W/O/W multi-structure emulsions were prepared by two-step method,to investigate the effect of different W/O emulsifier,anionic emulsifier as well as the first phase volume ratio on the formation and stability of multi...W/O/W multi-structure emulsions were prepared by two-step method,to investigate the effect of different W/O emulsifier,anionic emulsifier as well as the first phase volume ratio on the formation and stability of multi-structure emulsions.The result indicated that ES-5300 is efficient in forming the homogeneous and stable W/O/W multi-structure emulsions which related to the emulsifier structure and low HLB value.Compared with other anionic emulsifier,sodium isostearoyl lactylate can product more stable multi-structure emulsions.However,the higher temperature stability was affected by the increasing of it.In addition,appropriate first phase volume ratio is critical to the stability of multi-structure emulsions.The multi-structure emulsions with better encapsulation efficiency and great performance in higher temperature was achieved when the first phase volume ratio equal to 1.展开更多
This paper presents a fully integrated platform that leverages hardware,software,and specially formulated O/W emulsions to provide localized mechanical stimuli for manipulating cellular behaviors.The system comprises ...This paper presents a fully integrated platform that leverages hardware,software,and specially formulated O/W emulsions to provide localized mechanical stimuli for manipulating cellular behaviors.The system comprises a hexapole magnetic tweezer device,position-based current calculation software,and biocompatible micro-robots embedded with magnetic microbeads for vibration-driven force generation.High-permeability materials in the tweezer tips,combined with fast-response current regulators,enable rapid and precise force control,ensuring uniform and continuous mechanical stimuli in the pico-newton range.Closed-loop control algorithms automatically adjust coil currents based on the micro-robot’s position,thereby compensating for potential hysteresis and optimizing system stability.Experimental results demonstrate stable operation at frequencies up to 4 Hz,with a theoretical possibility of extending to 8 Hz under a 2 A current,delivering mean forces around 20 pN at 1 Hz with a 57μm emulsion.Additionally,the platform allows fine-tuning of forces by altering emulsion size or bead concentrations,thereby providing researchers with a versatile approach to study apoptosis,proliferation,and the other mechanotransduction pathways.The biodegradable and cell-friendly emulsion serves as a protective membrane for the magnetic microbeads while effectively mimicking the mechanical properties of living cells.By bridging the gap between precise motion control and continuous vibrational force application,this novel platform offers a promising tool for advancing targeted cellular studies,fostering insights into tissue engineering,and improving cancer therapies.展开更多
The stabilizing conditions of W/O microemulsion of Tx 6～C 4H 9OH/c C 6H 12 /H 2O system were studied by visual observation and electrical conductance.The result shows that when the weight ratio of Tx 6 and C 4H 9OH i...The stabilizing conditions of W/O microemulsion of Tx 6～C 4H 9OH/c C 6H 12 /H 2O system were studied by visual observation and electrical conductance.The result shows that when the weight ratio of Tx 6 and C 4H 9OH is 4∶1,the W/O microemulsion sytem has stabilizing and extensive regions,and the system is insensitive to the values of pH at room temperature.In accordance with the theoretical value of HLB,the suitable value of HLB for Tx 6 and C 4H 9OH to compose microemulsion is greater than 9,less than or equal to 10.展开更多
文摘W/O/W multi-structure emulsions were prepared by two-step method,to investigate the effect of different W/O emulsifier,anionic emulsifier as well as the first phase volume ratio on the formation and stability of multi-structure emulsions.The result indicated that ES-5300 is efficient in forming the homogeneous and stable W/O/W multi-structure emulsions which related to the emulsifier structure and low HLB value.Compared with other anionic emulsifier,sodium isostearoyl lactylate can product more stable multi-structure emulsions.However,the higher temperature stability was affected by the increasing of it.In addition,appropriate first phase volume ratio is critical to the stability of multi-structure emulsions.The multi-structure emulsions with better encapsulation efficiency and great performance in higher temperature was achieved when the first phase volume ratio equal to 1.
基金part supported by JSPS KAKENHI Grant Number JP23K28396.
文摘This paper presents a fully integrated platform that leverages hardware,software,and specially formulated O/W emulsions to provide localized mechanical stimuli for manipulating cellular behaviors.The system comprises a hexapole magnetic tweezer device,position-based current calculation software,and biocompatible micro-robots embedded with magnetic microbeads for vibration-driven force generation.High-permeability materials in the tweezer tips,combined with fast-response current regulators,enable rapid and precise force control,ensuring uniform and continuous mechanical stimuli in the pico-newton range.Closed-loop control algorithms automatically adjust coil currents based on the micro-robot’s position,thereby compensating for potential hysteresis and optimizing system stability.Experimental results demonstrate stable operation at frequencies up to 4 Hz,with a theoretical possibility of extending to 8 Hz under a 2 A current,delivering mean forces around 20 pN at 1 Hz with a 57μm emulsion.Additionally,the platform allows fine-tuning of forces by altering emulsion size or bead concentrations,thereby providing researchers with a versatile approach to study apoptosis,proliferation,and the other mechanotransduction pathways.The biodegradable and cell-friendly emulsion serves as a protective membrane for the magnetic microbeads while effectively mimicking the mechanical properties of living cells.By bridging the gap between precise motion control and continuous vibrational force application,this novel platform offers a promising tool for advancing targeted cellular studies,fostering insights into tissue engineering,and improving cancer therapies.
文摘The stabilizing conditions of W/O microemulsion of Tx 6～C 4H 9OH/c C 6H 12 /H 2O system were studied by visual observation and electrical conductance.The result shows that when the weight ratio of Tx 6 and C 4H 9OH is 4∶1,the W/O microemulsion sytem has stabilizing and extensive regions,and the system is insensitive to the values of pH at room temperature.In accordance with the theoretical value of HLB,the suitable value of HLB for Tx 6 and C 4H 9OH to compose microemulsion is greater than 9,less than or equal to 10.
