摘要
建立了微乳中利福昔明含量的高效液相色谱检测方法(HPLC)。采用C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-乙腈-0.075 mol/L磷酸二氢钾溶液-1 mol/L柠檬酸溶液(32∶28∶25∶4)为流动相,流速1.0 mL/min,检测波长240 nm,进样量10μL,柱温25℃。利福昔明浓度在4.79~95.7μg/mL时,其峰面积与浓度线性关系良好(R^(2)=0.9997),稳定性好,RSD均小于2%,回收率高,平均回收率为98.98%。该法操作简便,分析快速,结果准确,适用于利福昔明微乳剂的含量测定。
A method for the content determination of rifaximin microemulsion by HPLC was established.HPLC system consisted of C18 column(4.6 mm×250 mm,5μm),methyl alcohol-acetonitrile-citric acid-potassium phosphate monobasic(32∶28∶25∶4)as mobile phase.The flow rate was 1.0 mL/min.The detection wave length was 240 nm.The injection volume was 10μL and column temperature was room temperature.The calibration curve was linear in the range of 4.79~95.7μg/mL(R^(2)=0.9997).The average recovery was98.98%.The method is rapid,simple and accurate for the content determination of rifaximin microemulsion.
作者
朱庆贺
苗艳
刘雪松
陈曦
杨旭东
王爽
王观悦
张艳
史同瑞
ZHU Qing-he;MIAO Yan;LIU Xue-song;CHEN Xi;YANG Xu-dong;WANG Shuang;WANG Guan-yue;ZHANG Yan;SHI Tong-rui(Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences,Qiqihar 161005,Heilongjiang,China)
出处
《湖北农业科学》
2020年第S01期438-440,451,共4页
Hubei Agricultural Sciences
基金
黑龙江科研机构创新能力提升专项(YC2016D004)
