摘要
利用传统培养法和变性梯度凝胶电泳技术(PCR-DGGE)评价Lactobacillus plantarum WCFS1对小鼠肠道微生物数量和种类的影响。结果表明,乳酸杆菌数量在灌胃期和恢复期较空白期显著升高(P<0.05),而肠杆菌数量在灌胃期和恢复期较空白期显著下降(P<0.01);DGGE图谱及多样性分析显示小鼠灌胃期间菌群多样性显著提高(P<0.01),但在停止灌胃7d后与空白期相比无显著性差异(P>0.05)。此外,DGGE条带测序表明Lacto-bacillus plantarum WCFS1在灌胃期间可在大部分小鼠肠道内占据优势地位。
Both traditional culture-dependent method and PCR-Denaturing Gradient Gel Electrophoresis（PCR-DGGE） were used to evaluate the effects of Lactobacillus plantarum WCFS1 on the amount and species of microflora in mice intestinal tract.It is observed that the amount of Lactobacillus in mice intestine during the feeding and recovery stages significantly increased（P〈0.05） when compared with the control stage;while the amount of Enterococcus decreased（P〈0.01）.The DGGE pattern and diversity analysis indicated that the diversity of microflora at the feeding stage had great increase（P〈0.01）,however,this situation changed and the diversity of microflora showed no difference between control stage and treated stage within 7 days.Furthermore,the sequencing of DGGE band showed that Lactobacillus plantarum WCFS1 occupied the dominant position in the intestinal microflora of the major testing mice during the feeding stage.
出处
《南昌大学学报（理科版）》
CAS
北大核心
2011年第6期559-563,共5页
Journal of Nanchang University(Natural Science)
基金
国家自然科学基金资助项目(30860008
31000048
30900038)
江西省自然科学基金资助项目(0630143)
江西省主要学科学术带头人培养项目(2009)
