Based on the data from the survey of cashmere-goat and wool-sheep in 12 demonstration counties of China's main producing areas in 2012,the development of farming professional cooperatives of cashmere-goat and wool...Based on the data from the survey of cashmere-goat and wool-sheep in 12 demonstration counties of China's main producing areas in 2012,the development of farming professional cooperatives of cashmere-goat and wool-sheep was analyzed from aspects of status quo,problems and causes,and some suggestions about cooperation cognition and mode,service capacity,personnel training,system improvement and preferential policies were put forward to promote the development of the farming professional cooperatives.展开更多
为了筛选影响发情卵巢发育的关键蛋白,解析彭波半细毛羊繁殖的调控机制,该研究采用TMT(tandem mass tags)蛋白质组学技术对不同发情状态彭波半细毛羊的卵巢进行蛋白质组测序,通过比较分析彭波半细毛羊发情和乏情卵巢蛋白组数据共筛选出...为了筛选影响发情卵巢发育的关键蛋白,解析彭波半细毛羊繁殖的调控机制,该研究采用TMT(tandem mass tags)蛋白质组学技术对不同发情状态彭波半细毛羊的卵巢进行蛋白质组测序,通过比较分析彭波半细毛羊发情和乏情卵巢蛋白组数据共筛选出307个差异表达蛋白,其中下调的差异蛋白为249个(P<0.05),上调的差异蛋白为58个(P<0.05)。这些差异蛋白涉及多种GO分类及KEGG通路。其中GO分类注释显示,差异蛋白与谷胱甘肽转移酶活性,酰基辅酶A脱氢酶活性和磷脂酰肌醇结合等分子功能通路存在密切关联。KEGG通路分析显示,剪切体通路富集水平最高,其中包括SF3A2、PRPF19、BUD31等剪切体通路上的蛋白在细胞生长和分化、凋亡等生物过程中发挥重要作用。同时也发现补体和凝血级联、谷胱甘肽代谢以及细胞色素P450相关通路也表现出显著富集。这些结果为进一步阐明彭波半细毛羊发情和乏情卵巢组织的生理功能变化提供了基础,同时也为全面了解彭波半细毛羊发情规律提供新的参考。展开更多
基金Supported by the Project of the Ministry of Agriculture and the Ministry of Finance(CARS-40-20)
文摘Based on the data from the survey of cashmere-goat and wool-sheep in 12 demonstration counties of China's main producing areas in 2012,the development of farming professional cooperatives of cashmere-goat and wool-sheep was analyzed from aspects of status quo,problems and causes,and some suggestions about cooperation cognition and mode,service capacity,personnel training,system improvement and preferential policies were put forward to promote the development of the farming professional cooperatives.
文摘为了筛选影响发情卵巢发育的关键蛋白,解析彭波半细毛羊繁殖的调控机制,该研究采用TMT(tandem mass tags)蛋白质组学技术对不同发情状态彭波半细毛羊的卵巢进行蛋白质组测序,通过比较分析彭波半细毛羊发情和乏情卵巢蛋白组数据共筛选出307个差异表达蛋白,其中下调的差异蛋白为249个(P<0.05),上调的差异蛋白为58个(P<0.05)。这些差异蛋白涉及多种GO分类及KEGG通路。其中GO分类注释显示,差异蛋白与谷胱甘肽转移酶活性,酰基辅酶A脱氢酶活性和磷脂酰肌醇结合等分子功能通路存在密切关联。KEGG通路分析显示,剪切体通路富集水平最高,其中包括SF3A2、PRPF19、BUD31等剪切体通路上的蛋白在细胞生长和分化、凋亡等生物过程中发挥重要作用。同时也发现补体和凝血级联、谷胱甘肽代谢以及细胞色素P450相关通路也表现出显著富集。这些结果为进一步阐明彭波半细毛羊发情和乏情卵巢组织的生理功能变化提供了基础,同时也为全面了解彭波半细毛羊发情规律提供新的参考。
