【背景】酪蛋白水解蛋白酶L(caseinolytic protease L,ClpL)是热休克蛋白100(heat shock protein 100,HSP100)家族成员,参与多种细胞内生物学过程包括应激耐受反应、长期存活、毒力和抗生素抗性等,在病原菌的致病过程中发挥重要作用。...【背景】酪蛋白水解蛋白酶L(caseinolytic protease L,ClpL)是热休克蛋白100(heat shock protein 100,HSP100)家族成员,参与多种细胞内生物学过程包括应激耐受反应、长期存活、毒力和抗生素抗性等,在病原菌的致病过程中发挥重要作用。【目的】阐明HSP100/ClpL对猪链球菌2型(Streptococcus suis type 2)强毒株的应激耐受和毒力的影响。【方法】利用温敏自杀质粒pSET4s构建了SsClpL基因缺失株ΔclpL;利用大肠杆菌-猪链球菌穿梭质粒pSET2构建了互补株CΔclpL;比较亲本株SS2-1、ΔclpL和CΔclpL在各种应激环境(高温、氧化性应激和酸应激)和血液中的生存能力;利用小鼠感染模型和体内竞争试验评价clpL缺失前后细菌的毒力变化。【结果】clpL缺失后,SS2在各种应激环境中的生存能力明显减弱;同时在猪全血中的存活能力也显著降低;对BALB/c小鼠的致病力有所降低,在体内的定殖能力明显降低。【结论】SsClpL与猪链球菌的应激耐受和致病性密切相关。展开更多
[Background]Hygrophorus,belonging to Basidiomycota,Agaricomycetes,Agaricales,Hygrophoraceae,is widely distributed all around the world.[Objective]To investigate the species of Hygrophorus and enrich the knowledge abou...[Background]Hygrophorus,belonging to Basidiomycota,Agaricomycetes,Agaricales,Hygrophoraceae,is widely distributed all around the world.[Objective]To investigate the species of Hygrophorus and enrich the knowledge about the species diversity of this genus in Shanxi Province.[Methods]We collected fungal specimens,observed and characterized their morphology.After DNA extraction,the internal transcribed spacer(ITS)was sequenced.Phylogenetic trees were constructed based on the maximum likelihood method and Bayesian inference.[Results]Eleven species of Hygrophorus were identified based on the morphological and molecular evidence,including ten known species and a new species.This article described the macroscopic and microscopic morphological characteristics of the new species Hygrophorus brunneodiscoides in detail.The basidiocarp photos of ten known species and a key to the species of Hygrophorus in Shanxi Province were provided.[Conclusion]We identified a new species,H.brunneodiscoides,in China,confirmed 11 species of Hygrophorus in Shanxi Province,and briefed their geographical distribution and associations with hosts according to the field observation.展开更多
【背景】胞外聚合物是微生物分泌的高分子聚合物,在环境治理、材料科学、生物医学等领域具有重要的应用价值。【目的】探讨胞外聚合物研究现状、研究热点和发展趋势。【方法】采用文献计量学方法,基于Web of Science核心合集数据库,检索...【背景】胞外聚合物是微生物分泌的高分子聚合物,在环境治理、材料科学、生物医学等领域具有重要的应用价值。【目的】探讨胞外聚合物研究现状、研究热点和发展趋势。【方法】采用文献计量学方法,基于Web of Science核心合集数据库,检索近10年胞外聚合物相关文献,利用VOSviewer软件进行统计分析。【结果】胞外聚合物研究发文量呈上升趋势,中国发文量居首,但质量有待提高;研究热点主要集中在环境治理、生物制造和生物矿化等领域;微塑料降解是当前研究热点之一。【结论】本研究为深入了解胞外聚合物研究现状以及把握发展趋势提供了依据。展开更多
文摘【背景】酪蛋白水解蛋白酶L(caseinolytic protease L,ClpL)是热休克蛋白100(heat shock protein 100,HSP100)家族成员,参与多种细胞内生物学过程包括应激耐受反应、长期存活、毒力和抗生素抗性等,在病原菌的致病过程中发挥重要作用。【目的】阐明HSP100/ClpL对猪链球菌2型(Streptococcus suis type 2)强毒株的应激耐受和毒力的影响。【方法】利用温敏自杀质粒pSET4s构建了SsClpL基因缺失株ΔclpL;利用大肠杆菌-猪链球菌穿梭质粒pSET2构建了互补株CΔclpL;比较亲本株SS2-1、ΔclpL和CΔclpL在各种应激环境(高温、氧化性应激和酸应激)和血液中的生存能力;利用小鼠感染模型和体内竞争试验评价clpL缺失前后细菌的毒力变化。【结果】clpL缺失后,SS2在各种应激环境中的生存能力明显减弱;同时在猪全血中的存活能力也显著降低;对BALB/c小鼠的致病力有所降低,在体内的定殖能力明显降低。【结论】SsClpL与猪链球菌的应激耐受和致病性密切相关。
文摘[Background]Hygrophorus,belonging to Basidiomycota,Agaricomycetes,Agaricales,Hygrophoraceae,is widely distributed all around the world.[Objective]To investigate the species of Hygrophorus and enrich the knowledge about the species diversity of this genus in Shanxi Province.[Methods]We collected fungal specimens,observed and characterized their morphology.After DNA extraction,the internal transcribed spacer(ITS)was sequenced.Phylogenetic trees were constructed based on the maximum likelihood method and Bayesian inference.[Results]Eleven species of Hygrophorus were identified based on the morphological and molecular evidence,including ten known species and a new species.This article described the macroscopic and microscopic morphological characteristics of the new species Hygrophorus brunneodiscoides in detail.The basidiocarp photos of ten known species and a key to the species of Hygrophorus in Shanxi Province were provided.[Conclusion]We identified a new species,H.brunneodiscoides,in China,confirmed 11 species of Hygrophorus in Shanxi Province,and briefed their geographical distribution and associations with hosts according to the field observation.
文摘【背景】胞外聚合物是微生物分泌的高分子聚合物,在环境治理、材料科学、生物医学等领域具有重要的应用价值。【目的】探讨胞外聚合物研究现状、研究热点和发展趋势。【方法】采用文献计量学方法,基于Web of Science核心合集数据库,检索近10年胞外聚合物相关文献,利用VOSviewer软件进行统计分析。【结果】胞外聚合物研究发文量呈上升趋势,中国发文量居首,但质量有待提高;研究热点主要集中在环境治理、生物制造和生物矿化等领域;微塑料降解是当前研究热点之一。【结论】本研究为深入了解胞外聚合物研究现状以及把握发展趋势提供了依据。
