Nitrogen（N） is considered as an important yield and quality-determining factor in soybean production.The objective of the current experiment was to determine the regularity of N accumulation and its simulation among...Nitrogen（N） is considered as an important yield and quality-determining factor in soybean production.The objective of the current experiment was to determine the regularity of N accumulation and its simulation among different varieties.This work was conducted with frame tests（pot without bottom）,and three cultivars,HH41,SN14,HN40,and one feed soybean variety MSD was used as materials.The results showed that the regularity of N accumulation in four varieties with the same tendency increased during the whole growth period,which was up to the maximum when the plant was harvested.The longer growth period was,the higher N accumulation of cultivars was,HN40〉SN14〉HH41,N accumulation of MSD was the highest because of the property for feed soybean.Regularity of N accumulation in plants could be accurately described by the Logistic equation.During the growth there was a period when N accumulation in the whole plant occurred at an intense rate.The time of onset and its duration varied with different varieties.The maximum daily N accumulation was on seed filling stage,pod bearing stage,seed filling stage and pod bearing stage in HH41,SN14,HN40 and MSD,respectively.展开更多
The β subunit of soybean [Glycine max (L.) Merr.] seed storage protein is of great significance in sulfur-containing amino acid balance and soybean processing properties. The objective of this study was to elucidate ...The β subunit of soybean [Glycine max (L.) Merr.] seed storage protein is of great significance in sulfur-containing amino acid balance and soybean processing properties. The objective of this study was to elucidate the relationship between the β subunit and sulfur-containing amino acid composition, and the potential regulatory mechanism. The β subunit was independently accumulated in comparison with other major subunits (α/α′, acidic, basic, and A3) during seed filling, and a low level of β subunit content (BSC) was formed during the accumulation process. In low-BSC mature seeds, crude protein, oil content, and fatty acid composition were not changed, but sulfur-containing amino acids (Cys + Met) in the low- BSC seeds increased significantly (by 31.5%), suggesting that an internal regulatory mechanism within seed might be responsible for the rebalance of seed protein composition and that sulfur assimilation might be deeply involved in β subunit accumulation. Transcriptomic analysis revealed that genes involved in anabolism of cysteine, methionine, and glutathione were up-regulated but those involved in the catabolism of these compounds were down-regulated, suggesting a relationship between the elevation of methionine and glutathione and low BSC. Our study sheds light on seed composition in low BSC lines and on the potential molecular regulatory mechanism of β subunit accumulation, broadening our understanding of soybean seed protein synthesis and its regulation.展开更多
为了探究β-伴大豆球蛋白(7S)中α和α′亚基缺失对大豆分离蛋白乳化特性的影响,该文以东农47(对照)和3种不同蛋白亚基缺失型(α缺失、α′缺失以及α、α′缺失)大豆为原料提取大豆分离蛋白(Soy Protein Isolate,SPI),通过十二烷基磺...为了探究β-伴大豆球蛋白(7S)中α和α′亚基缺失对大豆分离蛋白乳化特性的影响,该文以东农47(对照)和3种不同蛋白亚基缺失型(α缺失、α′缺失以及α、α′缺失)大豆为原料提取大豆分离蛋白(Soy Protein Isolate,SPI),通过十二烷基磺酸钠聚丙烯酰胺凝胶电泳(Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis,SDS-PAGE)技术分析其亚基组成,然后制备乳状液并测定乳化活性指数(Emulsifying Activity Index,EAI)、ζ-电位、粒径、微观结构、稳定动力学指数(Turbiscan Stability Index,TSI)及界面蛋白吸附量。结果表明:α′亚基缺失型SPI乳状液乳化活性指数最大,为87.59 m^2/g;ζ-电位绝对值最大,为47.7 mV;粒径最小,为2.223μm;显微结构显示其分子最小且分布最均匀,稳定动力学指数最小;界面蛋白吸附量最大,为31.40%。4种不同SPI乳状液的稳定性结果由大到小为α′亚基缺失型、东农47、α亚基缺失型、α和α′亚基缺失型。研究结果可为高乳化性大豆蛋白系列产品的开发应用提供理论支撑和技术支持。展开更多
基金Supported by China's National Key Technology R&D Programs (2007BAD88B01-05 2007BAD89B05)
文摘Nitrogen（N） is considered as an important yield and quality-determining factor in soybean production.The objective of the current experiment was to determine the regularity of N accumulation and its simulation among different varieties.This work was conducted with frame tests（pot without bottom）,and three cultivars,HH41,SN14,HN40,and one feed soybean variety MSD was used as materials.The results showed that the regularity of N accumulation in four varieties with the same tendency increased during the whole growth period,which was up to the maximum when the plant was harvested.The longer growth period was,the higher N accumulation of cultivars was,HN40〉SN14〉HH41,N accumulation of MSD was the highest because of the property for feed soybean.Regularity of N accumulation in plants could be accurately described by the Logistic equation.During the growth there was a period when N accumulation in the whole plant occurred at an intense rate.The time of onset and its duration varied with different varieties.The maximum daily N accumulation was on seed filling stage,pod bearing stage,seed filling stage and pod bearing stage in HH41,SN14,HN40 and MSD,respectively.
基金supported by the National Key Research and Development Program of China (2016YFD0100201-14)National Natural Science Foundation of China (31401401)Youth Fund of Heilongjiang Academy of Agricultural Sciences (2017XQ04)
文摘The β subunit of soybean [Glycine max (L.) Merr.] seed storage protein is of great significance in sulfur-containing amino acid balance and soybean processing properties. The objective of this study was to elucidate the relationship between the β subunit and sulfur-containing amino acid composition, and the potential regulatory mechanism. The β subunit was independently accumulated in comparison with other major subunits (α/α′, acidic, basic, and A3) during seed filling, and a low level of β subunit content (BSC) was formed during the accumulation process. In low-BSC mature seeds, crude protein, oil content, and fatty acid composition were not changed, but sulfur-containing amino acids (Cys + Met) in the low- BSC seeds increased significantly (by 31.5%), suggesting that an internal regulatory mechanism within seed might be responsible for the rebalance of seed protein composition and that sulfur assimilation might be deeply involved in β subunit accumulation. Transcriptomic analysis revealed that genes involved in anabolism of cysteine, methionine, and glutathione were up-regulated but those involved in the catabolism of these compounds were down-regulated, suggesting a relationship between the elevation of methionine and glutathione and low BSC. Our study sheds light on seed composition in low BSC lines and on the potential molecular regulatory mechanism of β subunit accumulation, broadening our understanding of soybean seed protein synthesis and its regulation.
