The polychaete species of Capitella are widely distributed in the China seas,however little is known about Capitella taxonomy,and specimens collected from China have been identifi ed as Capitella capitata(Fabricius,17...The polychaete species of Capitella are widely distributed in the China seas,however little is known about Capitella taxonomy,and specimens collected from China have been identifi ed as Capitella capitata(Fabricius,1780)for more than 50 years.C.capitata was considered to be Arctic and subarctic in distribution,therefore the records of C.capitata in the China seas probably represent other species.A taxonomic study based on the samples collected from the northeast coastal water of Shandong Province reveals a diff erent species,Capitella teleta Blake et al.,2009,which is recorded in the China seas for the fi rst time.Morphologically,C.teleta can be easily distinguished from C.capitata by the absence of neuropodial capillaries on chaetigers 8 and 9.The identity of C.teleta is further supported by genetic distance and phylogenetic analyses assessed from mitochondrial cytochrome c oxidase subunit I(COI)gene.In addition,the regeneration feature of C.teleta was studied through whole mount immunohistochemistry and chemical staining.After amputation,the wound of C.teleta was healed within 24 h,forming a signifi cant regeneration blastema by 3 days post amputation(dpa).By 5 dpa,muscle tissues regenerated,nerve fi bers also extended.By 7 dpa,neurites and muscle tissues are both signifi cantly regenerated.Notably,there are more than ten segments regenerated until 16 dpa.As a highly opportunistic species,Capitella teleta is distributed in China,Japan,Korea,North America,and the Mediterranean.It is expected to be an excellent model for studying developmental genetics and evolution of regeneration.展开更多
The Fox genes encode a group of transcription factors that contain a forkhead domain, which forms a structure known as a winged helix. These transcription factors play a crucial role in several key biological processe...The Fox genes encode a group of transcription factors that contain a forkhead domain, which forms a structure known as a winged helix. These transcription factors play a crucial role in several key biological processes, including development. High-degree identity in the canonical forkhead domain has been used to divide Fox proteins into 23 families （FoxA to FoxS）. We surveyed the genome of three spiralians, the oyster Crassostrea gigas, the limpet Lottia gigantea, and the annelid Capitella teleta. We identified 25 C. gigas fox genes, 21 L. gigantea fox genes, and 25 C. teleta fox genes. The C. gigas fox and L. giganteafox genes represented 19 of the 23 families, whereas FoxI, QI, R, and S were missing. The majority of the Fox families were observed within the C. teletafox genes, with the exception of FoxR and S. In addition, thefoxAB-like gene,foxY-like gene, andfoxH gene were also present in the three genomes. The conserved FoxC-FoxL 1 cluster, observed in mammals, was also found in C. gigas. The diversity of temporal expression patterns observed across the developmental process implies the C. gigasfox genes exert a wide range of functions. Further functional studies are required to gain insight into the evolution of Fox genes in bilaterians.展开更多
基金Supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB42000000)to L Zthe National Natural Science Foundation of China(No.41976088)to L Z+1 种基金the Key Development Project of Centre for Ocean Mega-Research of Science,Chinese academy of science(No.COMS2019R01)to L Zthe Pilot National Laboratory for Marine Science and Technology(No.YJ2019NO01)to L Z。
文摘The polychaete species of Capitella are widely distributed in the China seas,however little is known about Capitella taxonomy,and specimens collected from China have been identifi ed as Capitella capitata(Fabricius,1780)for more than 50 years.C.capitata was considered to be Arctic and subarctic in distribution,therefore the records of C.capitata in the China seas probably represent other species.A taxonomic study based on the samples collected from the northeast coastal water of Shandong Province reveals a diff erent species,Capitella teleta Blake et al.,2009,which is recorded in the China seas for the fi rst time.Morphologically,C.teleta can be easily distinguished from C.capitata by the absence of neuropodial capillaries on chaetigers 8 and 9.The identity of C.teleta is further supported by genetic distance and phylogenetic analyses assessed from mitochondrial cytochrome c oxidase subunit I(COI)gene.In addition,the regeneration feature of C.teleta was studied through whole mount immunohistochemistry and chemical staining.After amputation,the wound of C.teleta was healed within 24 h,forming a signifi cant regeneration blastema by 3 days post amputation(dpa).By 5 dpa,muscle tissues regenerated,nerve fi bers also extended.By 7 dpa,neurites and muscle tissues are both signifi cantly regenerated.Notably,there are more than ten segments regenerated until 16 dpa.As a highly opportunistic species,Capitella teleta is distributed in China,Japan,Korea,North America,and the Mediterranean.It is expected to be an excellent model for studying developmental genetics and evolution of regeneration.
基金Supported by the National Basic Research Program of China(973 Program)(No.2010CB126401)the National Natural Science Foundation of China(No.31402285)+3 种基金the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A405)the Earmarked Fund for Modern Agro-Industry Technology Research System(No.CARS-48)the Taishan Scholars Climbing Program of Shandong Provincethe Oversea Taishan Scholars Program of Shandong Province
文摘The Fox genes encode a group of transcription factors that contain a forkhead domain, which forms a structure known as a winged helix. These transcription factors play a crucial role in several key biological processes, including development. High-degree identity in the canonical forkhead domain has been used to divide Fox proteins into 23 families （FoxA to FoxS）. We surveyed the genome of three spiralians, the oyster Crassostrea gigas, the limpet Lottia gigantea, and the annelid Capitella teleta. We identified 25 C. gigas fox genes, 21 L. gigantea fox genes, and 25 C. teleta fox genes. The C. gigas fox and L. giganteafox genes represented 19 of the 23 families, whereas FoxI, QI, R, and S were missing. The majority of the Fox families were observed within the C. teletafox genes, with the exception of FoxR and S. In addition, thefoxAB-like gene,foxY-like gene, andfoxH gene were also present in the three genomes. The conserved FoxC-FoxL 1 cluster, observed in mammals, was also found in C. gigas. The diversity of temporal expression patterns observed across the developmental process implies the C. gigasfox genes exert a wide range of functions. Further functional studies are required to gain insight into the evolution of Fox genes in bilaterians.
