摘要
目的:分析维生素E结合蛋白(Afamin)、邻苯二甲酸二丁酯(DBP)在原发性卵巢功能不全(POI)患者血清中的表达情况,及两者与卵巢功能的相关性。方法:回顾性选取2016年12月至2019年12月在我院就诊的150例POI患者作为POI组,同时选取同时间段的150例健康者为对照组进行研究;酶联免疫吸附法(ELISA)检测所有受试者血清中Afamin表达水平及卵巢功能性指标,采用气相色谱-质谱联用仪对血清中DBP的含量进行测定,Pearson相关分析法分析Afamin、DBP表达水平与促卵泡生长激素(FSH)、雌激素(E2)、促黄体生成素(LH)、抗苗勒管激素(AMH)、黄体酮(P)的相关性,多因素Logistic逐步回归分析法分析影响POI的因素。结果:与对照组相比,POI组血清中Afamin、DBP的表达水平均明显增加(P<0.05);POI患者和对照组血清Afamin、DBP表达水平与FSH、E2呈正相关(P<0.05),与AMH呈负相关(P<0.05);经过多因素Logistic逐步回归分析,结果显示Afamin、DBP为影响POI的独立危险因素(P<0.05)。结论:血清Afamin、DBP水平在POI中表达上调,为影响POI的独立危险因素,Afamin与DBP表达水平差异有望为POI的临床治疗提供参考。
Objective:To analyze the expressions of Afamin and dibutyl phthalate(DBP)in serum of patients with primary ovarian insufficiency(POI)and their correlation with ovarian function.Methods:150 cases of POI in our hospital from December 2016 to December 2019 were retrospectively selected as POI group,and 150 healthy people in the same period were selected as control group;the expression levels of serum Afamin and ovarian function were detected by enzyme-linked immunosorbent assay(ELISA),the level of DBP in serum was determined by gas chromatography-mass spectroscopy,Pearson correlation analysis was used to analyze the correlation of Afamin and DBP expression levels with follicle-stimulating hormone(FSH),estrogen(E2),luteinizing hormone(LH),anti-Müllerian hormone(AMH)and progesterone(P),Multivariate logistic stepwise regression analysis was used to analyze the factors influencing POI.Results:Compared with the control group,the expression levels of Afamin and DBP in serum of the POI group were significantly increased(P<0.05);the expression levels of serum Afamin and DBP were positively correlated with FSH and E2(P<0.05),but negatively correlated with AMH(P<0.05);Multivariate logistic stepwise regression analysis showed that Afamin and DBP were risk factors of POI(P<0.05).Conclusion:The expression levels of serum Afamin and DBP is up-regulated in POI,which are independent risk factors of POI.The differences of Afamin and DBP expression levels may provide reference for the clinical treatment of POI.
作者
颜红丽
单莉
何菊仙
安沛兴
YAN Hongli;SHAN Li;HE Juxian;An Peixing(Department of Obstetrics and Gynecolog,Northwest Women and Children’s Hospital(Shaanxi Maternal and Child Health Hospital),Xi’an 710061,China;Third Department of Gynaecology,Northwest Women and Children’s Hospital(Shaanxi Maternal and Child Health Hospital),Xi’an 710061,China;Department of Family Planning,Northwest Women and Children’s Hospital(Shaanxi Maternal and Child Health Hospital),Xi’an 710061,China)
出处
《现代医学》
2021年第7期788-792,共5页
Modern Medical Journal
