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Controllingagricultural product price volatility:An empirical analysis fromCameroon
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作者 Ivette Gnitedem KEUBENG George Achu MULUH Vatis Christian KEMEZANG 《Regional Sustainability》 2025年第2期102-116,共15页
Motivated by a significant impact of price volatility on food security and economic stability inCameroon,this study aims to understand the factors influencing agricultural product price volatility(APPV)and formulateef... Motivated by a significant impact of price volatility on food security and economic stability inCameroon,this study aims to understand the factors influencing agricultural product price volatility(APPV)and formulateeffective policies for mitigating its negative impactand promoting sustainable economic growth.Specifically,this research used theautoregressive distributed lag-error correction model(ARDL-ECM)to analyse the impact of agricultural productivity,agricultural product imports,population,temperature variation,gross domestic product(GDP)per capita,and government expenditure on APPV based on the annual data from 2000 to 2021.The ARDL-ECM estimation results revealed that agricultural productivity(β=4.901),agricultural product imports(β=1.012),population(β=13.635),and GDP per capita(β=2.794)were positively related toAPPV,while temperature variation(β=-0.990)and government expenditure(β=-8.585)were negatively related toAPPVin the long term.However,temperature variation had a positive relationship with APPV in the short term.Moreover,the Granger causality test showed that there werebidirectional causality of APPV with agricultural productivityandagricultural product imports,and unidirectional causality of APPVwith population,temperature variation,GDP per capita,and government expenditure.The findings highlight the importance of public policies in stabilizing agricultural product prices by investing in agricultural research,improving access to agricultural inputs,strengthening farmer capacities,implementing climate adaptation measures,and enhancing rural infrastructure.Thesepolicies can reduce APPV,improve food security,and promote inclusive economic growth in Cameroon. 展开更多
关键词 Agricultural product price volatility(appv) Autoregressive distributed lag-error correction model(ARDL-ECM) Food security Agricultural productivity Climate change
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Coin impact on cross‑crypto realized volatility and dynamic cryptocurrency volatility connectedness
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作者 Burak Korkusuz Mehmet Sahiner 《Financial Innovation》 2025年第1期3732-3763,共32页
This study evaluates the predictive accuracy of traditional time series(TS)models versus machine learning(ML)methods in forecasting realized volatility across major cryptocurrencies—Bitcoin(BTC),Ethereum(ETH),Litecoi... This study evaluates the predictive accuracy of traditional time series(TS)models versus machine learning(ML)methods in forecasting realized volatility across major cryptocurrencies—Bitcoin(BTC),Ethereum(ETH),Litecoin(LTC),and Ripple(XRP).Employing high-frequency data,we analyze cross-cryptocurrency volatility dynamics through two complementary approaches:volatility forecasting and connectedness analysis.Our findings reveal three key insights:(i)TS models,particularly the heterogeneous autoregressive(HAR)model,exhibit superior predictive performance over their ML counterparts,with the long short-term memory(LSTM)model providing competitive yet inconsistent results due to overfitting and short-term volatility challenges;(ii)including lagged realized volatility of large-cap coins improves predictive accuracy for mid-cap coins,especially XRP,whereas forecasts for largecap coins remain stable,indicating more resilient volatility patterns;and(iii)volatility connectedness analysis reveals substantial spillover effects,particularly pronounced during market turmoil,with large-cap assets(BTC and ETH)acting as primary volatility transmitters and mid-cap assets(XRP and LTC)serving as volatility receivers.These results contribute to the understanding of volatility forecasting and risk management in cryptocurrency markets,offering implications for investors and policymakers in managing market risk and interdependencies in digital asset portfolios. 展开更多
关键词 volatility forecasting Realized volatility Bitcoin Cross-cryptocurrency impact Dynamic connectedness Machine learning Network analysis Econometric models
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Pricing Multi-Strike Quanto Call Options on Multiple Assets with Stochastic Volatility, Correlation, and Exchange Rates
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作者 Boris Ter-Avanesov Gunter Meissner 《Applied Mathematics》 2025年第1期113-142,共30页
Quanto options allow the buyer to exchange the foreign currency payoff into the domestic currency at a fixed exchange rate. We investigate quanto options with multiple underlying assets valued in different foreign cur... Quanto options allow the buyer to exchange the foreign currency payoff into the domestic currency at a fixed exchange rate. We investigate quanto options with multiple underlying assets valued in different foreign currencies each with a different strike price in the payoff function. We carry out a comparative performance analysis of different stochastic volatility (SV), stochastic correlation (SC), and stochastic exchange rate (SER) models to determine the best combination of these models for Monte Carlo (MC) simulation pricing. In addition, we test the performance of all model variants with constant correlation as a benchmark. We find that a combination of GARCH-Jump SV, Weibull SC, and Ornstein Uhlenbeck (OU) SER performs best. In addition, we analyze different discretization schemes and their results. In our simulations, the Milstein scheme yields the best balance between execution times and lower standard deviations of price estimates. Furthermore, we find that incorporating mean reversion into stochastic correlation and stochastic FX rate modeling is beneficial for MC simulation pricing. We improve the accuracy of our simulations by implementing antithetic variates variance reduction. Finally, we derive the correlation risk parameters Cora and Gora in our framework so that correlation hedging of quanto options can be performed. 展开更多
关键词 Quanto Option Multi-Strike Option Stochastic volatility (SV) Stochastic Correlation (SC) Stochastic Exchange Rates (SER) CORA GORA Correlation Risk
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Bitcoin’s Weekend Effect: Returns, Volatility, and Volume (2014-2024)
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作者 Zhe Xu 《Proceedings of Business and Economic Studies》 2025年第5期54-61,共8页
Using daily BTC-USD data from September 19,2014 to January 21,2024,this paper re-examines whether weekends differ from weekdays for Bitcoin along three margins:average returns,close-to-close volatility,and trading act... Using daily BTC-USD data from September 19,2014 to January 21,2024,this paper re-examines whether weekends differ from weekdays for Bitcoin along three margins:average returns,close-to-close volatility,and trading activity.We implement Welch mean comparisons and HAC-robust OLS with month fixed effects(bandwidths 5,7,and 14).In the full sample and across subsamples(2016–2019;2020–2023;early 2024),we find no detectable weekend–weekday gap in average returns,while volatility and trading activity are lower on weekends.The patterns are robust to using squared returns as a volatility proxy.The joint evidence is consistent with liquidity and attention mechanisms—quieter weekends rather than compensating return premia.Replication files reproduce all tables and figures. 展开更多
关键词 Bitcoin Weekend effect Day-of-the-week volatility Trading volume HAC Cryptocurrency
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Cryptocurrency Volatility and Its Impact on Emerging Markets: Quantitative Analysis
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作者 Xinyang Kray Wang 《Economics World》 2025年第2期106-112,共7页
Cryptocurrency,a booming decentralised asset designed based on the blockchain architecture,is particularly important to the market at the present time by studying the volatility risk of cryptocurrencies.In this paper,... Cryptocurrency,a booming decentralised asset designed based on the blockchain architecture,is particularly important to the market at the present time by studying the volatility risk of cryptocurrencies.In this paper,we empirically analyse the volatility risk of cryptocurrencies through quantitative analysis models,comprehensively using the Markov state transition GARCH model with skewed distribution(Skew-MSGARCH)and the autoregressive conditional volatility density ARJI model introducing the Poisson jump factor,and selecting the earliest developed and the most mature currency price volatility daily return series,to deeply explore the volatility risk of digital cryptocurrencies.risk.Finally,it can be seen through in-depth analyses that the expectation factor and information inducement are the main reasons leading to the exacerbation of the volatility risk of digital cryptocurrencies.It is recommended that this situation be optimised and improved in terms of the value function of digital cryptocurrencies themselves and the implementation of systematic risk management and regulatory innovation.As an important component of the digital economy,blockchain technology can effectively regulate and improve the volatility of digital cryptocurrencies under macroeconomic policies,thereby maintaining the security and stability of emerging financial markets. 展开更多
关键词 cryptocurrency volatility emerging markets quantitative analysis
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Forecasting cryptocurrency volatility:a novel framework based on the evolving multiscale graph neural network
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作者 Yang Zhou Chi Xie +2 位作者 Gang‑Jin Wang Jue Gong You Zhu 《Financial Innovation》 2025年第1期2484-2535,共52页
Cryptocurrency is a remarkable financial innovation that has affected the financial system in fundamental ways.Its increasingly complex interactions with the conventional financial market make precisely forecasting it... Cryptocurrency is a remarkable financial innovation that has affected the financial system in fundamental ways.Its increasingly complex interactions with the conventional financial market make precisely forecasting its volatility increasingly challenging.To this end,we propose a novel framework based on the evolving multiscale graph neural network(EMGNN).Specifically,we embed a graph that depicts the interactions between the cryptocurrency and conventional financial markets into the predictive process.Furthermore,we employ hierarchical evolving graph structure learners to model the dynamic and scale-specific interactions.We also evaluate our framework’s robustness and discuss its interpretability by extracting the learned graph structure.The empirical results show that(i)cryptocurrency volatility is not isolated from the conventional market,and the embedded graph can provide effective information for prediction;(ii)the EMGNN-based forecasting framework generally yields outstanding and robust performance in terms of multiple volatility estimators,cryptocurrency samples,forecasting horizons,and evaluation criteria;and(iii)the graph structure in the predictive process varies over time and scales and is well captured by our framework.Overall,our work provides new insights into risk management for market participants and into policy formulation for authorities. 展开更多
关键词 Cryptocurrency volatility forecasting Graph neural network Deep learning Multiscale
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Discounted‑likelihood valuation of variance and volatility swaps
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作者 Napat Rujeerapaiboon Sanae Rujivan Hongdan Chen 《Financial Innovation》 2025年第1期536-569,共34页
The valuation of financial derivatives often assumes risk neutrality with respect to the risk-neutral martingale measure,which prevents arbitrage opportunities.However,casual traders may still incur substantial losses... The valuation of financial derivatives often assumes risk neutrality with respect to the risk-neutral martingale measure,which prevents arbitrage opportunities.However,casual traders may still incur substantial losses when trading at this risk-neutral price,especially when the price has to be paid now and the payoff is only realized in the future.This study proposes a new valuation framework that provides risksensitive investors with an additional safeguard.The proposed framework embraces a worst-case perspective while exploiting the underlier’s stochastic process,representing a combination of robust optimization and stochastic programming.Notably,it aims to mitigate losses in the likelier scenarios of the underlying asset’s prices.When the underlier’s returns are independent and lognormally but not necessarily identically distributed,our approach for pricing variance and volatility swaps could be greatly simplified,benefit from parallel computing,and be solved by a two-dimensional grid search.We further derive a closed-form solution in some special stationary cases and provide experimental results to highlight the effect of risk aversion on fending off sizable trading losses. 展开更多
关键词 Variance swaps volatility swaps Derivative pricing Robust optimization Risk aversion
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Power Options Pricing under Markov Regime-Switching Two-Factor Stochastic Volatility Jump-Diffusion Model
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作者 HAN Shu-shu WEI Yu-ming 《Chinese Quarterly Journal of Mathematics》 2025年第1期59-73,共15页
In this paper,we incorporate Markov regime-switching into a two-factor stochastic volatility jump-diffusion model to enhance the pricing of power options.Furthermore,we assume that the interest rates and the jump inte... In this paper,we incorporate Markov regime-switching into a two-factor stochastic volatility jump-diffusion model to enhance the pricing of power options.Furthermore,we assume that the interest rates and the jump intensities of the assets are stochastic.Under the proposed framework,first,we derive the analytical pricing formula for power options by using Fourier transform technique,Esscher transform and characteristic function.Then we provide the efficient approximation to calculate the analytical pricing formula of power options by using the FFT approach and examine the accuracy of the approximation by Monte Carlo simulation.Finally,we provide some sensitivity analysis of the model parameters to power options.Numerical examples show this model is suitable for empirical work in practice. 展开更多
关键词 Power options Markov regime-switching Stochastic volatility Stochastic interest rate Stochastic intensity
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Price volatility spreaders in China's coal market in the carbon neutrality context:an evolution analysis based on a transfer entropy network and rank aggregation
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作者 Chan Liu Han Hu +4 位作者 Zhigang Wang Feng An Xueyong Liu Ze Wang Zhanglu Tan 《International Journal of Coal Science & Technology》 2025年第2期145-157,共13页
This paper investigates China's coal price volatility spreaders(CPVSs)from the supply side to locate the volatility source since coal price volatility may destabilize many downstream products'prices or even br... This paper investigates China's coal price volatility spreaders(CPVSs)from the supply side to locate the volatility source since coal price volatility may destabilize many downstream products'prices or even bring uncertainties to macroeconomic output.Especially in the carbon neutrality context,China's coal market is being reconstructed and responding to imbalances between supply and demand;identifying the CPVSs helps alleviate rising market instability and prevent energy-induced system risk.To achieve this objective,we explore causalities among 938 weekly coal prices reported by different coal-producing areas of China from 2006.9.4 to 2021.7.12 using the transfer entropy method.Then,coal price volatility influence is quantified to identify the CPVSs by conjointly using complex network theory and a rank aggregation method.The validity test demonstrates that the proposed hybrid method efficiently identifies the CPVSs as it correlates to many price determinants,e.g.,electricity and coal consumption and generation.The empirical results show that causalities among coal prices changed dramatically in 2016,2018,and 2020,affected by coal decapacity and carbon neutrality policies.Before 2018,coal-producing provinces with strong demand for coal and electricity,e.g.,Jiangxi,Chongqing,and Sichuan,were CPVSs;after 2019,those with comparative advantages in coal supply,e.g.,Gansu and Ningxia,were CPVSs.Overall,the coal market is unstable and sensitive to energy policy and external shocks.Policymakers and market participants are recommended to monitor and manage the CPVSs to improve energy security,avoid policy-induced instability and prevent risks caused by coal price fluctuations. 展开更多
关键词 Coal price volatility Carbon neutrality Complex network Transfer entropy Aggregate ranking
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Baidu News and the return volatility of Chinese commodity futures:evidence for the sequential information arrival hypothesis
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作者 Ruwei Zhao Xiong Xiong +2 位作者 Junjun Ma Yuzhao Zhang Yongjie Zhang 《Financial Innovation》 2025年第1期2279-2302,共24页
This study uses Baidu News data and introduces a novel proxy for the rate of information flow to examine its relationship with return volatility in Chinese commodity futures and to test two competing hypotheses.We exa... This study uses Baidu News data and introduces a novel proxy for the rate of information flow to examine its relationship with return volatility in Chinese commodity futures and to test two competing hypotheses.We examine the contemporaneous relationships using correlation coefficient analysis,and find apparent differences between the information flow-return volatility relationship and the information flowtrading volume relationship.The empirical evidence contradicts the mixture of distribution hypothesis(MDH)and suggests that the rate of information flow distinctly affects trading volume and volatility.We conducted linear and nonlinear Granger causality tests to explore the sequential information arrival hypothesis(SIAH).The empirical results prove that a lead-lag linear and nonlinear causality exists between the information flow and return volatility of commodity futures,which is consistent with SIAH.In other words,a partial equilibrium exists before reaching the ultimate equilibrium when the new information arrives in the market.Finally,these findings are robust to alternative measurement of return volatility and subperiod analysis.Our findings reject the MDH and support the SIAH in the context of Chinese commodity futures. 展开更多
关键词 Baidu News Chinese commodity futures Return volatility Sequential information arrival hypothesis Mixture of distribution hypothesis
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HS-SPME-GC-MS结合多元统计分析对金银花线香燃烧产物的鉴定
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作者 张颖 及华 +2 位作者 李梦雪 于文龙 章丽 《中国农业科技导报(中英文)》 北大核心 2026年第1期242-254,共13页
为鉴定线香中主要挥发性成分,进一步揭示这些成分在不同样品间的差异及其对香气特征的贡献,采用顶空固相微萃取-气相色谱-质谱联用技术(headspace solid-phase microextraction gas chromatography-mass spectrometry,HS-SPME-GC-MS)结... 为鉴定线香中主要挥发性成分,进一步揭示这些成分在不同样品间的差异及其对香气特征的贡献,采用顶空固相微萃取-气相色谱-质谱联用技术(headspace solid-phase microextraction gas chromatography-mass spectrometry,HS-SPME-GC-MS)结合多元统计分析方法对粘粉燃烧产物、金银花粉、金银花粉燃烧产物、线香燃烧产物中挥发性成分进行提取鉴定,并确定关键挥发性成分。结果表明,从4种样品中共鉴定出102种挥发性成分,以芳香族、杂环类、酮类化合物等成分为主。主成分分析、正交偏最小二乘法判别分析及聚类热图分析表明,金银花粉燃烧产物和线香燃烧产物挥发性成分组成相似,与粘粉燃烧产物及金银花粉挥发性成分存在较大差异,并筛选出25种投影变量的重要性(variable important for the projection,VIP)>1的关键挥发性成分。研究结果为金银花线香的进一步开发提供理论依据,也为中药材的应用拓展了新方向。 展开更多
关键词 金银花 线香 挥发性成分 多元统计分析
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桂枝挥发油对肺炎克雷伯菌的抑菌作用研究
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作者 徐军 陈人萍 +3 位作者 刘卫 高佳 李会影 张英华 《特产研究》 2026年第1期97-101,111,共6页
本文旨在研究桂枝挥发油对肺炎克雷伯菌的体外、体内抑制作用。采用水蒸气蒸馏法提取桂枝挥发油(VORC),琼脂稀释法测定VORC对肺炎克雷伯菌的最低抑菌浓度(MIC);通过生长曲线法检测VORC对肺炎克雷伯菌生长能力的影响;建立肺炎克雷伯菌诱... 本文旨在研究桂枝挥发油对肺炎克雷伯菌的体外、体内抑制作用。采用水蒸气蒸馏法提取桂枝挥发油(VORC),琼脂稀释法测定VORC对肺炎克雷伯菌的最低抑菌浓度(MIC);通过生长曲线法检测VORC对肺炎克雷伯菌生长能力的影响;建立肺炎克雷伯菌诱导大鼠重症肺炎模型,探讨VORC在体内的抗菌作用。结果表明,VORC对肺炎克雷伯菌的MIC为2.5 mg/m L,在体外能显著抑制肺炎克雷伯菌的生长,且抑菌活性呈浓度依赖性;临床表现证实了建模成功;灌胃给予高(0.12mL/kg)、中(0.06mL/kg)剂量VORC后,能显著降低大鼠LI值(P<0.01),明显改善动脉血气指标(P<0.01)且能显著降低肺内活菌数(P<0.05)。本研究表明VORC对肺炎克雷伯菌有较好的抑菌效果。 展开更多
关键词 桂枝 挥发油 抑菌作用 肺炎克雷伯菌
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超声辅助萃取-气质分析甜橙香精挥发性成分方法优化
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作者 张敦铁 邵佩 +2 位作者 庄虎 孙炜炜 李星 《化学研究与应用》 北大核心 2026年第1期212-219,共8页
采用超声波辅助萃取(Ultrasonic extraction,UE)结合气相色谱-质谱联用(GC-MS)技术建立了甜橙香精挥发性成分的分析方法,通过保留指数法(RI)进行定性,各挥发性成分的含量通过内标法进行定量,最佳的萃取条件为:萃取溶剂二氯甲烷,样品量10... 采用超声波辅助萃取(Ultrasonic extraction,UE)结合气相色谱-质谱联用(GC-MS)技术建立了甜橙香精挥发性成分的分析方法,通过保留指数法(RI)进行定性,各挥发性成分的含量通过内标法进行定量,最佳的萃取条件为:萃取溶剂二氯甲烷,样品量100uL,萃取温度45℃,萃取时间50 min,萃取的挥发性成分种类最丰富;采用该方法在甜橙香精中鉴定出挥发性成分33种,其中烯烃类化合物是甜橙香精中主要挥发性成分;通过相对气味活度值(ROAV值)分析得到甜橙香精中的关键特征风味物质是月桂烯、香茅醛、柠檬醛、月桂醛、芳樟醇、薄荷脑、L-薄荷酮、香芹酮、乙酸-3-苯基丙酯。 展开更多
关键词 甜橙香精 超声辅助萃取 挥发性成分 相对气味活度值
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植物活墙净化VOCs效能:机器学习预测与优化
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作者 陈秋瑜 熊强伟 刘小虎 《南方建筑》 北大核心 2026年第1期12-21,共10页
室内挥发性有机化合物(VOCs)严重危害人体健康。为精准预测植物活墙对VOCs的净化效能并指导其优化配置,通过多参数实验构建数据集,采用机器学习方法对比六种算法,可解释技术解析参数影响,并运用多目标优化方法生成配置方案。确定LightGB... 室内挥发性有机化合物(VOCs)严重危害人体健康。为精准预测植物活墙对VOCs的净化效能并指导其优化配置,通过多参数实验构建数据集,采用机器学习方法对比六种算法,可解释技术解析参数影响,并运用多目标优化方法生成配置方案。确定LightGBM为最优模型,模型动态预测精度达R^(2)=0.85。参数影响力排序为背景浓度>温度>湿度>活墙面积>植物密度>植物种类,其中温度存在阈值效应,植物净化效率排序为薄荷>吊兰>绿萝。基于此提出了三类优化策略及参数配置表,实现了该技术从经验设计向数据驱动设计的转型,为健康建筑提供了关键技术支撑。 展开更多
关键词 植物活墙 挥发性有机化合物(VOCs) 机器学习 空气质量 可解释性分析 多目标优化
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应收账款对股价波动的影响:“压舱石”还是“引雷针”?
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作者 成春林 肖泽杨 郭进 《华东经济管理》 北大核心 2026年第2期99-109,共11页
文章基于2010-2023年中国上市公司数据,考察企业应收账款对股价波动的影响效应及其内在机理。研究发现:企业应收账款的增加会显著加剧股价波动,产生了“引雷针”效应;作用渠道分析显示,企业应收账款增加通过降低资金运营效率和盈利稳定... 文章基于2010-2023年中国上市公司数据,考察企业应收账款对股价波动的影响效应及其内在机理。研究发现:企业应收账款的增加会显著加剧股价波动,产生了“引雷针”效应;作用渠道分析显示,企业应收账款增加通过降低资金运营效率和盈利稳定性,加剧股价波动;异质性分析表明,企业应收账款对股价波动的影响在劳动密集型、资本密集型、短期应收账款比重较高、公司治理水平较低以及客户重要性较低的企业中更显著。此外,企业信息披露质量和投资者关注度的提升会削弱应收账款的“引雷针”效应,媒体负面新闻报道的增加则放大了其“引雷针”效应。研究结论为企业优化应收账款管理和防范财务风险提供了新的实证支撑和实践启示。 展开更多
关键词 应收账款 股价波动 资金运营效率 盈利稳定性 财务风险
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千斤拔挥发油抗氧化有效部位的筛选及其物质基础的解析
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作者 杨长花 刘虹千 +4 位作者 王月茹 彭修娟 韩萍 李华 刘峰 《西北药学杂志》 2026年第1期42-58,共17页
目的筛选千斤拔挥发油的抗氧化有效部位,并对其化学成分进行气相色谱-质谱联用(gas chromatography-mass spectrometry,GC-MS)解析。方法采用7种溶剂提取法提取千斤拔挥发油,通过体外抗氧化实验评价不同提取工艺所得挥发油的抗氧化活性... 目的筛选千斤拔挥发油的抗氧化有效部位,并对其化学成分进行气相色谱-质谱联用(gas chromatography-mass spectrometry,GC-MS)解析。方法采用7种溶剂提取法提取千斤拔挥发油,通过体外抗氧化实验评价不同提取工艺所得挥发油的抗氧化活性,运用GC-MS技术对各挥发油组分进行化学成分定性分析。结果不同溶剂提取的千斤拔挥发油抗氧化活性存在显著差异,活性由强至弱依次为无水乙醇提取物>甲醇提取物>正丁醇提取物>乙酸乙酯提取物,二氯甲烷、石油醚、正己烷提取物的抗氧化活性较弱。GC-MS分析从7种溶剂提取的挥发油中分别定性出43、48、45、44、40、43、46个化合物。讨论千斤拔挥发油的化学成分与其抗氧化活性的构效关系仍需进一步深入研究。 展开更多
关键词 千斤拔 挥发油 抗氧化 气相色谱串联质谱联用仪 溶剂提取法
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金思维提取物对APPV717I转基因小鼠早期学习记忆和突触结构与功能的影响 被引量:7
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作者 张雷明 田金洲 +7 位作者 尹军祥 时晶 王蓬文 王蓉 胡泉 赵志炜 姬志娟 任映 《中国中药杂志》 CAS CSCD 北大核心 2009年第4期428-432,共5页
目的:研究金思维提取物(GEPT)对APPV717I转基因小鼠痴呆早期学习记忆的影响,并进一步探讨其可能的机制。方法:将3月龄的APPV717I转基因小鼠随机分为模型组、多奈哌齐治疗组(0.92mg·kg-1·d-1)、GEPT低、中、高(0.075,0.15,0.3g... 目的:研究金思维提取物(GEPT)对APPV717I转基因小鼠痴呆早期学习记忆的影响,并进一步探讨其可能的机制。方法:将3月龄的APPV717I转基因小鼠随机分为模型组、多奈哌齐治疗组(0.92mg·kg-1·d-1)、GEPT低、中、高(0.075,0.15,0.3g·kg-1·d-1)剂量组,并以同月龄遗传背景相同的C57BL/6J小鼠作为正常组,每组6只,每天灌胃给药1次。给药4个月后(7月龄)用Morris水迷宫进行行为学测试,用免疫组化方法测定海马CA1区突触相关蛋白Shank1的表达变化,同时用透射电镜观察海马CA1区突触的超微结构变化。结果:行为学检测,GEPT治疗组与模型组相比定位航行实验和空间探索实验均有显著差异(P<0.05)。突触相关蛋白Shank1,模型组小鼠大脑海马CA1区中Shank1阳性细胞总面积以及阳性细胞积分吸光度与正常组相比明显减少,而GEPT治疗组与模型组相比能显著提高Shank1阳性细胞总面积以及阳性细胞积分吸光度(P<0.05)。电镜结果显示,模型组小鼠可见突触数量减少,突触间隙增宽,突触界面曲率下降,突触后致密区厚度减小,GEPT治疗组能剂量依赖性的对突触损害起到修复作用。结论:GEPT能通过修复突触损伤以及提高Shank1蛋白的表达进而改善APPV717I转基因小鼠痴呆早期的学习记忆能力。 展开更多
关键词 金思维提取物 appv717I转基因小鼠 MORRIS水迷宫 Shank1 突触
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中药复方金思维对APPV717Ⅰ转基因小鼠海马神经损伤的保护作用 被引量:4
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作者 张雷明 田金洲 +5 位作者 尹军祥 时晶 王蓬文 王蓉 胡泉 任映 《中草药》 CAS CSCD 北大核心 2009年第3期410-415,共6页
目的研究中药复方金思维对APPV717Ⅰ转基因小鼠海马神经损伤的保护作用。方法将3月龄的APPV717Ⅰ转基因小鼠随机分为模型组,多奈哌齐治疗组(0.92 mg/kg),金思维小、中、大剂量(0.075、0.15、0.3g/kg)治疗组,并以同月龄遗传背景相同的C57... 目的研究中药复方金思维对APPV717Ⅰ转基因小鼠海马神经损伤的保护作用。方法将3月龄的APPV717Ⅰ转基因小鼠随机分为模型组,多奈哌齐治疗组(0.92 mg/kg),金思维小、中、大剂量(0.075、0.15、0.3g/kg)治疗组,并以同月龄遗传背景相同的C57BL/6J小鼠作为正常对照组,每组6只,每天ig给药1次。给药8个月后(11月龄)用Morris水迷宫进行行为学测试,用电镜观察海马CA1区超微结构变化,同时用免疫组化方法观察海马CA1区Shank1蛋白的表达变化。结果行为学检测显示,金思维治疗组与模型组相比逃避潜伏期显著缩短(P<0.05),目标象限游泳时间明显延长(P<0.05、0.01),并且与对照组比较无显著差异。海马超微结构显示,模型组小鼠海马CA1神经元出现明显变性及坏死,突触结构不完整,数量明显减少。而金思维各剂量组与模型组相比,剂量依赖性的减轻神经元变性、坏死,增加突触的数目。突触相关蛋白Shank1,模型组小鼠海马CA1区Shank1阳性细胞总数、总面积以及阳性细胞积分吸光度与对照组相比明显减少(P<0.05、0.01),而金思维治疗组与模型组相比能显著提高Shank1阳性细胞总数、总面积以及阳性细胞积分吸光度(P<0.05、0.01)。结论金思维能明显改善APPV717Ⅰ转基因小鼠海马神经损伤,增加突触相关蛋白Shank1的表达,进而改善了APPV717Ⅰ转基因小鼠的学习记忆能力。 展开更多
关键词 复方金思维 appv717 Ⅰ转基因小鼠 突触 Shank1
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补肾化痰益智方对APPV717I小鼠学习记忆能力和海马神经元Tau蛋白磷酸化的影响 被引量:10
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作者 孔明望 赵健邦 +2 位作者 石和元 刘萍 王平 《中国实验方剂学杂志》 CAS CSCD 北大核心 2017年第12期96-102,共7页
目的:观察补肾化痰益智方(BSHT)对APPV717I转基因小鼠干预效果,揭示其可能作用机制。方法:将3月龄APPV717I转基因小鼠120只分为2批,每批60只,每批又随机分为5组,分别为多奈哌齐(0.000 92 g·kg^(-1)·d^(-1))组,补肾化痰益智方... 目的:观察补肾化痰益智方(BSHT)对APPV717I转基因小鼠干预效果,揭示其可能作用机制。方法:将3月龄APPV717I转基因小鼠120只分为2批,每批60只,每批又随机分为5组,分别为多奈哌齐(0.000 92 g·kg^(-1)·d^(-1))组,补肾化痰益智方低、中、高剂量组(5.2,10.4,20.8 g·kg^(-1)·d^(-1)),模型组(APP),每组12只(雌雄各半);同月龄遗传背景相同的C57BL/6J小鼠24只作为正常组,灌胃给药,模型组及正常组予等容积双蒸水灌胃,每日1次。第1批小鼠给药8个月,第2批给药4个月,最后同时以Morris水迷宫进行行为学检测,分别以酶联免疫吸附测定(ELISA)和免疫印迹法测定(Western blot)海马神经元Tau蛋白磷酸化(p-Ser 199/202,Tau-1)的表达水平。结果:行为学实验结果表明,与正常组比较,模型组小鼠的潜伏期有所延长(P<0.05,P<0.01);各治疗组与模型组比潜伏期明显缩短(P<0.05)。ELISA和Western blot实验结果表明,与正常组比较,模型组小鼠海马神经元的Ser199/202磷酸化位点磷酸化程度显著升高(P<0.01);各治疗组Ser199/202磷酸化位点磷酸化水平较模型组比明显缩短(P<0.05,P<0.01)。结论:补肾化痰益智方可以改善Ser199/202磷酸化位点磷酸化程度,防止神经原纤维缠结(NFT)的形成,从而改善APPV717I转基因小鼠早期的学习记忆能力。 展开更多
关键词 补肾化痰益智方 老年性痴呆 appv717I转基因小鼠 MORRIS水迷宫 学习记忆能力 神经原纤维缠结 TAU蛋白磷酸化
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中药金思维对APPV717I转基因小鼠早期学习记忆能力和CaMK Ⅱ表达水平的影响 被引量:3
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作者 尹龙 田金洲 +4 位作者 时晶 王蓬文 孔明望 曾垂友 任映 《中华中医药杂志》 CAS CSCD 北大核心 2011年第8期1822-1825,共4页
目的:通过观察金思维对APPV717I转基因小鼠早期空间学习记忆能力及突触蛋白表达水平的影响,揭示其可能的作用机制。方法:将3月龄APPV717I转基因小鼠60只随机分为模型组、多奈哌齐组(0.00092g.kg-1.d-1)、金思维小(0.075g.kg-1.d-1)、中(... 目的:通过观察金思维对APPV717I转基因小鼠早期空间学习记忆能力及突触蛋白表达水平的影响,揭示其可能的作用机制。方法:将3月龄APPV717I转基因小鼠60只随机分为模型组、多奈哌齐组(0.00092g.kg-1.d-1)、金思维小(0.075g.kg-1.d-1)、中(0.15g.kg-1.d-1)和大剂量组(0.3g.kg-1.d-1),每组12只;同月龄遗传背景相同的C57BL/6J小鼠12只作为正常组,灌胃给药(0.01mL/g体质量),每日1次。在给药4个月后(7月龄)以Morris水迷宫进行行为学检测,以免疫组化的方法测定海马CA1区突触蛋白CaMKⅡ的表达水平。结果:行为学实验结果表明,与模型组比较,GEPT各剂量组能不同程度改善APPV717I转基因小鼠的空间学习记忆能力(P<0.05),免疫组化结果显示GEPT小剂量组CaMKⅡ平均光密度与模型组比较明显增加(P<0.05)。结论:GEPT可能通过提高突触相关蛋白CaMKⅡ在海马CA1区的表达从而改善APPV717I转基因小鼠早期的学习记忆能力。 展开更多
关键词 金思维 appv717I转基因小鼠 MORRIS水迷宫 钙/钙调蛋白依赖蛋白激酶Ⅱ
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