摘要
The peptide Ser-Phe-Gly-Asp-Ile(SFGDI),derived from sea cucumber,has demonstrated neuroprotective effects in cell models;however,the in vivo neuroprotective efficacy of SFGDI remains unexplored.In this study,we utilized a cognitive disorder mouse model induced by scopolamine and observed that daily oral administration of SFGDI at a dosage of 40 mg/kg resulted in improved behavioral performance in the Passive avoidance test,the Channel-type water maze test,and the Novel object recognition test(P<0.05).Furthermore,our investigation into the underlying neuroprotective mechanisms of SFGDI revealed that it reduced oxidative stress levels through the activation of the Sirt3/SOD/ROS pathway,thereby balancing hippocampal unsaturated lipids(P<0.05)in the mouse hippocampus.Moreover,SFGDI significantly decreased hippocampal neuroinflammation by modu-lating the activation of microglia and hypertrophy of astrocytes.Additionally,SFGDI was found to enhance synaptic plasticity in the mouse hippocampus by activating the ChAT/p-CaMKII/BDNF pathway and increasing spine density in neuron cells(P<0.05),particularly mushroom spines(P<0.05).These experiments demon-strate that SFGDI exhibits neuroprotective effects by regulating hippocampal oxidative stress through the Sirt3/SOD/ROS pathway and synaptic plasticity via the ChAT/p-CaMKII/BDNF pathway in the mouse hippocampus,thus emerging as a potential neuro-nutraceutical agent for alleviating memory impairment.
基金
supported by the National Key Research and Devel-opment Program of China(2017YFD0400500)
the Graduate Inno-vation Fund of Dalian Polytechnic University.
