Animal models are crucial for the study of severe infectious diseases,which is essential for determining their pathogenesis and the development of vaccines and drugs.Animal experiments involving risk grade 3 agents su...Animal models are crucial for the study of severe infectious diseases,which is essential for determining their pathogenesis and the development of vaccines and drugs.Animal experiments involving risk grade 3 agents such as SARS CoV,HIV,M.tb,H7N9,and Brucella must be conducted in an Animal Biosafety Level 3(ABSL-3)facility.Because of the in vivo work,the biosafety risk in ABSL-3 facilities is higher than that in BSL-3 facilities.Undoubtedly,management practices must be strengthened to ensure biosafety in the ABSL-3 facility.Meanwhile,we cannot ignore the reliable scientific results obtained from animal experiments conducted in ABSL-3 laboratories.It is of great practical significance to study the overall biosafety concepts that can increase the scientific data quality.Based on the management of animal experiments in the ABSL-3 Laboratory of Wuhan University,combined with relevant international and domestic literature,we indicate the main safety issues and factors affecting animal experiment results at ABSL-3 facilities.Based on these issues,management practices regarding animal experiments in ABSL-3 facilities are proposed,which take into account both biosafety and scientifically sound data.展开更多
基金We are grateful for the funding from the National Key Research and Development Program of China(grant No.:2016YFC1202203).
文摘Animal models are crucial for the study of severe infectious diseases,which is essential for determining their pathogenesis and the development of vaccines and drugs.Animal experiments involving risk grade 3 agents such as SARS CoV,HIV,M.tb,H7N9,and Brucella must be conducted in an Animal Biosafety Level 3(ABSL-3)facility.Because of the in vivo work,the biosafety risk in ABSL-3 facilities is higher than that in BSL-3 facilities.Undoubtedly,management practices must be strengthened to ensure biosafety in the ABSL-3 facility.Meanwhile,we cannot ignore the reliable scientific results obtained from animal experiments conducted in ABSL-3 laboratories.It is of great practical significance to study the overall biosafety concepts that can increase the scientific data quality.Based on the management of animal experiments in the ABSL-3 Laboratory of Wuhan University,combined with relevant international and domestic literature,we indicate the main safety issues and factors affecting animal experiment results at ABSL-3 facilities.Based on these issues,management practices regarding animal experiments in ABSL-3 facilities are proposed,which take into account both biosafety and scientifically sound data.
