BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs ...BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.展开更多
In this paper, a vector regulating principle of the phase and amplitude control PAC method for three-phase grid-connected inverters is presented.To solve the problem of heavy inrush current and slow dynamic response w...In this paper, a vector regulating principle of the phase and amplitude control PAC method for three-phase grid-connected inverters is presented.To solve the problem of heavy inrush current and slow dynamic response when system starts up, the starting voltage prediction control and the current feed-forward control are proposed and used, which improve the dynamic performance of the system in the PAC.The experimental results carried out on a three-phase grid-connected inverter proved the validity of the proposed method.展开更多
Multilevel inverters are gaining popularity in high power applications. This paper proposes a new ladder type structure of cascaded three-phase multilevel inverter with reduced number of power semiconductor devices wh...Multilevel inverters are gaining popularity in high power applications. This paper proposes a new ladder type structure of cascaded three-phase multilevel inverter with reduced number of power semiconductor devices which is used to drive the induction motor. The ultimate aim of the paper is to produce multiple output levels with minimum number of semiconductor devices. This paper uses only 11 switches along with 3 diodes and 4 asymmetrical sources to produce an output voltage of 21 levels. The modulation technique plays a major role in commutation of the switches. Here we implement the multicarrier level shifting pulse width modulation technique to produce the commutation signals for the inverter. The proposed multilevel inverter is used to drive the three-phase induction motor. The mathematical modeling of three-phase induction motor is done using Simulink. Furthermore the PI and fuzzy logic controllers are also used to produce the reference waveform of the level shifting technique which in turn produces the commutation signals of the proposed multilevel converter. The controllers are used to control the speed of the induction motor. The effectiveness of the proposed system is proved with the help of simulation. The simulation is performed in MATLAB/Simulink. From the simulation results, it shows that the proposed multilevel inverter works properly to generate the multilevel output waveform with minimum number of semiconductor devices. The PI and fuzzy logic controller performances are evaluated using the results which indicate that with the help of controllers the harmonics has been reduced and the speed control of induction motor is achieved under different loading conditions.展开更多
When multiple distributed converters are integrated, the high frequency harmonics will randomly accumulate at the point of common coupling(PCC). This paper proposes a new fast global synchronous discontinuous pulse wi...When multiple distributed converters are integrated, the high frequency harmonics will randomly accumulate at the point of common coupling(PCC). This paper proposes a new fast global synchronous discontinuous pulse width modulation(GSDPWM) method of threephase inverters to effectively attenuate the high frequency current harmonics at PCC. Firstly, the basic principle and the realization method of GSDPWM for three-phase inverters are explained, which can be employed for different modulation types. Then a fast calculation method,which can equally derive the minimized total harmonic distortion(THD) of total current, is proposed to release the calculation burden. Finally, MATLAB simulations and experimental results are presented to verify the performance of GSDPWM.展开更多
Since the poor performance of orthogonal binary Pulse Position Modulation (PPM) compared with binary Pulse Amplitude Modulation (PAM), this paper presents a new modulation scheme named Pulse Width Modulation (PWM) for...Since the poor performance of orthogonal binary Pulse Position Modulation (PPM) compared with binary Pulse Amplitude Modulation (PAM), this paper presents a new modulation scheme named Pulse Width Modulation (PWM) for Impulse Radio Ultra-WideBand (IR-UWB) communication systems. This modulation scheme uses pulses with equal amplitude and different widths to carry different information. The receiver employs differences between similarity coefficients among these pulses to distinguish different information. Both theoretical analysis and simulation results verify that this novel scheme has a Signal to Noise Ratio (SNR) gain of about 1.75 dB compared with or- thogonal binary PPM, and has an SNR loss of about 1.4 dB compared with binary PAM. Although both the theoretical analysis and simulations are based on time-hopping multiple access, this modulation scheme can also be applied to other accessing techniques of UWB communication systems.展开更多
In this paper, a three-phase inverter with renewable source input is integrated into a grid in synchronization for power sharing by load. In previous topologies, the DC source connected inverter is not synchronized to...In this paper, a three-phase inverter with renewable source input is integrated into a grid in synchronization for power sharing by load. In previous topologies, the DC source connected inverter is not synchronized to the grid which causes harmonics and voltage distortions damaging the load and the source. In order to ensure power sharing by the load from the inverter and the grid, the inverter needs to be operated in synchronization to the grid with the same voltage mag-nitude, frequency and phase as that of the grid voltage. In this paper, the com-plete power from the load is shared by the three-phase grid and the three-phase inverter module reducing the consumption from the conventional grid. This is achieved using the PLL for the reference angular frequency generation with feedback from grid voltage and is connected to the sinusoidal PWM generator. The PLL is used to generate unit vector template reference signals for the signal generator operating the six-switch inverter. The inverter and grid are inter-connected through LC filter for the reduction of harmonics. The power sharing, voltage, and current graphs with THD analysis are analyzed with the help of the MATLAB software.展开更多
Instead of avoiding occurrence of chaos in motor drives, chaos is positively utilized in this paper. A new chaotic pulse width modulation （PWM） scheme is proposed and implemented for AC motors, which functions to si...Instead of avoiding occurrence of chaos in motor drives, chaos is positively utilized in this paper. A new chaotic pulse width modulation （PWM） scheme is proposed and implemented for AC motors, which functions to significantly suppress harmonic peaks and hence acoustic noise. The key is to employ the Chua＇s circuit for generating a desired chaotic sequence. By using a practical induction motor, computer simulation and experimental results verify that the chaotic PWM has advantages of harmonic peak suppression and simple hardware implementation over the conventional PWM and random PWM.展开更多
A large amount of switching loss occurs in the inverter. From this point of view, an inverter design should be optimized for which size and cost will be minimum along with increasing efficiency. The main aim of this p...A large amount of switching loss occurs in the inverter. From this point of view, an inverter design should be optimized for which size and cost will be minimum along with increasing efficiency. The main aim of this paper is the analysis and development of single-phase and three-phase inverter to design with MOSFET and IGBT as power elements by sinusoidal pulse width modulation (SPWM) technique using MATLAB Simulink software and compare their difference with the practical inverter. This work proposes different multilevel stages for the cascaded H-Bridge inverter to enhance the output voltages. Then compare their performance, harmonic distortion, and frequency spectrum. The hardware of an inverter circuit has been developed using the SG3524 microcontroller. The main goal of this design is to generate a sine wave with fewer harmonics, while keeping the cost and complexity of the circuit low. The designed inverter has undergone testing with different AC loads and is primarily intended for low-power applications, as lamps, fans, and chargers. This design aims to provide a reliable and efficient inverter solution for these specific applications.展开更多
文摘BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.
基金supported by the Shanghai Education Committee Scientific Research Subsidization (Grant No.05AZ30)the Specialized Research Fund for the Doctoral Program of Higher Education (Grant No. 20060280018)
文摘In this paper, a vector regulating principle of the phase and amplitude control PAC method for three-phase grid-connected inverters is presented.To solve the problem of heavy inrush current and slow dynamic response when system starts up, the starting voltage prediction control and the current feed-forward control are proposed and used, which improve the dynamic performance of the system in the PAC.The experimental results carried out on a three-phase grid-connected inverter proved the validity of the proposed method.
文摘Multilevel inverters are gaining popularity in high power applications. This paper proposes a new ladder type structure of cascaded three-phase multilevel inverter with reduced number of power semiconductor devices which is used to drive the induction motor. The ultimate aim of the paper is to produce multiple output levels with minimum number of semiconductor devices. This paper uses only 11 switches along with 3 diodes and 4 asymmetrical sources to produce an output voltage of 21 levels. The modulation technique plays a major role in commutation of the switches. Here we implement the multicarrier level shifting pulse width modulation technique to produce the commutation signals for the inverter. The proposed multilevel inverter is used to drive the three-phase induction motor. The mathematical modeling of three-phase induction motor is done using Simulink. Furthermore the PI and fuzzy logic controllers are also used to produce the reference waveform of the level shifting technique which in turn produces the commutation signals of the proposed multilevel converter. The controllers are used to control the speed of the induction motor. The effectiveness of the proposed system is proved with the help of simulation. The simulation is performed in MATLAB/Simulink. From the simulation results, it shows that the proposed multilevel inverter works properly to generate the multilevel output waveform with minimum number of semiconductor devices. The PI and fuzzy logic controller performances are evaluated using the results which indicate that with the help of controllers the harmonics has been reduced and the speed control of induction motor is achieved under different loading conditions.
文摘When multiple distributed converters are integrated, the high frequency harmonics will randomly accumulate at the point of common coupling(PCC). This paper proposes a new fast global synchronous discontinuous pulse width modulation(GSDPWM) method of threephase inverters to effectively attenuate the high frequency current harmonics at PCC. Firstly, the basic principle and the realization method of GSDPWM for three-phase inverters are explained, which can be employed for different modulation types. Then a fast calculation method,which can equally derive the minimized total harmonic distortion(THD) of total current, is proposed to release the calculation burden. Finally, MATLAB simulations and experimental results are presented to verify the performance of GSDPWM.
文摘Since the poor performance of orthogonal binary Pulse Position Modulation (PPM) compared with binary Pulse Amplitude Modulation (PAM), this paper presents a new modulation scheme named Pulse Width Modulation (PWM) for Impulse Radio Ultra-WideBand (IR-UWB) communication systems. This modulation scheme uses pulses with equal amplitude and different widths to carry different information. The receiver employs differences between similarity coefficients among these pulses to distinguish different information. Both theoretical analysis and simulation results verify that this novel scheme has a Signal to Noise Ratio (SNR) gain of about 1.75 dB compared with or- thogonal binary PPM, and has an SNR loss of about 1.4 dB compared with binary PAM. Although both the theoretical analysis and simulations are based on time-hopping multiple access, this modulation scheme can also be applied to other accessing techniques of UWB communication systems.
文摘In this paper, a three-phase inverter with renewable source input is integrated into a grid in synchronization for power sharing by load. In previous topologies, the DC source connected inverter is not synchronized to the grid which causes harmonics and voltage distortions damaging the load and the source. In order to ensure power sharing by the load from the inverter and the grid, the inverter needs to be operated in synchronization to the grid with the same voltage mag-nitude, frequency and phase as that of the grid voltage. In this paper, the com-plete power from the load is shared by the three-phase grid and the three-phase inverter module reducing the consumption from the conventional grid. This is achieved using the PLL for the reference angular frequency generation with feedback from grid voltage and is connected to the sinusoidal PWM generator. The PLL is used to generate unit vector template reference signals for the signal generator operating the six-switch inverter. The inverter and grid are inter-connected through LC filter for the reduction of harmonics. The power sharing, voltage, and current graphs with THD analysis are analyzed with the help of the MATLAB software.
基金Project supported by the Shanghai Leading Academic Discipline Project (Grant No.T0103)
文摘Instead of avoiding occurrence of chaos in motor drives, chaos is positively utilized in this paper. A new chaotic pulse width modulation （PWM） scheme is proposed and implemented for AC motors, which functions to significantly suppress harmonic peaks and hence acoustic noise. The key is to employ the Chua＇s circuit for generating a desired chaotic sequence. By using a practical induction motor, computer simulation and experimental results verify that the chaotic PWM has advantages of harmonic peak suppression and simple hardware implementation over the conventional PWM and random PWM.
文摘A large amount of switching loss occurs in the inverter. From this point of view, an inverter design should be optimized for which size and cost will be minimum along with increasing efficiency. The main aim of this paper is the analysis and development of single-phase and three-phase inverter to design with MOSFET and IGBT as power elements by sinusoidal pulse width modulation (SPWM) technique using MATLAB Simulink software and compare their difference with the practical inverter. This work proposes different multilevel stages for the cascaded H-Bridge inverter to enhance the output voltages. Then compare their performance, harmonic distortion, and frequency spectrum. The hardware of an inverter circuit has been developed using the SG3524 microcontroller. The main goal of this design is to generate a sine wave with fewer harmonics, while keeping the cost and complexity of the circuit low. The designed inverter has undergone testing with different AC loads and is primarily intended for low-power applications, as lamps, fans, and chargers. This design aims to provide a reliable and efficient inverter solution for these specific applications.
