Random amplified polymorphic DNA (RAPD) markers were used to study the DNA polymorphism in Indian mungbean cultivars. A total of 60 random primers were used in the study and 33 of them generated reproducible RAPD patt...Random amplified polymorphic DNA (RAPD) markers were used to study the DNA polymorphism in Indian mungbean cultivars. A total of 60 random primers were used in the study and 33 of them generated reproducible RAPD patterns. Amplification of genomic DNA of most popular 24 Indian mungbean cultivars with these RAPD primers yielded 249 fragments that could be scored, of which 224 were polymorphic, with an average of 7.0 polymorphic fragments per primer. Number of amplified fragments with random primers ranged from 2 (OPI 9) to 17 (OPD 7). Percentage polymorphism ranged from 33% (OPX 5) to a maximum of 100% (OPX 4, OPX 6, OPX 13, OPX 15, OPX 19, OPD 5, OPD 7, OPD 20, OPI 4, OPI 6, OPI 13, OPI 14, OPI 18 and OPF 1), with an average of 90%. The Jaccard’s similarity indices based on RAPD profiles were subjected to UPGMA cluster analysis. And genotypes grouped in two major groups. Sixteen out of 24 released cultivars grouped to cluster I. This indicated the narrow genetic base in the Indian mungbean cultivars used in the study. The details of diversity analysis and possible reasons for narrow genetic base in mungbean cultivars are discussed in the present study.展开更多
<strong>Introduction:</strong><span style="font-family:""><span style="font-family:Verdana;"> Malaria management has been a source of concern for health systems since t...<strong>Introduction:</strong><span style="font-family:""><span style="font-family:Verdana;"> Malaria management has been a source of concern for health systems since the advent of the Covid-19 pandemic. </span><b><span style="font-family:Verdana;">Objective:</span></b><span style="font-family:Verdana;"> To assess the impact of the Covid-19 pandemic on severe childhood malaria in Brazzaville. </span><b><span style="font-family:Verdana;">Material and Method:</span></b><span style="font-family:Verdana;"> A quasi-experimental intervention/non-intervention study was carried out between March and October 2020 in the pediatric departments of the Brazzaville University Hospital. Children aged three months to 15 years hospitalized were the target population. Two groups were formed: the </span></span><span style="font-family:Verdana;">“</span><span style="font-family:Verdana;">intervention</span><span style="font-family:Verdana;">”</span><span style="font-family:Verdana;"> group, that of children hospitalized between March and October 2020 and the </span><span style="font-family:Verdana;">“</span><span style="font-family:Verdana;">control</span><span style="font-family:Verdana;">”</span><span style="font-family:""><span style="font-family:Verdana;"> group that of those hospitalized between January and August 2015. The study variables were epidemiological, clinical, biological and therapeutic. Chi-square and T-Student tests were used. The impact of the intervention was assessed by the absolute risk difference. </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> Of 1392 children hospitalized, 199 (14.6%) had severe malaria with an average age of 6.94 years. These were children under 5 years old n = 95 (47.7%) of low socioeconomic level n = 145 (72.9%) seen on average after 4.6 +/</span></span><span style="font-family:""><span style="font-family:Verdana;">?</span><span><span style="font-family:Verdana;"> 2.4 days. Repeated convulsions (56.8%) and anemia (20.1%) were the main reasons for hospitalization. These were isolated forms (n = 146;73.4%) of which n = 84 (42.2%) neurological and n = 62 (31.2%) anemic. The lethality was 13.1%. Delayed consultation, fever, repeated convulsions, pallor, respiratory distress, sickle cell anemia, thrombocytopenia and hypoglycemia are associated with death. The risk difference for signs of severity between the two periods was 16.6 for repeated convulsions;14.3 for severe anemia. The relative risk between the two studies was 1.8. </span><b><span style="font-family:Verdana;">Conclusion: </span></b><span style="font-family:Verdana;">The increase in morbidity and mortality in severe malaria since the beginning of the Covid-19 pandemic encourages the maintenance of the balance between the management of the Covid-19 pandemic and that of other worrying health problems.</span></span></span>展开更多
<strong>Introduction:</strong><span style="font-family:Verdana;"> Adolescent morbidity and mortality is a health concern.</span><span style="font-family:""> </...<strong>Introduction:</strong><span style="font-family:Verdana;"> Adolescent morbidity and mortality is a health concern.</span><span style="font-family:""> </span><b><span style="font-family:Verdana;">Objective:</span></b><span style="font-family:Verdana;"> To determine morbidity, identify the causes of morbi-mortality and the factors associated with adolescent mortality in Brazzaville</span><span style="font-family:Verdana;">. </span><b><span style="font-family:Verdana;">Patients and Methods:</span></b><span style="font-family:Verdana;"> A retrospective, descriptive and analytical study was carried out at the Brazzaville Hospital and University Center between January 2015 and December 2016. Adolescents hospitalized in clinical services whose records were workable were included. The Epidemiological, clinical and evolutionary variables were analyzed. The statistical test used was the khi-two, the significance level set at 0.05.</span><span style="font-family:""> </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> In total, Out of 67</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;">488 hospitalized patients, 2062 were adolescents: 3% including 891 (43.2%) boys and 1171 (56.8%) girls mean age 14.8 ± 2.92 years, seen in average 9.38 days after the onset of symptoms. Childbirth 415 (20.1%), malaria 309 cases (14.9%), major sickle cell crisis 223 (10.8%) and traumatic injuries 166 cases (8.05%) are the main causes of morbidity. One death was recorded in n</span><span style="font-family:""> </span><span style="font-family:Verdana;">= 193 (9.4%), of which n</span><span style="font-family:""> </span><span style="font-family:Verdana;">=</span><span style="font-family:""> </span><span style="font-family:Verdana;">104 (5.0%)</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">in the first 48. Tumors, malaria and status epilepticus are the leading causes of death. The female sex, the age between 15 and 19 years, the young age of the father, the higher level of education of the adolescent and the low socio-economic level of the parents are associated with the death. </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> Adolescent morbidity is worrying and mortality is significant. It is important that policies on this issue be developed with an emphasis on the main causes of morbi-mortality, including malaria control and sex education.</span></span>展开更多
In this article we analyse 3D models of cultural heritage with the aim of answering three main questions:what processes can be put in place to create a FAIR-by-design digital twin of a temporary exhibition?What are th...In this article we analyse 3D models of cultural heritage with the aim of answering three main questions:what processes can be put in place to create a FAIR-by-design digital twin of a temporary exhibition?What are the main challenges in applying FAIR principles to 3D data in cultural heritage studies and how are they different from other types of data(e.g.images)from a data management perspective?We begin with a comprehensive literature review touching on:FAIR principles applied to cultural heritage data;representation models;both Object Provenance Information(OPI)and Metadata Record Provenance Information(MRPI),respectively meant as,on the one hand,the detailed history and origin of an object,and-on the other hand-the detailed history and origin of the metadata itself,which describes the primary object(whether physical or digital);3D models as cultural heritage research data and their creation,selection,publication,archival and preservation.We then describe the process of creating the Aldrovandi Digital Twin,by collecting,storing and modelling data about cultural heritage objects and processes.We detail the many steps from the acquisition of the Digital Cultural Heritage Objects(DCHO),through to the upload of the optimised DCHO onto a web-based framework(ATON),with a focus on open technologies and standards for interoperability and preservation.Using the FAIR Principles for Heritage Library,Archive and Museum Collections[1]as a framework,we look in detail at how the Digital Twin implements FAIR principles at the object and metadata level.We then describe the main challenges we encountered and we summarise what seem to be the peculiarities of 3D cultural heritage data and the possible directions for further research in this field.展开更多
文摘Random amplified polymorphic DNA (RAPD) markers were used to study the DNA polymorphism in Indian mungbean cultivars. A total of 60 random primers were used in the study and 33 of them generated reproducible RAPD patterns. Amplification of genomic DNA of most popular 24 Indian mungbean cultivars with these RAPD primers yielded 249 fragments that could be scored, of which 224 were polymorphic, with an average of 7.0 polymorphic fragments per primer. Number of amplified fragments with random primers ranged from 2 (OPI 9) to 17 (OPD 7). Percentage polymorphism ranged from 33% (OPX 5) to a maximum of 100% (OPX 4, OPX 6, OPX 13, OPX 15, OPX 19, OPD 5, OPD 7, OPD 20, OPI 4, OPI 6, OPI 13, OPI 14, OPI 18 and OPF 1), with an average of 90%. The Jaccard’s similarity indices based on RAPD profiles were subjected to UPGMA cluster analysis. And genotypes grouped in two major groups. Sixteen out of 24 released cultivars grouped to cluster I. This indicated the narrow genetic base in the Indian mungbean cultivars used in the study. The details of diversity analysis and possible reasons for narrow genetic base in mungbean cultivars are discussed in the present study.
文摘<strong>Introduction:</strong><span style="font-family:""><span style="font-family:Verdana;"> Malaria management has been a source of concern for health systems since the advent of the Covid-19 pandemic. </span><b><span style="font-family:Verdana;">Objective:</span></b><span style="font-family:Verdana;"> To assess the impact of the Covid-19 pandemic on severe childhood malaria in Brazzaville. </span><b><span style="font-family:Verdana;">Material and Method:</span></b><span style="font-family:Verdana;"> A quasi-experimental intervention/non-intervention study was carried out between March and October 2020 in the pediatric departments of the Brazzaville University Hospital. Children aged three months to 15 years hospitalized were the target population. Two groups were formed: the </span></span><span style="font-family:Verdana;">“</span><span style="font-family:Verdana;">intervention</span><span style="font-family:Verdana;">”</span><span style="font-family:Verdana;"> group, that of children hospitalized between March and October 2020 and the </span><span style="font-family:Verdana;">“</span><span style="font-family:Verdana;">control</span><span style="font-family:Verdana;">”</span><span style="font-family:""><span style="font-family:Verdana;"> group that of those hospitalized between January and August 2015. The study variables were epidemiological, clinical, biological and therapeutic. Chi-square and T-Student tests were used. The impact of the intervention was assessed by the absolute risk difference. </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> Of 1392 children hospitalized, 199 (14.6%) had severe malaria with an average age of 6.94 years. These were children under 5 years old n = 95 (47.7%) of low socioeconomic level n = 145 (72.9%) seen on average after 4.6 +/</span></span><span style="font-family:""><span style="font-family:Verdana;">?</span><span><span style="font-family:Verdana;"> 2.4 days. Repeated convulsions (56.8%) and anemia (20.1%) were the main reasons for hospitalization. These were isolated forms (n = 146;73.4%) of which n = 84 (42.2%) neurological and n = 62 (31.2%) anemic. The lethality was 13.1%. Delayed consultation, fever, repeated convulsions, pallor, respiratory distress, sickle cell anemia, thrombocytopenia and hypoglycemia are associated with death. The risk difference for signs of severity between the two periods was 16.6 for repeated convulsions;14.3 for severe anemia. The relative risk between the two studies was 1.8. </span><b><span style="font-family:Verdana;">Conclusion: </span></b><span style="font-family:Verdana;">The increase in morbidity and mortality in severe malaria since the beginning of the Covid-19 pandemic encourages the maintenance of the balance between the management of the Covid-19 pandemic and that of other worrying health problems.</span></span></span>
文摘<strong>Introduction:</strong><span style="font-family:Verdana;"> Adolescent morbidity and mortality is a health concern.</span><span style="font-family:""> </span><b><span style="font-family:Verdana;">Objective:</span></b><span style="font-family:Verdana;"> To determine morbidity, identify the causes of morbi-mortality and the factors associated with adolescent mortality in Brazzaville</span><span style="font-family:Verdana;">. </span><b><span style="font-family:Verdana;">Patients and Methods:</span></b><span style="font-family:Verdana;"> A retrospective, descriptive and analytical study was carried out at the Brazzaville Hospital and University Center between January 2015 and December 2016. Adolescents hospitalized in clinical services whose records were workable were included. The Epidemiological, clinical and evolutionary variables were analyzed. The statistical test used was the khi-two, the significance level set at 0.05.</span><span style="font-family:""> </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> In total, Out of 67</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;">488 hospitalized patients, 2062 were adolescents: 3% including 891 (43.2%) boys and 1171 (56.8%) girls mean age 14.8 ± 2.92 years, seen in average 9.38 days after the onset of symptoms. Childbirth 415 (20.1%), malaria 309 cases (14.9%), major sickle cell crisis 223 (10.8%) and traumatic injuries 166 cases (8.05%) are the main causes of morbidity. One death was recorded in n</span><span style="font-family:""> </span><span style="font-family:Verdana;">= 193 (9.4%), of which n</span><span style="font-family:""> </span><span style="font-family:Verdana;">=</span><span style="font-family:""> </span><span style="font-family:Verdana;">104 (5.0%)</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">in the first 48. Tumors, malaria and status epilepticus are the leading causes of death. The female sex, the age between 15 and 19 years, the young age of the father, the higher level of education of the adolescent and the low socio-economic level of the parents are associated with the death. </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> Adolescent morbidity is worrying and mortality is significant. It is important that policies on this issue be developed with an emphasis on the main causes of morbi-mortality, including malaria control and sex education.</span></span>
基金supported by the United States Agency for International Development under the terms of Award No. HRN-a-00-00-00018-00supported by the International Clinical+1 种基金 Operational and Health Services Research Program in TB and AIDS (TW-006900) in pursuance of obtaining a doctoral degree (PhD)
基金partially funded by Project PE 0000020 CHANGES-CUP B53C22003780006NRP Mission 4 Component 2 Investment 1.3,Funded by the European Union-NextGenerationEU
文摘In this article we analyse 3D models of cultural heritage with the aim of answering three main questions:what processes can be put in place to create a FAIR-by-design digital twin of a temporary exhibition?What are the main challenges in applying FAIR principles to 3D data in cultural heritage studies and how are they different from other types of data(e.g.images)from a data management perspective?We begin with a comprehensive literature review touching on:FAIR principles applied to cultural heritage data;representation models;both Object Provenance Information(OPI)and Metadata Record Provenance Information(MRPI),respectively meant as,on the one hand,the detailed history and origin of an object,and-on the other hand-the detailed history and origin of the metadata itself,which describes the primary object(whether physical or digital);3D models as cultural heritage research data and their creation,selection,publication,archival and preservation.We then describe the process of creating the Aldrovandi Digital Twin,by collecting,storing and modelling data about cultural heritage objects and processes.We detail the many steps from the acquisition of the Digital Cultural Heritage Objects(DCHO),through to the upload of the optimised DCHO onto a web-based framework(ATON),with a focus on open technologies and standards for interoperability and preservation.Using the FAIR Principles for Heritage Library,Archive and Museum Collections[1]as a framework,we look in detail at how the Digital Twin implements FAIR principles at the object and metadata level.We then describe the main challenges we encountered and we summarise what seem to be the peculiarities of 3D cultural heritage data and the possible directions for further research in this field.
