Alternative polyadenylation(APA)is a post-transcriptional process that typically determines the length of mature mRNAs'3'untranslated region(3'UTR).The global shortened 3'UTR alters mRNA stability,loca...Alternative polyadenylation(APA)is a post-transcriptional process that typically determines the length of mature mRNAs'3'untranslated region(3'UTR).The global shortened 3'UTR alters mRNA stability,localization,and translation and contributes to cancer progression.1 The APA characteristics and its effects on acute myeloid leukemia(AML)remain to be comprehensively described.Here,we identified churchill domain containing 1(CHURC1)as an upregulated gene with a lengthened 3'UTR in CD34+enriched AML cells compared with healthy hematopoietic stem and progenitor cells.As for APA regulation of CHURC1,we found that rs6745 and RNA binding protein TIA1 potentially contribute to the distal poly(A)site usage in cis-and transways,respectively.Mechanistically,a lengthened CHURC13'UTR affected the apoptosis of AML cells by disrupting the expression of DICER1 by sponging miR-186-5p.The chemotherapeutic sensitivity of 8 drugs was associated with CHURC1 poly(A)site usage.Overall,our analysis indicated a previously undetected gene,CHURC1,that affected AML progression through 3'UTR length change resulting from APA.Data collection and detailed analysis methods were described in the supplementary material.展开更多
Persistent heavy rainfall events （PHREs） over South China during 1981 2014 were selected and classified by an objective method, based on the daily precipitation data at 752 stations in China. The circulation charact...Persistent heavy rainfall events （PHREs） over South China during 1981 2014 were selected and classified by an objective method, based on the daily precipitation data at 752 stations in China. The circulation characteristics, as well as the dry-cold air and moisture sources of each type of PHREs were examined. The main results are as follows. A total of 32 non-typhoon influenced PHREs in South China were identified over the study period. By correlation analysis, the PHREs are divided into three types： SC-A type, with its main rainbelt located in the coastal areas and the northeast of Guangdong Province; SC-B type, with its main rainbelt between Guangdong Province and Guangxi Region; and SC-C type, with its main rainbelt located in the north of Guangxi Region. For the SC-A events, dry-cold air flew to South China under the steering effect of troughs in the middle troposphere which originated from the Ural Mountains and West Siberia Plain; whereas, the SC-C events were not influenced by the cold air from high latitudes. There were three water vapor pathways from low-latitude areas for both the SC-A and SC-C PHREs. The tropical Indian Ocean was the main water vapor source for these two PHRE types, while the South China Sea also contributed to the SC-C PHREs. In addition, the SC-A events were also influenced by moist and cold air originating from the Yellow Sea. Generally, the SC-C PHREs belonged to a warm-sector rainfall type, whose precipitation areas were dominated by southwesterly wind, and the convergence in wind speed was the main reason for precipitation.展开更多
基金supported by the Technology Innovation Leading Program of Shaanxi,China(No.2023KXJ-219)the Science and Technology Program of Shaanxi Province,China(No.2022LL-ZD-01HZ).
文摘Alternative polyadenylation(APA)is a post-transcriptional process that typically determines the length of mature mRNAs'3'untranslated region(3'UTR).The global shortened 3'UTR alters mRNA stability,localization,and translation and contributes to cancer progression.1 The APA characteristics and its effects on acute myeloid leukemia(AML)remain to be comprehensively described.Here,we identified churchill domain containing 1(CHURC1)as an upregulated gene with a lengthened 3'UTR in CD34+enriched AML cells compared with healthy hematopoietic stem and progenitor cells.As for APA regulation of CHURC1,we found that rs6745 and RNA binding protein TIA1 potentially contribute to the distal poly(A)site usage in cis-and transways,respectively.Mechanistically,a lengthened CHURC13'UTR affected the apoptosis of AML cells by disrupting the expression of DICER1 by sponging miR-186-5p.The chemotherapeutic sensitivity of 8 drugs was associated with CHURC1 poly(A)site usage.Overall,our analysis indicated a previously undetected gene,CHURC1,that affected AML progression through 3'UTR length change resulting from APA.Data collection and detailed analysis methods were described in the supplementary material.
基金Supported by the National(Key)Basic Research and Development(973)Program of China(2012CB417201)National Natural Science Foundation of China(41375053)
文摘Persistent heavy rainfall events （PHREs） over South China during 1981 2014 were selected and classified by an objective method, based on the daily precipitation data at 752 stations in China. The circulation characteristics, as well as the dry-cold air and moisture sources of each type of PHREs were examined. The main results are as follows. A total of 32 non-typhoon influenced PHREs in South China were identified over the study period. By correlation analysis, the PHREs are divided into three types： SC-A type, with its main rainbelt located in the coastal areas and the northeast of Guangdong Province; SC-B type, with its main rainbelt between Guangdong Province and Guangxi Region; and SC-C type, with its main rainbelt located in the north of Guangxi Region. For the SC-A events, dry-cold air flew to South China under the steering effect of troughs in the middle troposphere which originated from the Ural Mountains and West Siberia Plain; whereas, the SC-C events were not influenced by the cold air from high latitudes. There were three water vapor pathways from low-latitude areas for both the SC-A and SC-C PHREs. The tropical Indian Ocean was the main water vapor source for these two PHRE types, while the South China Sea also contributed to the SC-C PHREs. In addition, the SC-A events were also influenced by moist and cold air originating from the Yellow Sea. Generally, the SC-C PHREs belonged to a warm-sector rainfall type, whose precipitation areas were dominated by southwesterly wind, and the convergence in wind speed was the main reason for precipitation.
