摘要
以艾氏腹水癌细胞接种到小鼠右后肢掌内侧皮内,纪起腘窝淋巴结转移为模型,观察瘤细胞转移到淋巴结后的命运及淋巴结本身的变化。实验共四十天,在不同时间取其引流淋巴结往光学显微镜下观察。发现接种1小时后,瘤细胞可沿淋巴管移至腘窝淋巴结;5小时后瘤细胞有核分裂相,并可达第二站引流淋巴结,即髂动脉旁淋巴结;24小时后有明显的转移灶形成。瘤细胞进入淋巴结后由边缘窦到达中间窦,后达髓窦。三天后淋巴结出现明显的反应性增生,如毛细血管后小静脉周围出现许多运动型淋巴细胞、窦组织细胞增生和生发中心增大等。同时瘤细胞也开始出现变性及坏死的改变。电子显微镜下观察,发现淋巴结内的瘤细胞周围有许多淋巴细胞与之紧贴在一起,同时这些淋巴细胞胞浆中的线粒体及高尔基器均向细胞方向集结,并有向瘤细胞释放某种物质的现象。实验结果提示:加强和提高淋巴结防御肿瘤转移的能力,可能是抵抗肿瘤转移的重要途径之一。
The purpose of this experiment is to study the destiny of cancer cells and changes of lymph node after lymphatic metastasis.Ehrlich ascitic carcinoma cells were injected intradermally into the right footpad of mice.We try to estabilish a model about lymphatic metastasis in the popliteal lymph node.The lymph nodes of the drainage area were taken out at different time intervals and examined by light microscopy,transmission electron microscopy as well as scanning electron microscopy.Onehour after the injection cancer cells were found in the popliteal lymph node.Mitotic figures of cancer cells were discovered in fifth hours and the tumor cells had reached to second lymph node——lumber lymph node or parailiac lymph node.The metastasis were formed in 24 hours after injection.The tumor cells first migrated in the subcapsular sinus of lymph node,then they entered the intermediate and medullary sinuses.Besides,the lymph nodes showed active reactionary proliferation,hyperplasia of histocytes and enlargement of germinal centers after three days.There were many small lymphocytes assuming the form of active amebae,surrounding the postcapillary venules.Degeneration and necrosis of cancer cells was observed at same time.Under the electron microscope many lymphocytes were observed to adhere closely to the cancer cell and their Golgi apparatus and mitochondria were accumulated towards the cell memberane opposing the cancer cell,as if some substance were released to it.This experimental result showed that lymph node probably prosess ability to defend metastasis of cancer cells.
作者
孙异临
高进(指导)
杨简(指导)
李宝贵
Sun Yilin;Gao Jin;Yang Jian;Li Baogui(Beijing Neurosurgical Institute;Laboratory of Experimental Oncology,Department of Pathology Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences)
出处
《解剖学报》
CAS
1982年第1期97-102,122-123,共8页
Acta Anatomica Sinica
