Edible bird's nest(EBN),abundant in sialic acid(SA)has been recognized as a natural substance beneficial to brain development and cognitive ability.In order to investigate active component of EBN,sialylated glycop...Edible bird's nest(EBN),abundant in sialic acid(SA)has been recognized as a natural substance beneficial to brain development and cognitive ability.In order to investigate active component of EBN,sialylated glycopeptides(SCP)were extracted from EBN by 60%ethanol after trypsin hydrolysis and its effects on central nervous system was evaluated in lipopolysaccharide(LPS)-induced neuroinflammation mice.EBN,SCP,and SA pretreatment and intervention reduced interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),and interleukin-6(IL-6)levels in hippocampus and cortex,meanwhile inhibited the activation of microglia,astrogliosis and neuron apoptosis,which were consistent with the learning and memory improvement and anti-depression effects in behavioral tests.The counts of leukocytes,neutrophils and monocytes,as well as IL-1β,TNF-α,and IL-6 levels in peripheral circulation were significantly reduced by SCP.Results of plasma metabolomics suggested SCP up-regulated energy metabolism,promoted the recovery of primary and secondary bile acid metabolism and indole metabolism,where microbiota may involve.16S rDNA sequencing of colonic contents showed EBN,SCP and SA repaired dysbacteriosis in LPS-treated mice by significantly up-regulating the anti-inflammatory Muribaculaceae and inhibiting pro-inflammatory related Desulfovibrio and Candidatus_Saccharimonas.In addition,both EBN and SCP could significantly enrich Aerococcus,while SA could specifically enrich Prevotellaceae_UCG_001.The gut-brain axis was preliminarily established,and SCP may have the potential to be a functional factor for neuroprotection applied in EBN industry.展开更多
By means of vertical normal modes a regional nested multilevel primitive equation model can be reduced to several sets of shallow water equations characterized by various equivalent depths. Therefore, time integration...By means of vertical normal modes a regional nested multilevel primitive equation model can be reduced to several sets of shallow water equations characterized by various equivalent depths. Therefore, time integration of the model in spectral form can be performed in the manner similar to those used in the spectral nested shallow water equation model case.展开更多
Edible bird’s nest(EBN)is a kind of natural invigorant with a long history of consumption in Asia,especially in China.EBN is formed by mixing the saliva of swiftlets(Aerodramus)with feathers and other components duri...Edible bird’s nest(EBN)is a kind of natural invigorant with a long history of consumption in Asia,especially in China.EBN is formed by mixing the saliva of swiftlets(Aerodramus)with feathers and other components during the breeding season.Proteins are the most important nutrient in EBN.By studying proteins in EBN,we can not only elucidate their components at the molecular level,but also study their bioactivities.Therefore,it is of great significance to study the proteins in EBN.Previous research on the proteins in EBN was preliminary and cursory,and no one has summarized and analyzed the proteins in EBN and correlated the bioactivities of these proteins with the biological functions of EBN.This article focused on the proteins in EBN,listed the proteins identified in different proteomic studies,and introduced the sources,structures and bioactivities of the most frequently identified proteins,including acidic mammalian chitinase,lysyl oxidase homolog 3,mucin-5AC,ovoinhibitor,nucleobindin-2,calciumbinding protein(MW:4.5×10~4)and glucose-regulated protein(MW:7.8×10~4).The properties of these proteins are closely related to the bioactivities of EBN.Therefore,this article can provide inspiration for further research on the efficacy of EBN.展开更多
A phase singularity of the light field created by interference of two Gaussian singular beams which propagate in a weak and near ground turbulent atmosphere is analyzed by the Rytov approximation and the short-term av...A phase singularity of the light field created by interference of two Gaussian singular beams which propagate in a weak and near ground turbulent atmosphere is analyzed by the Rytov approximation and the short-term averaging method of the dislocation-position. We demonstrate that an edge or circular dislocation may be formed by both parallel and coaxial or noncoaxial collimated beams with different or equal beam-width interference. The edge or circular short-term wavefront dislocation of super position field depend on the atmospheric turbulence strength, beam propagation distance, amplitude ratio, dislocation of nesting vortices, and beam-width or beam-width ratio of the individual beams.展开更多
Photodynamic therapy(PDT)has been established as one of the most promising novel cancer therapies with fewer side-effects and enhanced efficacy compared to the currently available conventional treatments.However,its a...Photodynamic therapy(PDT)has been established as one of the most promising novel cancer therapies with fewer side-effects and enhanced efficacy compared to the currently available conventional treatments.However,its application has been hindered by the limitations that photosensitizers(PS)have.The combination of PS with metallic nanoparticles like platinum nanoparticles(PtNPs),can help to overcome these intrinsic drawbacks.In this work,the combination of PtNPs and the natural photosensitizer riboflavin(RF)is proposed.PtNPs are synthesized using RF(Pt@RF)as reducing and stabilizing agent in a one-step method,obtaining nanoparticles with mesoporous structure for UV triggered PDT.In view of possible future UV irradiation treatments,the degradation products of RF,ribitol(RB)and lumichrome(LC),this last being a photosensitizing byproduct,are also employed for the synthesis of porous PtNPs,obtaining Pt@LC and Pt@RB.When administered in vitro to lung cancer cells,all the samples elicit a strong decrease of cell viability and a decrease of intracellular ATP levels.The antitumoral effect of both Pt@RF and Pt@LC is triggered by UV-A irradiation.This antitumoral activity is caused by the induction of oxidative stress,shown in our study by the decrease in intracellular glutathione and increased expression of antioxidant enzymes.展开更多
基金funded by Central Nervous System Product Research and Development Key Laboratory of Sichuan Province(230048-01SZ).
文摘Edible bird's nest(EBN),abundant in sialic acid(SA)has been recognized as a natural substance beneficial to brain development and cognitive ability.In order to investigate active component of EBN,sialylated glycopeptides(SCP)were extracted from EBN by 60%ethanol after trypsin hydrolysis and its effects on central nervous system was evaluated in lipopolysaccharide(LPS)-induced neuroinflammation mice.EBN,SCP,and SA pretreatment and intervention reduced interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),and interleukin-6(IL-6)levels in hippocampus and cortex,meanwhile inhibited the activation of microglia,astrogliosis and neuron apoptosis,which were consistent with the learning and memory improvement and anti-depression effects in behavioral tests.The counts of leukocytes,neutrophils and monocytes,as well as IL-1β,TNF-α,and IL-6 levels in peripheral circulation were significantly reduced by SCP.Results of plasma metabolomics suggested SCP up-regulated energy metabolism,promoted the recovery of primary and secondary bile acid metabolism and indole metabolism,where microbiota may involve.16S rDNA sequencing of colonic contents showed EBN,SCP and SA repaired dysbacteriosis in LPS-treated mice by significantly up-regulating the anti-inflammatory Muribaculaceae and inhibiting pro-inflammatory related Desulfovibrio and Candidatus_Saccharimonas.In addition,both EBN and SCP could significantly enrich Aerococcus,while SA could specifically enrich Prevotellaceae_UCG_001.The gut-brain axis was preliminarily established,and SCP may have the potential to be a functional factor for neuroprotection applied in EBN industry.
文摘By means of vertical normal modes a regional nested multilevel primitive equation model can be reduced to several sets of shallow water equations characterized by various equivalent depths. Therefore, time integration of the model in spectral form can be performed in the manner similar to those used in the spectral nested shallow water equation model case.
基金financially supported by the Fundamental Research Funds for the Central Universities(lzujbky-2022-ct03)。
文摘Edible bird’s nest(EBN)is a kind of natural invigorant with a long history of consumption in Asia,especially in China.EBN is formed by mixing the saliva of swiftlets(Aerodramus)with feathers and other components during the breeding season.Proteins are the most important nutrient in EBN.By studying proteins in EBN,we can not only elucidate their components at the molecular level,but also study their bioactivities.Therefore,it is of great significance to study the proteins in EBN.Previous research on the proteins in EBN was preliminary and cursory,and no one has summarized and analyzed the proteins in EBN and correlated the bioactivities of these proteins with the biological functions of EBN.This article focused on the proteins in EBN,listed the proteins identified in different proteomic studies,and introduced the sources,structures and bioactivities of the most frequently identified proteins,including acidic mammalian chitinase,lysyl oxidase homolog 3,mucin-5AC,ovoinhibitor,nucleobindin-2,calciumbinding protein(MW:4.5×10~4)and glucose-regulated protein(MW:7.8×10~4).The properties of these proteins are closely related to the bioactivities of EBN.Therefore,this article can provide inspiration for further research on the efficacy of EBN.
基金This work was supported by the Key Project of Chinese Ministry of Education (No. 01091)
文摘A phase singularity of the light field created by interference of two Gaussian singular beams which propagate in a weak and near ground turbulent atmosphere is analyzed by the Rytov approximation and the short-term averaging method of the dislocation-position. We demonstrate that an edge or circular dislocation may be formed by both parallel and coaxial or noncoaxial collimated beams with different or equal beam-width interference. The edge or circular short-term wavefront dislocation of super position field depend on the atmospheric turbulence strength, beam propagation distance, amplitude ratio, dislocation of nesting vortices, and beam-width or beam-width ratio of the individual beams.
基金funded by the Horizon Europe Project"PERSEUS"(No.101099423)financed by the Ministry of Universities under application 33.50.460A.752by the European Union NextGenerationEU/PRTR through a contract Margarita Salas from Universidade de Vigo.
文摘Photodynamic therapy(PDT)has been established as one of the most promising novel cancer therapies with fewer side-effects and enhanced efficacy compared to the currently available conventional treatments.However,its application has been hindered by the limitations that photosensitizers(PS)have.The combination of PS with metallic nanoparticles like platinum nanoparticles(PtNPs),can help to overcome these intrinsic drawbacks.In this work,the combination of PtNPs and the natural photosensitizer riboflavin(RF)is proposed.PtNPs are synthesized using RF(Pt@RF)as reducing and stabilizing agent in a one-step method,obtaining nanoparticles with mesoporous structure for UV triggered PDT.In view of possible future UV irradiation treatments,the degradation products of RF,ribitol(RB)and lumichrome(LC),this last being a photosensitizing byproduct,are also employed for the synthesis of porous PtNPs,obtaining Pt@LC and Pt@RB.When administered in vitro to lung cancer cells,all the samples elicit a strong decrease of cell viability and a decrease of intracellular ATP levels.The antitumoral effect of both Pt@RF and Pt@LC is triggered by UV-A irradiation.This antitumoral activity is caused by the induction of oxidative stress,shown in our study by the decrease in intracellular glutathione and increased expression of antioxidant enzymes.
