Objective: To investigate the relationship between the single ALT positive (ELSIA-HBV/HCV negative) and NAT-HBV/HCV on blood donor samples. Methods: 28710 samples were surveyed retrospectively from Dec. 2010 to Dec. 2...Objective: To investigate the relationship between the single ALT positive (ELSIA-HBV/HCV negative) and NAT-HBV/HCV on blood donor samples. Methods: 28710 samples were surveyed retrospectively from Dec. 2010 to Dec. 2011. ALT was detected by rate method, and the single ALT positive samples were detected by ELSIA-HBV/HCV and NAT-HBV/HCV. The relationship between the single ALT positive and NAT-HBV/HCV were analyzed. 21 samples, values in 40 U/L ≤ ALT ≤ 70 U/L, were selected at random from 2516 samples with single ALT positive, which were second detected by ELISA and NAT in the second donation. Results: 2516 (8.74%) single ALT positive samples (ELSIA-HBV/HCV negative) were found in 28710 donors. Among these samples, 8 (2.8/10000) positive were detected by NAT, including 5 HBV-DNA-positive and 3 HCV-RNA-positive. Obviously, positive rate of NAT from the donors whose ALT value ≤ 70 U/L were lower than those of ≥ 71 U/L (P < 0.01). 21 donors were investigated in the second donation in following 153 to 401 days, All samples were negative by ELISA-HBV/HCV and NAT-HBV/HCV. Conclusions: Donors with single ALT positive (value in 40 U/L-70 U/L) are not likely to become HBV/HCV virus carriers or HBV/HCV patients after half or one year. So it is to set ALT abandone threshlod to ≤ 70 U/L can ensure blood safety, and reduce blood abandone in our center.展开更多
Objective: to explore the role of alanine aminotransferase (ALT) primary screening test in the blood testing of Taiyuan Blood Center. Method: from January 2019 to December 2020, Taiyuan Blood Center laboratory of 2 ca...Objective: to explore the role of alanine aminotransferase (ALT) primary screening test in the blood testing of Taiyuan Blood Center. Method: from January 2019 to December 2020, Taiyuan Blood Center laboratory of 2 cases of 236628 blood type samples selected 1200 cases of unpaid blood donation samples as research samples, according to the random number method are divided into group A and B, group A routine screening blood test, group B in group plus alanine aminotransferase (ALT) early screening test. Comparative analysis of the test results between the two groups. Results: the scrap rate of group A blood samples was 0.33% higher than 1.67% in Group B (P <0.05);No laboratory review results and ALT (P> 0.05). Conclusion: the application of alanine aminotransferase (ALT) primary screening test in the blood test has high feasibility and has a significant role in reducing the blood scrap rate, which is worth recommended.展开更多
There are many patients in the blood purification center who need maintenance hemodialysis to maintain life. Those patients generally havelow resistance and are easily exposed to coronavirus because they go back and f...There are many patients in the blood purification center who need maintenance hemodialysis to maintain life. Those patients generally havelow resistance and are easily exposed to coronavirus because they go back and forth the hospital and residence three times a week andclosely contact with family, caregivers, community personnel, people in various means of transportation, medical staff, and other patientsvisiting hospital. Therefore, the blood purification center has become a high‑risk environment for the spread of COVID-19 infection. In viewof this, our center quickly responded to the formulation and implementation of infection prevention and control measures suitable for thecharacteristics of the blood purification center and continuous renal replacement therapy (CRRT) emergency plan for fever and suspectedpatients. According to these measures, we have a positive effect on preventing and controlling nosocomial infection in the blood purificationcenter.展开更多
<strong>Introduction: </strong>In Burkina Faso, screening for hepatitis C virus in blood donations is made using sensitive ELISA (Enzyme Linked Immuno Sorbent Assay) type kits. However, no confirmation of ...<strong>Introduction: </strong>In Burkina Faso, screening for hepatitis C virus in blood donations is made using sensitive ELISA (Enzyme Linked Immuno Sorbent Assay) type kits. However, no confirmation of the positive results obtained with these kits is made before their notification to the blood donors due to the high costs of the confirmation kits of immunoblots type. <strong>Objective:</strong> Evaluate two rapid kits against one immunoblot kit in order to determine the most efficiency which will be proposed as an alternative for the confirmation of ELISA tests in the socio-economic context of Burkina Faso. <strong>Material and Methods: </strong>The study was carried out using a panel of 72 sera, of which 22 were positive for anti-HCV antibodies and 50 were negative. The sera were tested using the Monolisa<span style="white-space:nowrap;">®</span> HCV Ag-Ab ULTRA kit and confirmed with the DECISCAN HCV Plus kit. The panel was then tested with the SD BIOLINE HCV kit and the HCV TRI-DOT kit and the results obtained were evaluated against those of the DECISCAN HCV Plus kit used as “gold standard”. <strong>Results:</strong> Compared to the DECISCAN HCV Plus kit, the HCV TRI-DOT kit exhibited a sensitivity and specificity of 100% and the SD BIOLINE HCV kit a sensitivity of 86.36% and a specificity of 100%. <strong>Conclusion:</strong> Based on the results recorded by the HCV TRI-DOT kit, it would be best suited to the sero-epidemiological context of blood donors from the National Blood Transfusion Center and could be proposed as an alternative for confirmation of ELISA tests.展开更多
Introduction: Despite the progress recorded at the level of transfusion safety in Côte d’Ivoire, much remains to be done, particularly at the level of the medical selection of blood donors. The objective of the ...Introduction: Despite the progress recorded at the level of transfusion safety in Côte d’Ivoire, much remains to be done, particularly at the level of the medical selection of blood donors. The objective of the study was to make an assessment of the pre-donation capillary hemoglobin dosage for the year 2020 of the fixed collection, of the Blood Transfusion Center of Yamoussoukro. Method: This is a retrospective study that took place at the Yamoussoukro Blood Transfusion Center. The data collection related to all old and new blood donors were deemed suitable for the fixed collection of the year 2020. The method chosen for the pre-donation control is that of the portable hemoglobinometer of the HémoCue<sup>®</sup> type, more precisely 201+. Results: Of the 1160 blood donors in the study, the pre-donation hemoglobin level was not measured in 787 (67.8%) subjects of either sex. Of the subjects who had a pre-donation hemoglobin level performed, 97 (26%) blood donors had a sub-standard hemoglobin level, including 15 females and 82 males. Conclusion: Pre-donation hemoglobin testing of blood donors is effective in Yamoussoukro. However, efforts must still be made to improve the quality of the blood collected and to protect blood donors.展开更多
BACKGROUND The concept of“duodenal-type follicular lymphoma(FL)”has already been established as a disease entity.On the other hand,because FL that develops in the colorectum is a rare disease,its clinical characteri...BACKGROUND The concept of“duodenal-type follicular lymphoma(FL)”has already been established as a disease entity.On the other hand,because FL that develops in the colorectum is a rare disease,its clinical characteristics,including its treatment and outcome,are poorly understood.AIM To clarify the characteristic clinical features of colorectal FL.METHODS We identified 5 patients with FL from December 2010 to July 2022 whose colorectal lesions were confirmed by endoscopic biopsy at our hospital and retrospectively examined their clinical features,including endoscopic findings,treatment,and long-term outcomes.RESULTS The median age of the patients was 70 years(range 62-74 years,3 males and 2 females),and 2 patients were considered to have systemic FL lesions.Endoscopic findings revealed elavated lesions in all 5 patients(polypoid 3,flat elavated 1,papular 1).Only 1 patient underwent therapeutic intervention,and 4 patients chose watchful waiting.Except for 1 patient in which the lesion spontaneously regressed,the disease recurred or progressed in 4 patients,3 of whom eventually achieved near complete remission with the administration of rituximab and bendamustine(±polatuzumab vedotin).The 5 patients in this study were still alive or exhibited long-term survival before death;the survival time ranged from 8-29 years after the onset of lymphoma.CONCLUSION In the present study,colorectal FL progressed slowly,and overall survival was good because it was sensitive to anticancer drugs even after the disease progressed without initial therapeutic intervention.展开更多
Objective:To study the application effect of bundle nursing strategy in the maintenance of difficult blood vessels for long-term hemodialysis patients.Methods:A prospective study was conducted on 40 long-term hemodial...Objective:To study the application effect of bundle nursing strategy in the maintenance of difficult blood vessels for long-term hemodialysis patients.Methods:A prospective study was conducted on 40 long-term hemodialysis patients admitted from January 2024 to January 2025.The patients were randomly divided into a control group(20 cases)and an observation group(20 cases)using computer random sampling.The control group received routine nursing for difficult blood vessels,while the observation group received a bundle nursing strategy for difficult blood vessels.The self-care ability,complication rate,and nursing satisfaction were compared between the two groups.Results:After nursing,the self-care ability score of the observation group was higher than that of the control group(P<0.05).The complication rate in the observation group was lower than that in the control group(P<0.05).The nursing satisfaction in the observation group was higher than that in the control group(P<0.05).Conclusion:The application of the bundle nursing strategy in the maintenance of difficult blood vessels for long-term hemodialysis patients is beneficial for improving self-care ability,reducing complications,and increasing patient satisfaction with nursing services.展开更多
BACKGROUND The purpose of this paper is to demonstrate a practical stem cell collection method that provides sufficient stem cells for autologous stem cell transplantation(ASCT)in multiple myeloma(MM)patients despite ...BACKGROUND The purpose of this paper is to demonstrate a practical stem cell collection method that provides sufficient stem cells for autologous stem cell transplantation(ASCT)in multiple myeloma(MM)patients despite low peripheral CD34(pCD34)counts and to describe the benefits of this method for MM patients with limited resources.AIM To demonstrate a practical method for stem cell collection.METHODS Stem cell collection data on the last 300 patients at a community cancer center in Washington were reviewed.We report on the methods of collection,including medi-cations used and timing,used by the blood blank as well as their outcomes.The three MM patients with initially very low pCD34 counts all successfully underwent stem cell collection in a single trip to the transplant center for their ASCT.RESULTS Three patients whose pre-collection pCD34 counts were the lowest and less than 2.5 cells/μL were identified.These patients had the commonality of having multiple barriers to transportation and likely would have been able to make only one trip for the stem cell collections.CONCLUSION Despite particularly low pre-collection peripheral blood CD34 counts,successful autologous stem cell collection in MM patients is feasible by routinely adding plerixafor to granulocyte-colony stimulating factor on day 4 of mobilization.There is limited analysis demonstrating that sufficient stem cells for one or more transplants can be collected using this method.This practical and novel approach may benefit the high number of MM patients who face limited resources,finances,long travel times,and social support.These results are highly relevant to physicians treating similar patients.展开更多
Blood cells are the most integral part of the body,which are made up of erythrocytes,platelets and white blood cells.The examination of subcellular structures and proteins within blood cells at the nanoscale can provi...Blood cells are the most integral part of the body,which are made up of erythrocytes,platelets and white blood cells.The examination of subcellular structures and proteins within blood cells at the nanoscale can provide valuable insights into the health status of an individual,accurate diagnosis,and efficient treatment strategies for diseases.Super-resolution microscopy(SRM)has recently emerged as a cutting-edge tool for the study of blood cells,providing numerous advantages over traditional methods for examining subcellular structures and proteins.In this paper,we focus on outlining the fundamental principles of various SRM techniques and their applications in both normal and diseased states of blood cells.Furthermore,future prospects of SRM techniques in the analysis of blood cells are also discussed.展开更多
BACKGROUND In addition to nodal lesions,over 30%of mantle cell lymphomas(MCLs)also have gastrointestinal involvement,characteristically presenting as multiple lymphomatous polyposis(MLP),which rarely involve the esoph...BACKGROUND In addition to nodal lesions,over 30%of mantle cell lymphomas(MCLs)also have gastrointestinal involvement,characteristically presenting as multiple lymphomatous polyposis(MLP),which rarely involve the esophagus.Most related papers have been case reports,and no comprehensive studies have been conducted;thus,the actual clinical situation has remained unknown for a long time.AIM To elucidate the actual clinical situation of esophageal involvement of MCL presenting with MLP,including its prognosis.METHODS From January 2001 to December 2021,among MCL patients whose gastrointestinal lesions were histopathologically confirmed by endoscopic biopsy at our center,6 patients with MLP in the esophagus were selected.We retro-spectively examined the clinical features of these patients,including their prognosis.RESULTS In all patients,multiple lesions were present in the gastrointestinal tract other than the esophagus and in the lymph nodes throughout the body,and most patients also had lesions involving the bone marrow or spleen.Most of the treatments include chemotherapy,with a 50%survival period of less than 2 years and a 5-year survival rate of approximately 30%,indicating a poor prognosis.CONCLUSION Patients with esophageal involvement of the MCL who presented with MLP had a large tumor burden and poor survival.展开更多
目的研究昆明地区RhD初筛阴性献血者RHD基因的多态性及其分子机制,为建立区域性献血者RHD基因数据库提供数据支持。方法选择昆明地区2023年11月-2024年8月初筛RhD阴性标本218例,采用间接抗球蛋白试验法(IAT)进行RhD阴性确认,采用盐水试...目的研究昆明地区RhD初筛阴性献血者RHD基因的多态性及其分子机制,为建立区域性献血者RHD基因数据库提供数据支持。方法选择昆明地区2023年11月-2024年8月初筛RhD阴性标本218例,采用间接抗球蛋白试验法(IAT)进行RhD阴性确认,采用盐水试管法进行RhCE表型鉴定。提取全血基因组DNA,采用PCR-SSP法/SSP荧光PCR染料法进行RHD基因分型,对无法确定基因型的标本进行RHD基因1~10外显子Sanger测序分析。结果检出RhD真阴性表型179例(82.11%),其中RHD*01N.01(RHD全缺失)型154例(86.03%),表型以ccee为主(87.01%);携带非功能性RHD等位基因25例(13.97%),包括RHD*01N.0320例、RHD*01N.163例、RHD*01N.051例、RHD*01N.591例,表型以Ccee为主(64%)。检出D变异型39例(15.89%),其中RHD*DEL1(c.1227G>A)型34例,表型均为C抗原阳性(Ccee 27例,CCee 7例);弱D/部分D型4例,包括RHD*DVI.32例、RHD*weak D type 711例、RHD*weak D type1081例;另检出1例RHD*01/RHD*01N.01,基因型与血清学表型结果不一致。RHD*01N.01女性献血者不规则抗体(主要为抗-D)阳性率9.84%。结论昆明地区RhD初筛阴性献血者RHD基因多态性显著,RhD真阴性比例高于国内部分地区,D变异型以“亚洲型”DEL为主,比例低于国内部分地区。研究结果为本地区RhD阴性和D变异型个体精准输血提供了理论和数据支持。展开更多
文摘Objective: To investigate the relationship between the single ALT positive (ELSIA-HBV/HCV negative) and NAT-HBV/HCV on blood donor samples. Methods: 28710 samples were surveyed retrospectively from Dec. 2010 to Dec. 2011. ALT was detected by rate method, and the single ALT positive samples were detected by ELSIA-HBV/HCV and NAT-HBV/HCV. The relationship between the single ALT positive and NAT-HBV/HCV were analyzed. 21 samples, values in 40 U/L ≤ ALT ≤ 70 U/L, were selected at random from 2516 samples with single ALT positive, which were second detected by ELISA and NAT in the second donation. Results: 2516 (8.74%) single ALT positive samples (ELSIA-HBV/HCV negative) were found in 28710 donors. Among these samples, 8 (2.8/10000) positive were detected by NAT, including 5 HBV-DNA-positive and 3 HCV-RNA-positive. Obviously, positive rate of NAT from the donors whose ALT value ≤ 70 U/L were lower than those of ≥ 71 U/L (P < 0.01). 21 donors were investigated in the second donation in following 153 to 401 days, All samples were negative by ELISA-HBV/HCV and NAT-HBV/HCV. Conclusions: Donors with single ALT positive (value in 40 U/L-70 U/L) are not likely to become HBV/HCV virus carriers or HBV/HCV patients after half or one year. So it is to set ALT abandone threshlod to ≤ 70 U/L can ensure blood safety, and reduce blood abandone in our center.
文摘Objective: to explore the role of alanine aminotransferase (ALT) primary screening test in the blood testing of Taiyuan Blood Center. Method: from January 2019 to December 2020, Taiyuan Blood Center laboratory of 2 cases of 236628 blood type samples selected 1200 cases of unpaid blood donation samples as research samples, according to the random number method are divided into group A and B, group A routine screening blood test, group B in group plus alanine aminotransferase (ALT) early screening test. Comparative analysis of the test results between the two groups. Results: the scrap rate of group A blood samples was 0.33% higher than 1.67% in Group B (P <0.05);No laboratory review results and ALT (P> 0.05). Conclusion: the application of alanine aminotransferase (ALT) primary screening test in the blood test has high feasibility and has a significant role in reducing the blood scrap rate, which is worth recommended.
文摘There are many patients in the blood purification center who need maintenance hemodialysis to maintain life. Those patients generally havelow resistance and are easily exposed to coronavirus because they go back and forth the hospital and residence three times a week andclosely contact with family, caregivers, community personnel, people in various means of transportation, medical staff, and other patientsvisiting hospital. Therefore, the blood purification center has become a high‑risk environment for the spread of COVID-19 infection. In viewof this, our center quickly responded to the formulation and implementation of infection prevention and control measures suitable for thecharacteristics of the blood purification center and continuous renal replacement therapy (CRRT) emergency plan for fever and suspectedpatients. According to these measures, we have a positive effect on preventing and controlling nosocomial infection in the blood purificationcenter.
文摘<strong>Introduction: </strong>In Burkina Faso, screening for hepatitis C virus in blood donations is made using sensitive ELISA (Enzyme Linked Immuno Sorbent Assay) type kits. However, no confirmation of the positive results obtained with these kits is made before their notification to the blood donors due to the high costs of the confirmation kits of immunoblots type. <strong>Objective:</strong> Evaluate two rapid kits against one immunoblot kit in order to determine the most efficiency which will be proposed as an alternative for the confirmation of ELISA tests in the socio-economic context of Burkina Faso. <strong>Material and Methods: </strong>The study was carried out using a panel of 72 sera, of which 22 were positive for anti-HCV antibodies and 50 were negative. The sera were tested using the Monolisa<span style="white-space:nowrap;">®</span> HCV Ag-Ab ULTRA kit and confirmed with the DECISCAN HCV Plus kit. The panel was then tested with the SD BIOLINE HCV kit and the HCV TRI-DOT kit and the results obtained were evaluated against those of the DECISCAN HCV Plus kit used as “gold standard”. <strong>Results:</strong> Compared to the DECISCAN HCV Plus kit, the HCV TRI-DOT kit exhibited a sensitivity and specificity of 100% and the SD BIOLINE HCV kit a sensitivity of 86.36% and a specificity of 100%. <strong>Conclusion:</strong> Based on the results recorded by the HCV TRI-DOT kit, it would be best suited to the sero-epidemiological context of blood donors from the National Blood Transfusion Center and could be proposed as an alternative for confirmation of ELISA tests.
文摘Introduction: Despite the progress recorded at the level of transfusion safety in Côte d’Ivoire, much remains to be done, particularly at the level of the medical selection of blood donors. The objective of the study was to make an assessment of the pre-donation capillary hemoglobin dosage for the year 2020 of the fixed collection, of the Blood Transfusion Center of Yamoussoukro. Method: This is a retrospective study that took place at the Yamoussoukro Blood Transfusion Center. The data collection related to all old and new blood donors were deemed suitable for the fixed collection of the year 2020. The method chosen for the pre-donation control is that of the portable hemoglobinometer of the HémoCue<sup>®</sup> type, more precisely 201+. Results: Of the 1160 blood donors in the study, the pre-donation hemoglobin level was not measured in 787 (67.8%) subjects of either sex. Of the subjects who had a pre-donation hemoglobin level performed, 97 (26%) blood donors had a sub-standard hemoglobin level, including 15 females and 82 males. Conclusion: Pre-donation hemoglobin testing of blood donors is effective in Yamoussoukro. However, efforts must still be made to improve the quality of the blood collected and to protect blood donors.
文摘BACKGROUND The concept of“duodenal-type follicular lymphoma(FL)”has already been established as a disease entity.On the other hand,because FL that develops in the colorectum is a rare disease,its clinical characteristics,including its treatment and outcome,are poorly understood.AIM To clarify the characteristic clinical features of colorectal FL.METHODS We identified 5 patients with FL from December 2010 to July 2022 whose colorectal lesions were confirmed by endoscopic biopsy at our hospital and retrospectively examined their clinical features,including endoscopic findings,treatment,and long-term outcomes.RESULTS The median age of the patients was 70 years(range 62-74 years,3 males and 2 females),and 2 patients were considered to have systemic FL lesions.Endoscopic findings revealed elavated lesions in all 5 patients(polypoid 3,flat elavated 1,papular 1).Only 1 patient underwent therapeutic intervention,and 4 patients chose watchful waiting.Except for 1 patient in which the lesion spontaneously regressed,the disease recurred or progressed in 4 patients,3 of whom eventually achieved near complete remission with the administration of rituximab and bendamustine(±polatuzumab vedotin).The 5 patients in this study were still alive or exhibited long-term survival before death;the survival time ranged from 8-29 years after the onset of lymphoma.CONCLUSION In the present study,colorectal FL progressed slowly,and overall survival was good because it was sensitive to anticancer drugs even after the disease progressed without initial therapeutic intervention.
文摘Objective:To study the application effect of bundle nursing strategy in the maintenance of difficult blood vessels for long-term hemodialysis patients.Methods:A prospective study was conducted on 40 long-term hemodialysis patients admitted from January 2024 to January 2025.The patients were randomly divided into a control group(20 cases)and an observation group(20 cases)using computer random sampling.The control group received routine nursing for difficult blood vessels,while the observation group received a bundle nursing strategy for difficult blood vessels.The self-care ability,complication rate,and nursing satisfaction were compared between the two groups.Results:After nursing,the self-care ability score of the observation group was higher than that of the control group(P<0.05).The complication rate in the observation group was lower than that in the control group(P<0.05).The nursing satisfaction in the observation group was higher than that in the control group(P<0.05).Conclusion:The application of the bundle nursing strategy in the maintenance of difficult blood vessels for long-term hemodialysis patients is beneficial for improving self-care ability,reducing complications,and increasing patient satisfaction with nursing services.
文摘BACKGROUND The purpose of this paper is to demonstrate a practical stem cell collection method that provides sufficient stem cells for autologous stem cell transplantation(ASCT)in multiple myeloma(MM)patients despite low peripheral CD34(pCD34)counts and to describe the benefits of this method for MM patients with limited resources.AIM To demonstrate a practical method for stem cell collection.METHODS Stem cell collection data on the last 300 patients at a community cancer center in Washington were reviewed.We report on the methods of collection,including medi-cations used and timing,used by the blood blank as well as their outcomes.The three MM patients with initially very low pCD34 counts all successfully underwent stem cell collection in a single trip to the transplant center for their ASCT.RESULTS Three patients whose pre-collection pCD34 counts were the lowest and less than 2.5 cells/μL were identified.These patients had the commonality of having multiple barriers to transportation and likely would have been able to make only one trip for the stem cell collections.CONCLUSION Despite particularly low pre-collection peripheral blood CD34 counts,successful autologous stem cell collection in MM patients is feasible by routinely adding plerixafor to granulocyte-colony stimulating factor on day 4 of mobilization.There is limited analysis demonstrating that sufficient stem cells for one or more transplants can be collected using this method.This practical and novel approach may benefit the high number of MM patients who face limited resources,finances,long travel times,and social support.These results are highly relevant to physicians treating similar patients.
基金supported by the following grants:National Key R&D Program of China(Grant no.2022YFC3401100)National Natural Science Foundation of China(Grant nos.32271428,92054110,32201132 and 31600692).
文摘Blood cells are the most integral part of the body,which are made up of erythrocytes,platelets and white blood cells.The examination of subcellular structures and proteins within blood cells at the nanoscale can provide valuable insights into the health status of an individual,accurate diagnosis,and efficient treatment strategies for diseases.Super-resolution microscopy(SRM)has recently emerged as a cutting-edge tool for the study of blood cells,providing numerous advantages over traditional methods for examining subcellular structures and proteins.In this paper,we focus on outlining the fundamental principles of various SRM techniques and their applications in both normal and diseased states of blood cells.Furthermore,future prospects of SRM techniques in the analysis of blood cells are also discussed.
文摘BACKGROUND In addition to nodal lesions,over 30%of mantle cell lymphomas(MCLs)also have gastrointestinal involvement,characteristically presenting as multiple lymphomatous polyposis(MLP),which rarely involve the esophagus.Most related papers have been case reports,and no comprehensive studies have been conducted;thus,the actual clinical situation has remained unknown for a long time.AIM To elucidate the actual clinical situation of esophageal involvement of MCL presenting with MLP,including its prognosis.METHODS From January 2001 to December 2021,among MCL patients whose gastrointestinal lesions were histopathologically confirmed by endoscopic biopsy at our center,6 patients with MLP in the esophagus were selected.We retro-spectively examined the clinical features of these patients,including their prognosis.RESULTS In all patients,multiple lesions were present in the gastrointestinal tract other than the esophagus and in the lymph nodes throughout the body,and most patients also had lesions involving the bone marrow or spleen.Most of the treatments include chemotherapy,with a 50%survival period of less than 2 years and a 5-year survival rate of approximately 30%,indicating a poor prognosis.CONCLUSION Patients with esophageal involvement of the MCL who presented with MLP had a large tumor burden and poor survival.
文摘目的研究昆明地区RhD初筛阴性献血者RHD基因的多态性及其分子机制,为建立区域性献血者RHD基因数据库提供数据支持。方法选择昆明地区2023年11月-2024年8月初筛RhD阴性标本218例,采用间接抗球蛋白试验法(IAT)进行RhD阴性确认,采用盐水试管法进行RhCE表型鉴定。提取全血基因组DNA,采用PCR-SSP法/SSP荧光PCR染料法进行RHD基因分型,对无法确定基因型的标本进行RHD基因1~10外显子Sanger测序分析。结果检出RhD真阴性表型179例(82.11%),其中RHD*01N.01(RHD全缺失)型154例(86.03%),表型以ccee为主(87.01%);携带非功能性RHD等位基因25例(13.97%),包括RHD*01N.0320例、RHD*01N.163例、RHD*01N.051例、RHD*01N.591例,表型以Ccee为主(64%)。检出D变异型39例(15.89%),其中RHD*DEL1(c.1227G>A)型34例,表型均为C抗原阳性(Ccee 27例,CCee 7例);弱D/部分D型4例,包括RHD*DVI.32例、RHD*weak D type 711例、RHD*weak D type1081例;另检出1例RHD*01/RHD*01N.01,基因型与血清学表型结果不一致。RHD*01N.01女性献血者不规则抗体(主要为抗-D)阳性率9.84%。结论昆明地区RhD初筛阴性献血者RHD基因多态性显著,RhD真阴性比例高于国内部分地区,D变异型以“亚洲型”DEL为主,比例低于国内部分地区。研究结果为本地区RhD阴性和D变异型个体精准输血提供了理论和数据支持。
