[Objective] The research aimed to study the transferability of Camellia sinensis EST-SSRs in Theaceae plant.[Method] Seven pairs of EST-SSRs primers which derived from Camellia sinensis EST sequence were used to ampli...[Objective] The research aimed to study the transferability of Camellia sinensis EST-SSRs in Theaceae plant.[Method] Seven pairs of EST-SSRs primers which derived from Camellia sinensis EST sequence were used to amplify the nineteen materials of Theaceae plant.[Result] Five pairs in the seven pairs of EST-SSRs primers could effectively amplify the nineteen tested varieties,and the transferability rate was 71.43%.The amplification rate of Camellia retiacalate FengShanCha,Camellia japouica CaiXia and Camellia retiacalate JuBan was the highest.The amplification rate of Camellia synaptica Sealy and Adinandra sagonica var.wallichiana（oc）Ming was the lowest.Moreover,four pairs in the five pairs of primers which could effectively amplify showed the rich polymorphism whose difference was obvious in the tested materials.[Conclusion] SSR primers which were developed from Camellia sinensis genome had the higher transferability in the different genus and species of Theaceae plant,could be used in the comparative genome research and analysis mark research of Theaceae plant.展开更多
Gastric ulcer is a global health concern nowadays. Adinandra nitida, known as Shibi tea, is a flavonoidrich plant found in South China. A. nitida possesses many healthy properties, such as antioxidation and reducing b...Gastric ulcer is a global health concern nowadays. Adinandra nitida, known as Shibi tea, is a flavonoidrich plant found in South China. A. nitida possesses many healthy properties, such as antioxidation and reducing blood pressure. However, its effects on gastric ulcer have not been investigated. This study aimed to investigate the effects of the Shibi tea water extract(STE) and its main flavonoid camellianin A(CA) in hydrochloric acid(HCl) and ethanol(Et OH)-induced acute gastric ulcer in mice. Administration of CA and STE for continuous two days after stimulation by HCl/Et OH significantly attenuated the deterioration of gastric mucosal damage by lowering the gross gastric mucosal index, histopathological injury index, the oxidative stress, the expression of the inflammatory cytokines TNF-α and IL-6, and the expression of inflammatory mediators i NOS and COX-2. Western blotting and immunohistochemistry analysis showed that CA and STE regulated the inflammatory signaling pathway protein levels of IκB-α and NF-κB. Taken together, our study verified that CA and STE have antioxidative and anti-inflammatory effects in gastric ulcer mice. We propose that A. nitida should be developed as natural functional food for acute gastric ulcer patients base on the gastroprotective effects of STE and its main flavonoid CA.展开更多
The floral development of a critically endangered plant Euryodendron awelsum from Ternstroemioideae(Theaceae)has been observed under scanning electron microscope(SEM)for the first time.The flower of E.awelsum is bisex...The floral development of a critically endangered plant Euryodendron awelsum from Ternstroemioideae(Theaceae)has been observed under scanning electron microscope(SEM)for the first time.The flower of E.awelsum is bisexual and teleianthous.Five sepal primordia are initiated 2/5 spirally and arranged in one whorl when mature,and so are petals.Sepals and petals are initiated in the same order either clockwise or counterclockwise in the same flower.The androecial part is a ring pfimordium in the beginning.The in'st five stamen primordia are initiated on this ring primordium at antisepalous positions and then other stamen primordia appear on two sides of each of the first five pfimordia.Finally a total of 25 stamen primordia are initiated from the ring primordium and arranged in one whorl but could be grouped into 5 fascicles.Three sequentially initiated carpels compose an ovary which has an axile-central placenta with three locules and a truncate and indehiscent stigma.The floral development of E.awelsum is distinctly different from plants in Theoideae.展开更多
基金Supported by"Provincial and Ministerial Key Discipline,Provincial Key Laboratory of University and School Laboratory Sharing Platform" ItemSouthwest Forestry University Key Fund Item(110909)~~
文摘[Objective] The research aimed to study the transferability of Camellia sinensis EST-SSRs in Theaceae plant.[Method] Seven pairs of EST-SSRs primers which derived from Camellia sinensis EST sequence were used to amplify the nineteen materials of Theaceae plant.[Result] Five pairs in the seven pairs of EST-SSRs primers could effectively amplify the nineteen tested varieties,and the transferability rate was 71.43%.The amplification rate of Camellia retiacalate FengShanCha,Camellia japouica CaiXia and Camellia retiacalate JuBan was the highest.The amplification rate of Camellia synaptica Sealy and Adinandra sagonica var.wallichiana（oc）Ming was the lowest.Moreover,four pairs in the five pairs of primers which could effectively amplify showed the rich polymorphism whose difference was obvious in the tested materials.[Conclusion] SSR primers which were developed from Camellia sinensis genome had the higher transferability in the different genus and species of Theaceae plant,could be used in the comparative genome research and analysis mark research of Theaceae plant.
基金funded by the “14th Five-Year Plan” team-building projects of Guangdong Academy Agricultural Sciences (202126TD)National Natural Science Foundation of China (81903319, 81803236, 31800295)+10 种基金Guangdong Science and Technology program (2018KJYZ002)Guangdong Basic and Applied Basic Research Foundation (2020A1515011266)Qingyuan Science and Technology Program (DZXQY021, 181022114566189, 181022114566189, 2019A039, 2020KJJH042)Shaoguan Science and Technology Program (2018CS11902, 2018sn081)Guangzhou Science and Technology Program (202002030202)Zhaoqing Science and Technology Program (2019N001, 2019N013)Maoming Science and Technology Program (mmkj2020045)Zhanjiang Science and Technology Program (2020A03014)Yingde Science and Technology Board (JHXM2018029)Innovation Fund projects of Guangdong Academy of Agricultural Sciences (202115)Special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science (R2019PY-JX004, R2018YJ-YB3002, R2016YJYB3003, R2018PY-QF005, R2018QD-101)。
文摘Gastric ulcer is a global health concern nowadays. Adinandra nitida, known as Shibi tea, is a flavonoidrich plant found in South China. A. nitida possesses many healthy properties, such as antioxidation and reducing blood pressure. However, its effects on gastric ulcer have not been investigated. This study aimed to investigate the effects of the Shibi tea water extract(STE) and its main flavonoid camellianin A(CA) in hydrochloric acid(HCl) and ethanol(Et OH)-induced acute gastric ulcer in mice. Administration of CA and STE for continuous two days after stimulation by HCl/Et OH significantly attenuated the deterioration of gastric mucosal damage by lowering the gross gastric mucosal index, histopathological injury index, the oxidative stress, the expression of the inflammatory cytokines TNF-α and IL-6, and the expression of inflammatory mediators i NOS and COX-2. Western blotting and immunohistochemistry analysis showed that CA and STE regulated the inflammatory signaling pathway protein levels of IκB-α and NF-κB. Taken together, our study verified that CA and STE have antioxidative and anti-inflammatory effects in gastric ulcer mice. We propose that A. nitida should be developed as natural functional food for acute gastric ulcer patients base on the gastroprotective effects of STE and its main flavonoid CA.
基金This workis funded bythe National Science Foundation of China(Grant No30560017)
文摘The floral development of a critically endangered plant Euryodendron awelsum from Ternstroemioideae(Theaceae)has been observed under scanning electron microscope(SEM)for the first time.The flower of E.awelsum is bisexual and teleianthous.Five sepal primordia are initiated 2/5 spirally and arranged in one whorl when mature,and so are petals.Sepals and petals are initiated in the same order either clockwise or counterclockwise in the same flower.The androecial part is a ring pfimordium in the beginning.The in'st five stamen primordia are initiated on this ring primordium at antisepalous positions and then other stamen primordia appear on two sides of each of the first five pfimordia.Finally a total of 25 stamen primordia are initiated from the ring primordium and arranged in one whorl but could be grouped into 5 fascicles.Three sequentially initiated carpels compose an ovary which has an axile-central placenta with three locules and a truncate and indehiscent stigma.The floral development of E.awelsum is distinctly different from plants in Theoideae.
