 74.3% and 70.0%. Telomerase activity and VEGF have positive correlation in the expression of lung cancer tissues (p=0.032,r=0.257);And the survival time of NSCLC person who was the positive expression of telomerase activity and VEGF was shorter than the negative expression. Conclusion: Telomerase activity and VEGF had positive correlation in the expression of NSCLC,the combine detection of telomerase activity and VEGF had the scientific instructional action.
出处
《河北医学》
CAS
2004年第12期1071-1073,共3页
Hebei Medicine
