摘要
目的观察从艾氏腹水瘤小鼠瘤细胞中提取的热休克蛋白70-抗原肽复合物(HAC70)诱导的抗肿瘤免疫效应,探讨其临床应用。方法提取热休克后的小鼠艾氏腹水瘤细胞经肝素-琼脂糖亲和层析纯化,制成热休克蛋白-肽复合物疫苗,以体内免疫法检测HAC70的抑瘤作用,3H-TdR掺入释放法检测脾细胞对肿瘤细胞的杀伤活性。结果经HAC70免疫的荷瘤小鼠皮下肿瘤结节平均重1.26±0.4 g,较对照组(3.14±0.56 g)明显减轻(P<0.05);当效靶比为200:1时,免疫组脾细胞杀伤活性为49.25±11.25%,显著高于对照组(18.50±9.95%,P<0.01)及荷瘤组(25.75±8.38%,P<0.01)。结论经肝素-琼脂糖亲和层析纯化的HAC70可通过诱导机体产生特异性杀伤艾氏腹水瘤细胞的活性,明显抑制荷瘤小鼠艾氏腹水瘤的生长。
Objective To investigate the anti-tumor immunoefficacy induced by HAC70 derived from Ehrlich ascites tumor cell and to study its clinical application.Methods After the heat-shock HSP70 from the Ehrlich ascites tumor cell was isolated and pureed by Heparin-Sepharose chromatography and got the HAC70 vaccine. The anti-tumor efficacy of splenocyte was determined by the3H-TdR release assay.Results The average tumor weight of the mice received HAC70 immunization was only 1.26±0.4 g,obvious difference(P<0.05)observed when contrasted with the control group(3.14±0.56 g).It was showed that specific cytotoxitic rate of splenocytes from the immunized mice to the Ehrlich ascites tumor cells was 49.75±8.93%,significantly higher than from the tumor-bearing(18.5±9.95%)and control group(25.75±8.38,P<0.01).Conclusion HAC70 pureed by Heparin-Sepharose chromatography have the activities of inflicting the Ehrlich ascites tumor cell and inhibit obviously growth of the Ehrlich ascites tumor.
出处
《中国实验诊断学》
2007年第11期1441-1444,共4页
Chinese Journal of Laboratory Diagnosis
