The dominant genic male sterility （DGMS） gene CDMs399-3 derived from a spontaneous mutation in the line 79-399-3 of spring cabbage （Brassica oleracea var. capitata L.）, has been successfully applied in hybrid seed...The dominant genic male sterility （DGMS） gene CDMs399-3 derived from a spontaneous mutation in the line 79-399-3 of spring cabbage （Brassica oleracea var. capitata L.）, has been successfully applied in hybrid seed production of several cabbage cultivars in China. During the development of dominant male sterility lines in cabbage, the conventional identification of homozygous male-sterile plants （CDMs399-3/CDMs399-3） is a laborious and time-consuming process. For marker-assisted selection （MAS） of the gene CDMs399-3 transferred into key spring cabbage line 397, expressed sequence tag-simple sequence repeats （EST-SSR） and SSR technology were used to identify markers that were linked to CDMs399-3 based on method of bulked segregant analysis （BSA）. By screening a set of 978 EST-SSRs and 395 SSRs, a marker BoE332 linked to the CDMs399-3 at a distance of 3.6 cM in the genetic background of cabbage line 397 were identified. 7 homozygons male-sterile plants in population P1170 with 20 plants were obtained finally via MAS of BoE332. Thus, BoE332 will greatly facilitate the transferring of the gene CDMs399-3 into the key spring cabbage line 397 and improve the application of DGMS in cabbage hybrid breeding.展开更多
以紫甘蓝(Brassica oleracea var. capitata rubra)花青素为原料制备智能标签,探讨其在猪肉新鲜度监测中的应用效果。设置常温(25~30℃)和冷藏(4℃)2种储藏条件,通过测定猪肉的挥发性盐基氮(TVB-N)含量、pH、菌落数等新鲜度指标,并结合...以紫甘蓝(Brassica oleracea var. capitata rubra)花青素为原料制备智能标签,探讨其在猪肉新鲜度监测中的应用效果。设置常温(25~30℃)和冷藏(4℃)2种储藏条件,通过测定猪肉的挥发性盐基氮(TVB-N)含量、pH、菌落数等新鲜度指标,并结合感官评价与色差分析,系统探究花青素智能标签的颜色变化与猪肉新鲜度之间的相关性。结果表明,随着储藏时间的延长,猪肉中TVB-N含量和菌落数均呈上升趋势。TVB-N的积累和微生物的生长代谢会导致猪肉储藏环境pH发生变化。色差分析表明,色差与TVB-N含量的拟合度高(R2>0.900 00)。紫甘蓝花青素智能标签在不同储藏温度下均能有效监测猪肉的新鲜度变化。展开更多
基金supported by the National Science and Technology Ministry of China (2008BADB1B02 and 2009BADB8B03)the Core Research Budget of the Non-profit Governmental Research Institution (ICS, CAAS) (1610032011011)+1 种基金the China Agriculture Research System (CARS-25)the National High Technology Research and Development Program of China (863 Program, 2012AA100101)
文摘The dominant genic male sterility （DGMS） gene CDMs399-3 derived from a spontaneous mutation in the line 79-399-3 of spring cabbage （Brassica oleracea var. capitata L.）, has been successfully applied in hybrid seed production of several cabbage cultivars in China. During the development of dominant male sterility lines in cabbage, the conventional identification of homozygous male-sterile plants （CDMs399-3/CDMs399-3） is a laborious and time-consuming process. For marker-assisted selection （MAS） of the gene CDMs399-3 transferred into key spring cabbage line 397, expressed sequence tag-simple sequence repeats （EST-SSR） and SSR technology were used to identify markers that were linked to CDMs399-3 based on method of bulked segregant analysis （BSA）. By screening a set of 978 EST-SSRs and 395 SSRs, a marker BoE332 linked to the CDMs399-3 at a distance of 3.6 cM in the genetic background of cabbage line 397 were identified. 7 homozygons male-sterile plants in population P1170 with 20 plants were obtained finally via MAS of BoE332. Thus, BoE332 will greatly facilitate the transferring of the gene CDMs399-3 into the key spring cabbage line 397 and improve the application of DGMS in cabbage hybrid breeding.
