利用传统培养法和变性梯度凝胶电泳技术(PCR-DGGE)评价Lactobacillus plantarum WCFS1对小鼠肠道微生物数量和种类的影响。结果表明,乳酸杆菌数量在灌胃期和恢复期较空白期显著升高(P<0.05),而肠杆菌数量在灌胃期和恢复期较空白期显...利用传统培养法和变性梯度凝胶电泳技术(PCR-DGGE)评价Lactobacillus plantarum WCFS1对小鼠肠道微生物数量和种类的影响。结果表明,乳酸杆菌数量在灌胃期和恢复期较空白期显著升高(P<0.05),而肠杆菌数量在灌胃期和恢复期较空白期显著下降(P<0.01);DGGE图谱及多样性分析显示小鼠灌胃期间菌群多样性显著提高(P<0.01),但在停止灌胃7d后与空白期相比无显著性差异(P>0.05)。此外,DGGE条带测序表明Lacto-bacillus plantarum WCFS1在灌胃期间可在大部分小鼠肠道内占据优势地位。展开更多
Chia seed mucilage was probed for the presence of structurally diverse free oligosaccharides along with the assessment of hydrolytic and transgalactosylation activities of α-galactosidase from a probiotic bacterium-L...Chia seed mucilage was probed for the presence of structurally diverse free oligosaccharides along with the assessment of hydrolytic and transgalactosylation activities of α-galactosidase from a probiotic bacterium-Lactiplantibacillus plantarum WCFS1(LpαG)on isolated oligosaccharides.Porous graphitic carbon-liquid chromatography-tandem mass spectrometry(PGC LC-MS/MS)analysis revealed the presence of mainly sucrose(disaccharide),planteose(trisaccharide),few isomeric tetrasaccharides as well as lower abundance of isomeric tri-,tetra-,penta-and hexasaccharides.In vitro evaluation of the digestibility of planteose and tetrasaccharides to the intestinal enzymes did not generate monosaccharides.However,LpαG displayed hydrolytic activity towards planteose and selectively acted on two tetrasaccharides,which were identified as sesamose and stachyose.In addition,transglycosylation property of LpαG using planteose led to the synthesis of sesamose.Thus,the hydrolysis of planteose and sesamose was demonstrated for the first time for anα-galactosidase from the probiotic bacterium.Furthermore,the oligosaccharides pool was shown to influence the growth of probiotic L.acidophilus,L.brevis and L.plantarum in vitro,along with the production of short chain fatty acids(SCFAs).These findings would contribute to the potential application of chia seeds to further enhance its health benefits.展开更多
基于进程通信的电力分布式计算模型满足了电力企业利用电力分布式计算理论对电网安全经济运行情况做出实时监测和分析计算的实际需求,但存在公有基础功能开发复杂性高、系统可扩展性不足等问题。设计并实现了一种基于面向服务架构的电...基于进程通信的电力分布式计算模型满足了电力企业利用电力分布式计算理论对电网安全经济运行情况做出实时监测和分析计算的实际需求,但存在公有基础功能开发复杂性高、系统可扩展性不足等问题。设计并实现了一种基于面向服务架构的电力分布式计算系统模型。运用软件设计模式设计了低耦合、可扩展的系统框架,设计使用Windows通信基础(Windows Communication Foundation,WCF)技术实现面向服务的分布式数据通信,使用Windows工作流基础(Windows Workflow Foundation,WWF)技术可视化地实现电力分布式计算中的各种业务逻辑和计算流程,并结合ADO.NET数据库访问技术实现异构数据的集成。面向服务的系统模型架构提高了系统的可集成性和可扩展性,WCF和WWF技术的使用降低了分布式计算系统开发的复杂性。展开更多
结合面向服务架构(SOA)的技术特点及设备智能维护系统的应用需求,提出基于面向服务的设备智能维护系统的体系结构,该架构与传统软件架构相比具有松耦合、可扩展、服务的柔性组合等诸多优点。在.NET平台用面向服务的分布式编程模型WCF(Wi...结合面向服务架构(SOA)的技术特点及设备智能维护系统的应用需求,提出基于面向服务的设备智能维护系统的体系结构,该架构与传统软件架构相比具有松耦合、可扩展、服务的柔性组合等诸多优点。在.NET平台用面向服务的分布式编程模型WCF(Windows Communication Foundation)进行系统开发,用实际项目验证了该体系的有效性,指出SOA在开发设备智能维护系统的开发与应用中的重要价值。展开更多
为了有效管理农机资源,实时监控农机作业状态和工况数据,及时对农机故障进行预警,提高作业效率,采用.NET提供的应用程序开发接口服务(WCF)生成Web Service客户端,并在此基础上通过ArcGIS Server Java框架进行二次开发,设计并实现了基于A...为了有效管理农机资源,实时监控农机作业状态和工况数据,及时对农机故障进行预警,提高作业效率,采用.NET提供的应用程序开发接口服务(WCF)生成Web Service客户端,并在此基础上通过ArcGIS Server Java框架进行二次开发,设计并实现了基于ArcGIS Server的农机远程监管服务系统。该系统的主要功能包括农机信息管理、合作社内部信息管理、农机实时监控、农机历史轨迹查询、农机工况数据实时监测与即时报警。展开更多
文摘利用传统培养法和变性梯度凝胶电泳技术(PCR-DGGE)评价Lactobacillus plantarum WCFS1对小鼠肠道微生物数量和种类的影响。结果表明,乳酸杆菌数量在灌胃期和恢复期较空白期显著升高(P<0.05),而肠杆菌数量在灌胃期和恢复期较空白期显著下降(P<0.01);DGGE图谱及多样性分析显示小鼠灌胃期间菌群多样性显著提高(P<0.01),但在停止灌胃7d后与空白期相比无显著性差异(P>0.05)。此外,DGGE条带测序表明Lacto-bacillus plantarum WCFS1在灌胃期间可在大部分小鼠肠道内占据优势地位。
文摘Chia seed mucilage was probed for the presence of structurally diverse free oligosaccharides along with the assessment of hydrolytic and transgalactosylation activities of α-galactosidase from a probiotic bacterium-Lactiplantibacillus plantarum WCFS1(LpαG)on isolated oligosaccharides.Porous graphitic carbon-liquid chromatography-tandem mass spectrometry(PGC LC-MS/MS)analysis revealed the presence of mainly sucrose(disaccharide),planteose(trisaccharide),few isomeric tetrasaccharides as well as lower abundance of isomeric tri-,tetra-,penta-and hexasaccharides.In vitro evaluation of the digestibility of planteose and tetrasaccharides to the intestinal enzymes did not generate monosaccharides.However,LpαG displayed hydrolytic activity towards planteose and selectively acted on two tetrasaccharides,which were identified as sesamose and stachyose.In addition,transglycosylation property of LpαG using planteose led to the synthesis of sesamose.Thus,the hydrolysis of planteose and sesamose was demonstrated for the first time for anα-galactosidase from the probiotic bacterium.Furthermore,the oligosaccharides pool was shown to influence the growth of probiotic L.acidophilus,L.brevis and L.plantarum in vitro,along with the production of short chain fatty acids(SCFAs).These findings would contribute to the potential application of chia seeds to further enhance its health benefits.
文摘基于进程通信的电力分布式计算模型满足了电力企业利用电力分布式计算理论对电网安全经济运行情况做出实时监测和分析计算的实际需求,但存在公有基础功能开发复杂性高、系统可扩展性不足等问题。设计并实现了一种基于面向服务架构的电力分布式计算系统模型。运用软件设计模式设计了低耦合、可扩展的系统框架,设计使用Windows通信基础(Windows Communication Foundation,WCF)技术实现面向服务的分布式数据通信,使用Windows工作流基础(Windows Workflow Foundation,WWF)技术可视化地实现电力分布式计算中的各种业务逻辑和计算流程,并结合ADO.NET数据库访问技术实现异构数据的集成。面向服务的系统模型架构提高了系统的可集成性和可扩展性,WCF和WWF技术的使用降低了分布式计算系统开发的复杂性。
文摘结合面向服务架构(SOA)的技术特点及设备智能维护系统的应用需求,提出基于面向服务的设备智能维护系统的体系结构,该架构与传统软件架构相比具有松耦合、可扩展、服务的柔性组合等诸多优点。在.NET平台用面向服务的分布式编程模型WCF(Windows Communication Foundation)进行系统开发,用实际项目验证了该体系的有效性,指出SOA在开发设备智能维护系统的开发与应用中的重要价值。
文摘为了有效管理农机资源,实时监控农机作业状态和工况数据,及时对农机故障进行预警,提高作业效率,采用.NET提供的应用程序开发接口服务(WCF)生成Web Service客户端,并在此基础上通过ArcGIS Server Java框架进行二次开发,设计并实现了基于ArcGIS Server的农机远程监管服务系统。该系统的主要功能包括农机信息管理、合作社内部信息管理、农机实时监控、农机历史轨迹查询、农机工况数据实时监测与即时报警。
