Stable isotope analysis was used to determine the relative dietary importance of kelp-derived detritus to plankton and benthic organisms along a gradient of kelp abundance driven by recovering sea otter populations al...Stable isotope analysis was used to determine the relative dietary importance of kelp-derived detritus to plankton and benthic organisms along a gradient of kelp abundance driven by recovering sea otter populations along the west coast of Vancouver Island (WCVI), Canada. The study used region-specific kelp isotope values (δ<sup>13</sup>C and δ<sup>15</sup>N) and season-specific phytoplankton isotope values to model dietary contributions of kelp-derived detritus (KDD). In general, KDD contributions were moderate to high in most plankton size fractions during the summer and decreased during the winter, particularly in the kelp sparse region. Hypothesized regional and spatial (distance from the coast) differences in kelp detritus contributions to zooplankton w<span style="white-space:normal;"><span style="font-family:;" "="">ere<span style="white-space:normal;"><span style="font-family:;" "=""> not evident. Modeled estimates of the KDD contribution to benthic invertebrates w<span style="white-space:normal;"><span style="font-family:;" "="">ere<span style="white-space:normal;"><span style="font-family:;" "=""> high (>40%) and independent of the organism size, among regions and between seasons, with the exception of Astraea gibberosa in the kelp abundant region. Local oceanography, natural kelp isotope signature variation, and significant overlap between kelps’ and blooming phytoplankton isotope values led to a large uncertainty in the assessed KDD contributions in benthic organisms. These results highlighted the importance of the KDD as a widespread and stable year-round food source in coastal kelp populated regions.展开更多
Background: The distribution of forest vegetation within urban environments is critically important as it influences urban environmental conditions and the energy exchange through the absorption of solar radiation and...Background: The distribution of forest vegetation within urban environments is critically important as it influences urban environmental conditions and the energy exchange through the absorption of solar radiation and modulation of evapotranspiration. It also plays an important role filtering urban water systems and reducing storm water runoff.Methods: We investigate the capacity of ALS data to individually detect, map and characterize large(taller than15 m) trees within the City of Vancouver. Large trees are critical for the function and character of Vancouver’s urban forest. We used an object-based approach for individual tree detection and segmentation to determine tree locations(position of the stem), to delineate the shape of the crowns and to categorize the latter either as coniferous or deciduous.Results: Results indicate a detection rate of 76.6% for trees > 15 m with a positioning error of 2.11 m(stem location). Extracted tree heights possessed a RMSE of 2.60 m and a bias of-1.87 m, whereas crown diameter was derived with a RMSE of 3.85 m and a bias of-2.06 m. Missed trees are principally a result of undetected treetops occurring in dense, overlapping canopies with more accurate detection and delineation of trees in open areas.Conclusion: By identifying key structural trees across Vancouver’s urban forests, we can better understand their role in providing ecosystem goods and services for city residents.展开更多
The province of British Columbia, Canada, has established an Agricultural Land Reserve (ALR) to protect the most suitable soil landscapes for agriculture. Increases in population and urbanization have resulted in deve...The province of British Columbia, Canada, has established an Agricultural Land Reserve (ALR) to protect the most suitable soil landscapes for agriculture. Increases in population and urbanization have resulted in development challenges on ALR lands. The Metro Vancouver area is the most productive agricultural area in British Columbia as well as the most rapidly growing urban region. The increase in impervious areas has decreased the amount of arable land for soil-based agriculture and altered the hydrological cycle. Analysis using a combination of aerial imagery and GIS found that impervious areas comprise about 10 percent of the ALR within Metro Vancouver. Farm residences and greenhouses have the largest effect on reducing the soil surface for water infiltration. This decrease in area has negatively influenced the ecosystem heath of the region, as well as, decreasing the amount of agricultural land for soil based agriculture and both surface and groundwater dynamics.展开更多
Objectives: Immigrants of Chinese ethnicity and young people (between 18 and 30 years of age) are known to access health services less frequently and may be at greater risk for experiencing unmet health needs. The pur...Objectives: Immigrants of Chinese ethnicity and young people (between 18 and 30 years of age) are known to access health services less frequently and may be at greater risk for experiencing unmet health needs. The purpose of this study was to examine the health beliefs, health behaviors, primary care access, and perceived unmet healthcare needs of Chinese young adults.Methods: Semi-structured in-depth interviews were carried out with eight Chinese young adults in Vancouver, Canada. Results: A content analysis revealed that these Chinese young adults experienced unmet healthcare needs, did not have a primary care provider, and did not access preventive services. Cultural factors such as strong family ties, filial piety, and the practice of Traditional Chinese Medicine influenced their health behaviors and healthcare access patterns. Conclusion: Chinese young adults share similar issues with other young adults in relation to not having a primary care provider and accessing preventive care but their health beliefs and practices make their needs for care unique from other young adults.展开更多
Background and Aims:Metabolic dysfunction-associated steatotic liver disease(MASLD)is a leading cause of hepatic fibrosis,yet its prevalence in asymptomatic populations remains unclear.This study aimed to assess the p...Background and Aims:Metabolic dysfunction-associated steatotic liver disease(MASLD)is a leading cause of hepatic fibrosis,yet its prevalence in asymptomatic populations remains unclear.This study aimed to assess the prevalence of steatosis and significant fibrosis in asymptomatic individuals without known liver disease in the Greater Vancouver Area.Methods:Interested individuals voluntarily registered online via the Canadian Liver Foundation website or by telephone.Inclusion criteria included age≥19 years,no known liver disease,and low alcohol intake(<30 g/day for men,<20 g/day for women).Demographic and clinical data were collected,and all participants underwent transient elastography after a 3-h fast.The study aimed to collect 4,500 analyzable scans while reflecting the region’s ethnic diversity.Results:A total of 4,193 participants were analyzed.The median age was 62 years,the median body mass index was 25.4,and 45%were male.Asian individuals comprised 42%of the cohort.Steatosis was present in 59.6%of participants,and 45.7%met diagnostic criteria for MASLD.Significant fibrosis(F2-F4)was found in 8.6%.Age,male sex,ethnicity,cardiac disease,diabetes,hypertension,and obesity were significantly associated with fibrosis.Logistic regression analysis confirmed age,weight,diabetes,dyslipidemia,hypertension,and obesity as independent predictors.Conclusions:A substantial proportion of asymptomatic individuals in Greater Vancouver have undetected MASLD and significant fibrosis.Early identification of high-risk groups may support broader implementation of transient elastography screening.This study provides one of the first North American populationbased estimates of MASLD and fibrosis stratified by ethnicity,offering new insights into liver disease distribution among Caucasian,Chinese,and South Asian populations.展开更多
Central nervous system(CNS) axons fail to regenerate following brain or spinal cord injury(SCI),which typically leads to permanent neurological deficits.Peripheral nervous system axons,howeve r,can regenerate followin...Central nervous system(CNS) axons fail to regenerate following brain or spinal cord injury(SCI),which typically leads to permanent neurological deficits.Peripheral nervous system axons,howeve r,can regenerate following injury.Understanding the mechanisms that underlie this difference is key to developing treatments for CNS neurological diseases and injuries characterized by axonal damage.To initiate repair after peripheral nerve injury,dorsal root ganglion(DRG) neurons mobilize a pro-regenerative gene expression program,which facilitates axon outgrowth.展开更多
The inter-related pathological cascades following a traumatic spinal cord injury(tSCI)disrupt multiple cell types and physiological processes.Subsequently,motor and sensory functions are disrupted by breakdowns in cel...The inter-related pathological cascades following a traumatic spinal cord injury(tSCI)disrupt multiple cell types and physiological processes.Subsequently,motor and sensory functions are disrupted by breakdowns in cellular interactions and circuitry.Therapeutic interventions seek to modify some aspects of the injury course to enable the re-establishment of functional circuitry.Interventions often target one cell type(e.g.,promoting neuroprotection or neural regeneration)or one process(e.g.,modulating inflammation,affecting astrocytic,microglial,or macrophage responses.)Many axons in the spinal cord are myelinated,and after injury oligodendrocyte death causes demyelination.Promoting remyelination of spared or new axons to re-establish conduction seems a logical choice as a therapeutic target.展开更多
A fairly precise vision of a healthy physical self can serve as motivation for undertaking the means to that end.The same cannot be said with regard to“healthy moral selves”.By definition,democracy is about living w...A fairly precise vision of a healthy physical self can serve as motivation for undertaking the means to that end.The same cannot be said with regard to“healthy moral selves”.By definition,democracy is about living with others and,as we argue,a healthy moral self is one that lives well with others.However,precisely what“living with others”entails is ambiguous,particularly in a capitalist economy that presumes that the greatest happiness results from antagonistic competitiveness.In an attempt to demystify how self-focused individuals may nonetheless thrive in“the space between”,we will examine,with the help of Kant and Foucault,the Enlightenment Project that promoted maximal reasonableness(or what we are referring to as“healthy moral selves”)and then,with the help of Steven Pinker and Alisdair MacIntyre,explore the factors that have led to its seeming relatively recent failure.We will argue,following Iris Murdoch and others,that the best hope for its revitalization,and with it,democracy,lies,on one hand,with the debunking of counterfeit moral selves who use a“moral stance”to deliver what Frankfurt refers to as“bullshit”,and,on the other,with the reinvigoration of our understanding that healthy moral selves require a steady diet of engaging in“objective practical reasoning”with those who think differently,thereby potentially starving our fat relentless egos that are so often the source of divisiveness and,in so doing,become happy by becoming worthy of being happy.In animating the value of this goal,the hope is that the means,i.e.,education for the reinvigoration of practical reason(the forgotten twin of theoretical reason)and genuine deliberative dialogue across difference will become sufficiently attractive that it will energize democratic practice and dialogue to such an extent that democracy,as a form of government,may yet flourish despite the atomizing forces of capitalism.展开更多
The rise in urbanization has increasingly restricted access to natural environments,posing substantial risks to the physical and mental health of urban populations,including university students and other high-stress g...The rise in urbanization has increasingly restricted access to natural environments,posing substantial risks to the physical and mental health of urban populations,including university students and other high-stress groups.This study examines the comparative effects of outdoor forest meditation(OFM)and indoor nature meditation(INM)in simulated nature environments(SNEs)on the physiological and psychological health of university students.A pretestposttest repeated measures design was employed,with 40students participating in three replicated OFM sessions and three identical INM sessions across varied SNE settings.Key physiological metrics,including heart rate(HR),blood pressure(BP),and salivary amylase concentration(SAC),were measured before and after each session.Psychological well-being was assessed using the Perceived Stress Scale-10(PSS-10)and Profile of Mood States(POMS).Results revealed significant reductions(p<0.05)in most post-intervention outcomes,except in the second indoor session across physiologic al and psychologic al responses,while multi-sensory INM sessions produced comparable benefits.Notably,SNEs with enhanced sensory components were effective,though slightly less impactful than OFM.These findings suggest that both OFM in nature and INM in SNEs can benefit university students'well-being.INM in SNEs offers a promising alternative for those with limited access to natural settings,contributing meaningfully to stress reduction and overall well-being.This study highlights the potential for nature-based strategies for human health in urban centres,advocating for further investigation into the long-term impacts of SNEs and optimal sensory configurations for maximizing therapeutic effects in urban populations.展开更多
High-entropy materials(HEMs)have attracted extensive attention in the field of electrocatalysis due to their high performance enabled by their multi-component,tunable structural characteristics and excellent stability...High-entropy materials(HEMs)have attracted extensive attention in the field of electrocatalysis due to their high performance enabled by their multi-component,tunable structural characteristics and excellent stability.HEMs are usually composed of five or more metal elements,and have core advantages such as high configurational entropy,lattice distortion and multi-element synergistic effect,which provide new possibilities for composition regulation and performance optimization of catalysts.Especially at the nanoscale,HEMs show a larger specific surface area,abundant active sites and higher catalytic reaction efficiency,further expanding their application potential in electrochemical reactions.This paper systematically reviews the classification,structure construction and regulation strategies of HEMs,and focuses on their research progress in critical electrocatalytic reactions including water splitting(HER,OER),hydrogen oxidation(HOR),oxygen reduction(ORR),carbon dioxide reduction(CO_(2)RR),nitrate reduction(NO_(3)-RR)and electrooxidation of organics(EOO).In addition,the preparation methods of HEMs,the structure-performance relationship and the entropy regulation mechanism in the catalytic process are analyzed.Finally,this paper proposes the key challenges currently faced by HEMs in electrocatalytic applications and looks forward to their future development direction,providing a theoretical basis and design ideas for building a new generation of efficient and sustainable electrocatalysts.展开更多
While methodology for determining the mode of evolution in coding sequences has been well established,evaluation of adaptation events in emerging types of phenotype data needs further development.Here,we propose an an...While methodology for determining the mode of evolution in coding sequences has been well established,evaluation of adaptation events in emerging types of phenotype data needs further development.Here,we propose an analysis framework(expression variance decomposition,EVaDe)for comparative single-cell expression data based on phenotypic evolution theory.After decomposing the gene expression variance into separate components,we use two strategies to identify genes exhibiting large between-taxon expression divergence and small within-cell-type expression noise in certain cell types,attributing this pattern to putative adaptive evolution.In a dataset of primate prefrontal cortex,we find that such humanspecific key genes enrich with neurodevelopment-related functions,while most other genes exhibit neutral evolution patterns.Specific neuron types are found to harbor more of these key genes than other cell types,thus likely to have experienced more extensive adaptation.Reassuringly,at the molecular sequence level,the key genes are significantly associated with the rapidly evolving conserved non-coding elements.An additional case analysis comparing the naked mole-rat(NMR)with the mouse suggests that innateimmunity-related genes and cell types have undergone putative expression adaptation in NMR.Overall,the EVaDe framework may effectively probe adaptive evolution mode in single-cell expression data.展开更多
Lhasa,one of the world's highest cities,confronts the challenge of harmonizing cultural heritage preservation with ecological protection.Assessing the spatiotemporal dynamics of ecosystem service value(ESV)in its ...Lhasa,one of the world's highest cities,confronts the challenge of harmonizing cultural heritage preservation with ecological protection.Assessing the spatiotemporal dynamics of ecosystem service value(ESV)in its central urban area is therefore critical for informing future urban planning and land management.This study systematically analyzed land use evolution,the spatiotemporal characteristics of ecosystem services,and ecological network construction within Lhasa's central urban area.It integrated multi-source data,including Landsat remote sensing imagery from 2000,2010,and 2023,with multiple modeling methods such as the InVEST model,MaxEnt for cultural service assessment,the Minimum Cumulative Resistance(MCR)model,and circuit theory.Based on these analyses,optimization strategies were proposed.The results indicate that from 2000 to 2023,areas of cultivated land,grassland,and water bodies decreased by 7.47%,6.85%,and 0.68%,respectively,while wetland and forest areas expanded by 1.44%and 0.64%.Construction land exhibited significant expansion(12.94%),leading to an overall ESV reduction of 462.8×10^(5)yuan.Vegetation coverage was identified as the pivotal factor influencing ESV distribution,with higher values concentrated in the Lhasa River Basin and near the Lhalu Wetland,diminishing towards the urban core.Furthermore,spatial autocorrelation analysis revealed significant positive spatial clustering,with low-low aggregation in the eastern and central regions and high-high aggregation in the Lhasa River Basin and its surrounding water bodies.Moreover,based on a comprehensive ecosystem service assessment,11 ecological source sites were identified,primarily in the southwestern mountains and northeastern foothills.A comprehensive resistance surface,incorporating factors such as elevation,Normalized Difference Vegetation Index(NDVI),and land use,facilitated the extraction of 23 potential ecological corridors totaling 124.96 km in length.Topological network analysis indicated high redundancy and connectivity;however,marginal source sites relying on single connections exhibited significant vulnerability to rupture.Additionally,the application of circuit theory identified 30 ecological pinch points(current density≥1.5 A/km^(2))and 23 obstacle points,revealing significant blockages to ecological flow along the Qinghai-Xizang Highway,within the old city,and in other areas of high-intensity human activity.To address the identified network deficiencies—‘scattered cores,fragmented corridors,and insufficient resilience’—this study proposes an optimization strategy conceptualized as‘one vein,three corridors,and multiple cores’.Recommendations for enhancing network resilience include the delineation of ecological protection red lines,the integration of plateau-adapted technologies,and the fostering of community governance mechanisms.This approach aims to provide a scientific basis for constructing an ecological security pattern and promoting sustainable development in plateau cities.Ultimately,this research contributes to the enhancement of ecological well-being in the Himalayan region.展开更多
Dear Editor,This letter presents a new approach to developing interpretable and reliable soft sensors for Industry 5.0 applications.Although sophisticated machine learning methods have made remarkable strides in soft-...Dear Editor,This letter presents a new approach to developing interpretable and reliable soft sensors for Industry 5.0 applications.Although sophisticated machine learning methods have made remarkable strides in soft-sensor predictive accuracy,ensuring interpretability and reliable performance across varying industrial operating conditions remains a challenge[1]–[4].This is precisely what Industry 5.0,proposed by the European Commission in 2021,advocates[5],[6].It integrates various cutting-edge technologies,such as human-machine interaction,digital twins,cybersecurity and artificial intelligence,to facilitate the development of better soft sensors.展开更多
Climate warming is significantly altering the distribution of tree species,which holds crucial implications for China’s Larix species as they are important afforestation efforts.Understanding their optimal habitats a...Climate warming is significantly altering the distribution of tree species,which holds crucial implications for China’s Larix species as they are important afforestation efforts.Understanding their optimal habitats and environmental constraints is vital for predicting range shifts and guiding adaptive forest management.Previous studies prioritized changing climate impacts on horizontal range shifts of Larix,neglecting the influence of soil factors and range shift along altitudinal gradients.To address this,we applied an optimized MaxEnt model to assess current and future SSP126/SSP585 scenarios,three-dimensional habitat suitability(latitude,longitude,altitude)for four major Larix species(L.principis-rupprechtii,L.gmelinii,L.kaempferi,L.olgensis),while identifying key environmental drivers.Our results indicate that elevation and extreme moisture conditions universally constrain their distribution.Soil chemistry properties exhibited species-specific influences:cation exchange capacity critically shaped L.principis-rupprechtii and L.gmelinii ranges,whereas exchangeable aluminum determined L.kaempferi and L.olgensis distribution.Under future climate scenarios,habitat areas show divergent trajectories-L.principis-rupprechtii maximum gains 5.1%under SSP126,while L.kaempferi maximum expands 15.1%.Conversely,SSP585 triggered a 3.7% decline for L.gmelinii during the 2040s−2100s,and L.olgensis faces a net reduction to 0.4% by 2100s despite transient gains.Spatially,three species(L.kaempferi,L.gmelinii,L.olgensis)shifted northward,while L.principis-rupprechtii migrated northwest.All species distribution ascended altitudinally reflecting thermal adaptation strategies.These multidimensional insights enable targeted species selection for climate-resilient afforestation and underscore the need for soil-inclusive management planning.展开更多
During deep coal mining,an instability failure of coal usually occurs under the combined effect of initial damage and triaxial cyclic loading and unloading(TCLU).Therefore,this study investigated the impact of initial...During deep coal mining,an instability failure of coal usually occurs under the combined effect of initial damage and triaxial cyclic loading and unloading(TCLU).Therefore,this study investigated the impact of initial damage on mechanical behavior and acoustic emission(AE)characteristics of coal under TCLU.Initial damage variables(IDVs)of coal specimens were quantified using preloading,followed by TCLU experiments to assess the deformation,energy distribution,and fracture development.The results revealed that the increase in IDVs significantly reduced the structural integrity of coal specimens,increased the cumulative irreversible strain,and enhanced the dissipated energy owing to microfracture expansion.Moreover,AE monitoring showed earlier activation of fractures and a higher occurrence of large-scale rupture events of coal specimens with high IDVs,which correlated with decreasing AE b values(reflecting the different scales of fracture within specimens)and increasing S values(reflecting the AE activity within specimens).Additionally,computed tomography analysis revealed intensified fracture networks and increasing three-dimensional fractal dimensions of coal specimens with higher IDVs.Finally,the coupling effect of TCLU and initial damage on the weakening mechanism of coal was investigated.Initial damage significantly reduced the structural integrity of coal by increasing the number of weak planes within coal specimens,contributing to the earlier activation and rapid expansion of fractures at low stress levels under TCLU and eventually accelerating the weakening process of coal.This study provides a scientific basis and theoretical support for the prevention and control of dynamic disasters in deep coal mining.展开更多
Multivitamins were widely used health supplements that replenished essential nutrients in the human body.Despite their popularity,the impact of multivitamins on the cognitive function of older adults remained unclear ...Multivitamins were widely used health supplements that replenished essential nutrients in the human body.Despite their popularity,the impact of multivitamins on the cognitive function of older adults remained unclear and contentious.This study offered a comprehensive review and meta-analysis of research published until June 2024,analyzing the effects of multivitamins on various cognitive functions in individuals aged 65 and older.We included ten randomized controlled trials encompassing 13,600 participants from multiple databases.These studies evaluated the impact of multivitamins on reasoning,memory,learning,visual perception,idea production,cognitive speed,psychomotor abilities,and higher cognitive functions.Our meta-analysis revealed that multivitamins significantly enhanced delayed free recall (standardized mean difference(SMD)=0.09,95%confidence interval(CI)=[0.05,0.13],P<0.0001).However,they had no substantial effects on immediate free recall(SMD=0.85,95%CI=[-0.18,1.90],P=0.11),idea production(SMD=0.00,95%CI=[-0.04,0.03],P=0.86),or cognitive speed(SMD=0.34,95%CI=[-0.07,0.74],P=0.11).Thus,while multivitamins facilitated delayed free recall,they did not significantly improve other cognitive functions in older adults.展开更多
After coal seam mining,the overlying rock strata above the goaf are subjected to long-term stress and eventually undergo failure.Under mining-induced disturbances,the strata develop fractures at various angles,which s...After coal seam mining,the overlying rock strata above the goaf are subjected to long-term stress and eventually undergo failure.Under mining-induced disturbances,the strata develop fractures at various angles,which significantly influence failure modes and the morphology of gas flow channels.This study employed multistage loading experiments,numerical simulations,three-dimensional reconstruction,and image recognition to investigate the fragmentation process of rocks with different initial fracture angles under multistage loading.The results show that variations in the initial fracture angle affect the transmission of contact forces among rock particles.As the angle increases,the transmission pattern shifts from a uniform distribution to one extending along the direction of the fracture.Rocks with small initial fracture angles tend to experience tensile-dominated failure,with most of the material subjected to longitudinal loading,resulting in reduced strength.Fractures propagate from the central region of the initial fracture,producing a complex internal fracture network.The proportion of fracture channels varies considerably across regions,creating multiple zones of velocity variation in the gas flow.In contrast,rocks with large initial fracture angles are more susceptible to shear failure,with the primary load-bearing zones aligned along the inclined fracture direction.As a result,the influence on surrounding regions is limited,improving the rock's load-bearing capacity under multistage loading.In these cases,the distribution and proportion of fracture channels become more uniform,promoting more stable gas flow within the channels.Overall,these findings provide theoretical insights into how initial fracture angles govern rock failure patterns and gas flow characteristics.展开更多
Background The enteric methane inhibitor 3-nitrooxypropanol(3-NOP)inhibits the key enzyme in ruminal methanogenesis,but whether short-term(ST)and long-term(LT)dietary supplementation has similar effects on rumen micro...Background The enteric methane inhibitor 3-nitrooxypropanol(3-NOP)inhibits the key enzyme in ruminal methanogenesis,but whether short-term(ST)and long-term(LT)dietary supplementation has similar effects on rumen microbiota in beef cattle and how microbes change after 3-NOP withdrawal have not been studied.This study investigated changes in rumen bacteria,archaea,and protozoa after ST and LT dietary supplementation and removal of 3-NOP using metataxonomic analysis.Results A total of 143 rumen samples were collected from two beef cattle studies with 3-NOP supplementation.The ST study(95 samples)used eight ruminally cannulated beef cattle in a 4×4 Latin square design with four 28-d of 3-NOP treatments[mg/kg of dry matter(DM)]:control:0,low:53,med:161,and high:345.The LT study(48 samples)was a completely randomized design with two 3-NOP treatments[control:0,and high:280 mg/kg of DM)fed for 112-d followed by a 16-d withdrawal(without 3-NOP).Bacterial and archaeal communities were significantly affected by 3-NOP supplementation but limited effects on protozoal communities were observed.Under ST supplementation,the relative abundances of Prevotella,Methanobrevibacter(Mbb.)ruminantium,Methanosphaera sp.ISO3-F5,and Entodinium were increased(Q<0.05),whereas those of Mbb.gottschalkii and Epidinium were decreased(Q<0.05)with 3-NOP supplementation.In LT study,relative abundances of Mbb.ruminantium,and Methanosphaera sp.Group5 were increased(Q<0.05),while those of Saccharofermentans and Mbb.gottschalkii were decreased(Q<0.05)with 3-NOP supplementation.Comparison between 3-NOP supplementation and the withdrawal revealed increased relative abundances of Clostridia UCG-014 and Oscillospiraceae NK4A214 group and decreased those of Eubacterium nodatum group and Methanosphaera sp.Group5(P<0.05)after 3-NOP withdrawal.Further comparison of rumen microbiota between control and 3-NOP withdrawal showed significantly higher(P=0.029)relative abundances of Eggerthellaceae DNF00809,p-1088-a5 gut group,and Family XII UCG-001 in control group while no significant differences were detected for archaea and protozoa.Microbial network analysis revealed that microbial interactions differed by both 3-NOP dose and durations.Conclusions Both ST and LT supplementation affected overall rumen microbial profile,with individual microbial groups responded to 3-NOP supplementation differently.After 3-NOP withdrawal,not all microbes showed recovery,indicating that the 3-NOP driven shifts were only partially reversible.These findings provide an understanding of the effects of 3-NOP on rumen microbial communities and their adaptability to methane mitigation strategies.展开更多
Physician well-being is vital to delivering high-quality emergency care.A supported and healthy emergency medicine workforce leads to better patient outcomes,fewer medical errors,and greater job satisfaction and staff...Physician well-being is vital to delivering high-quality emergency care.A supported and healthy emergency medicine workforce leads to better patient outcomes,fewer medical errors,and greater job satisfaction and staff retention.[1,2]Emergency physicians(EPs)face unique pressures,including shift work,high patient volumes and acuities,overcrowding,and systemic inefficiencies that escalate their risk of burnout.As a result,EPs have reported the highest rates of burnout among physician specialties.展开更多
文摘Stable isotope analysis was used to determine the relative dietary importance of kelp-derived detritus to plankton and benthic organisms along a gradient of kelp abundance driven by recovering sea otter populations along the west coast of Vancouver Island (WCVI), Canada. The study used region-specific kelp isotope values (δ<sup>13</sup>C and δ<sup>15</sup>N) and season-specific phytoplankton isotope values to model dietary contributions of kelp-derived detritus (KDD). In general, KDD contributions were moderate to high in most plankton size fractions during the summer and decreased during the winter, particularly in the kelp sparse region. Hypothesized regional and spatial (distance from the coast) differences in kelp detritus contributions to zooplankton w<span style="white-space:normal;"><span style="font-family:;" "="">ere<span style="white-space:normal;"><span style="font-family:;" "=""> not evident. Modeled estimates of the KDD contribution to benthic invertebrates w<span style="white-space:normal;"><span style="font-family:;" "="">ere<span style="white-space:normal;"><span style="font-family:;" "=""> high (>40%) and independent of the organism size, among regions and between seasons, with the exception of Astraea gibberosa in the kelp abundant region. Local oceanography, natural kelp isotope signature variation, and significant overlap between kelps’ and blooming phytoplankton isotope values led to a large uncertainty in the assessed KDD contributions in benthic organisms. These results highlighted the importance of the KDD as a widespread and stable year-round food source in coastal kelp populated regions.
文摘Background: The distribution of forest vegetation within urban environments is critically important as it influences urban environmental conditions and the energy exchange through the absorption of solar radiation and modulation of evapotranspiration. It also plays an important role filtering urban water systems and reducing storm water runoff.Methods: We investigate the capacity of ALS data to individually detect, map and characterize large(taller than15 m) trees within the City of Vancouver. Large trees are critical for the function and character of Vancouver’s urban forest. We used an object-based approach for individual tree detection and segmentation to determine tree locations(position of the stem), to delineate the shape of the crowns and to categorize the latter either as coniferous or deciduous.Results: Results indicate a detection rate of 76.6% for trees > 15 m with a positioning error of 2.11 m(stem location). Extracted tree heights possessed a RMSE of 2.60 m and a bias of-1.87 m, whereas crown diameter was derived with a RMSE of 3.85 m and a bias of-2.06 m. Missed trees are principally a result of undetected treetops occurring in dense, overlapping canopies with more accurate detection and delineation of trees in open areas.Conclusion: By identifying key structural trees across Vancouver’s urban forests, we can better understand their role in providing ecosystem goods and services for city residents.
文摘The province of British Columbia, Canada, has established an Agricultural Land Reserve (ALR) to protect the most suitable soil landscapes for agriculture. Increases in population and urbanization have resulted in development challenges on ALR lands. The Metro Vancouver area is the most productive agricultural area in British Columbia as well as the most rapidly growing urban region. The increase in impervious areas has decreased the amount of arable land for soil-based agriculture and altered the hydrological cycle. Analysis using a combination of aerial imagery and GIS found that impervious areas comprise about 10 percent of the ALR within Metro Vancouver. Farm residences and greenhouses have the largest effect on reducing the soil surface for water infiltration. This decrease in area has negatively influenced the ecosystem heath of the region, as well as, decreasing the amount of agricultural land for soil based agriculture and both surface and groundwater dynamics.
文摘Objectives: Immigrants of Chinese ethnicity and young people (between 18 and 30 years of age) are known to access health services less frequently and may be at greater risk for experiencing unmet health needs. The purpose of this study was to examine the health beliefs, health behaviors, primary care access, and perceived unmet healthcare needs of Chinese young adults.Methods: Semi-structured in-depth interviews were carried out with eight Chinese young adults in Vancouver, Canada. Results: A content analysis revealed that these Chinese young adults experienced unmet healthcare needs, did not have a primary care provider, and did not access preventive services. Cultural factors such as strong family ties, filial piety, and the practice of Traditional Chinese Medicine influenced their health behaviors and healthcare access patterns. Conclusion: Chinese young adults share similar issues with other young adults in relation to not having a primary care provider and accessing preventive care but their health beliefs and practices make their needs for care unique from other young adults.
文摘Background and Aims:Metabolic dysfunction-associated steatotic liver disease(MASLD)is a leading cause of hepatic fibrosis,yet its prevalence in asymptomatic populations remains unclear.This study aimed to assess the prevalence of steatosis and significant fibrosis in asymptomatic individuals without known liver disease in the Greater Vancouver Area.Methods:Interested individuals voluntarily registered online via the Canadian Liver Foundation website or by telephone.Inclusion criteria included age≥19 years,no known liver disease,and low alcohol intake(<30 g/day for men,<20 g/day for women).Demographic and clinical data were collected,and all participants underwent transient elastography after a 3-h fast.The study aimed to collect 4,500 analyzable scans while reflecting the region’s ethnic diversity.Results:A total of 4,193 participants were analyzed.The median age was 62 years,the median body mass index was 25.4,and 45%were male.Asian individuals comprised 42%of the cohort.Steatosis was present in 59.6%of participants,and 45.7%met diagnostic criteria for MASLD.Significant fibrosis(F2-F4)was found in 8.6%.Age,male sex,ethnicity,cardiac disease,diabetes,hypertension,and obesity were significantly associated with fibrosis.Logistic regression analysis confirmed age,weight,diabetes,dyslipidemia,hypertension,and obesity as independent predictors.Conclusions:A substantial proportion of asymptomatic individuals in Greater Vancouver have undetected MASLD and significant fibrosis.Early identification of high-risk groups may support broader implementation of transient elastography screening.This study provides one of the first North American populationbased estimates of MASLD and fibrosis stratified by ethnicity,offering new insights into liver disease distribution among Caucasian,Chinese,and South Asian populations.
基金supported by the Canada Foundation for Innovation (Project#44220)the Natural Sciences and Engineering Research Council of Canada (RGPIN-2024-03986)+3 种基金the Michael Smith Foundation for Health Research BCthe financial support of Health Canada,through the Canada Brain Research Fund,an innovative partnership between the Government of Canada (through Health Canada),Brain Canada Foundationthe Azrieli Foundationsupported by a Canadian Institutes of Health Research (CIHR) Canada Graduate Scholarship–Master’s Award。
文摘Central nervous system(CNS) axons fail to regenerate following brain or spinal cord injury(SCI),which typically leads to permanent neurological deficits.Peripheral nervous system axons,howeve r,can regenerate following injury.Understanding the mechanisms that underlie this difference is key to developing treatments for CNS neurological diseases and injuries characterized by axonal damage.To initiate repair after peripheral nerve injury,dorsal root ganglion(DRG) neurons mobilize a pro-regenerative gene expression program,which facilitates axon outgrowth.
基金supported by Grant 3195 from Paralyzed Veterans of America Research Foundation (to BRK)
文摘The inter-related pathological cascades following a traumatic spinal cord injury(tSCI)disrupt multiple cell types and physiological processes.Subsequently,motor and sensory functions are disrupted by breakdowns in cellular interactions and circuitry.Therapeutic interventions seek to modify some aspects of the injury course to enable the re-establishment of functional circuitry.Interventions often target one cell type(e.g.,promoting neuroprotection or neural regeneration)or one process(e.g.,modulating inflammation,affecting astrocytic,microglial,or macrophage responses.)Many axons in the spinal cord are myelinated,and after injury oligodendrocyte death causes demyelination.Promoting remyelination of spared or new axons to re-establish conduction seems a logical choice as a therapeutic target.
文摘A fairly precise vision of a healthy physical self can serve as motivation for undertaking the means to that end.The same cannot be said with regard to“healthy moral selves”.By definition,democracy is about living with others and,as we argue,a healthy moral self is one that lives well with others.However,precisely what“living with others”entails is ambiguous,particularly in a capitalist economy that presumes that the greatest happiness results from antagonistic competitiveness.In an attempt to demystify how self-focused individuals may nonetheless thrive in“the space between”,we will examine,with the help of Kant and Foucault,the Enlightenment Project that promoted maximal reasonableness(or what we are referring to as“healthy moral selves”)and then,with the help of Steven Pinker and Alisdair MacIntyre,explore the factors that have led to its seeming relatively recent failure.We will argue,following Iris Murdoch and others,that the best hope for its revitalization,and with it,democracy,lies,on one hand,with the debunking of counterfeit moral selves who use a“moral stance”to deliver what Frankfurt refers to as“bullshit”,and,on the other,with the reinvigoration of our understanding that healthy moral selves require a steady diet of engaging in“objective practical reasoning”with those who think differently,thereby potentially starving our fat relentless egos that are so often the source of divisiveness and,in so doing,become happy by becoming worthy of being happy.In animating the value of this goal,the hope is that the means,i.e.,education for the reinvigoration of practical reason(the forgotten twin of theoretical reason)and genuine deliberative dialogue across difference will become sufficiently attractive that it will energize democratic practice and dialogue to such an extent that democracy,as a form of government,may yet flourish despite the atomizing forces of capitalism.
基金funded by UBC Forest and Human Wellbeing Research(Grant No.GR020223)。
文摘The rise in urbanization has increasingly restricted access to natural environments,posing substantial risks to the physical and mental health of urban populations,including university students and other high-stress groups.This study examines the comparative effects of outdoor forest meditation(OFM)and indoor nature meditation(INM)in simulated nature environments(SNEs)on the physiological and psychological health of university students.A pretestposttest repeated measures design was employed,with 40students participating in three replicated OFM sessions and three identical INM sessions across varied SNE settings.Key physiological metrics,including heart rate(HR),blood pressure(BP),and salivary amylase concentration(SAC),were measured before and after each session.Psychological well-being was assessed using the Perceived Stress Scale-10(PSS-10)and Profile of Mood States(POMS).Results revealed significant reductions(p<0.05)in most post-intervention outcomes,except in the second indoor session across physiologic al and psychologic al responses,while multi-sensory INM sessions produced comparable benefits.Notably,SNEs with enhanced sensory components were effective,though slightly less impactful than OFM.These findings suggest that both OFM in nature and INM in SNEs can benefit university students'well-being.INM in SNEs offers a promising alternative for those with limited access to natural settings,contributing meaningfully to stress reduction and overall well-being.This study highlights the potential for nature-based strategies for human health in urban centres,advocating for further investigation into the long-term impacts of SNEs and optimal sensory configurations for maximizing therapeutic effects in urban populations.
基金supported by the National Natural Science Foundation of China(22378247 and 22078187)China-CEEC University Joint Education Project(2021099)+1 种基金the International Joint Research Center for Biomass Chemistry and Materials,the Shaanxi International Science and Technology Cooperation Base(2018GHJD-19)Ning Wei and Xue Yao are grateful to Innovative Talents International Cooperative Training Project from China Scholarship Council(Grant No.202310470014 and 202310470013).
文摘High-entropy materials(HEMs)have attracted extensive attention in the field of electrocatalysis due to their high performance enabled by their multi-component,tunable structural characteristics and excellent stability.HEMs are usually composed of five or more metal elements,and have core advantages such as high configurational entropy,lattice distortion and multi-element synergistic effect,which provide new possibilities for composition regulation and performance optimization of catalysts.Especially at the nanoscale,HEMs show a larger specific surface area,abundant active sites and higher catalytic reaction efficiency,further expanding their application potential in electrochemical reactions.This paper systematically reviews the classification,structure construction and regulation strategies of HEMs,and focuses on their research progress in critical electrocatalytic reactions including water splitting(HER,OER),hydrogen oxidation(HOR),oxygen reduction(ORR),carbon dioxide reduction(CO_(2)RR),nitrate reduction(NO_(3)-RR)and electrooxidation of organics(EOO).In addition,the preparation methods of HEMs,the structure-performance relationship and the entropy regulation mechanism in the catalytic process are analyzed.Finally,this paper proposes the key challenges currently faced by HEMs in electrocatalytic applications and looks forward to their future development direction,providing a theoretical basis and design ideas for building a new generation of efficient and sustainable electrocatalysts.
文摘While methodology for determining the mode of evolution in coding sequences has been well established,evaluation of adaptation events in emerging types of phenotype data needs further development.Here,we propose an analysis framework(expression variance decomposition,EVaDe)for comparative single-cell expression data based on phenotypic evolution theory.After decomposing the gene expression variance into separate components,we use two strategies to identify genes exhibiting large between-taxon expression divergence and small within-cell-type expression noise in certain cell types,attributing this pattern to putative adaptive evolution.In a dataset of primate prefrontal cortex,we find that such humanspecific key genes enrich with neurodevelopment-related functions,while most other genes exhibit neutral evolution patterns.Specific neuron types are found to harbor more of these key genes than other cell types,thus likely to have experienced more extensive adaptation.Reassuringly,at the molecular sequence level,the key genes are significantly associated with the rapidly evolving conserved non-coding elements.An additional case analysis comparing the naked mole-rat(NMR)with the mouse suggests that innateimmunity-related genes and cell types have undergone putative expression adaptation in NMR.Overall,the EVaDe framework may effectively probe adaptive evolution mode in single-cell expression data.
基金National Natural Science Foundation of China Youth Fund Project:Research on the Construction of Ecological Security Pattern in the Transition Zone of Nature Reserves along the Sichuan-Xizang Railway(Western Sichuan Section)(51908470).
文摘Lhasa,one of the world's highest cities,confronts the challenge of harmonizing cultural heritage preservation with ecological protection.Assessing the spatiotemporal dynamics of ecosystem service value(ESV)in its central urban area is therefore critical for informing future urban planning and land management.This study systematically analyzed land use evolution,the spatiotemporal characteristics of ecosystem services,and ecological network construction within Lhasa's central urban area.It integrated multi-source data,including Landsat remote sensing imagery from 2000,2010,and 2023,with multiple modeling methods such as the InVEST model,MaxEnt for cultural service assessment,the Minimum Cumulative Resistance(MCR)model,and circuit theory.Based on these analyses,optimization strategies were proposed.The results indicate that from 2000 to 2023,areas of cultivated land,grassland,and water bodies decreased by 7.47%,6.85%,and 0.68%,respectively,while wetland and forest areas expanded by 1.44%and 0.64%.Construction land exhibited significant expansion(12.94%),leading to an overall ESV reduction of 462.8×10^(5)yuan.Vegetation coverage was identified as the pivotal factor influencing ESV distribution,with higher values concentrated in the Lhasa River Basin and near the Lhalu Wetland,diminishing towards the urban core.Furthermore,spatial autocorrelation analysis revealed significant positive spatial clustering,with low-low aggregation in the eastern and central regions and high-high aggregation in the Lhasa River Basin and its surrounding water bodies.Moreover,based on a comprehensive ecosystem service assessment,11 ecological source sites were identified,primarily in the southwestern mountains and northeastern foothills.A comprehensive resistance surface,incorporating factors such as elevation,Normalized Difference Vegetation Index(NDVI),and land use,facilitated the extraction of 23 potential ecological corridors totaling 124.96 km in length.Topological network analysis indicated high redundancy and connectivity;however,marginal source sites relying on single connections exhibited significant vulnerability to rupture.Additionally,the application of circuit theory identified 30 ecological pinch points(current density≥1.5 A/km^(2))and 23 obstacle points,revealing significant blockages to ecological flow along the Qinghai-Xizang Highway,within the old city,and in other areas of high-intensity human activity.To address the identified network deficiencies—‘scattered cores,fragmented corridors,and insufficient resilience’—this study proposes an optimization strategy conceptualized as‘one vein,three corridors,and multiple cores’.Recommendations for enhancing network resilience include the delineation of ecological protection red lines,the integration of plateau-adapted technologies,and the fostering of community governance mechanisms.This approach aims to provide a scientific basis for constructing an ecological security pattern and promoting sustainable development in plateau cities.Ultimately,this research contributes to the enhancement of ecological well-being in the Himalayan region.
文摘Dear Editor,This letter presents a new approach to developing interpretable and reliable soft sensors for Industry 5.0 applications.Although sophisticated machine learning methods have made remarkable strides in soft-sensor predictive accuracy,ensuring interpretability and reliable performance across varying industrial operating conditions remains a challenge[1]–[4].This is precisely what Industry 5.0,proposed by the European Commission in 2021,advocates[5],[6].It integrates various cutting-edge technologies,such as human-machine interaction,digital twins,cybersecurity and artificial intelligence,to facilitate the development of better soft sensors.
基金supported by the National Key Research and Development Program of China(2022YFD2200501).
文摘Climate warming is significantly altering the distribution of tree species,which holds crucial implications for China’s Larix species as they are important afforestation efforts.Understanding their optimal habitats and environmental constraints is vital for predicting range shifts and guiding adaptive forest management.Previous studies prioritized changing climate impacts on horizontal range shifts of Larix,neglecting the influence of soil factors and range shift along altitudinal gradients.To address this,we applied an optimized MaxEnt model to assess current and future SSP126/SSP585 scenarios,three-dimensional habitat suitability(latitude,longitude,altitude)for four major Larix species(L.principis-rupprechtii,L.gmelinii,L.kaempferi,L.olgensis),while identifying key environmental drivers.Our results indicate that elevation and extreme moisture conditions universally constrain their distribution.Soil chemistry properties exhibited species-specific influences:cation exchange capacity critically shaped L.principis-rupprechtii and L.gmelinii ranges,whereas exchangeable aluminum determined L.kaempferi and L.olgensis distribution.Under future climate scenarios,habitat areas show divergent trajectories-L.principis-rupprechtii maximum gains 5.1%under SSP126,while L.kaempferi maximum expands 15.1%.Conversely,SSP585 triggered a 3.7% decline for L.gmelinii during the 2040s−2100s,and L.olgensis faces a net reduction to 0.4% by 2100s despite transient gains.Spatially,three species(L.kaempferi,L.gmelinii,L.olgensis)shifted northward,while L.principis-rupprechtii migrated northwest.All species distribution ascended altitudinally reflecting thermal adaptation strategies.These multidimensional insights enable targeted species selection for climate-resilient afforestation and underscore the need for soil-inclusive management planning.
基金supported by the National Key R&D Program of China(Grant No.2022YFC3004704)the National Natural Science Foundation of China(Grant No.52174166)Graduate Research and Innovation Foundation of Chongqing,China(Grant No.CYB23031),which were gratefully acknowledged.
文摘During deep coal mining,an instability failure of coal usually occurs under the combined effect of initial damage and triaxial cyclic loading and unloading(TCLU).Therefore,this study investigated the impact of initial damage on mechanical behavior and acoustic emission(AE)characteristics of coal under TCLU.Initial damage variables(IDVs)of coal specimens were quantified using preloading,followed by TCLU experiments to assess the deformation,energy distribution,and fracture development.The results revealed that the increase in IDVs significantly reduced the structural integrity of coal specimens,increased the cumulative irreversible strain,and enhanced the dissipated energy owing to microfracture expansion.Moreover,AE monitoring showed earlier activation of fractures and a higher occurrence of large-scale rupture events of coal specimens with high IDVs,which correlated with decreasing AE b values(reflecting the different scales of fracture within specimens)and increasing S values(reflecting the AE activity within specimens).Additionally,computed tomography analysis revealed intensified fracture networks and increasing three-dimensional fractal dimensions of coal specimens with higher IDVs.Finally,the coupling effect of TCLU and initial damage on the weakening mechanism of coal was investigated.Initial damage significantly reduced the structural integrity of coal by increasing the number of weak planes within coal specimens,contributing to the earlier activation and rapid expansion of fractures at low stress levels under TCLU and eventually accelerating the weakening process of coal.This study provides a scientific basis and theoretical support for the prevention and control of dynamic disasters in deep coal mining.
基金supported by the Fundamental Research Funds for the Central Universities(2042023gf0003)Hubei Provincial Natural Science Foundation of China(2024AFD126)National Key Research and Development Program of China(2023YFF1104404).
文摘Multivitamins were widely used health supplements that replenished essential nutrients in the human body.Despite their popularity,the impact of multivitamins on the cognitive function of older adults remained unclear and contentious.This study offered a comprehensive review and meta-analysis of research published until June 2024,analyzing the effects of multivitamins on various cognitive functions in individuals aged 65 and older.We included ten randomized controlled trials encompassing 13,600 participants from multiple databases.These studies evaluated the impact of multivitamins on reasoning,memory,learning,visual perception,idea production,cognitive speed,psychomotor abilities,and higher cognitive functions.Our meta-analysis revealed that multivitamins significantly enhanced delayed free recall (standardized mean difference(SMD)=0.09,95%confidence interval(CI)=[0.05,0.13],P<0.0001).However,they had no substantial effects on immediate free recall(SMD=0.85,95%CI=[-0.18,1.90],P=0.11),idea production(SMD=0.00,95%CI=[-0.04,0.03],P=0.86),or cognitive speed(SMD=0.34,95%CI=[-0.07,0.74],P=0.11).Thus,while multivitamins facilitated delayed free recall,they did not significantly improve other cognitive functions in older adults.
基金supported by the National Natural Science Foundation of China(Grant No.52522405)Key R&D Project of Sichuan Province of China(Regional Innovation Coop-eration)(Grant No.2025YFHZ0314).
文摘After coal seam mining,the overlying rock strata above the goaf are subjected to long-term stress and eventually undergo failure.Under mining-induced disturbances,the strata develop fractures at various angles,which significantly influence failure modes and the morphology of gas flow channels.This study employed multistage loading experiments,numerical simulations,three-dimensional reconstruction,and image recognition to investigate the fragmentation process of rocks with different initial fracture angles under multistage loading.The results show that variations in the initial fracture angle affect the transmission of contact forces among rock particles.As the angle increases,the transmission pattern shifts from a uniform distribution to one extending along the direction of the fracture.Rocks with small initial fracture angles tend to experience tensile-dominated failure,with most of the material subjected to longitudinal loading,resulting in reduced strength.Fractures propagate from the central region of the initial fracture,producing a complex internal fracture network.The proportion of fracture channels varies considerably across regions,creating multiple zones of velocity variation in the gas flow.In contrast,rocks with large initial fracture angles are more susceptible to shear failure,with the primary load-bearing zones aligned along the inclined fracture direction.As a result,the influence on surrounding regions is limited,improving the rock's load-bearing capacity under multistage loading.In these cases,the distribution and proportion of fracture channels become more uniform,promoting more stable gas flow within the channels.Overall,these findings provide theoretical insights into how initial fracture angles govern rock failure patterns and gas flow characteristics.
基金funded by the Beef Cattle Research Council Cluster(FDE.18.21C)Natural Sciences and Engineering Research Council of Canada(NSERC)Discovery,NSERC Canadian Research Chair(Tier 1)program+2 种基金NSERC Alliance program(ALLRP 588541‐23)Foundation for Food&Agriculture Research Greener Cattle Initiative(Award ID 22‐000373)DSM Nutritional Products,Kaiseraugst,Switzerland。
文摘Background The enteric methane inhibitor 3-nitrooxypropanol(3-NOP)inhibits the key enzyme in ruminal methanogenesis,but whether short-term(ST)and long-term(LT)dietary supplementation has similar effects on rumen microbiota in beef cattle and how microbes change after 3-NOP withdrawal have not been studied.This study investigated changes in rumen bacteria,archaea,and protozoa after ST and LT dietary supplementation and removal of 3-NOP using metataxonomic analysis.Results A total of 143 rumen samples were collected from two beef cattle studies with 3-NOP supplementation.The ST study(95 samples)used eight ruminally cannulated beef cattle in a 4×4 Latin square design with four 28-d of 3-NOP treatments[mg/kg of dry matter(DM)]:control:0,low:53,med:161,and high:345.The LT study(48 samples)was a completely randomized design with two 3-NOP treatments[control:0,and high:280 mg/kg of DM)fed for 112-d followed by a 16-d withdrawal(without 3-NOP).Bacterial and archaeal communities were significantly affected by 3-NOP supplementation but limited effects on protozoal communities were observed.Under ST supplementation,the relative abundances of Prevotella,Methanobrevibacter(Mbb.)ruminantium,Methanosphaera sp.ISO3-F5,and Entodinium were increased(Q<0.05),whereas those of Mbb.gottschalkii and Epidinium were decreased(Q<0.05)with 3-NOP supplementation.In LT study,relative abundances of Mbb.ruminantium,and Methanosphaera sp.Group5 were increased(Q<0.05),while those of Saccharofermentans and Mbb.gottschalkii were decreased(Q<0.05)with 3-NOP supplementation.Comparison between 3-NOP supplementation and the withdrawal revealed increased relative abundances of Clostridia UCG-014 and Oscillospiraceae NK4A214 group and decreased those of Eubacterium nodatum group and Methanosphaera sp.Group5(P<0.05)after 3-NOP withdrawal.Further comparison of rumen microbiota between control and 3-NOP withdrawal showed significantly higher(P=0.029)relative abundances of Eggerthellaceae DNF00809,p-1088-a5 gut group,and Family XII UCG-001 in control group while no significant differences were detected for archaea and protozoa.Microbial network analysis revealed that microbial interactions differed by both 3-NOP dose and durations.Conclusions Both ST and LT supplementation affected overall rumen microbial profile,with individual microbial groups responded to 3-NOP supplementation differently.After 3-NOP withdrawal,not all microbes showed recovery,indicating that the 3-NOP driven shifts were only partially reversible.These findings provide an understanding of the effects of 3-NOP on rumen microbial communities and their adaptability to methane mitigation strategies.
文摘Physician well-being is vital to delivering high-quality emergency care.A supported and healthy emergency medicine workforce leads to better patient outcomes,fewer medical errors,and greater job satisfaction and staff retention.[1,2]Emergency physicians(EPs)face unique pressures,including shift work,high patient volumes and acuities,overcrowding,and systemic inefficiencies that escalate their risk of burnout.As a result,EPs have reported the highest rates of burnout among physician specialties.
