Powdery mildew negatively impacts wheat yield and quality.Emmer wheat(Triticum dicoccum),an ancestral species of common wheat,is a gene donor for wheat improvement.Cultivated emmer accession H1-707 exhibited all-stage...Powdery mildew negatively impacts wheat yield and quality.Emmer wheat(Triticum dicoccum),an ancestral species of common wheat,is a gene donor for wheat improvement.Cultivated emmer accession H1-707 exhibited all-stage resistance to powdery mildew over consecutive years.Genetic analysis of H1-707 at the seedling stage revealed a dominant monogenic inheritance pattern,and the underlying gene was designated Pm71.By employing bulked segregant exome sequencing(BSE-Seq)and using 2000 F2:3 families,Pm71 was fine mapped to a 336-kb interval on chromosome arm 6AS by referencing to the durum cv.Svevo RefSeq 1.0.Collinearity analysis revealed high homology in the candidate interval between Svevo and six Triticum species.Among six high-confidence genes annotated within this interval,TRITD6Av1G005050 encoding a GDSL esterase/lipase was identified as a key candidate for Pm71.展开更多
The NAC(NAM,ATAF1/2,and CUC2)is a defense-associated transcription factor(TF)family that positively regulates defense responses to pathogen infection.TaNAC069 positively regulates resistance in wheat to Puccinia triti...The NAC(NAM,ATAF1/2,and CUC2)is a defense-associated transcription factor(TF)family that positively regulates defense responses to pathogen infection.TaNAC069 positively regulates resistance in wheat to Puccinia triticina(Pt).However,the molecular mechanism of its interaction with a Pt effector is not clear.We found that Pt effector Pt-1234 interacts with TaNAC069 to subvert host immunity during Pt infection.Quantitative real-time PCR analysis showed that expression of Pt-1234 was significantly upregulated during the early stage of Pt infection.Protein-mediated cell death assays in wheat showed that the Pt-1234 protein was unable to induce cell death in wheat near-isogenic lines carrying different leaf rust resistance genes,whereas it suppressed BAX-induced cell death in leaves of Nicotiana benthamiana.Silencing of Pt-1234 by host-induced gene silencing(HIGS)significantly reduced the virulence of Pt in the susceptible wheat variety Thatcher.The C subdomain of TaNAC069 was responsible for its interaction with Pt-1234,and the E subdomain was required for TaNAC069-mediated defense responses to Pt in planta.These findings indicate that Pt utilizes Pt-1234 to interact with wheat transcription factor TaNAC069 through its C subdomain,thereby modulating wheat immunity.展开更多
One of agriculture’s major challenges is the low efficiency of phosphate(Pi)use,which leads to increased costs,harmful environmental impacts,and the depletion of phosphorus(P)resources.The TaPHT1;6 gene,which encodes...One of agriculture’s major challenges is the low efficiency of phosphate(Pi)use,which leads to increased costs,harmful environmental impacts,and the depletion of phosphorus(P)resources.The TaPHT1;6 gene,which encodes a high-affinity Pi transporter(PHT),plays a crucial role in Pi absorption and transport.In this study,the promoter and coding regions of three TaPHT1;6 gene copies on chromosomes 5A,5B,and 5D were individually amplified and sequenced from 167 common wheat(Triticum aestivum L.)cultivars.Sequence analysis revealed 16 allelic variation sites within the promoters of TaPHT1;6-5B among these cultivars,forming three distinct haplotypes:Hap1,Hap2,and Hap3.Field trials were conducted over two years to compare wheat genotypes with these haplotypes,focusing on assessing plant dry weight,grain yield,P content,Pi fertilizer absorption efficiency,and Pi fertilizer utilization efficiency.Results indicated that Hap3 represented the favored Pi-efficient haplotype.Dual-luciferase reporter assay demonstrated that the Hap3 promoter,carrying the identified allelic variation sites,exhibited higher gene-driven capability,leading to increased expression levels of the TaPHT1;6-5B gene.We developed a distributed cleaved amplified polymorphic site marker(dCAPS-571)to distinguish Hap3 from the other two haplotypes based on these allelic variation sites,presenting an opportunity for breeding Pi-efficient wheat cultivars.This study successfully identified polymorphic sites on TaPHT1;6-5B associated with Pi efficiency and developed a functional molecular marker to facilitate future breeding endeavors.展开更多
基金financially supported by National Natural Science Foundation of China(32301800,32301923 and 32072053)Wheat Industrial Technology System of Shandong Province(SDAIT-01-01)Key Research and Development Project of Shandong Province(2022LZG002-4,2023LZGC009-4-4).
文摘Powdery mildew negatively impacts wheat yield and quality.Emmer wheat(Triticum dicoccum),an ancestral species of common wheat,is a gene donor for wheat improvement.Cultivated emmer accession H1-707 exhibited all-stage resistance to powdery mildew over consecutive years.Genetic analysis of H1-707 at the seedling stage revealed a dominant monogenic inheritance pattern,and the underlying gene was designated Pm71.By employing bulked segregant exome sequencing(BSE-Seq)and using 2000 F2:3 families,Pm71 was fine mapped to a 336-kb interval on chromosome arm 6AS by referencing to the durum cv.Svevo RefSeq 1.0.Collinearity analysis revealed high homology in the candidate interval between Svevo and six Triticum species.Among six high-confidence genes annotated within this interval,TRITD6Av1G005050 encoding a GDSL esterase/lipase was identified as a key candidate for Pm71.
基金funded by State Key Laboratory of North China Crop Improvement and Regulation(NCCIR2023ZZ-10)the National Natural Science Foundation of China(32172384 and 31501623)+1 种基金the Natural Science Foundation of Hebei(C2020204028)the Science and Technology Research Project of Higher Education of Hebei(ZC2023178).
文摘The NAC(NAM,ATAF1/2,and CUC2)is a defense-associated transcription factor(TF)family that positively regulates defense responses to pathogen infection.TaNAC069 positively regulates resistance in wheat to Puccinia triticina(Pt).However,the molecular mechanism of its interaction with a Pt effector is not clear.We found that Pt effector Pt-1234 interacts with TaNAC069 to subvert host immunity during Pt infection.Quantitative real-time PCR analysis showed that expression of Pt-1234 was significantly upregulated during the early stage of Pt infection.Protein-mediated cell death assays in wheat showed that the Pt-1234 protein was unable to induce cell death in wheat near-isogenic lines carrying different leaf rust resistance genes,whereas it suppressed BAX-induced cell death in leaves of Nicotiana benthamiana.Silencing of Pt-1234 by host-induced gene silencing(HIGS)significantly reduced the virulence of Pt in the susceptible wheat variety Thatcher.The C subdomain of TaNAC069 was responsible for its interaction with Pt-1234,and the E subdomain was required for TaNAC069-mediated defense responses to Pt in planta.These findings indicate that Pt utilizes Pt-1234 to interact with wheat transcription factor TaNAC069 through its C subdomain,thereby modulating wheat immunity.
基金supported by the Shennong Laboratory Project of Henan Province,China(SN01-2022-01)the China Postdoctoral Science Foundation(2023M731006)the Project of Science and Technology of Henan Province,China(232102111104)。
文摘One of agriculture’s major challenges is the low efficiency of phosphate(Pi)use,which leads to increased costs,harmful environmental impacts,and the depletion of phosphorus(P)resources.The TaPHT1;6 gene,which encodes a high-affinity Pi transporter(PHT),plays a crucial role in Pi absorption and transport.In this study,the promoter and coding regions of three TaPHT1;6 gene copies on chromosomes 5A,5B,and 5D were individually amplified and sequenced from 167 common wheat(Triticum aestivum L.)cultivars.Sequence analysis revealed 16 allelic variation sites within the promoters of TaPHT1;6-5B among these cultivars,forming three distinct haplotypes:Hap1,Hap2,and Hap3.Field trials were conducted over two years to compare wheat genotypes with these haplotypes,focusing on assessing plant dry weight,grain yield,P content,Pi fertilizer absorption efficiency,and Pi fertilizer utilization efficiency.Results indicated that Hap3 represented the favored Pi-efficient haplotype.Dual-luciferase reporter assay demonstrated that the Hap3 promoter,carrying the identified allelic variation sites,exhibited higher gene-driven capability,leading to increased expression levels of the TaPHT1;6-5B gene.We developed a distributed cleaved amplified polymorphic site marker(dCAPS-571)to distinguish Hap3 from the other two haplotypes based on these allelic variation sites,presenting an opportunity for breeding Pi-efficient wheat cultivars.This study successfully identified polymorphic sites on TaPHT1;6-5B associated with Pi efficiency and developed a functional molecular marker to facilitate future breeding endeavors.
