Fall Armyworms (FAW) Spodoptera frugiperda, particularly in maize crops, is a major agricultural pest. Current control methods rely heavily on chemical pesticides, which are costly and harmful to non-target organisms....Fall Armyworms (FAW) Spodoptera frugiperda, particularly in maize crops, is a major agricultural pest. Current control methods rely heavily on chemical pesticides, which are costly and harmful to non-target organisms. To provide a sustainable alternative, the research isolated indigenous Entomopathogenic fungi, Metarhizium sp. and Trichoderma sp., from FAW-infested areas. The fungi were tested for virulence against FAW and a non-target insect, Cirina butyrospermi. Results showed that Metarhizium sp. FAW was highly effective in killing FAW while sparing Cirina butyrospermi, demonstrating its potential as a biological control agent. In addition, FAW infected with Metarhizium sp. exhibited a significant reduction in food consumption. The study highlights the potential use of indigenous fungi in integrated pest management systems, reducing the reliance on chemical pesticides and supporting sustainable agriculture. Further research is needed to understand the interaction of these promising Metarhizum strains with soil microbiota and their long-term efficacy in field conditions.展开更多
Background: The Coronavirus disease 2019 (COVID-19) pandemic, caused by the SARS-CoV-2 coronavirus, remains a global threat despite lifting the health emergency. Scientists from all continents have been mobilized to d...Background: The Coronavirus disease 2019 (COVID-19) pandemic, caused by the SARS-CoV-2 coronavirus, remains a global threat despite lifting the health emergency. Scientists from all continents have been mobilized to develop vaccines and medicines for prevention and cure. In Burkina Faso, traditional healers proposed using Scoparia dulcis L., a medicinal plant, to manage COVID-19. Method: In silico screening offers a quick drug-likeness evaluation of Scoparia dulcis L.-isolated biomolecules toward SARS-CoV-2 targets, such as Mpro protease. A review of the literature retrieved 35 biomolecules isolated from Scoparia dulcis. The potential interactions of these biomolecules with the amino acid residues of the SARS-CoV-2 Mpro protease were visualized. Affinities and probable oral route delivery were assessed using reference molecules such as remdesivir and nelfinavir. Results: The screening allowed the retention of 20 hit molecules, which had a better affinity for the target than the reference molecules remdesivir and nelfinavir, and analysis of the results identified height lead molecules with a significant interaction with the Mpro protease and being druggable. There are six flavonoids: cirsimarin, cynaroside, hydroxy-tetramethoxyflavone, gossypetin, luteolin, vitexin, one diterpene, glutinol, and one glycoside, eugenyl-glucoside. These molecules interact with methionine 6 and tyrosine 126 of SARS-CoV-2 Mpro. These two amino acids are essential for the dimerization of Mpro protease. Inhibitory action on Mpro protease can be expected from these biomolecules. Conclusion: Scoparia dulcis L. could help manage COVID-19 because it contains biomolecules that can inactivate SARS-CoV-2 Mpro.展开更多
One of the most promising vector control strategies for controlling dengue fever is the stable introduction of the obligate intracellular Wolbachia bacteria strain into Aedes aegypti mosquitoes. This method reduces th...One of the most promising vector control strategies for controlling dengue fever is the stable introduction of the obligate intracellular Wolbachia bacteria strain into Aedes aegypti mosquitoes. This method reduces the mosquito’s ability to transmit dengue through reproductive strategies associated with Wolbachia infection, such as parthenogenesis, male elimination or feminization, sex ratio distortions and cytoplasmic incompatibility. Expert knowledge and a risk assessment framework were used to identify the risks associated with the release of Wolbachia-bearing mosquitoes. Then, consultations with individual participants were organized and a Bayesian network (BN) was developed to capture the relationship between the hazards as well as the likelihood of these adverse events occurring. Finally, risk was calculated from the probability and consequence estimates obtained from our Burkina Faso participants, using a questionnaire based on the identified risks. Our “Cause More Harm” study yielded 46.15% negligible risk, 44.23% very low risk and 9.62% low risk. The “socio-cultural change” parameter had the greatest influence, with the perception that the dissemination project would be poorly received by the local population. This parameter alone accounted for 80% of the most significant risks. This explains the fact that hazard RA 49 “negative messages in social media” is ranked as the highest individual risk (although the risk is low) with a calculated risk of 0.261. The risk assessment was designed to integrate the interdependent complexity of hazards likely to affect the dissemination of technology in the environment. It represents an important implementation phase in the success of this innovative research, introducing a new technology to combat the transmission of dengue fever.展开更多
Over the last few decades, dengue fever epidemics have increased in frequency and intensity worldwide, making it a major global concern for public health. Its prevention, which is essentially vector-based control, is ...Over the last few decades, dengue fever epidemics have increased in frequency and intensity worldwide, making it a major global concern for public health. Its prevention, which is essentially vector-based control, is already being compromised by reports of resistance of the main vector Aedes aegypti to insecticides. To tackle the rapid increase in insecticide resistance and outbreaks, the biological vector control is a promising approach. One of the strategies of this approach is the use of entomopathogenic fungi because of their great efficacy and their eco-friendly aspects. However, some aspects of their use, such as the low efficiency, the high cost of production and the sensitivity to various adverse conditions, need to be addressed for their successful large-scale application. Therefore, innovative technologies based on strains of transgenic fungi with improved biocontrol potentials by genetic engineering are actively pursued. Although these modified mycoinsecticides are acclaimed for their better effectiveness against target insects, the main concern remains their potential adverse effects on the environment and human health. The present review is dedicated to giving an update on recent developments in transgenic entomopathogenic fungi (TEF) for Aedes mosquito control. Future perspectives are also proposed to address the safety concerns related to the release of transgenic entomopathogenic fungi into the environment.展开更多
In Burkina Faso, recent dengue outbreaks were exacerbated by Aedes aegypti mosquitoes, which have developed resistance to conventional insecticides. This study investigates the potential of Metarhizium pingshaense Met...In Burkina Faso, recent dengue outbreaks were exacerbated by Aedes aegypti mosquitoes, which have developed resistance to conventional insecticides. This study investigates the potential of Metarhizium pingshaense Met_S26, a fungus from Burkina Faso, for controlling both endophilic and exophilic Aedes mosquitoes. Traditional interventions are less effective against mosquitoes resting outdoors, and the ability of the fungus to be sexually transmitted could enhance its efficacy. Aedes mosquitoes were reared and exposed to various concentrations of Metarhizium pingshaense to assess its virulence. The fungus showed significant efficacy, with lethal times (LT80) ranging from 8.67 to 11.83 days depending on the concentration. Furthermore, bioassays demonstrated effective sexual auto-dissemination, with substantial transmission of fungal spores between infected and uninfected mosquitoes, leading to lower survival rates in uninfected mates. This study highlights Metarhizium pingshaense Met_S26 as a promising tool for controlling both indoor and outdoor Aedes populations, complementing existing vector control strategies such as the Sterile Insect Technique (SIT) and Wolbachia-based approaches. Future research should explore integrating this fungus with other control methods for a comprehensive vector management strategy.展开更多
Malaria is a potentially lethal disease caused by parasites of the Plasmodium genus, transmitted to humans through the bite of infected female mosquitoes, primarily Anopheles. To control this disease, various strategi...Malaria is a potentially lethal disease caused by parasites of the Plasmodium genus, transmitted to humans through the bite of infected female mosquitoes, primarily Anopheles. To control this disease, various strategies are implemented, including biological control, which targets the vectors of the parasite. This approach uses biological agents such as entomopathogenic fungi, including Metarhizium pingshaense, a fungus capable of causing lethal infections in mosquitoes. The production of Metarhizium pingshaense is still limited in Burkina Faso, and local cultivation of this fungus could help fill this gap. A study was conducted to identify optimal local substrates that promote its growth. Indeed, after gathering information on the dietary habits of populations in Bobo-Dioulasso and Bama, three potential substrates were selected: rice, cornmeal dough (MFL), and beans. These substrates were inoculated with two strains of Metarhizium pingshaense (S10 and S26) to assess their ability to support fungal growth and their effectiveness. Experimental results showed that MFL and bean substrates favored optimal growth of Metarhizium pingshaense, with growths of 1.91 cm and 2.13 cm after 8 days, compared to 1.83 cm on a standard media (PDA). In terms of virulence, S26 strain caused 60% mosquito mortality on both the bean and PDA media, while S10 strain induced mortalities of 50% for bean and 62% for PDA.展开更多
Introduction: A recent study in Kenya highlighted a promising advance in malaria control by demonstrating that infecting mosquitoes with the endosymbiont Microsporidia MB blocks Plasmodium transmission. However, the i...Introduction: A recent study in Kenya highlighted a promising advance in malaria control by demonstrating that infecting mosquitoes with the endosymbiont Microsporidia MB blocks Plasmodium transmission. However, the influence of biotic and abiotic factors such as diet, relative humidity (RH) and temperature on this infection remains poorly studied. This study, aimed to gain a better understanding of this relationship. Methods: To highlight the influence of diet quantity, we defined a range of 3 quantities: 0.00375 g, 0.015 g and 0.09 g. Each quantity was tested on two groups of larvae: a group of 150 larvae infected with Microsporidia MB (MB+), and a group of 150 larvae not infected with Microsporidia MB (MB−) (control group), each divided into three replicates of 50 larvae. Each replicate was fed each morning with the assigned quantity until the pupal stage. In addition to this factor, we investigated the influence of temperature and RH. We defined three temperature-RH combinations: 21˚C-80% RH, 39˚C-50% RH, and 27˚C-75% RH. Each combination was tested on two groups of larvae: a group of 150 MB+ larvae and a group of 150 MB− larvae, each divided into three replicates of 50 larvae. Each replicate was subjected to the assigned combination until pupation. Pupae that had reached the adult stage were tested by PCR to determine their Microsporidia MB infection status for each factor studied. Results: The results showed that only the lowest quantity (0.00375 g) significantly reduced the prevalence of Microsporidia MB compared with the medium quantity (chi-2 test, χ2 = 4.9088, df = 1, p = 0.02672) and the high quantity (chi-2 test, χ2 = 4.7958, df = 1, p = 0.02853). As for temperature and RH, the combination 39˚C-50% RH led to a significant reduction in the prevalence of Microsporidia MB compared with the combination 27˚C-75% RH (chi-2 test, χ2 = 6.3736, ddl = 1, p = 0.01158) and that 21˚C-80% RH (chi-2 test, χ2 = 9.983, ddl = 1, p = 0.00158). Conclusion: This work contributes to a better understanding of some key factors linked to Microsporidia MB infection in mosquitoes. However, further research on several generations is necessary to draw more comprehensive conclusions.展开更多
Cervical cancer is the fourth most common cancer worldwide, accounting for 6.8% of new cancer cases and 8.1% of cancer-related deaths. About 85% of these deaths occurred in low- and middle-income countries. The aim of...Cervical cancer is the fourth most common cancer worldwide, accounting for 6.8% of new cancer cases and 8.1% of cancer-related deaths. About 85% of these deaths occurred in low- and middle-income countries. The aim of this study was to assess the frequency and distribution of the human papillomavirus (HPV) genotypes in women showing cytological abnormalities of the cervix at the Sourô SANOU University Hospital (CHUSS) in Bobo-Dioulasso, Burkina Faso. This is a descriptive study of women recruited at the CHUSS. The cervico-uterine smear examination was carried out at the CHUSS Anatomy and Pathology Department for cervical cancer screening. The data were collected from women with atypical cells on their cervico-uterine smear. Cervicovaginal samples were taken from consenting women and HPV genotyping was performed using the HPV Direct FLOW CHIP kit at CERBA. We obtained approval from the ethics committee. The data were analyzed using the SPSS 26 software. The results of the study showed that 67.79% of the participants were aged between 50 and 65, a group that is particularly vulnerable to persistent infection with high-risk oncogenic HPV genotypes. Of the women screened, 40.7% were HPV positive and 29.2% had multiple infections. The most common genotypes were HPV 35, followed by HPV 18, 52, 58 and 66. These data highlight the need for increased surveillance and targeted prevention strategies among this female population.展开更多
Background: In recent years, Burkina Faso has faced increasing insecurity and terrorist attacks. The police, gendarmerie, and justice services are very often in demand to carry out criminal investigations, which are s...Background: In recent years, Burkina Faso has faced increasing insecurity and terrorist attacks. The police, gendarmerie, and justice services are very often in demand to carry out criminal investigations, which are slowed down or incomplete due to the absence of DNA analysis on biological samples from crime scenes and on apprehended suspects. The purpose of this study was to evaluate the contribution of DNA analysis to the resolution of criminal cases in Burkina Faso. Methodology: This study was carried out from June 2019 to July 2020. Three (03) crimes were investigated, and DNA analysis was performed on biological samples from the crime scene, suspects, and victims using the AmpFlSTR® identifiler® Direct kit on the ABI 3130 Genetic Analyzer. Results: In the explosion case, the alleles found in the victim were the same as those identified in the blood trace from the crime scene. In the armed robbery case, there was a perfect match between the DNA profile of the blood trail and that of suspect 2 for all 15 STRs analyzed. In the murder case, the DNA profile of the murdered man’s son and the DNA profile of the biological trace were identical. Conclusion: The DNA analyses carried out in criminal cases have identified the perpetrators of the crimes. Their guilt or innocence will be confirmed by the investigators during the interrogations and hearings.展开更多
Introduction: Human pegivirus (HPgV), initially identified as hepatitis G virus in the 1990s, predominantly causes acute hepatitis and may persist particularly in individuals with compromised immune systems or those c...Introduction: Human pegivirus (HPgV), initially identified as hepatitis G virus in the 1990s, predominantly causes acute hepatitis and may persist particularly in individuals with compromised immune systems or those co-infected with HIV, HBV, or HCV. Despite its potential public health implications, particularly in transfusion contexts, comprehensive epidemiological data on HPgV in Burkina Faso remains scarce. Objectives: This study aimed to determine 1) the prevalence of human pegivirus infection among blood donors at the Regional Blood Transfusion Centre (Koudougou, Burkina Faso), and 2) the rates of co-infection between human pegivirus with HIV, HBV, HCV and Treponema pallidum. Material and Methods: Between 9 and 27 August 2022, 100 blood samples were collected and analyzed at the Regional Blood Transfusion Centre. Screening for HIV, HBV, HCV, and Treponema pallidum was conducted using the Cobas e 601 system (Roche Diagnostics). A 100 μL volume of each donor’s plasma was utilized for viral RNA extraction with the DNA/RNA Prep Kit (Sacace Biotechnologies) following the manufacturer’s instructions. HPgV RNA detection was conducted using the HGV Real-TM amplification kit (Sacace Biotechnologies). Results: The study was comprised of 100 blood donors, identifying HPgV RNA in 14 individuals (14% prevalence), with one noted co-infection with HBV. None of the participants were HIV positive. The prevalence rates for HBV and HCV were each found to be 5%, and syphilis also presented a prevalence of 5%. Conclusion: Our findings indicate a significant prevalence of HPgV among blood donors in Burkina Faso, underscoring the need for heightened surveillance and preventive measures in blood transfusion services and the broader population to enhance transfusion safety and public health.展开更多
DNA testing for biological relationships is becoming increasingly common in Burkina Faso. STR analysis remains the most reliable technique for resolving disputes or claims in court regarding biological relationships. ...DNA testing for biological relationships is becoming increasingly common in Burkina Faso. STR analysis remains the most reliable technique for resolving disputes or claims in court regarding biological relationships. This study aimed to establish the links of biological relationships between subjects by analyzing 21 STR loci. The participants were 14 individuals referred to CERBA by the Justice in 2022. Oral or blood samples were taken for each subject. DNA was extracted, and the analysis of DNA polymorphism by PowerPlex® 21 Kit (Part No. DC8902) was performed by capillary electrophoresis on the ABI PRISM 3130 sequencer. DNA profiles were compiled using the GeneMapper IDX software version 1.2. Of the fourteen subjects examined, twelve of these samples had complete genetic profiles, while the other two had partial and absent profiles. The results confirmed the inclusion of three cases of Paternity, one case of maternity, one case of a relationship of brotherhood, and the exclusion of one case of maternity and one case of a relationship of brotherhood. DNA tests improve the resolution of filiations, but they require ethical and cultural awareness and a strengthened legal framework to prevent and protect society.展开更多
Background:Evidence regarding the effectiveness of prenatal nutritional supplements has mainly considered anthropometric pregnancy outcomes.The effect on markers of health and disease,such as offspring telomere length...Background:Evidence regarding the effectiveness of prenatal nutritional supplements has mainly considered anthropometric pregnancy outcomes.The effect on markers of health and disease,such as offspring telomere length(TL)and mitochondrial DNA content(mtDNAc)is unknown.Objectives:We assessed the efficacy of maternal multiple micronutrient(MMN)-fortified balanced-energy protein(BEP)and iron-folic acid(IFA)supplementation on newborn TL as a secondary outcome and mtDNAc as a non-declared outcome.Design:We conducted a randomized controlled trial in rural Burkina Faso,among pregnant females(15-40 years old)enrolled at<21 weeks of gestation.Mothers received either MMN-fortified BEP and IFA(intervention)or IFA only(control)throughout pregnancy.Whole arterial blood samples were collected from the umbilical cord of 104 control and 90 intervention group infants,respectively.Average relative TL and mtDNAc were measured using quantitative polymerase chain reaction.Linear regression models were fitted to assess TL and mtDNAc differences across trial arms.Results:We found that a combined daily MMN-fortified BEP supplement and IFA tablet did not affect newborn TL[β=-0.010(95%CI:-0.057,0.036);P=0.662]or mtDNAc[β=0.065(95%CI:-0.203,0.073);P=0.354],as compared to an IFA tablet alone.These findings were confirmed(P>0.05)by adjusting the regression models for potential prognostic factors of study outcomes at enrollment.Exploratory analyses indicated higher,but non-significantly different mtDNAc among children born either small-for-gestational age,low birthweight,or preterm.Conclusion:Newborns from mothers who received daily nutritional supplements across gestation did not have different relative TL or mtDNAc.展开更多
Objective:To evaluate the anthelmintic property of Saba senegalensis(A.DC)Pichon(Apocynaceae)(S.senegalensis)on Haemonchus contortus that is traditionally used in Burkina Faso for its gastrointestinal parasites treatm...Objective:To evaluate the anthelmintic property of Saba senegalensis(A.DC)Pichon(Apocynaceae)(S.senegalensis)on Haemonchus contortus that is traditionally used in Burkina Faso for its gastrointestinal parasites treatment.Methods:The lyophilized aqueous decoction of leaves of S.senegalensis at concentrations of 0.10,1.00,3.00,10.00 and 15.00 mg/m L was used on eggs and adult worms of Haemonchus contortus collected from gastrointestinal tract of small ruminant.Results:The LC_(50)on adult worms was 6.79 mg/m L and 3.25 mg/m L for the leaves of S.senegalensis and the levamisole(reference drug),respectively.Inhibition of hatching assay showed a concentration-dependent manner with an inhibition of 93.63%at the concentration of 15.00 mg/m L of S.senegalensis.Conclusions:These results indicate that the aqueous extract of S.senegalensis possesses an anthelmintic property and may justify its use in traditional medicine for the treatment of gastrointestinal parasites.展开更多
Background: Anastatica hierochuntica L. (Brassicaceae) has been claimed to treat various conditions, including complicated childbirth. The principal objective of this research was to provide scientific evidence on the...Background: Anastatica hierochuntica L. (Brassicaceae) has been claimed to treat various conditions, including complicated childbirth. The principal objective of this research was to provide scientific evidence on the contractile effect of the aqueous and hydroethanolic extracts of the whole plant from A. hierochuntica during labor. Method: Qualitative and quantitative methods helped identify and quantify the phytoconstituents in both extracts. The antioxidant activity was studied using four standard methods (ABTS, DPPH, FRAP, and LPO). Results: The inhibitory power of the extracts on pro-inflammatory enzymes such as 15-lipoxygenase and phospholipase A<sub>2</sub> was determined. The contractile effects of the extracts were evaluated using isolated pregnant mice uteri. Steroids, triterpenoids, flavonoids, tannins, coumarins, and reduced compounds were identified in both extracts. The hydroethanolic extract exhibited higher content of hydrolyzable tannins (15.07 ± 0.24 mg tannic acid equivalent/g) than the aqueous decoction (11.8 ± 0.69 mg TAE/g). The extracts demonstrated the ability to scavenge DPPH and ABTS radicals, reduce ferric ions, and inhibit especially lipid peroxidation. No significant difference was noted in the phospholipase inhibition between the extracts and betamethasone. The hydroethanolic extract displayed the most significant anti-lipoxygenase activity with an IC<sub>50</sub> value of 55.82 ± 1.15 μg/mL. The lethal dose (LD<sub>50</sub>) of extracts was estimated to be superior to 2000 mg/kg body weight (bw). The ability of the samples to produce one cm amplitude of contraction at 50% effective concentrations (EC<sub>50</sub>) was 5.33 × 10<sup>−8</sup> ± 0.32 mg/mL for oxytocin, 1.51 ± 0.08 mg/mL for aqueous extract and 3.57 ± 0.61 mg/mL for hydroethanolic extract. Conclusion: These findings could provide evidence for using Anastatica hierochuntica to facilitate childbirth.展开更多
<strong>Objective:</strong> The aim of this study was to assess the level of erythrocyte membrane cholesterol in sickle cell patients, which is one of the essential parameters of membrane fluidity that con...<strong>Objective:</strong> The aim of this study was to assess the level of erythrocyte membrane cholesterol in sickle cell patients, which is one of the essential parameters of membrane fluidity that contributes to understanding the hemolytic state of the erythrocyte. <strong>Methods: </strong>We worked with blood specimens from 20 controls and 50 sickle cell patients. The blood count and the isoelectric focusing (IEF) were performed on the samples in order to select them. The titration of the erythrocyte membrane cholesterol was made after washing and lysing the erythrocytes with the hemolyzing solution (EDTA, 2 Mercapto-Ethanol, NADP, NaOH). The cholesterol level was assessed by the enzymatic colorimetric method. The results were analyzed by Student’s test. <strong>Results: </strong>We worked with 16 control subjects with a hemoglobin status and a normal hemogram. The evaluation of the erythrocyte membrane cholesterol level of the samples allows us to define the reference interval (<em>α</em> = 0.05) at 17.55 ± 3.83 mg of cholesterol/g of hemoglobin. The erythrocyte membrane cholesterol levels of sickle cell patients found in this study were 11.58 ± 2.98 mg cholesterol/g of hemoglobin. In this study, 38 sickle cell patients (76%) were found with a low erythrocyte membrane cholesterol level compared to the reference interval. Statistical analysis showed that there was a significant difference (<em>α </em>= 5%) between the erythrocyte membrane cholesterol level of normal subjects and sickle cell patients. <strong>Conclusion: </strong>Most of the sickle cells patients had a decreased erythrocyte membrane cholesterol level. This reduces the membrane fluidity making the erythrocyte membrane rigid and more fragile. This may be one of the factors responsible for the lyses of erythrocytes.展开更多
<div style="text-align:justify;"> <span style="font-family:Verdana;"><strong>Introduction: </strong>Cirrhosis represents 27.63% of the hepatobiliary diseases in Burkina Faso...<div style="text-align:justify;"> <span style="font-family:Verdana;"><strong>Introduction: </strong>Cirrhosis represents 27.63% of the hepatobiliary diseases in Burkina Faso. We aimed at studying the therapeutic and evolutionary features of the cirrhosis. <strong>Patients and methods: </strong>We implemented a cross-sectional and descriptive study, collecting retrospectively the data from 1st January 2012 to 31st March 2014. The diagnosis of cirrhosis was based on 1) clinical criteria (an edema-ascitic syndrome with a heterogeneous hepatomegaly with a sharp inferior border or atrophic liver and a portal hypertension);2) biological criteria (a hepatocellular insufficiency syndrome);and 3) ultrasound imaging suggesting cirrhosis. Qualitative variables were presented as frequencies and percentages while quantitative ones were presented as means. <strong>Results:</strong> The data of 273 patients representing 33.9% of all hospitalizations were analyzed. The hepatobiliary diseases represented 74.7% of all diagnosis. The participants’ mean age was 46.9 years and the sex ratio, 2.7. The HBs antigen and anti-HCV antibodies were positive in 76.5% and 14.6% of the cases, respectively. Ascites was treated with puncture in 40.2% of patients, a salt-free diet (38.8%) and diuretics (54.2%). Hepatic encephalopathy was treated with lactulose in 73.6% of patients and two patients (1.3%) underwent esophageal varices ligation to treat their gastrointestinal bleeding. Lamivudine, tenofovir, and lamivudine-tenofovir combination were administered to 57.4%, 32.8%, and 10% of HBs Antigen positive patients, respectively. <strong>Conclusion:</strong> Cirrhosis still mostly affects the young and active male population at the gastro-enterology department of the university teaching hospital Yalgado Ouédraogo. Patients show up at a very advanced stage of the disease in poorly prepared health centres. In this context, prevention by the anti-hepatitis B immunization and early systematic screening with treatment when indicated are very efficient weapons at our disposal.</span> </div>展开更多
The diverse clinical presentation of Helicobacter pylori (H. pylori) infection results from the interaction between bacterial virulence, host genetics, socio-demographic and environmental factors. This study aimed to ...The diverse clinical presentation of Helicobacter pylori (H. pylori) infection results from the interaction between bacterial virulence, host genetics, socio-demographic and environmental factors. This study aimed to characterize Helicobacter pylori virulence genes and the associated behavioral factors among dyspeptic patients in Burkina Faso. Two hundred and fifty (250) stool samples were collected from patients with dyspepsia seen at health centers in Ouagadougou, Burkina Faso. Bacterial deoxyribonucleic acid (DNA) was extracted using a commercial kit. Virulence genes were detected using conventional multiplex Polymerase Chain Reaction with specific primers. The overall prevalence of Helicobacter pylori of the 250 participants was 91.20%. CagA virulence gene was present among 20.19% of individuals, while babA2 and vacA were detected respectively among 9.65% and 67.54% of the population positive for Helicobacter pylori. Among vacA subtypes, vacAs1 was the most frequent, with 39.04%, followed by vacAi1 (19.74%), vacAi2 (17.54%), and vacAs2 with 10.96%. Regarding vacAm1 and vacAm2, they were less frequent at 6.14% each. “Handwashing three times or less per day” significantly increased the risk of having vacAi2 allele and H. pylori rRNA16s, with p-values of 0.013 and 0.020, respectively. The consumption of non-tap water increases the risk of carrying the cagA virulence gene. Additionally, H. pylori-positive patients living with more than four (4) people in their household had about two times the risk of having the vacAs1 allele. The present study shows the detection of Helicobacter pylori cagA, vacA subtypes, and babA2 by stool a PCR method in Burkina Faso. The strong association between sanitary habits and virulence factors depicts the composite interaction between ecological factors, gastric mucosa, and bacteria. Therefore, the synergic action of these factors should be considered when aiming for bacterial eradication and gastric pathology cure.展开更多
文摘Fall Armyworms (FAW) Spodoptera frugiperda, particularly in maize crops, is a major agricultural pest. Current control methods rely heavily on chemical pesticides, which are costly and harmful to non-target organisms. To provide a sustainable alternative, the research isolated indigenous Entomopathogenic fungi, Metarhizium sp. and Trichoderma sp., from FAW-infested areas. The fungi were tested for virulence against FAW and a non-target insect, Cirina butyrospermi. Results showed that Metarhizium sp. FAW was highly effective in killing FAW while sparing Cirina butyrospermi, demonstrating its potential as a biological control agent. In addition, FAW infected with Metarhizium sp. exhibited a significant reduction in food consumption. The study highlights the potential use of indigenous fungi in integrated pest management systems, reducing the reliance on chemical pesticides and supporting sustainable agriculture. Further research is needed to understand the interaction of these promising Metarhizum strains with soil microbiota and their long-term efficacy in field conditions.
文摘Background: The Coronavirus disease 2019 (COVID-19) pandemic, caused by the SARS-CoV-2 coronavirus, remains a global threat despite lifting the health emergency. Scientists from all continents have been mobilized to develop vaccines and medicines for prevention and cure. In Burkina Faso, traditional healers proposed using Scoparia dulcis L., a medicinal plant, to manage COVID-19. Method: In silico screening offers a quick drug-likeness evaluation of Scoparia dulcis L.-isolated biomolecules toward SARS-CoV-2 targets, such as Mpro protease. A review of the literature retrieved 35 biomolecules isolated from Scoparia dulcis. The potential interactions of these biomolecules with the amino acid residues of the SARS-CoV-2 Mpro protease were visualized. Affinities and probable oral route delivery were assessed using reference molecules such as remdesivir and nelfinavir. Results: The screening allowed the retention of 20 hit molecules, which had a better affinity for the target than the reference molecules remdesivir and nelfinavir, and analysis of the results identified height lead molecules with a significant interaction with the Mpro protease and being druggable. There are six flavonoids: cirsimarin, cynaroside, hydroxy-tetramethoxyflavone, gossypetin, luteolin, vitexin, one diterpene, glutinol, and one glycoside, eugenyl-glucoside. These molecules interact with methionine 6 and tyrosine 126 of SARS-CoV-2 Mpro. These two amino acids are essential for the dimerization of Mpro protease. Inhibitory action on Mpro protease can be expected from these biomolecules. Conclusion: Scoparia dulcis L. could help manage COVID-19 because it contains biomolecules that can inactivate SARS-CoV-2 Mpro.
文摘One of the most promising vector control strategies for controlling dengue fever is the stable introduction of the obligate intracellular Wolbachia bacteria strain into Aedes aegypti mosquitoes. This method reduces the mosquito’s ability to transmit dengue through reproductive strategies associated with Wolbachia infection, such as parthenogenesis, male elimination or feminization, sex ratio distortions and cytoplasmic incompatibility. Expert knowledge and a risk assessment framework were used to identify the risks associated with the release of Wolbachia-bearing mosquitoes. Then, consultations with individual participants were organized and a Bayesian network (BN) was developed to capture the relationship between the hazards as well as the likelihood of these adverse events occurring. Finally, risk was calculated from the probability and consequence estimates obtained from our Burkina Faso participants, using a questionnaire based on the identified risks. Our “Cause More Harm” study yielded 46.15% negligible risk, 44.23% very low risk and 9.62% low risk. The “socio-cultural change” parameter had the greatest influence, with the perception that the dissemination project would be poorly received by the local population. This parameter alone accounted for 80% of the most significant risks. This explains the fact that hazard RA 49 “negative messages in social media” is ranked as the highest individual risk (although the risk is low) with a calculated risk of 0.261. The risk assessment was designed to integrate the interdependent complexity of hazards likely to affect the dissemination of technology in the environment. It represents an important implementation phase in the success of this innovative research, introducing a new technology to combat the transmission of dengue fever.
文摘Over the last few decades, dengue fever epidemics have increased in frequency and intensity worldwide, making it a major global concern for public health. Its prevention, which is essentially vector-based control, is already being compromised by reports of resistance of the main vector Aedes aegypti to insecticides. To tackle the rapid increase in insecticide resistance and outbreaks, the biological vector control is a promising approach. One of the strategies of this approach is the use of entomopathogenic fungi because of their great efficacy and their eco-friendly aspects. However, some aspects of their use, such as the low efficiency, the high cost of production and the sensitivity to various adverse conditions, need to be addressed for their successful large-scale application. Therefore, innovative technologies based on strains of transgenic fungi with improved biocontrol potentials by genetic engineering are actively pursued. Although these modified mycoinsecticides are acclaimed for their better effectiveness against target insects, the main concern remains their potential adverse effects on the environment and human health. The present review is dedicated to giving an update on recent developments in transgenic entomopathogenic fungi (TEF) for Aedes mosquito control. Future perspectives are also proposed to address the safety concerns related to the release of transgenic entomopathogenic fungi into the environment.
文摘In Burkina Faso, recent dengue outbreaks were exacerbated by Aedes aegypti mosquitoes, which have developed resistance to conventional insecticides. This study investigates the potential of Metarhizium pingshaense Met_S26, a fungus from Burkina Faso, for controlling both endophilic and exophilic Aedes mosquitoes. Traditional interventions are less effective against mosquitoes resting outdoors, and the ability of the fungus to be sexually transmitted could enhance its efficacy. Aedes mosquitoes were reared and exposed to various concentrations of Metarhizium pingshaense to assess its virulence. The fungus showed significant efficacy, with lethal times (LT80) ranging from 8.67 to 11.83 days depending on the concentration. Furthermore, bioassays demonstrated effective sexual auto-dissemination, with substantial transmission of fungal spores between infected and uninfected mosquitoes, leading to lower survival rates in uninfected mates. This study highlights Metarhizium pingshaense Met_S26 as a promising tool for controlling both indoor and outdoor Aedes populations, complementing existing vector control strategies such as the Sterile Insect Technique (SIT) and Wolbachia-based approaches. Future research should explore integrating this fungus with other control methods for a comprehensive vector management strategy.
文摘Malaria is a potentially lethal disease caused by parasites of the Plasmodium genus, transmitted to humans through the bite of infected female mosquitoes, primarily Anopheles. To control this disease, various strategies are implemented, including biological control, which targets the vectors of the parasite. This approach uses biological agents such as entomopathogenic fungi, including Metarhizium pingshaense, a fungus capable of causing lethal infections in mosquitoes. The production of Metarhizium pingshaense is still limited in Burkina Faso, and local cultivation of this fungus could help fill this gap. A study was conducted to identify optimal local substrates that promote its growth. Indeed, after gathering information on the dietary habits of populations in Bobo-Dioulasso and Bama, three potential substrates were selected: rice, cornmeal dough (MFL), and beans. These substrates were inoculated with two strains of Metarhizium pingshaense (S10 and S26) to assess their ability to support fungal growth and their effectiveness. Experimental results showed that MFL and bean substrates favored optimal growth of Metarhizium pingshaense, with growths of 1.91 cm and 2.13 cm after 8 days, compared to 1.83 cm on a standard media (PDA). In terms of virulence, S26 strain caused 60% mosquito mortality on both the bean and PDA media, while S10 strain induced mortalities of 50% for bean and 62% for PDA.
文摘Introduction: A recent study in Kenya highlighted a promising advance in malaria control by demonstrating that infecting mosquitoes with the endosymbiont Microsporidia MB blocks Plasmodium transmission. However, the influence of biotic and abiotic factors such as diet, relative humidity (RH) and temperature on this infection remains poorly studied. This study, aimed to gain a better understanding of this relationship. Methods: To highlight the influence of diet quantity, we defined a range of 3 quantities: 0.00375 g, 0.015 g and 0.09 g. Each quantity was tested on two groups of larvae: a group of 150 larvae infected with Microsporidia MB (MB+), and a group of 150 larvae not infected with Microsporidia MB (MB−) (control group), each divided into three replicates of 50 larvae. Each replicate was fed each morning with the assigned quantity until the pupal stage. In addition to this factor, we investigated the influence of temperature and RH. We defined three temperature-RH combinations: 21˚C-80% RH, 39˚C-50% RH, and 27˚C-75% RH. Each combination was tested on two groups of larvae: a group of 150 MB+ larvae and a group of 150 MB− larvae, each divided into three replicates of 50 larvae. Each replicate was subjected to the assigned combination until pupation. Pupae that had reached the adult stage were tested by PCR to determine their Microsporidia MB infection status for each factor studied. Results: The results showed that only the lowest quantity (0.00375 g) significantly reduced the prevalence of Microsporidia MB compared with the medium quantity (chi-2 test, χ2 = 4.9088, df = 1, p = 0.02672) and the high quantity (chi-2 test, χ2 = 4.7958, df = 1, p = 0.02853). As for temperature and RH, the combination 39˚C-50% RH led to a significant reduction in the prevalence of Microsporidia MB compared with the combination 27˚C-75% RH (chi-2 test, χ2 = 6.3736, ddl = 1, p = 0.01158) and that 21˚C-80% RH (chi-2 test, χ2 = 9.983, ddl = 1, p = 0.00158). Conclusion: This work contributes to a better understanding of some key factors linked to Microsporidia MB infection in mosquitoes. However, further research on several generations is necessary to draw more comprehensive conclusions.
文摘Cervical cancer is the fourth most common cancer worldwide, accounting for 6.8% of new cancer cases and 8.1% of cancer-related deaths. About 85% of these deaths occurred in low- and middle-income countries. The aim of this study was to assess the frequency and distribution of the human papillomavirus (HPV) genotypes in women showing cytological abnormalities of the cervix at the Sourô SANOU University Hospital (CHUSS) in Bobo-Dioulasso, Burkina Faso. This is a descriptive study of women recruited at the CHUSS. The cervico-uterine smear examination was carried out at the CHUSS Anatomy and Pathology Department for cervical cancer screening. The data were collected from women with atypical cells on their cervico-uterine smear. Cervicovaginal samples were taken from consenting women and HPV genotyping was performed using the HPV Direct FLOW CHIP kit at CERBA. We obtained approval from the ethics committee. The data were analyzed using the SPSS 26 software. The results of the study showed that 67.79% of the participants were aged between 50 and 65, a group that is particularly vulnerable to persistent infection with high-risk oncogenic HPV genotypes. Of the women screened, 40.7% were HPV positive and 29.2% had multiple infections. The most common genotypes were HPV 35, followed by HPV 18, 52, 58 and 66. These data highlight the need for increased surveillance and targeted prevention strategies among this female population.
文摘Background: In recent years, Burkina Faso has faced increasing insecurity and terrorist attacks. The police, gendarmerie, and justice services are very often in demand to carry out criminal investigations, which are slowed down or incomplete due to the absence of DNA analysis on biological samples from crime scenes and on apprehended suspects. The purpose of this study was to evaluate the contribution of DNA analysis to the resolution of criminal cases in Burkina Faso. Methodology: This study was carried out from June 2019 to July 2020. Three (03) crimes were investigated, and DNA analysis was performed on biological samples from the crime scene, suspects, and victims using the AmpFlSTR® identifiler® Direct kit on the ABI 3130 Genetic Analyzer. Results: In the explosion case, the alleles found in the victim were the same as those identified in the blood trace from the crime scene. In the armed robbery case, there was a perfect match between the DNA profile of the blood trail and that of suspect 2 for all 15 STRs analyzed. In the murder case, the DNA profile of the murdered man’s son and the DNA profile of the biological trace were identical. Conclusion: The DNA analyses carried out in criminal cases have identified the perpetrators of the crimes. Their guilt or innocence will be confirmed by the investigators during the interrogations and hearings.
文摘Introduction: Human pegivirus (HPgV), initially identified as hepatitis G virus in the 1990s, predominantly causes acute hepatitis and may persist particularly in individuals with compromised immune systems or those co-infected with HIV, HBV, or HCV. Despite its potential public health implications, particularly in transfusion contexts, comprehensive epidemiological data on HPgV in Burkina Faso remains scarce. Objectives: This study aimed to determine 1) the prevalence of human pegivirus infection among blood donors at the Regional Blood Transfusion Centre (Koudougou, Burkina Faso), and 2) the rates of co-infection between human pegivirus with HIV, HBV, HCV and Treponema pallidum. Material and Methods: Between 9 and 27 August 2022, 100 blood samples were collected and analyzed at the Regional Blood Transfusion Centre. Screening for HIV, HBV, HCV, and Treponema pallidum was conducted using the Cobas e 601 system (Roche Diagnostics). A 100 μL volume of each donor’s plasma was utilized for viral RNA extraction with the DNA/RNA Prep Kit (Sacace Biotechnologies) following the manufacturer’s instructions. HPgV RNA detection was conducted using the HGV Real-TM amplification kit (Sacace Biotechnologies). Results: The study was comprised of 100 blood donors, identifying HPgV RNA in 14 individuals (14% prevalence), with one noted co-infection with HBV. None of the participants were HIV positive. The prevalence rates for HBV and HCV were each found to be 5%, and syphilis also presented a prevalence of 5%. Conclusion: Our findings indicate a significant prevalence of HPgV among blood donors in Burkina Faso, underscoring the need for heightened surveillance and preventive measures in blood transfusion services and the broader population to enhance transfusion safety and public health.
文摘DNA testing for biological relationships is becoming increasingly common in Burkina Faso. STR analysis remains the most reliable technique for resolving disputes or claims in court regarding biological relationships. This study aimed to establish the links of biological relationships between subjects by analyzing 21 STR loci. The participants were 14 individuals referred to CERBA by the Justice in 2022. Oral or blood samples were taken for each subject. DNA was extracted, and the analysis of DNA polymorphism by PowerPlex® 21 Kit (Part No. DC8902) was performed by capillary electrophoresis on the ABI PRISM 3130 sequencer. DNA profiles were compiled using the GeneMapper IDX software version 1.2. Of the fourteen subjects examined, twelve of these samples had complete genetic profiles, while the other two had partial and absent profiles. The results confirmed the inclusion of three cases of Paternity, one case of maternity, one case of a relationship of brotherhood, and the exclusion of one case of maternity and one case of a relationship of brotherhood. DNA tests improve the resolution of filiations, but they require ethical and cultural awareness and a strengthened legal framework to prevent and protect society.
基金supported by the Bill&Melinda Gates Foundation(OPP1175213)supported by the Research Foundation Flanders(12X9620N and 12X9623N)the European Research Council(ERC)under the European Union’s Horizon 2020 research and innovation program(946192,HUMYCO)。
文摘Background:Evidence regarding the effectiveness of prenatal nutritional supplements has mainly considered anthropometric pregnancy outcomes.The effect on markers of health and disease,such as offspring telomere length(TL)and mitochondrial DNA content(mtDNAc)is unknown.Objectives:We assessed the efficacy of maternal multiple micronutrient(MMN)-fortified balanced-energy protein(BEP)and iron-folic acid(IFA)supplementation on newborn TL as a secondary outcome and mtDNAc as a non-declared outcome.Design:We conducted a randomized controlled trial in rural Burkina Faso,among pregnant females(15-40 years old)enrolled at<21 weeks of gestation.Mothers received either MMN-fortified BEP and IFA(intervention)or IFA only(control)throughout pregnancy.Whole arterial blood samples were collected from the umbilical cord of 104 control and 90 intervention group infants,respectively.Average relative TL and mtDNAc were measured using quantitative polymerase chain reaction.Linear regression models were fitted to assess TL and mtDNAc differences across trial arms.Results:We found that a combined daily MMN-fortified BEP supplement and IFA tablet did not affect newborn TL[β=-0.010(95%CI:-0.057,0.036);P=0.662]or mtDNAc[β=0.065(95%CI:-0.203,0.073);P=0.354],as compared to an IFA tablet alone.These findings were confirmed(P>0.05)by adjusting the regression models for potential prognostic factors of study outcomes at enrollment.Exploratory analyses indicated higher,but non-significantly different mtDNAc among children born either small-for-gestational age,low birthweight,or preterm.Conclusion:Newborns from mothers who received daily nutritional supplements across gestation did not have different relative TL or mtDNAc.
基金Supported by the Ministry of Health through FARES project(P1/FARES 2013)
文摘Objective:To evaluate the anthelmintic property of Saba senegalensis(A.DC)Pichon(Apocynaceae)(S.senegalensis)on Haemonchus contortus that is traditionally used in Burkina Faso for its gastrointestinal parasites treatment.Methods:The lyophilized aqueous decoction of leaves of S.senegalensis at concentrations of 0.10,1.00,3.00,10.00 and 15.00 mg/m L was used on eggs and adult worms of Haemonchus contortus collected from gastrointestinal tract of small ruminant.Results:The LC_(50)on adult worms was 6.79 mg/m L and 3.25 mg/m L for the leaves of S.senegalensis and the levamisole(reference drug),respectively.Inhibition of hatching assay showed a concentration-dependent manner with an inhibition of 93.63%at the concentration of 15.00 mg/m L of S.senegalensis.Conclusions:These results indicate that the aqueous extract of S.senegalensis possesses an anthelmintic property and may justify its use in traditional medicine for the treatment of gastrointestinal parasites.
文摘Background: Anastatica hierochuntica L. (Brassicaceae) has been claimed to treat various conditions, including complicated childbirth. The principal objective of this research was to provide scientific evidence on the contractile effect of the aqueous and hydroethanolic extracts of the whole plant from A. hierochuntica during labor. Method: Qualitative and quantitative methods helped identify and quantify the phytoconstituents in both extracts. The antioxidant activity was studied using four standard methods (ABTS, DPPH, FRAP, and LPO). Results: The inhibitory power of the extracts on pro-inflammatory enzymes such as 15-lipoxygenase and phospholipase A<sub>2</sub> was determined. The contractile effects of the extracts were evaluated using isolated pregnant mice uteri. Steroids, triterpenoids, flavonoids, tannins, coumarins, and reduced compounds were identified in both extracts. The hydroethanolic extract exhibited higher content of hydrolyzable tannins (15.07 ± 0.24 mg tannic acid equivalent/g) than the aqueous decoction (11.8 ± 0.69 mg TAE/g). The extracts demonstrated the ability to scavenge DPPH and ABTS radicals, reduce ferric ions, and inhibit especially lipid peroxidation. No significant difference was noted in the phospholipase inhibition between the extracts and betamethasone. The hydroethanolic extract displayed the most significant anti-lipoxygenase activity with an IC<sub>50</sub> value of 55.82 ± 1.15 μg/mL. The lethal dose (LD<sub>50</sub>) of extracts was estimated to be superior to 2000 mg/kg body weight (bw). The ability of the samples to produce one cm amplitude of contraction at 50% effective concentrations (EC<sub>50</sub>) was 5.33 × 10<sup>−8</sup> ± 0.32 mg/mL for oxytocin, 1.51 ± 0.08 mg/mL for aqueous extract and 3.57 ± 0.61 mg/mL for hydroethanolic extract. Conclusion: These findings could provide evidence for using Anastatica hierochuntica to facilitate childbirth.
文摘<strong>Objective:</strong> The aim of this study was to assess the level of erythrocyte membrane cholesterol in sickle cell patients, which is one of the essential parameters of membrane fluidity that contributes to understanding the hemolytic state of the erythrocyte. <strong>Methods: </strong>We worked with blood specimens from 20 controls and 50 sickle cell patients. The blood count and the isoelectric focusing (IEF) were performed on the samples in order to select them. The titration of the erythrocyte membrane cholesterol was made after washing and lysing the erythrocytes with the hemolyzing solution (EDTA, 2 Mercapto-Ethanol, NADP, NaOH). The cholesterol level was assessed by the enzymatic colorimetric method. The results were analyzed by Student’s test. <strong>Results: </strong>We worked with 16 control subjects with a hemoglobin status and a normal hemogram. The evaluation of the erythrocyte membrane cholesterol level of the samples allows us to define the reference interval (<em>α</em> = 0.05) at 17.55 ± 3.83 mg of cholesterol/g of hemoglobin. The erythrocyte membrane cholesterol levels of sickle cell patients found in this study were 11.58 ± 2.98 mg cholesterol/g of hemoglobin. In this study, 38 sickle cell patients (76%) were found with a low erythrocyte membrane cholesterol level compared to the reference interval. Statistical analysis showed that there was a significant difference (<em>α </em>= 5%) between the erythrocyte membrane cholesterol level of normal subjects and sickle cell patients. <strong>Conclusion: </strong>Most of the sickle cells patients had a decreased erythrocyte membrane cholesterol level. This reduces the membrane fluidity making the erythrocyte membrane rigid and more fragile. This may be one of the factors responsible for the lyses of erythrocytes.
文摘<div style="text-align:justify;"> <span style="font-family:Verdana;"><strong>Introduction: </strong>Cirrhosis represents 27.63% of the hepatobiliary diseases in Burkina Faso. We aimed at studying the therapeutic and evolutionary features of the cirrhosis. <strong>Patients and methods: </strong>We implemented a cross-sectional and descriptive study, collecting retrospectively the data from 1st January 2012 to 31st March 2014. The diagnosis of cirrhosis was based on 1) clinical criteria (an edema-ascitic syndrome with a heterogeneous hepatomegaly with a sharp inferior border or atrophic liver and a portal hypertension);2) biological criteria (a hepatocellular insufficiency syndrome);and 3) ultrasound imaging suggesting cirrhosis. Qualitative variables were presented as frequencies and percentages while quantitative ones were presented as means. <strong>Results:</strong> The data of 273 patients representing 33.9% of all hospitalizations were analyzed. The hepatobiliary diseases represented 74.7% of all diagnosis. The participants’ mean age was 46.9 years and the sex ratio, 2.7. The HBs antigen and anti-HCV antibodies were positive in 76.5% and 14.6% of the cases, respectively. Ascites was treated with puncture in 40.2% of patients, a salt-free diet (38.8%) and diuretics (54.2%). Hepatic encephalopathy was treated with lactulose in 73.6% of patients and two patients (1.3%) underwent esophageal varices ligation to treat their gastrointestinal bleeding. Lamivudine, tenofovir, and lamivudine-tenofovir combination were administered to 57.4%, 32.8%, and 10% of HBs Antigen positive patients, respectively. <strong>Conclusion:</strong> Cirrhosis still mostly affects the young and active male population at the gastro-enterology department of the university teaching hospital Yalgado Ouédraogo. Patients show up at a very advanced stage of the disease in poorly prepared health centres. In this context, prevention by the anti-hepatitis B immunization and early systematic screening with treatment when indicated are very efficient weapons at our disposal.</span> </div>
文摘The diverse clinical presentation of Helicobacter pylori (H. pylori) infection results from the interaction between bacterial virulence, host genetics, socio-demographic and environmental factors. This study aimed to characterize Helicobacter pylori virulence genes and the associated behavioral factors among dyspeptic patients in Burkina Faso. Two hundred and fifty (250) stool samples were collected from patients with dyspepsia seen at health centers in Ouagadougou, Burkina Faso. Bacterial deoxyribonucleic acid (DNA) was extracted using a commercial kit. Virulence genes were detected using conventional multiplex Polymerase Chain Reaction with specific primers. The overall prevalence of Helicobacter pylori of the 250 participants was 91.20%. CagA virulence gene was present among 20.19% of individuals, while babA2 and vacA were detected respectively among 9.65% and 67.54% of the population positive for Helicobacter pylori. Among vacA subtypes, vacAs1 was the most frequent, with 39.04%, followed by vacAi1 (19.74%), vacAi2 (17.54%), and vacAs2 with 10.96%. Regarding vacAm1 and vacAm2, they were less frequent at 6.14% each. “Handwashing three times or less per day” significantly increased the risk of having vacAi2 allele and H. pylori rRNA16s, with p-values of 0.013 and 0.020, respectively. The consumption of non-tap water increases the risk of carrying the cagA virulence gene. Additionally, H. pylori-positive patients living with more than four (4) people in their household had about two times the risk of having the vacAs1 allele. The present study shows the detection of Helicobacter pylori cagA, vacA subtypes, and babA2 by stool a PCR method in Burkina Faso. The strong association between sanitary habits and virulence factors depicts the composite interaction between ecological factors, gastric mucosa, and bacteria. Therefore, the synergic action of these factors should be considered when aiming for bacterial eradication and gastric pathology cure.
