AIM: To examine the expression of E-cadherin in the primary tumor and to evaluate its relationship with lymph node micrometastasis (LNM).METHODS: The authors studied 850 lymph nodes resected from 30 patients with gast...AIM: To examine the expression of E-cadherin in the primary tumor and to evaluate its relationship with lymph node micrometastasis (LNM).METHODS: The authors studied 850 lymph nodes resected from 30 patients with gastric carcinoma who underwent gastrectomy with lymphadenectomy using reverse transcription polymerase chain reaction (RT-PCR) assay in addition to H&E staining. Cytokeratin-20 (CK-20)gene marker was used in this assay. The level of E-cadherin expression in the primary tumor was examined by immunochemical technique (EliVisionTM plus).RESULTS: LNM was detected in 77 (12.5%) lymph nodes of 14 patients (46.7%) with gastric carcinoma. The incidence of LNM was significantly higher in the diffuse type (12 of 19 cases, 63.2%) than in the intestinal type of gastric carcinoma (2 of 11 cases, 18.2%, P = 0.026). The incidence of LNM also increased in accordance with the depth of tumor invasion. The loss of expression of E cadherin in primary tumors was found in 14 (46.7) of 30 tumors. The absence of E-cadherin expression was significantly associated with the Lauren classification (P = 0.026), lymph node metastasis (P = 0.011), the grade of differentiation (P = 0.004) and the lymphatic invasion (P = 0.001). Expression of E-cadherin was negative in 10 (71.4%) of the 14 patients with LNM, and in 4 (25%) of the 16 patients without LNM (P = 0.026). CONCLUSION: The current results indicate that the RT PCR assay is useful for the detection of LNM and can significantly increase the detection rate of lymph node metastasis in patients with gastric carcinoma. The Laurenclassification and depth of tumor invasion are significantlyassociated with lymph node micrometastases. Our findings also indicate that E-cadherin may play an important role in determining the growth type and differentiation of gastric carcinoma. The loss of E-cadherin expression may contribute to LNM.展开更多
Objective:To establish a rapid,specific and sensitive diagnostic technique for the human Toxoplasma gondii infection in the recipi-ents with allogeneic hematopoietic stem cell transplantation and discuss its clinical ...Objective:To establish a rapid,specific and sensitive diagnostic technique for the human Toxoplasma gondii infection in the recipi-ents with allogeneic hematopoietic stem cell transplantation and discuss its clinical significance.Methods:30 patients undergoing allogeneic hematopoietic stem cell transplantation were detected by using ELISA and PCR.Results:Among 30 recipients undergiong allogeneic hematopoietic stem cell transplantation,3 were positive for Toxoplasma gondiii antigen and 5 for surface antigen p43 gene with the positive rate being 13.3% and 16.67% respectively.20 healthy people(negative for anti-Tox antibody)were also tested by using ELISA and PCR.Conclusion:PCR is an accurate,relatively rapid,sensitive and specific method for detecting P43 gene of Toxoplasma gondii.Be-canuse PCR can be applied to a variety of different clinical samples,it can be considered as a valuable additional tool for identification of Toxoplasma gondii infections.展开更多
The aim of the present study was to confirm observations on the vertical transmission of Schistosoma japonicum in the rabbit. S. japonicum- infected pregnant rabbits were used in this study. Perfusion of mother rabb...The aim of the present study was to confirm observations on the vertical transmission of Schistosoma japonicum in the rabbit. S. japonicum- infected pregnant rabbits were used in this study. Perfusion of mother rabbits was done 9weeks after infection in order to obtain worm bur- dens in relation to their initial cercarial dose.Anti- schistosoma specific Ig M antibodies in serum samples collected from rabbit kittens were detected by EL ISA.Our results showed that gestation period lasted the norm al2 9- 31days.All the exposed mother rabbits became infected with S. japonicum.Positive Ig M antibody OD values were detected in12 out of the6 0 kittens exam ined (2 0 .0 % ) . In group C and A,4 0 .0 % and 17.9% of the kitten were congenitally infected,re- spectively. 18.1% of the kittens born to mothers infected with a single dose of 2 0 0 cercariae per rabbit were positives;this is not significantly different from that obtained for the 6 0 0 dose group (2 2 .2 % ) .Three randomly selected Ig M+ kittens harbored between one and two adult worm s. The livers of these kittens displayed granulomatous lesions. It is concluded that congenital S. japonicum infection does occur in the rabbit and is affected by the m other stage of pregnancy and to a lesser extent by its infection load.展开更多
Objective To investigate the effect and mechanism of radix Sophorae flavescentis on the trophozoites of Giardia lamblia in vitro. Methods Trophozoites of G. lamblia were exposed to aqueous extracts of radix S. flavesc...Objective To investigate the effect and mechanism of radix Sophorae flavescentis on the trophozoites of Giardia lamblia in vitro. Methods Trophozoites of G. lamblia were exposed to aqueous extracts of radix S. flavescentis and then the detachment from the tube wall, the death rate and the morphological changes under light and transmission electron microscope of trophozoites were studied. Results After exposure to 1.6% aqueous extracts of radix S, flarescentis for 6, 8 and 24 h, the detachment rates were 47. 4%, 70. 9% and 80. 2%, and the death rates were 24. 5%,36.1% and 54.2%, respectively. The radix S, flavescentis-treated trophozoites shrinked and became roundish. The rewere also changes in ultrastructure, such as depletion of the contents of cytoplasm, separation of adhesive disk from the cell body and appearance of pit in cell membrane of some treated trophozoites, Conclusion Radix S, flavescentis can kill trophozoites of G, lamblia and lead them to detach from the tube wall in vitro. The induced morphological changes may be partially responsible for the effects of radix S, flavescentis on giardiasis.展开更多
AIM: To obtain the short peptides mimic antigenic epitopes selected by rat natural antibodies to schistosomes, and to explore their immunoprotection against schistosomiasis in mice.METHODS: Adults worm antigens (AWA) ...AIM: To obtain the short peptides mimic antigenic epitopes selected by rat natural antibodies to schistosomes, and to explore their immunoprotection against schistosomiasis in mice.METHODS: Adults worm antigens (AWA) were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked transferred immunoblotting methods with normal SD rat sera (NRS). The killing effects on schistosomula with fresh and heat-inactivated sera from SD rats were observed. Then the purified IgG from sera of SD rats was used to biopan a phage random peptide library and 20 randomly selected positive clones were detected by ELISA and 2 of them were sequenced.Sixty female mice were immunized thrice with positive phage clones (0, 2nd, 4th wk). Each mouse was challenged with 40 cercariae, and all mice were killed 42 d after challenge. The worms and the liver eggs were counted. RESULTS: NRS could specifically react to the molecules of 75 000, 47 000, 34 500 and 23 000 of AWA. Sera from SD rats showed that the mortality rate of schistosomula was 76.2%, and when the sera were heat-inactivated in vitro, the mortality rate was decreased to 41.0% after being cultured for 48 h. The specific phages bound to IgG were enriched about 300-folds after three rounds of biopanning. Twenty clones were detected by ELISA, 19 of them bound to the specific IgG of rat sera. Immunization with these epitopes was carried out in mice. Compared with the control groups, the mixture of two mimic peptides could induce 34.9% (P = 0.000) worm reduction and 67.6% (P = 0.000) total liver egg reduction in mice. Two different mimic peptides could respectively induce 31.0% (P = 0.001), 14.5% (P = 0.074) worm reduction and 61.2% (P = 0.000), 35.7% (P = 0.000) total liver egg reduction. The specific antibody could be induced by immunization of the mimic peptides, and the antibody titer in immunized mice reached more than 1:6 400 as detected by ELISA.CONCLUSION: Specific peptides mimic antigenic molecules can be obtained by biopanning the phage random peptide library and a partially protective immunity against schistosome infection can be stimulated by these phage epitopes in mice.展开更多
Pentastomid parasite Armillifer moniliformis, (A. moniliformis) is a pathogencausing human pentastomiasis. Pentastomiasis is a parasitic zoonosis, which has seldom beenreported. When the number of armillifer parasites...Pentastomid parasite Armillifer moniliformis, (A. moniliformis) is a pathogencausing human pentastomiasis. Pentastomiasis is a parasitic zoonosis, which has seldom beenreported. When the number of armillifer parasites infecting a person is few or very few, theoverwhelming majority of the cases are of asymptomatic or subclinical presentation and are generallydiscovered during autopsies or at surgical operation. A case of A. moniliformis, with heavyinfection caused by nymphal pentastomids and possessing the clinical symptoms of chronic fever andabdominal pain, was admitted to the children 's hospital in Hangzhou, China. As the first case inChina, it is hereby reported.展开更多
Toxoplasmosis, caused by Toxoplasma gondii ( T. gondii ), an obligate intracellular parasite, is a globally distributed zoonosis. It is estimated that one third of population has been infected by this parasite world...Toxoplasmosis, caused by Toxoplasma gondii ( T. gondii ), an obligate intracellular parasite, is a globally distributed zoonosis. It is estimated that one third of population has been infected by this parasite worldwide. In some regions of Europe, the serum antibodies against T. gondii were detected in more than 80% of the examined population. In China, T. gondii was isolated for the first time from a rabbit in Fujian Province in 1954, 1 and the first case of human toxoplasmosis was reported in Jiangxi Province in 1964. 2 Later, in 1978, the first epidemic survey on toxoplasmosis was carried out in Guangxi Province. In 1982, the first national survey on toxoplasmosis supported by the National Natural Sciences Foundation of China was carried out and the result was published in 1988. Since that, many surveys on toxoplasmosis were reported in China. These results indicated that T. gondii infection in this country is very common and in some circumstances, the infection causes serious disease. For instance, T. gondii infection in pregnant women causes abortion, stillbirth, abnormal embryo and thus, it has been considered as the most commonly infectious agent of “TORCH syndrome” (TORCH syndrome refers to infection of a developing fetus or newborn by any of a group of infectious agents. “TORCH” is an acronym meaning toxoplasmosis, other agents, rubella, cytomegalovirus, and herpes simples). In immunocompromised patients, toxoplasmosis is also one of the most frequent complications. Because of the medical importance of T. gondii , more and more Chinese researchers are interested in this parasite. Aetiology Infected animals are the most important source for T. gondii infection, although humans can also be taken as the infective source for this disease particularly the vertical transmission from pregnant woman to fetus through the placenta. T. gondii widely distributes in variant animals from mammals to birds. At least 190 species of animals displayed serologically positive to T. gondii and 141 species of these animals pathogenically infected by T. gondii have been confirmed in China. 3展开更多
The in vivo effects of a single dose of levo-praziquantel,75 mg/kg in PEG 400,on the tegumental surface of adult S.japonicum were compared with the effects of a single dose(150mg/kg) of the mixed isomer preparation,us...The in vivo effects of a single dose of levo-praziquantel,75 mg/kg in PEG 400,on the tegumental surface of adult S.japonicum were compared with the effects of a single dose(150mg/kg) of the mixed isomer preparation,using scanning and transmission electron microscope.Worms were recovered from展开更多
TO construct the recotabinant plasmid —— pLXSN-SjYF to prepare for expression and DNA vaccine of Schistosoma japonicum gene. Methods :Mnpliffing DNA fragment coding yolk fereltin from a female adult Sehistosoma jap...TO construct the recotabinant plasmid —— pLXSN-SjYF to prepare for expression and DNA vaccine of Schistosoma japonicum gene. Methods :Mnpliffing DNA fragment coding yolk fereltin from a female adult Sehistosoma japonicum DNAs by PCR. The fragment was inseted into pLXSN retrovirus vector by digesting with restrictive enzymes and linking reactions. The positive clone was screened on LB plates contmaining amplcillin asld identified by restrictive enzymes digestion and PCR amplification, Resttlts The specific DNA fragmem SjYF was amplified from the female adult SjDNAs. IaLXSN-SjYF was constructed successfully and the further research will be carried out.展开更多
基金Supported by the National Natural Science Foundation of China,No. 30271276
文摘AIM: To examine the expression of E-cadherin in the primary tumor and to evaluate its relationship with lymph node micrometastasis (LNM).METHODS: The authors studied 850 lymph nodes resected from 30 patients with gastric carcinoma who underwent gastrectomy with lymphadenectomy using reverse transcription polymerase chain reaction (RT-PCR) assay in addition to H&E staining. Cytokeratin-20 (CK-20)gene marker was used in this assay. The level of E-cadherin expression in the primary tumor was examined by immunochemical technique (EliVisionTM plus).RESULTS: LNM was detected in 77 (12.5%) lymph nodes of 14 patients (46.7%) with gastric carcinoma. The incidence of LNM was significantly higher in the diffuse type (12 of 19 cases, 63.2%) than in the intestinal type of gastric carcinoma (2 of 11 cases, 18.2%, P = 0.026). The incidence of LNM also increased in accordance with the depth of tumor invasion. The loss of expression of E cadherin in primary tumors was found in 14 (46.7) of 30 tumors. The absence of E-cadherin expression was significantly associated with the Lauren classification (P = 0.026), lymph node metastasis (P = 0.011), the grade of differentiation (P = 0.004) and the lymphatic invasion (P = 0.001). Expression of E-cadherin was negative in 10 (71.4%) of the 14 patients with LNM, and in 4 (25%) of the 16 patients without LNM (P = 0.026). CONCLUSION: The current results indicate that the RT PCR assay is useful for the detection of LNM and can significantly increase the detection rate of lymph node metastasis in patients with gastric carcinoma. The Laurenclassification and depth of tumor invasion are significantlyassociated with lymph node micrometastases. Our findings also indicate that E-cadherin may play an important role in determining the growth type and differentiation of gastric carcinoma. The loss of E-cadherin expression may contribute to LNM.
文摘Objective:To establish a rapid,specific and sensitive diagnostic technique for the human Toxoplasma gondii infection in the recipi-ents with allogeneic hematopoietic stem cell transplantation and discuss its clinical significance.Methods:30 patients undergoing allogeneic hematopoietic stem cell transplantation were detected by using ELISA and PCR.Results:Among 30 recipients undergiong allogeneic hematopoietic stem cell transplantation,3 were positive for Toxoplasma gondiii antigen and 5 for surface antigen p43 gene with the positive rate being 13.3% and 16.67% respectively.20 healthy people(negative for anti-Tox antibody)were also tested by using ELISA and PCR.Conclusion:PCR is an accurate,relatively rapid,sensitive and specific method for detecting P43 gene of Toxoplasma gondii.Be-canuse PCR can be applied to a variety of different clinical samples,it can be considered as a valuable additional tool for identification of Toxoplasma gondii infections.
文摘The aim of the present study was to confirm observations on the vertical transmission of Schistosoma japonicum in the rabbit. S. japonicum- infected pregnant rabbits were used in this study. Perfusion of mother rabbits was done 9weeks after infection in order to obtain worm bur- dens in relation to their initial cercarial dose.Anti- schistosoma specific Ig M antibodies in serum samples collected from rabbit kittens were detected by EL ISA.Our results showed that gestation period lasted the norm al2 9- 31days.All the exposed mother rabbits became infected with S. japonicum.Positive Ig M antibody OD values were detected in12 out of the6 0 kittens exam ined (2 0 .0 % ) . In group C and A,4 0 .0 % and 17.9% of the kitten were congenitally infected,re- spectively. 18.1% of the kittens born to mothers infected with a single dose of 2 0 0 cercariae per rabbit were positives;this is not significantly different from that obtained for the 6 0 0 dose group (2 2 .2 % ) .Three randomly selected Ig M+ kittens harbored between one and two adult worm s. The livers of these kittens displayed granulomatous lesions. It is concluded that congenital S. japonicum infection does occur in the rabbit and is affected by the m other stage of pregnancy and to a lesser extent by its infection load.
文摘Objective To investigate the effect and mechanism of radix Sophorae flavescentis on the trophozoites of Giardia lamblia in vitro. Methods Trophozoites of G. lamblia were exposed to aqueous extracts of radix S. flavescentis and then the detachment from the tube wall, the death rate and the morphological changes under light and transmission electron microscope of trophozoites were studied. Results After exposure to 1.6% aqueous extracts of radix S, flarescentis for 6, 8 and 24 h, the detachment rates were 47. 4%, 70. 9% and 80. 2%, and the death rates were 24. 5%,36.1% and 54.2%, respectively. The radix S, flavescentis-treated trophozoites shrinked and became roundish. The rewere also changes in ultrastructure, such as depletion of the contents of cytoplasm, separation of adhesive disk from the cell body and appearance of pit in cell membrane of some treated trophozoites, Conclusion Radix S, flavescentis can kill trophozoites of G, lamblia and lead them to detach from the tube wall in vitro. The induced morphological changes may be partially responsible for the effects of radix S, flavescentis on giardiasis.
基金Supported by the WHO/TDR, No. 980255 and the Science Commission of Hunan Province, No. 00jzy2115
文摘AIM: To obtain the short peptides mimic antigenic epitopes selected by rat natural antibodies to schistosomes, and to explore their immunoprotection against schistosomiasis in mice.METHODS: Adults worm antigens (AWA) were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked transferred immunoblotting methods with normal SD rat sera (NRS). The killing effects on schistosomula with fresh and heat-inactivated sera from SD rats were observed. Then the purified IgG from sera of SD rats was used to biopan a phage random peptide library and 20 randomly selected positive clones were detected by ELISA and 2 of them were sequenced.Sixty female mice were immunized thrice with positive phage clones (0, 2nd, 4th wk). Each mouse was challenged with 40 cercariae, and all mice were killed 42 d after challenge. The worms and the liver eggs were counted. RESULTS: NRS could specifically react to the molecules of 75 000, 47 000, 34 500 and 23 000 of AWA. Sera from SD rats showed that the mortality rate of schistosomula was 76.2%, and when the sera were heat-inactivated in vitro, the mortality rate was decreased to 41.0% after being cultured for 48 h. The specific phages bound to IgG were enriched about 300-folds after three rounds of biopanning. Twenty clones were detected by ELISA, 19 of them bound to the specific IgG of rat sera. Immunization with these epitopes was carried out in mice. Compared with the control groups, the mixture of two mimic peptides could induce 34.9% (P = 0.000) worm reduction and 67.6% (P = 0.000) total liver egg reduction in mice. Two different mimic peptides could respectively induce 31.0% (P = 0.001), 14.5% (P = 0.074) worm reduction and 61.2% (P = 0.000), 35.7% (P = 0.000) total liver egg reduction. The specific antibody could be induced by immunization of the mimic peptides, and the antibody titer in immunized mice reached more than 1:6 400 as detected by ELISA.CONCLUSION: Specific peptides mimic antigenic molecules can be obtained by biopanning the phage random peptide library and a partially protective immunity against schistosome infection can be stimulated by these phage epitopes in mice.
文摘Pentastomid parasite Armillifer moniliformis, (A. moniliformis) is a pathogencausing human pentastomiasis. Pentastomiasis is a parasitic zoonosis, which has seldom beenreported. When the number of armillifer parasites infecting a person is few or very few, theoverwhelming majority of the cases are of asymptomatic or subclinical presentation and are generallydiscovered during autopsies or at surgical operation. A case of A. moniliformis, with heavyinfection caused by nymphal pentastomids and possessing the clinical symptoms of chronic fever andabdominal pain, was admitted to the children 's hospital in Hangzhou, China. As the first case inChina, it is hereby reported.
文摘Toxoplasmosis, caused by Toxoplasma gondii ( T. gondii ), an obligate intracellular parasite, is a globally distributed zoonosis. It is estimated that one third of population has been infected by this parasite worldwide. In some regions of Europe, the serum antibodies against T. gondii were detected in more than 80% of the examined population. In China, T. gondii was isolated for the first time from a rabbit in Fujian Province in 1954, 1 and the first case of human toxoplasmosis was reported in Jiangxi Province in 1964. 2 Later, in 1978, the first epidemic survey on toxoplasmosis was carried out in Guangxi Province. In 1982, the first national survey on toxoplasmosis supported by the National Natural Sciences Foundation of China was carried out and the result was published in 1988. Since that, many surveys on toxoplasmosis were reported in China. These results indicated that T. gondii infection in this country is very common and in some circumstances, the infection causes serious disease. For instance, T. gondii infection in pregnant women causes abortion, stillbirth, abnormal embryo and thus, it has been considered as the most commonly infectious agent of “TORCH syndrome” (TORCH syndrome refers to infection of a developing fetus or newborn by any of a group of infectious agents. “TORCH” is an acronym meaning toxoplasmosis, other agents, rubella, cytomegalovirus, and herpes simples). In immunocompromised patients, toxoplasmosis is also one of the most frequent complications. Because of the medical importance of T. gondii , more and more Chinese researchers are interested in this parasite. Aetiology Infected animals are the most important source for T. gondii infection, although humans can also be taken as the infective source for this disease particularly the vertical transmission from pregnant woman to fetus through the placenta. T. gondii widely distributes in variant animals from mammals to birds. At least 190 species of animals displayed serologically positive to T. gondii and 141 species of these animals pathogenically infected by T. gondii have been confirmed in China. 3
文摘The in vivo effects of a single dose of levo-praziquantel,75 mg/kg in PEG 400,on the tegumental surface of adult S.japonicum were compared with the effects of a single dose(150mg/kg) of the mixed isomer preparation,using scanning and transmission electron microscope.Worms were recovered from
文摘TO construct the recotabinant plasmid —— pLXSN-SjYF to prepare for expression and DNA vaccine of Schistosoma japonicum gene. Methods :Mnpliffing DNA fragment coding yolk fereltin from a female adult Sehistosoma japonicum DNAs by PCR. The fragment was inseted into pLXSN retrovirus vector by digesting with restrictive enzymes and linking reactions. The positive clone was screened on LB plates contmaining amplcillin asld identified by restrictive enzymes digestion and PCR amplification, Resttlts The specific DNA fragmem SjYF was amplified from the female adult SjDNAs. IaLXSN-SjYF was constructed successfully and the further research will be carried out.
