Mycotoxins are the toxic metabolites produced by the fungi that grow in food crops;however,the modified mycotoxins are the undetectable metabolites during testing for the parent mycotoxin.Although more than 300 mycoto...Mycotoxins are the toxic metabolites produced by the fungi that grow in food crops;however,the modified mycotoxins are the undetectable metabolites during testing for the parent mycotoxin.Although more than 300 mycotoxins were discovered and identified,little is known about the effects of most of them or their modified forms on child health.Aflatoxins are well known to induce liver cancer and play a significant role in the impairment of child growth.Fumonisin also are known to induce esophageal cancer and neural tube defects.However,deoxynivalenol and the other trichothecenes are well known as immunotoxic and induce gastroenteritis.In this review,the adverse health hazards in children associated with the major mycotoxins as well as the modified forms of fusarium mycotoxins were described.Additionally,the global burden of the health ghazards resulted from the exposure of children to dietary mycotoxin.The risk assessment,risk characterization and the challenges in risk characterization of multiple mycotoxins in children food were discussed.展开更多
Cornus officinalis Sieb.et Zucc.,a valuable Chinese medicine resource,has a long clinic utilization history.In recent years,more research focus on the chemical composition and pharmacological activity of C.officinalis...Cornus officinalis Sieb.et Zucc.,a valuable Chinese medicine resource,has a long clinic utilization history.In recent years,more research focus on the chemical composition and pharmacological activity of C.officinalis which was used as health foods and drugs.This paper summarized the active ingredients,pharmacological activities and molecular mechanisms of C.officinalis in recent 5 years to provide reference for the development and utilization of C.officinalis.A total of 149 active constituents of C.officinalis were summarized,including iridoids(64),flavonoids(18),lignin(17),tannins(16),organic acids and phenolic acids(14),triterpenes(11)and others(9),which were further subdivided according to their pharmacological effects.It was found that the pharmacological effects of C.officinalis were very extensive,mainly including antioxidant,anti-inflammatory,anti-tumor,neuroprotective and other biological activities.Among these activities,the anti-inflammatory and hypoglycemic mechanisms showed multi-pathway and multi-target characteristics,which were elaborated systematically.However,there are still many compounds in C.officinalis that have not been studied for biological activity,which means that it still has potential pharmacological activity to be further studied.C.officinalis not only has high medicinal value,but also has a potential edible resource.However,limited by people's understanding of the nutritional value of C.officinalis,few product types and incomplete processing technology,people's acceptance of the edible value of C.oficinalis is not high,and it needs to be further developed and utilized.展开更多
Flower plants are popular all over the world and important sources of ornamental plants,bioactive molecules and nutrients.Flowers have a wide range of biological activities and beneficial pharmacological effects.Flowe...Flower plants are popular all over the world and important sources of ornamental plants,bioactive molecules and nutrients.Flowers have a wide range of biological activities and beneficial pharmacological effects.Flowers and their active ingredients are becoming more and more popular in the preparation of food,drugs and industrial products.This paper summarizes the active ingredients,pharmacological activities and applications in the pharmaceutical and food industries of flower plants in recent years.In addition,the possible molecular mechanism of pharmacological effects of flower plants were also discussed.302 active constituents from 55 species of flower plants were summarized,including flavonoids(115),terpenoids(90),phenylpropanoids(20),alkaloids(13),organic acids(27)and others(37).The pharmacological effects of flower plants are very extensive,mainly including antioxidant,anti-inflammatory,anti-tumor,anti-virus,and hypoglycemic.The mechanisms of anti-inflammatory,anti-tumor and hypoglycemic activities present the characteristics of multi-way and multi-target.Because of its rich nutrients,bioactive ingredients and plant essential oils,and its wide sources,flower plants are widely used in food,beverage,cosmetics and drug research.Flower plants also play an important role in pharmaceutical industry,food industry and other fields.展开更多
Proteomics is a new technology that has been widely applied in the field of life and health science.It effectively addresses issues related to the impact of dietary structure on organs,tissues,and cells,as well as the...Proteomics is a new technology that has been widely applied in the field of life and health science.It effectively addresses issues related to the impact of dietary structure on organs,tissues,and cells,as well as the changes in proteins in various organs,tissues,and cells under disease conditions.The differential proteins identified through proteomics can serve as disease biomarkers and target proteins affecting health and can be used for disease diagnosis and health regulation.In this paper,the application of proteomics in the field of infl ammation in recent years was summarized,especially in the therapeutic target and mechanism of action,which opens up a new way for more effective prevention,diagnosis,and treatment of inflammation,and provides medical protection for human life and health.展开更多
Fusarium graminearum(F.graminearum)is a severe phytopathogen threatening agriculture production and food security.Paeonol,serves as a plant-derived natural component,is a promising antifungal agent.At a concentration ...Fusarium graminearum(F.graminearum)is a severe phytopathogen threatening agriculture production and food security.Paeonol,serves as a plant-derived natural component,is a promising antifungal agent.At a concentration of 0.3125 mg/mL,paeonol was adequate to fully inhibit the growth of F.graminearum mycelia within 3 days.Fourier-Transform Infrared Spectroscopy(FT-IR)analysis showed that paeonol had no impact on the outer surface of F.graminearum cell walls.While propidium iodide staining,extracellular conductivity,and pH value measurements demonstrated that paeonol disrupted the cell membrane.Furthermore,lipid oxidation and osmotic stress responses were observed in F.graminearum treated with paeonol,resulting in a 47.23%rise in malondialdehyde(MDA)levels and a 515.43%increase in glycerol levels.Moreover,on the 7th day after exposure to paeonol treatment,the deoxynivalenol(DON)level was significantly reduced,measuring only onefifth of that in the control group.Finally,paeonol was shown to inhibit F.graminearum on wheat grains and steamed bread slices.These results,for the first time,revealed the inhibitory mode of action of paeonol against F.graminearum as reflected by disruption of cell membrane integrity,induction of lipid oxidation and osmotic pressure,as well as DON biosynthesis.Furthermore,this study provided scientific evidence for the potential applications of paeonol in agriculture and food industry.展开更多
Synthesis of zinc oxide nanoparticles(ZnO-NPs)via green method is an outstanding alternative to conventional/regular methods;however,the safety or toxicity of the biosynthesized ZnO-NPs in vivo is not fully explored.T...Synthesis of zinc oxide nanoparticles(ZnO-NPs)via green method is an outstanding alternative to conventional/regular methods;however,the safety or toxicity of the biosynthesized ZnO-NPs in vivo is not fully explored.This study was conducted to evaluate the protective efficiency of cinnamaldehyde-loaded chitosan nanoparticles(Cin@CSNPs)against oxidative damage and genotoxicity of ZnO-NPs in mice.ZnO-NPs were biosynthesized using the extract of fresh leaves of Mentha pulegium L.Cin was extracted from cinnamon essential oil,and was loaded into chitosan nanoparticle(Cin@CSNPs).Both ZnO-NPs,Cin@CSNPs and CSNPs were characterized.The in vitro release of Cin@CSNPs was determined.In the biological study,6 groups of male BALB/c mice were treated by gavage for 3 weeks as follows,control group,the group received ZnO-NPs(25 mg/kg b.w),the groups received Cin@CSNPs at low dose(50 mg/kg b.w)or high dose(100 mg/kg b.w),and the groups received ZnO-NPs plus Cin@CSNPs at the 2 tested doses.Blood and tissue samples were collected for different biochemical,genetical and histological studies.The particle size of ZnO-NPs,CSNPs,and Cin@CSNPs were(20.78±2.60),(170.0±3.7),and(218.23±2.90)nm,andξ-potential were(32.7±4.6),(8.32±0.27)and(4.80±0.21)mV,respectively.ZnO-NPs disturbed the biochemical and oxidative stress indices,AFP,CEA,TNF-α,chromosomal aberrations in somatic and germ cells,and sperm abnormality along with severe pathological changes in the hepatic,renal,and testicular tissues.Cin@CSNPs improved significantly all the parameters tested and the histological picture in a dose-dependent.Therefore,the biosynthesized ZnO-NPs exhibit oxidative damage and genotoxicity,and Cin@CSNPs have potential protective effects against the risks of ZnO-NPs and may be a promising tool to overcome the challenges of using Cin in food and pharmaceuticals applications.展开更多
Memory deficits are a significant societal issue.The study sought to examine the specific distribution patterns and the regulatory proteins and pathway mechanisms of Antarctic krill tripeptide Phe-Pro-Phe(FPF)in mitig...Memory deficits are a significant societal issue.The study sought to examine the specific distribution patterns and the regulatory proteins and pathway mechanisms of Antarctic krill tripeptide Phe-Pro-Phe(FPF)in mitigating scopolamine-induced memory deficits in mice.FPF significantly enhanced behavioral performance,elevated acetylcholine(ACh)levels and unsaturated lipid concentrations,while reducing acetylcholinesterase(AChE)activity and neuronal damage,as evaluated through behavioral studies,Nissl staining,and Fourier transform infrared imaging(FTIR).Additionally,it was shown that FPF might be directed towards the hippocampus.The tissue distribution was as follows:duodenum>jejunum>ileum>hippocampus>kidney,liver,heart,lung,and spleen,employing Liquid Chromatography-Tandem Mass Spectrometry(LC-MS/MS).Proteomics and immunofluorescence analyses indicated that FPF markedly elevated the expression levels of MT-ATP8,SST,and CCS proteins,optimized energy metabolism homeostasis,augmented neural signaling efficiency,and improved synaptic plasticity,thereby exerting a neuroprotective effect and mitigating scopolamine-induced memory deficits.展开更多
The polysaccharides from edible fungus showed many kinds of biological activities,including anti-tumor,immunomodulatory,anti-inflammatory,anti-diabetes,improving functional constipation activities.In particular,the im...The polysaccharides from edible fungus showed many kinds of biological activities,including anti-tumor,immunomodulatory,anti-inflammatory,anti-diabetes,improving functional constipation activities.In particular,the immunomodulatory effects have been paid more and more attention by scholars,but there was no systematic introduction of their immunomodulatory mechanism.So,this review introduced the immunomodulatory effects and mechanism of edible fungus polysaccharides in recent years,and then the relationships between structure and immunomodulatory effect were also discussed.展开更多
Two immunomodulatory polysaccharides(Vp2a-Ⅱ and Vp3) were isolated and identified from Apocynum venetum L. flowers, and their innate immune-stimulating functions and working mechanisms were evaluated in RAW264.7 cell...Two immunomodulatory polysaccharides(Vp2a-Ⅱ and Vp3) were isolated and identified from Apocynum venetum L. flowers, and their innate immune-stimulating functions and working mechanisms were evaluated in RAW264.7 cells. Both the level of released nitric oxide(NO) and expression of inducible nitric oxide synthase(iNOS) m RNA were significantly enhanced in the RAW264.7 macrophages cells treated by Vp2a-Ⅱ and Vp3. Vp2a-Ⅱ(100–800 μg/m L) and Vp3(400 μg/mL) could significantly increase the phagocytic activity of RAW264.7 cells and the secretion and m RNA expression of TNF-α and IL-6 in a concentrationdependent manner through affecting mitogen-activated protein kinase(MAPK) activity and nuclear factor κB(NF-κB) nuclear translocation. Vp2a-Ⅱ might activate the MAPK signaling pathways and induce the nuclear translocation of NF-κB p65, whilst Vp3 likely activated the NF-κB and MAPK signaling pathways without influencing the p38 MAPK route.展开更多
This study was designed to elucidate the immunoregulation of Ganoderma lucidum.HPLC fingerprint and spectrum-effect relationship of G.lucidum were established to predict the active compounds and BP neural network mode...This study was designed to elucidate the immunoregulation of Ganoderma lucidum.HPLC fingerprint and spectrum-effect relationship of G.lucidum were established to predict the active compounds and BP neural network model was established to predict the efficacy.Then the target compounds were identified by high resolution mass spectrometry.The results indicated that there are both enhanced immunity and immunosuppressive components in G.lucidum.BP neural network was trained with the common peak area and immune effi cacy index of G.lucidum fi ngerprint as samples,and a combined evaluation system of G.lucidum fi ngerprint effi cacy was established.The correlation coeffi cient R of BP network model was 0.98643,and the error of pharmacodynamic prediction results was in the ideal range.Eight compounds were identifi ed by high resolution mass spectrometry.The compounds related to immune activity in G.lucidum were determined in this study.展开更多
Chestnut is a high nutritional value food that has been widely used as a tonic in traditional Chinese medicine.As an emerging functional food ingredient,Chinese chestnuts are rich in a range of bioactive nutrients suc...Chestnut is a high nutritional value food that has been widely used as a tonic in traditional Chinese medicine.As an emerging functional food ingredient,Chinese chestnuts are rich in a range of bioactive nutrients such as starch,dietary fiber,fat,protein,trace metal element and vitamins A,B,C,D and other nutrients.In our study,Chinese chestnut powder(CCP)were added into bread formulation at 2%-6%levels(based on flour weight)to produce fresh bread with enhanced anti-staling characteristics and starch digestion inhibitory ability.The texture properties,retrogradation enthalpy,water distribution,and estimated glycemic index(eGI)of wheat bread containing CCP as a functional additive were also investigated.The results showed that incorporation of CCP apparently affected bread texture,resulting in increased hardness,as well as decreased the specific volume of wheat bread.These influences were generally proportional to the amount of CCP used.It was found that adding too much CCP resulted in a dark red color,showing increased significantly higher total color difference(ΔE)and L values.Conversely,addition of CCP significantly reduced starch digestion rate and digestion extent in bread,and the reduction degree was positively related to the amount of CCP applied.The greatest reduction in eGI value from 79.40(control)to 75.02(6%CPP bread)was observed.Meanwhile,the content of resistant starch of 6%CPP bread was about 1.36 times higher than that of control bread.CCP also reduced crumb water loss and drove the water shift from the bound to the mobile state after stored for 7 days.The retrogradation enthalpy analyses further confirmed that CCP inhibited starch retrogradation and recrystallization.These results suggested that Chinese chestnut powder could be incorporated into fresh bread to provide health functions,such as lowering potential glycaemic response and improving anti-staling characteristics of bread.展开更多
[Objectives] This study was conducted to optimize the determination conditions of amino acids from abalone. [Methods] The sample was treated by acid hydrolysis method and subjected to 2,4-2 nitro fluorobenzene column ...[Objectives] This study was conducted to optimize the determination conditions of amino acids from abalone. [Methods] The sample was treated by acid hydrolysis method and subjected to 2,4-2 nitro fluorobenzene column derivatization. The amino acid content in abalone was determined by HLPC,and the nutritional value of the amino acids was evaluated with egg protein model put forward by Institute of Nutrition and Food Hygiene,Chinese Academy of Preventive Medicine. [Results] Abalone contains full amino acids. According to the FAO/WHO ideal,it is a high-quality protein source and suitable for supplement of protein source for human body. [Conclusions]The experimental method has simple operation and could achieve a good effect with wide linear range and correlation coefficient over 0. 999 8,and the obtained results are satisfactory.展开更多
Patrinia scabiosaefolia,is used as wild vegetable in China for more than 2000 years,with a variety of pharmacological activities,including anti-inflammatory,anti-tumor and hypoglycemic.Based on our ongoing research on...Patrinia scabiosaefolia,is used as wild vegetable in China for more than 2000 years,with a variety of pharmacological activities,including anti-inflammatory,anti-tumor and hypoglycemic.Based on our ongoing research on chemical constituents and hypoglycemic activity of P.scabiosaefolia,4 lignan compounds,(+)-isolariciresinol(1),7R,7’R,8S,8’S-(+)-neo-olivil-4-O-β-D-glucopyranoside(2),4-O-methylcedrusin(3)and patrinian A(4),were isolated and identifi ed.The hypoglycemic activity showed that compounds 2 and 3 could extremely signifi cantly improve insulin resistance at 100(P<0.001),50(P<0.001)and 25μmol/L(P<0.01)in IR 3T3-L1 cells.While compound 4 only promoted glucose uptake by IR 3T3-L1 cells at 100μmol/L(P<0.01).Western blotting experiments showed that compounds 2 and 4 up-regulated the protein expressions of p-IRS,PI-3K,p-AKT and glucose transporter 4(GLUT4),and promoted the transcription of GLUT4 mRNA.Therefore,the mechanisms of compounds 2 and 4 were presumed to improve IR by activating PI-3K/AKT signaling pathway.展开更多
The Escherichia coli(E.coli)is prevailing worldwide,but the epidemiology of E.coli infections feature regional distribution characteristics to some extent.E.coli,as a zoonotic pathogen,can be transferred from animals ...The Escherichia coli(E.coli)is prevailing worldwide,but the epidemiology of E.coli infections feature regional distribution characteristics to some extent.E.coli,as a zoonotic pathogen,can be transferred from animals to humans through food chain or via contact with wounds,causing a public health risk.We reported the swelling of proventriculus and tracheal bleeding following the death in two broiler chickens(Gallus gallus domesticus)from Beijing,China.To investigate whether a virus was involved in the infection,Madin Darby Bovine Kidney(MDCK)cells were co-cultured with supernatants of proventriculus,trachea and spleen homogenates.The avian leucosis virus was detected in the samples of proventriculus and trachea,but the avian influenza virus,the Newcastle disease virus and the avian infectious laryngotracheitis virus were not detected.E.coli isolates were resistant to almost all the antimicrobial as tested except for the combinations of amoxicillin/clavulanic acid and sulfamethoxazole/trimethoprim.PCR tests demonstrated the presence of antibiotic resistance genes in these E.coli isolates and further research revealed a novel gene profile with the presence of CTX-M-1,gyrA,gyrB,oqxA,oqxB,parC and Sul2 antibiotic resistance genes in a strain isolated from a proventriculus sample.These results demonstrated that the presence of antibiotic resistant E.coli would not necessarily cause outbreak of large-scale disease.However,when the bacteria carrying new antibiotic resistance genes enter the environment,it may result in the development of more virulent strains which will potentially impact human and animal health.展开更多
In recent years,Volvariella polysaccharides(VPs)have been confirmed with various of biological activities,which can be used as the main ingredients of functional foods.it was reported to treat various diseases and dis...In recent years,Volvariella polysaccharides(VPs)have been confirmed with various of biological activities,which can be used as the main ingredients of functional foods.it was reported to treat various diseases and disorders in humans,and have high nutritional and pharmaceutical significance.this paper summarized the latest progress in the extraction,separation and purification technologies,structure-activity relationship and action mechanisms of VPs,as well as the challenges,limitations during the application that may become the future direction.展开更多
Fungal contamination and mycotoxin production pose significant challenges to maize preservation.In response,we developed ZnO@SiO2 nanocomposite,with zinc oxide nanoparticles as the core and mesoporous silica encapsula...Fungal contamination and mycotoxin production pose significant challenges to maize preservation.In response,we developed ZnO@SiO2 nanocomposite,with zinc oxide nanoparticles as the core and mesoporous silica encapsulation.Transmission Electron Microscope(TEM)confirmed uniform morphology and good dispersion.Energy Dispersive Spectroscopy(EDS),Fourier Transform Infrared Spectroscopy(FT-IR),and X-Ray Diffraction(XRD)analyses validated the successful fabrication.Assessing the mycelial growth and mycotoxin production of Aspergillus flavus and Fusarium graminearum,two predominant fungi during maize storage,Significant inhibition by the nanocomposites was observed in both maize flour medium and sterile kernels.In maize flour,it sup-pressed A.flavus and F.graminearum toxin production by over 64.11%and 80.53%,respectively.In maize kernels,DON,ZEN,AFB1,AFB2,AFG1,and AFG2 toxins were all inhibited by over 81.3%.Nanocomposite’s effectiveness endured in practical storage,with inhibition rates exceeding 72.03%at an addition concentration of 3.0 g/kg.These findings suggest nanocomposite as a promising tool for mitigating mycotoxin contamination during maize storage,with implications for food safety enhancement.展开更多
Soy,peanut and chickpea contain polyphenols that have multiple beneficial biological activities.In this work,two Lactobacillus strains were employed to ferment the three kinds of bean milk and the effects of fermentat...Soy,peanut and chickpea contain polyphenols that have multiple beneficial biological activities.In this work,two Lactobacillus strains were employed to ferment the three kinds of bean milk and the effects of fermentation on the polyphenols in them were investigated.After fermentation with L.fermentum GD01 and L.plantarum BHP03,the total phenolic contents were increased by 16.90% and 14.64%in soy milk,18.14%and 24.28%in peanut milk,46.39%and 51.91%in chickpea milk,respectively.Specifically,the contents of free phenol,insoluble ester phenol,and insoluble glycosidic phenol increased,while the contents of soluble ester phenol and soluble glycosidic phenol reduced.These changes were positively associated with the antioxidant activities.Further,Lactobacillus fermentation also changed the contents and forms of monomeric phenols in bean milk,with the free monomeric phenols exhibiting the greatest diversity in types.In addition,the fermented bean milk demonstrated a higher release of phenols compared to the unfermented counterparts during simulated digestion,resulting in enhanced antioxidant activities.Notably,the most substantial increase in soluble phenols was detected in fermented chickpea milk(162.66%by GD01,137.48%by BHP03),while the most significant increase in insoluble phenols was observed in fermented peanut milk(147.46%by GD01,150.53%by BHP03).All the results confirmed that Lactobacillus fermentation could alter the structures,compositions,digestive characteristics of the polyphenols in the three kinds of bean milk,ultimately enhancing their antioxidant activities.These results offer a theoretical foundation for the advancement of fermented bean milk products with enhanced biological activity.展开更多
Lactobacillus is the dominant bacterial group in vaginal microbiota,which can inhibit the growth of pathogenic bacteria and maintain the balance of the vaginal microbiota.To develop vaginal probiotics with efficient a...Lactobacillus is the dominant bacterial group in vaginal microbiota,which can inhibit the growth of pathogenic bacteria and maintain the balance of the vaginal microbiota.To develop vaginal probiotics with efficient anti-bacterial effects,in this work,the antibacterial activities of the metabolites from Lactobacillus crispatus BHC04 and L.delbrueckii Q5.4 against three indicator bacteria(Escherichia coli ATCC 8739,Staphylococcus aureus ATCC 25923,Candida albicans CMCC(F)98001)were investigated by determining the growth curves and time-killing curves.And the metabolites of the two strains could completely inhibit the growth of the indicator bacteria.Combined the investigation of confocal laser scanning microscope,flow cytometry and scanning electron mi-croscope,it could be confirmed that the metabolites damaged the cell walls and cell membranes of the indicator bacteria.Metabolites also increased the ROS levels,decreased the fatty acid contents of cell membrane and changed the membrane protein structures in the indicator bacteria as analyzed by FT-IR spectroscopy.Furthermore,the metabolites down-regulated the expression of fatty acid synthesis genes(fabA,fabD,fabG,fabI)in E.coli cell membrane and up-regulated the transcription factors(rpos),which resulted in the synthesis of cyclic fatty acids to cope with the stress of metabolites treatment.All the results revealed that the antibacterial mechanisms of the metabolites were to destroy cellular integrity,disrupt antioxidant system,weaken strength of cell membrane and cause cellular protein misfolding.This work would provide a theoretical support for developing Lactobacillus as functional foods beneficial to gynecological health.展开更多
Food allergy(FA)has caused significant impacts on patients'quality of life.This study comprehensively investigated the therapeutic and prophylactic effects of Lacticaseibacillus paracasei BHP06 on an ovalbumin(OVA...Food allergy(FA)has caused significant impacts on patients'quality of life.This study comprehensively investigated the therapeutic and prophylactic effects of Lacticaseibacillus paracasei BHP06 on an ovalbumin(OVA)-induced allergy model,while elucidating its immunomodulatory mechanisms.The results showed that compared with the model group,mice in high-dose prophylaxis group(PH6 group)exhibited lower clinical scores for allergic symptoms and decreased levels of OVA-specific IgE(OVA-sIgE),OVA-sIgG1,histamine,mouse mast cell protease(mMCP)1,and interleukin(IL)4 by 52.00%,54.43%,18.82%,6.48%,and 29.04%,respectively.Meanwhile,the PH6 group demonstrated significant mitigation of jejunal inflammation,and decrease in the number of mast cells in the intestines of mice.In addition,oral administration of L.paracasei BHP06 maintained immune organ homeostasis in allergic mice,decreased gene expression of IL-4,TNF-α,and GATA-3 in the spleen,increased gene expression of IL-10,T-bet,Foxp3,and TGF-β,and modulated the expression of genes associated with the TLR4/NF-κB signaling pathway.16S rDNA sequencing analysis revealed that the intake of L.paracasei BHP06 enhanced the ratio of Firmicutes/Bacteroidota in the gut,leading to significant alterations in the diversity.In summary,oral administration of L.paracasei BHP06 exhibited multiple beneficial effects in alleviating FA by regulating immune balance,intestinal inflammation,TLR4/NF-κB signaling pathway,and intestinal flora.Additionally,the preventive intervention strategy using L.paracasei BHP06 demonstrates a more comprehensive and stable effect.These findings suggested the potential development of L.paracasei BHP06 as a functional food for the prevention and management of FA.展开更多
The peptide Ser-Phe-Gly-Asp-Ile(SFGDI),derived from sea cucumber,has demonstrated neuroprotective effects in cell models;however,the in vivo neuroprotective efficacy of SFGDI remains unexplored.In this study,we utiliz...The peptide Ser-Phe-Gly-Asp-Ile(SFGDI),derived from sea cucumber,has demonstrated neuroprotective effects in cell models;however,the in vivo neuroprotective efficacy of SFGDI remains unexplored.In this study,we utilized a cognitive disorder mouse model induced by scopolamine and observed that daily oral administration of SFGDI at a dosage of 40 mg/kg resulted in improved behavioral performance in the Passive avoidance test,the Channel-type water maze test,and the Novel object recognition test(P<0.05).Furthermore,our investigation into the underlying neuroprotective mechanisms of SFGDI revealed that it reduced oxidative stress levels through the activation of the Sirt3/SOD/ROS pathway,thereby balancing hippocampal unsaturated lipids(P<0.05)in the mouse hippocampus.Moreover,SFGDI significantly decreased hippocampal neuroinflammation by modu-lating the activation of microglia and hypertrophy of astrocytes.Additionally,SFGDI was found to enhance synaptic plasticity in the mouse hippocampus by activating the ChAT/p-CaMKII/BDNF pathway and increasing spine density in neuron cells(P<0.05),particularly mushroom spines(P<0.05).These experiments demon-strate that SFGDI exhibits neuroprotective effects by regulating hippocampal oxidative stress through the Sirt3/SOD/ROS pathway and synaptic plasticity via the ChAT/p-CaMKII/BDNF pathway in the mouse hippocampus,thus emerging as a potential neuro-nutraceutical agent for alleviating memory impairment.展开更多
文摘Mycotoxins are the toxic metabolites produced by the fungi that grow in food crops;however,the modified mycotoxins are the undetectable metabolites during testing for the parent mycotoxin.Although more than 300 mycotoxins were discovered and identified,little is known about the effects of most of them or their modified forms on child health.Aflatoxins are well known to induce liver cancer and play a significant role in the impairment of child growth.Fumonisin also are known to induce esophageal cancer and neural tube defects.However,deoxynivalenol and the other trichothecenes are well known as immunotoxic and induce gastroenteritis.In this review,the adverse health hazards in children associated with the major mycotoxins as well as the modified forms of fusarium mycotoxins were described.Additionally,the global burden of the health ghazards resulted from the exposure of children to dietary mycotoxin.The risk assessment,risk characterization and the challenges in risk characterization of multiple mycotoxins in children food were discussed.
基金supported by National Key R&D Program of China(2022YFF1100300)Major Science and Technology Project of Henan Province(231100310200)+1 种基金Key R&D Project of Henan Province(231111112100)Research on Precision Nutrition and Health Food,Department of Science and Technology of Henan Province(CXJD2021006).
文摘Cornus officinalis Sieb.et Zucc.,a valuable Chinese medicine resource,has a long clinic utilization history.In recent years,more research focus on the chemical composition and pharmacological activity of C.officinalis which was used as health foods and drugs.This paper summarized the active ingredients,pharmacological activities and molecular mechanisms of C.officinalis in recent 5 years to provide reference for the development and utilization of C.officinalis.A total of 149 active constituents of C.officinalis were summarized,including iridoids(64),flavonoids(18),lignin(17),tannins(16),organic acids and phenolic acids(14),triterpenes(11)and others(9),which were further subdivided according to their pharmacological effects.It was found that the pharmacological effects of C.officinalis were very extensive,mainly including antioxidant,anti-inflammatory,anti-tumor,neuroprotective and other biological activities.Among these activities,the anti-inflammatory and hypoglycemic mechanisms showed multi-pathway and multi-target characteristics,which were elaborated systematically.However,there are still many compounds in C.officinalis that have not been studied for biological activity,which means that it still has potential pharmacological activity to be further studied.C.officinalis not only has high medicinal value,but also has a potential edible resource.However,limited by people's understanding of the nutritional value of C.officinalis,few product types and incomplete processing technology,people's acceptance of the edible value of C.oficinalis is not high,and it needs to be further developed and utilized.
基金funded by National Key R&D Program of China(2022)Research on Precision Nutrition and Health Food,Department of Science and Technology of Henan Province(CXJD2021006)Key Project in Science and Technology Agency of Henan Province(212102310355).
文摘Flower plants are popular all over the world and important sources of ornamental plants,bioactive molecules and nutrients.Flowers have a wide range of biological activities and beneficial pharmacological effects.Flowers and their active ingredients are becoming more and more popular in the preparation of food,drugs and industrial products.This paper summarizes the active ingredients,pharmacological activities and applications in the pharmaceutical and food industries of flower plants in recent years.In addition,the possible molecular mechanism of pharmacological effects of flower plants were also discussed.302 active constituents from 55 species of flower plants were summarized,including flavonoids(115),terpenoids(90),phenylpropanoids(20),alkaloids(13),organic acids(27)and others(37).The pharmacological effects of flower plants are very extensive,mainly including antioxidant,anti-inflammatory,anti-tumor,anti-virus,and hypoglycemic.The mechanisms of anti-inflammatory,anti-tumor and hypoglycemic activities present the characteristics of multi-way and multi-target.Because of its rich nutrients,bioactive ingredients and plant essential oils,and its wide sources,flower plants are widely used in food,beverage,cosmetics and drug research.Flower plants also play an important role in pharmaceutical industry,food industry and other fields.
基金funded by National Key R&D Program of China(2022YFF1100300).
文摘Proteomics is a new technology that has been widely applied in the field of life and health science.It effectively addresses issues related to the impact of dietary structure on organs,tissues,and cells,as well as the changes in proteins in various organs,tissues,and cells under disease conditions.The differential proteins identified through proteomics can serve as disease biomarkers and target proteins affecting health and can be used for disease diagnosis and health regulation.In this paper,the application of proteomics in the field of infl ammation in recent years was summarized,especially in the therapeutic target and mechanism of action,which opens up a new way for more effective prevention,diagnosis,and treatment of inflammation,and provides medical protection for human life and health.
基金support from the Grain,Oil,and Food Engineering Technology Research Center of the State Grain and Reserves Administration/Key Laboratory of Henan Province(GO202206)the Cultivation Program for Young Backbone Teachers at Henan University of Technology+3 种基金the Key R&D Projects in Henan Province(231111113300)Double First-Class Discipline Construction Program of Henan University of Technology(0517-24410014)National Key Research and Development Program of China(2023YFF1104600)Joint Research Fund for science and technology R&D Projects of Henan Province(225200810066).
文摘Fusarium graminearum(F.graminearum)is a severe phytopathogen threatening agriculture production and food security.Paeonol,serves as a plant-derived natural component,is a promising antifungal agent.At a concentration of 0.3125 mg/mL,paeonol was adequate to fully inhibit the growth of F.graminearum mycelia within 3 days.Fourier-Transform Infrared Spectroscopy(FT-IR)analysis showed that paeonol had no impact on the outer surface of F.graminearum cell walls.While propidium iodide staining,extracellular conductivity,and pH value measurements demonstrated that paeonol disrupted the cell membrane.Furthermore,lipid oxidation and osmotic stress responses were observed in F.graminearum treated with paeonol,resulting in a 47.23%rise in malondialdehyde(MDA)levels and a 515.43%increase in glycerol levels.Moreover,on the 7th day after exposure to paeonol treatment,the deoxynivalenol(DON)level was significantly reduced,measuring only onefifth of that in the control group.Finally,paeonol was shown to inhibit F.graminearum on wheat grains and steamed bread slices.These results,for the first time,revealed the inhibitory mode of action of paeonol against F.graminearum as reflected by disruption of cell membrane integrity,induction of lipid oxidation and osmotic pressure,as well as DON biosynthesis.Furthermore,this study provided scientific evidence for the potential applications of paeonol in agriculture and food industry.
基金supported by the National Research Centre,Dokki,Cairo,Egypt project#13050302.
文摘Synthesis of zinc oxide nanoparticles(ZnO-NPs)via green method is an outstanding alternative to conventional/regular methods;however,the safety or toxicity of the biosynthesized ZnO-NPs in vivo is not fully explored.This study was conducted to evaluate the protective efficiency of cinnamaldehyde-loaded chitosan nanoparticles(Cin@CSNPs)against oxidative damage and genotoxicity of ZnO-NPs in mice.ZnO-NPs were biosynthesized using the extract of fresh leaves of Mentha pulegium L.Cin was extracted from cinnamon essential oil,and was loaded into chitosan nanoparticle(Cin@CSNPs).Both ZnO-NPs,Cin@CSNPs and CSNPs were characterized.The in vitro release of Cin@CSNPs was determined.In the biological study,6 groups of male BALB/c mice were treated by gavage for 3 weeks as follows,control group,the group received ZnO-NPs(25 mg/kg b.w),the groups received Cin@CSNPs at low dose(50 mg/kg b.w)or high dose(100 mg/kg b.w),and the groups received ZnO-NPs plus Cin@CSNPs at the 2 tested doses.Blood and tissue samples were collected for different biochemical,genetical and histological studies.The particle size of ZnO-NPs,CSNPs,and Cin@CSNPs were(20.78±2.60),(170.0±3.7),and(218.23±2.90)nm,andξ-potential were(32.7±4.6),(8.32±0.27)and(4.80±0.21)mV,respectively.ZnO-NPs disturbed the biochemical and oxidative stress indices,AFP,CEA,TNF-α,chromosomal aberrations in somatic and germ cells,and sperm abnormality along with severe pathological changes in the hepatic,renal,and testicular tissues.Cin@CSNPs improved significantly all the parameters tested and the histological picture in a dose-dependent.Therefore,the biosynthesized ZnO-NPs exhibit oxidative damage and genotoxicity,and Cin@CSNPs have potential protective effects against the risks of ZnO-NPs and may be a promising tool to overcome the challenges of using Cin in food and pharmaceuticals applications.
基金financial support provided by the National Key Research and Development Program of China(2024YFD2101202).
文摘Memory deficits are a significant societal issue.The study sought to examine the specific distribution patterns and the regulatory proteins and pathway mechanisms of Antarctic krill tripeptide Phe-Pro-Phe(FPF)in mitigating scopolamine-induced memory deficits in mice.FPF significantly enhanced behavioral performance,elevated acetylcholine(ACh)levels and unsaturated lipid concentrations,while reducing acetylcholinesterase(AChE)activity and neuronal damage,as evaluated through behavioral studies,Nissl staining,and Fourier transform infrared imaging(FTIR).Additionally,it was shown that FPF might be directed towards the hippocampus.The tissue distribution was as follows:duodenum>jejunum>ileum>hippocampus>kidney,liver,heart,lung,and spleen,employing Liquid Chromatography-Tandem Mass Spectrometry(LC-MS/MS).Proteomics and immunofluorescence analyses indicated that FPF markedly elevated the expression levels of MT-ATP8,SST,and CCS proteins,optimized energy metabolism homeostasis,augmented neural signaling efficiency,and improved synaptic plasticity,thereby exerting a neuroprotective effect and mitigating scopolamine-induced memory deficits.
基金This research was financially supported by Major Public Welfare Projects in Henan Province(201300110200)National Key R&D Program of China(2018YFD0400200)+2 种基金Key scientific and technological key projects of Henan science and Technology Department(192102110214 and 202102110283)Henan Province Industry-University-Research Cooperation Project(182107000033)the special fund project of Zhengzhou basic and applied basic research(ZZSZX202003).
文摘The polysaccharides from edible fungus showed many kinds of biological activities,including anti-tumor,immunomodulatory,anti-inflammatory,anti-diabetes,improving functional constipation activities.In particular,the immunomodulatory effects have been paid more and more attention by scholars,but there was no systematic introduction of their immunomodulatory mechanism.So,this review introduced the immunomodulatory effects and mechanism of edible fungus polysaccharides in recent years,and then the relationships between structure and immunomodulatory effect were also discussed.
基金supported by Research on Precision Nutrition and Health Food,Department of Science and Technology of Henan Province(CXJD2021006)。
文摘Two immunomodulatory polysaccharides(Vp2a-Ⅱ and Vp3) were isolated and identified from Apocynum venetum L. flowers, and their innate immune-stimulating functions and working mechanisms were evaluated in RAW264.7 cells. Both the level of released nitric oxide(NO) and expression of inducible nitric oxide synthase(iNOS) m RNA were significantly enhanced in the RAW264.7 macrophages cells treated by Vp2a-Ⅱ and Vp3. Vp2a-Ⅱ(100–800 μg/m L) and Vp3(400 μg/mL) could significantly increase the phagocytic activity of RAW264.7 cells and the secretion and m RNA expression of TNF-α and IL-6 in a concentrationdependent manner through affecting mitogen-activated protein kinase(MAPK) activity and nuclear factor κB(NF-κB) nuclear translocation. Vp2a-Ⅱ might activate the MAPK signaling pathways and induce the nuclear translocation of NF-κB p65, whilst Vp3 likely activated the NF-κB and MAPK signaling pathways without influencing the p38 MAPK route.
基金This work was funded by the National Key R&D Program of China(2018YFD0400200)Key Project in Science and Technology Agency of Kaifeng City(1906006)Major Public Welfare Projects in Henan Province(201300110200).
文摘This study was designed to elucidate the immunoregulation of Ganoderma lucidum.HPLC fingerprint and spectrum-effect relationship of G.lucidum were established to predict the active compounds and BP neural network model was established to predict the efficacy.Then the target compounds were identified by high resolution mass spectrometry.The results indicated that there are both enhanced immunity and immunosuppressive components in G.lucidum.BP neural network was trained with the common peak area and immune effi cacy index of G.lucidum fi ngerprint as samples,and a combined evaluation system of G.lucidum fi ngerprint effi cacy was established.The correlation coeffi cient R of BP network model was 0.98643,and the error of pharmacodynamic prediction results was in the ideal range.Eight compounds were identifi ed by high resolution mass spectrometry.The compounds related to immune activity in G.lucidum were determined in this study.
基金This research has been financially supported by National Key R&D Program of China(2019YFD1002300)Open Project Program of Grain,Oil and Food Engineering Technology Research Center of the State Grain and Reserves Administration/Key Laboratory of Henan Province,Henan University of Technology(GO202216)+1 种基金Doctor Foundation of Henan University of Technology(2019BS057)Cultivation Programme for Young Backbone Teachers in Henan University of Technology for financial support(21420187).
文摘Chestnut is a high nutritional value food that has been widely used as a tonic in traditional Chinese medicine.As an emerging functional food ingredient,Chinese chestnuts are rich in a range of bioactive nutrients such as starch,dietary fiber,fat,protein,trace metal element and vitamins A,B,C,D and other nutrients.In our study,Chinese chestnut powder(CCP)were added into bread formulation at 2%-6%levels(based on flour weight)to produce fresh bread with enhanced anti-staling characteristics and starch digestion inhibitory ability.The texture properties,retrogradation enthalpy,water distribution,and estimated glycemic index(eGI)of wheat bread containing CCP as a functional additive were also investigated.The results showed that incorporation of CCP apparently affected bread texture,resulting in increased hardness,as well as decreased the specific volume of wheat bread.These influences were generally proportional to the amount of CCP used.It was found that adding too much CCP resulted in a dark red color,showing increased significantly higher total color difference(ΔE)and L values.Conversely,addition of CCP significantly reduced starch digestion rate and digestion extent in bread,and the reduction degree was positively related to the amount of CCP applied.The greatest reduction in eGI value from 79.40(control)to 75.02(6%CPP bread)was observed.Meanwhile,the content of resistant starch of 6%CPP bread was about 1.36 times higher than that of control bread.CCP also reduced crumb water loss and drove the water shift from the bound to the mobile state after stored for 7 days.The retrogradation enthalpy analyses further confirmed that CCP inhibited starch retrogradation and recrystallization.These results suggested that Chinese chestnut powder could be incorporated into fresh bread to provide health functions,such as lowering potential glycaemic response and improving anti-staling characteristics of bread.
基金Supported by National Natural Science Foundation of Hainan Province(217157)Fund for Construction of Hainan Provincial Key Discipline(Thalassochemistry) in 2017
文摘[Objectives] This study was conducted to optimize the determination conditions of amino acids from abalone. [Methods] The sample was treated by acid hydrolysis method and subjected to 2,4-2 nitro fluorobenzene column derivatization. The amino acid content in abalone was determined by HLPC,and the nutritional value of the amino acids was evaluated with egg protein model put forward by Institute of Nutrition and Food Hygiene,Chinese Academy of Preventive Medicine. [Results] Abalone contains full amino acids. According to the FAO/WHO ideal,it is a high-quality protein source and suitable for supplement of protein source for human body. [Conclusions]The experimental method has simple operation and could achieve a good effect with wide linear range and correlation coefficient over 0. 999 8,and the obtained results are satisfactory.
基金funded by National Key R&D Program of China(2022YFF1100300)National Natural Science Foundation of China(31900292)+1 种基金Science and Technology Development Program of Henan Province(212102110469,222102520035)Research on Precision Nutrition and Health Food,Department of Science and Technology of Henan Province(CXJD2021006).
文摘Patrinia scabiosaefolia,is used as wild vegetable in China for more than 2000 years,with a variety of pharmacological activities,including anti-inflammatory,anti-tumor and hypoglycemic.Based on our ongoing research on chemical constituents and hypoglycemic activity of P.scabiosaefolia,4 lignan compounds,(+)-isolariciresinol(1),7R,7’R,8S,8’S-(+)-neo-olivil-4-O-β-D-glucopyranoside(2),4-O-methylcedrusin(3)and patrinian A(4),were isolated and identifi ed.The hypoglycemic activity showed that compounds 2 and 3 could extremely signifi cantly improve insulin resistance at 100(P<0.001),50(P<0.001)and 25μmol/L(P<0.01)in IR 3T3-L1 cells.While compound 4 only promoted glucose uptake by IR 3T3-L1 cells at 100μmol/L(P<0.01).Western blotting experiments showed that compounds 2 and 4 up-regulated the protein expressions of p-IRS,PI-3K,p-AKT and glucose transporter 4(GLUT4),and promoted the transcription of GLUT4 mRNA.Therefore,the mechanisms of compounds 2 and 4 were presumed to improve IR by activating PI-3K/AKT signaling pathway.
基金Yellow River Conservancy Technical Institute(HYJG[2019]7)Innovation Program of Chinese Academy of Sciences(KSCX2-EW-Z-4)Science and technology development plan of Kaifeng in 2020(BKW[2019]95)as well as National Natural Sciences Foundation of China(31101806).
文摘The Escherichia coli(E.coli)is prevailing worldwide,but the epidemiology of E.coli infections feature regional distribution characteristics to some extent.E.coli,as a zoonotic pathogen,can be transferred from animals to humans through food chain or via contact with wounds,causing a public health risk.We reported the swelling of proventriculus and tracheal bleeding following the death in two broiler chickens(Gallus gallus domesticus)from Beijing,China.To investigate whether a virus was involved in the infection,Madin Darby Bovine Kidney(MDCK)cells were co-cultured with supernatants of proventriculus,trachea and spleen homogenates.The avian leucosis virus was detected in the samples of proventriculus and trachea,but the avian influenza virus,the Newcastle disease virus and the avian infectious laryngotracheitis virus were not detected.E.coli isolates were resistant to almost all the antimicrobial as tested except for the combinations of amoxicillin/clavulanic acid and sulfamethoxazole/trimethoprim.PCR tests demonstrated the presence of antibiotic resistance genes in these E.coli isolates and further research revealed a novel gene profile with the presence of CTX-M-1,gyrA,gyrB,oqxA,oqxB,parC and Sul2 antibiotic resistance genes in a strain isolated from a proventriculus sample.These results demonstrated that the presence of antibiotic resistant E.coli would not necessarily cause outbreak of large-scale disease.However,when the bacteria carrying new antibiotic resistance genes enter the environment,it may result in the development of more virulent strains which will potentially impact human and animal health.
基金supported by Key r&D Project of Henan Province(221111110600)research on Precision Nutrition and Health Food,Department of Science and technology of Henan Province(CXJD2021006).
文摘In recent years,Volvariella polysaccharides(VPs)have been confirmed with various of biological activities,which can be used as the main ingredients of functional foods.it was reported to treat various diseases and disorders in humans,and have high nutritional and pharmaceutical significance.this paper summarized the latest progress in the extraction,separation and purification technologies,structure-activity relationship and action mechanisms of VPs,as well as the challenges,limitations during the application that may become the future direction.
基金supported by the National Natural Science Foundation of China(Grant No.32001745)Young Elite Scientists Sponsorship Program by CAST(Grant No.2023QNRC001)+1 种基金the earmarked fund for CARS(Grant No.CARS-03)Food Engineering Technology Research Center/Key Laboratory of Henan Province,Henan University of Tech-nology(Grant No.GO202301).
文摘Fungal contamination and mycotoxin production pose significant challenges to maize preservation.In response,we developed ZnO@SiO2 nanocomposite,with zinc oxide nanoparticles as the core and mesoporous silica encapsulation.Transmission Electron Microscope(TEM)confirmed uniform morphology and good dispersion.Energy Dispersive Spectroscopy(EDS),Fourier Transform Infrared Spectroscopy(FT-IR),and X-Ray Diffraction(XRD)analyses validated the successful fabrication.Assessing the mycelial growth and mycotoxin production of Aspergillus flavus and Fusarium graminearum,two predominant fungi during maize storage,Significant inhibition by the nanocomposites was observed in both maize flour medium and sterile kernels.In maize flour,it sup-pressed A.flavus and F.graminearum toxin production by over 64.11%and 80.53%,respectively.In maize kernels,DON,ZEN,AFB1,AFB2,AFG1,and AFG2 toxins were all inhibited by over 81.3%.Nanocomposite’s effectiveness endured in practical storage,with inhibition rates exceeding 72.03%at an addition concentration of 3.0 g/kg.These findings suggest nanocomposite as a promising tool for mitigating mycotoxin contamination during maize storage,with implications for food safety enhancement.
基金supported by the Food Engineering Technology Research Center/Key Laboratory of Henan Province[grant number GO202309]Key Research and Development Program of Henan Prov-ince[grant number 252102110106]National Natural Science Foundation of China[grant number 31701542].
文摘Soy,peanut and chickpea contain polyphenols that have multiple beneficial biological activities.In this work,two Lactobacillus strains were employed to ferment the three kinds of bean milk and the effects of fermentation on the polyphenols in them were investigated.After fermentation with L.fermentum GD01 and L.plantarum BHP03,the total phenolic contents were increased by 16.90% and 14.64%in soy milk,18.14%and 24.28%in peanut milk,46.39%and 51.91%in chickpea milk,respectively.Specifically,the contents of free phenol,insoluble ester phenol,and insoluble glycosidic phenol increased,while the contents of soluble ester phenol and soluble glycosidic phenol reduced.These changes were positively associated with the antioxidant activities.Further,Lactobacillus fermentation also changed the contents and forms of monomeric phenols in bean milk,with the free monomeric phenols exhibiting the greatest diversity in types.In addition,the fermented bean milk demonstrated a higher release of phenols compared to the unfermented counterparts during simulated digestion,resulting in enhanced antioxidant activities.Notably,the most substantial increase in soluble phenols was detected in fermented chickpea milk(162.66%by GD01,137.48%by BHP03),while the most significant increase in insoluble phenols was observed in fermented peanut milk(147.46%by GD01,150.53%by BHP03).All the results confirmed that Lactobacillus fermentation could alter the structures,compositions,digestive characteristics of the polyphenols in the three kinds of bean milk,ultimately enhancing their antioxidant activities.These results offer a theoretical foundation for the advancement of fermented bean milk products with enhanced biological activity.
基金supported by the Food Engineering Technology Research Center/Key Laboratory of Henan Province[grant number GO202309]Key Research and Development Program of Henan Province[grant number 222102110080]National Natural Science Foun-dation of China[grant number 31701542].
文摘Lactobacillus is the dominant bacterial group in vaginal microbiota,which can inhibit the growth of pathogenic bacteria and maintain the balance of the vaginal microbiota.To develop vaginal probiotics with efficient anti-bacterial effects,in this work,the antibacterial activities of the metabolites from Lactobacillus crispatus BHC04 and L.delbrueckii Q5.4 against three indicator bacteria(Escherichia coli ATCC 8739,Staphylococcus aureus ATCC 25923,Candida albicans CMCC(F)98001)were investigated by determining the growth curves and time-killing curves.And the metabolites of the two strains could completely inhibit the growth of the indicator bacteria.Combined the investigation of confocal laser scanning microscope,flow cytometry and scanning electron mi-croscope,it could be confirmed that the metabolites damaged the cell walls and cell membranes of the indicator bacteria.Metabolites also increased the ROS levels,decreased the fatty acid contents of cell membrane and changed the membrane protein structures in the indicator bacteria as analyzed by FT-IR spectroscopy.Furthermore,the metabolites down-regulated the expression of fatty acid synthesis genes(fabA,fabD,fabG,fabI)in E.coli cell membrane and up-regulated the transcription factors(rpos),which resulted in the synthesis of cyclic fatty acids to cope with the stress of metabolites treatment.All the results revealed that the antibacterial mechanisms of the metabolites were to destroy cellular integrity,disrupt antioxidant system,weaken strength of cell membrane and cause cellular protein misfolding.This work would provide a theoretical support for developing Lactobacillus as functional foods beneficial to gynecological health.
基金supported by the Key Research and Development Project of Henan Province(grant number 251111113400)Cultivation Project of Tuoxin Team in Henan University of Technology(2024TXTD07)+2 种基金Key Research and Development Program of Henan Province[grant number grant number 252102110106]the Food Engineering Technology Research Center/Key Laboratory of Henan Province[grant number GO202309]Henan Province"Double First-Class"Project-Food Science and Technology.
文摘Food allergy(FA)has caused significant impacts on patients'quality of life.This study comprehensively investigated the therapeutic and prophylactic effects of Lacticaseibacillus paracasei BHP06 on an ovalbumin(OVA)-induced allergy model,while elucidating its immunomodulatory mechanisms.The results showed that compared with the model group,mice in high-dose prophylaxis group(PH6 group)exhibited lower clinical scores for allergic symptoms and decreased levels of OVA-specific IgE(OVA-sIgE),OVA-sIgG1,histamine,mouse mast cell protease(mMCP)1,and interleukin(IL)4 by 52.00%,54.43%,18.82%,6.48%,and 29.04%,respectively.Meanwhile,the PH6 group demonstrated significant mitigation of jejunal inflammation,and decrease in the number of mast cells in the intestines of mice.In addition,oral administration of L.paracasei BHP06 maintained immune organ homeostasis in allergic mice,decreased gene expression of IL-4,TNF-α,and GATA-3 in the spleen,increased gene expression of IL-10,T-bet,Foxp3,and TGF-β,and modulated the expression of genes associated with the TLR4/NF-κB signaling pathway.16S rDNA sequencing analysis revealed that the intake of L.paracasei BHP06 enhanced the ratio of Firmicutes/Bacteroidota in the gut,leading to significant alterations in the diversity.In summary,oral administration of L.paracasei BHP06 exhibited multiple beneficial effects in alleviating FA by regulating immune balance,intestinal inflammation,TLR4/NF-κB signaling pathway,and intestinal flora.Additionally,the preventive intervention strategy using L.paracasei BHP06 demonstrates a more comprehensive and stable effect.These findings suggested the potential development of L.paracasei BHP06 as a functional food for the prevention and management of FA.
基金supported by the National Key Research and Devel-opment Program of China(2017YFD0400500)the Graduate Inno-vation Fund of Dalian Polytechnic University.
文摘The peptide Ser-Phe-Gly-Asp-Ile(SFGDI),derived from sea cucumber,has demonstrated neuroprotective effects in cell models;however,the in vivo neuroprotective efficacy of SFGDI remains unexplored.In this study,we utilized a cognitive disorder mouse model induced by scopolamine and observed that daily oral administration of SFGDI at a dosage of 40 mg/kg resulted in improved behavioral performance in the Passive avoidance test,the Channel-type water maze test,and the Novel object recognition test(P<0.05).Furthermore,our investigation into the underlying neuroprotective mechanisms of SFGDI revealed that it reduced oxidative stress levels through the activation of the Sirt3/SOD/ROS pathway,thereby balancing hippocampal unsaturated lipids(P<0.05)in the mouse hippocampus.Moreover,SFGDI significantly decreased hippocampal neuroinflammation by modu-lating the activation of microglia and hypertrophy of astrocytes.Additionally,SFGDI was found to enhance synaptic plasticity in the mouse hippocampus by activating the ChAT/p-CaMKII/BDNF pathway and increasing spine density in neuron cells(P<0.05),particularly mushroom spines(P<0.05).These experiments demon-strate that SFGDI exhibits neuroprotective effects by regulating hippocampal oxidative stress through the Sirt3/SOD/ROS pathway and synaptic plasticity via the ChAT/p-CaMKII/BDNF pathway in the mouse hippocampus,thus emerging as a potential neuro-nutraceutical agent for alleviating memory impairment.
