摘要
海洋底栖多毛类动物小头虫是进行发育生物学和再生演化研究的典型模式物种。然而,始初小头虫中国群体实验室全周期养殖体系尚未建立。研究通过4组实验(低溶解氧无饲料、高溶解氧无饲料、低溶解氧添加饲料、高溶解氧添加饲料)探究溶解氧与饲料对生长发育的影响。结果表明:(1)无饲料添加条件下,将溶解氧浓度从3 mg/L提升至8 mg/L,可显著提高存活率46.67%;添加饲料条件下,提高溶解氧浓度(3→8 mg/L)对存活率无显著影响。低氧环境下,饲料添加能提高存活率54.44%,而高氧环境下则无显著差异。(2)提高溶解氧浓度(3→8 mg/L)和添加饲料均显著促进体长增长。(3)提升溶解氧浓度(3→8 mg/L)在无饲料条件下显著提高性别分化率46.29%,而在有饲料添加条件下提高30.78%。饲料添加本身对性别分化无独立影响,但会部分抵消低溶解氧的抑制效应。(4)饲料和溶解氧两者对雌雄性别比均无显著影响。人工基质环境下,始初小头虫存活率达99%,性别分化率100%,体长1.86 cm,雌雄比1.72∶1.00。综上所述,从提高始初小头虫存活率和促进生长的角度考虑,建议其养殖体系为:使用人工基质,维持溶解氧浓度在8 mg/L,每周投喂复合饲料。该研究为始初小头虫中国群体的养殖管理提供了重要的科学依据和实践指导,也为其功能研究体系的开发和应用提供了支撑。
The marine benthic polychaete Capitella teleta serves as an important model organism for studying developmental biology and regenerative evolution.However,the full-cycle laboratory culture system for the Chinese population of C.teleta has not yet been established.We investigated the effects of dissolved oxygen and feed on the growth and development in four experimental groups:low dissolved oxygen without feed,high dissolved oxygen without feed,low dissolved oxygen with feed,and high dissolved oxygen with feed.Results indicate that:(1)Under conditions without feed supplementation,increasing the dissolved oxygen concentration from 3 to 8 mg/L significantly improved the survival rate by 46.67%;with feed supplementation,increasing the dissolved oxygen concentration(3→8 mg/L)had no significant effect on the survival rate.In a hypoxic environment,feed supplementation increased the survival rate by 54.44%,whereas in a hyperoxic environment,there was no significant difference.(2)Both the increase of the dissolved oxygen concentration(3→8 mg/L)and feed supplementation significantly promoted body length growth.(3)Increasing the dissolved oxygen concentration(3→8 mg/L)significantly enhanced the sex differentiation rate by 46.29%under conditions without feed supplementation,and by 30.78%with feed supplementation.Feed supplementation itself does not have an independent effect on sex differentiation but partially counteracts the inhibitory effect of low dissolved oxygen.(4)Neither feed nor dissolved oxygen had a significant impact on the male-to-female sex ratio.In an artificial substrate environment,the survival rate of C.teleta reached 99%,with a gender differentiation rate of 100%,a body length of 1.86 cm,and a female-to-male ratio of 1.72∶1.00.Therefore,to improve the survival rate and promoting the growth of C.teleta,we suggested that the culture system should be as follows:using artificial substrate,maintaining dissolved oxygen concentration at 8 mg/L,and feeding compound feed every week.This study offered significant scientific basis and practical guidance for the breeding management of the Chinese population of C.teleta,with supports to the development and application of its functional research system.
作者
翁洁羊
李勇男
周冰清
李杰
张琳琳
WENG Jie-Yang;LI Yong-Nan;ZHOU Bing-Qing;LI Jie;ZHANG Lin-Lin(Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture(CAS),Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;Laboratory for Marine Biology and Biotechnology,Qingdao Marine Science and Technology Center,Qingdao 266237,China;University of Chinese Academy of Sciences,Beijing 100049,China)
出处
《海洋与湖沼》
北大核心
2025年第4期957-967,共11页
Oceanologia Et Limnologia Sinica
基金
国家自然科学基金面上项目,42376092号
中国科学院B类战略先导科技专项,XDB42000000号
国家自然科学基金青年项目,42306099号
泰山学者工程资助
国家自然科学基金面上项目,41976088号
青岛海洋科学与技术试点国家实验室山东省专项经费,2022QNLM030004号。
关键词
始初小头虫
饲料
溶解氧
人工基质
Capitella teleta
feed
dissolved oxygen
artificial substrate
