摘要
This study was conducted to evaluate the effects of ageing corn levels(stored for 4 years)with or without the supplementation of tea polyphenols(TPP)on the performance,egg quality and antioxidant status of laying hens.A total of 288 Lohmann commercial laying hens(63-week-old)were used under a 2×4 factorial arrangement with 4 levels of dietary ageing corn(0%,25%,50%,or 100%)and 2 levels of TPP(0 and 600 mg/kg)for 8 wk.Dietary ageing corn linearly decreased(P<0.05)the egg production,serum total antioxidant capacity(T-AOC),liver glutathione peroxidase(GSH-Px)of laying hens,yolk index,yolk colour,1,1-diphenyl-2-picrylhydrazyl(DPPH)value and the reducing power value of egg yolk,but it linearly increased(P<0.05)the feed conversion rate,ovary malondialdehyde(MDA)content of laying hens,and the protein carbonyl content of egg yolk.Tea polyphenol supplementation increased(P<0.05)the serum T-AOC,serum superoxide dismutase(SOD),liver SOD,liver GSH-Px,ovary SOD,GSH-Px,the expression of antioxidant-related genes of laying hens,albumen height,Haugh unit,DPPH value and the majority free amino acids of egg yolk,but it decreased(P<0.05)the serum MDA content of laying hens,MDA and protein carbonyl of egg yolk.In conclusion,the ageing corn significantly reduced the perfor-mance,egg quality,antioxidant status and egg antioxidant capacity of laying hens,while TPP supple-mentation partially counteracted the adverse effects,especially antioxidant status and egg antioxidant capacity of laying hens.
基金
financially supported by the National Science and Technology Project(2014BAD13B04)
Sichuan Provincial Science and Technology Project(2018NZ20009)
