[目的]评价宏基因组二代测序技术(metagenomic next generation sequencing,mNGS)诊断骨关节结核的价值。[方法]回顾性分析2022年1月—2023年6月青岛市公共卫生临床中心确诊为骨关节感染性疾病72例患者的临床资料,其中结核46例(结核组,6...[目的]评价宏基因组二代测序技术(metagenomic next generation sequencing,mNGS)诊断骨关节结核的价值。[方法]回顾性分析2022年1月—2023年6月青岛市公共卫生临床中心确诊为骨关节感染性疾病72例患者的临床资料,其中结核46例(结核组,63.9%),非结核26例(非结核组,36.1%)。比较两组患者的资料,评价3种结核检测方法及组合检测效能。[结果]结核组的ESR[(62.9±38.4)mm/h vs(85.5±42.6)mm/h,P=0.033]、CRP[(38.8±35.3)mg/L vs(75.4±53.6)mg/L,P=0.001]显著低于非结核组,而结核组在常规培养[例,阳性/阴性,(27/19)vs(0/26),P<0.001]、Xpert[例,阳性/阴性,(30/16)vs(0/26),P<0.001]、mNGS阳性率[例,阳性/阴性,(32/14)vs(0/26),P<0.001]均显著高于非结核组。3种结核检测及组合检测效能的比较:与常规培养(敏感度58.7%、阴性预测值57.8%、Kappa值0.506)比较,mNGS(敏感度69.6%、阴性预测值65.0%和Kappa值0.623)和Xpert(敏感度65.2%、阴性预测值61.9%、Kappa值0.575)诊断骨关节结核的效能均显著增加。m NGS分别与常规培养和Xpert行联合试验,诊断效能优于任何一种检测方法。[结论]mNGS可有效检测骨关节感染性疾病病原菌,在骨关节结核诊断中具有重要的诊断价值。展开更多
Room-temperature(RT)terahertz(THz)detection finds widespread applications in security inspection,communication,biomedical imaging,and scientific research.However,the state-of-the-art detection strategies are still lim...Room-temperature(RT)terahertz(THz)detection finds widespread applications in security inspection,communication,biomedical imaging,and scientific research.However,the state-of-the-art detection strategies are still limited by issues such as low sensitivity,narrow response range,slow response speed,complex fabrication techniques,and difficulties in scaling up to large arrays.Here,we present a high-sensitivity,broadband-response,and high-speed RT THz detection strategy by utilizing a deep subwavelength metal–semiconductor–metal(MSM)structure.The spontaneously formed 2-dimensional electron gas(2DEG)at the CdTe/PbTe interface provides a superior transport channel characterized by high carrier concentration,low scattering,and high mobility.The synergy of the electromagnetic induced well effect formed in the MSM structure,and the efficient and rapid transport capabilities of the 2DEG channel give rise to an impressive performance improvement.The proposed 2DEG photodetector exhibits a broad frequency range from 22 to 519 GHz,an ultralow noise equivalent power of 3.0×10^(−14)W Hz^(−1/2)at 166 GHz,and a short response time of 6.7μs.This work provides an effective route for the development of high-performance RT THz detection strategies,paving the way for enhanced THz technology applications.展开更多
基金supported by the National Natural Science Foundation of China(11933006,61805060,U2141240,and 62175045)National Key Research and Development Project of China(2023YFB2806700)+5 种基金Zhejiang Provincial Natural Science Foundation of China(LGF21F050001)Hangzhou Science and Technology Bureau of Zhejiang Province(TD2020002)Hangzhou Key Research and Development Program(2024SZD1A39)Research Funds of Hangzhou Institute for Advanced Study(B02006C019019 and 2022ZZ01007)Zhejiang Provincial Natural Science Foundation of China(no.LD25F040001)Hangzhou Joint Fund of the Zhejiang Provincial Natural Science Foundation of China(no.LHZQN25F050001).
文摘Room-temperature(RT)terahertz(THz)detection finds widespread applications in security inspection,communication,biomedical imaging,and scientific research.However,the state-of-the-art detection strategies are still limited by issues such as low sensitivity,narrow response range,slow response speed,complex fabrication techniques,and difficulties in scaling up to large arrays.Here,we present a high-sensitivity,broadband-response,and high-speed RT THz detection strategy by utilizing a deep subwavelength metal–semiconductor–metal(MSM)structure.The spontaneously formed 2-dimensional electron gas(2DEG)at the CdTe/PbTe interface provides a superior transport channel characterized by high carrier concentration,low scattering,and high mobility.The synergy of the electromagnetic induced well effect formed in the MSM structure,and the efficient and rapid transport capabilities of the 2DEG channel give rise to an impressive performance improvement.The proposed 2DEG photodetector exhibits a broad frequency range from 22 to 519 GHz,an ultralow noise equivalent power of 3.0×10^(−14)W Hz^(−1/2)at 166 GHz,and a short response time of 6.7μs.This work provides an effective route for the development of high-performance RT THz detection strategies,paving the way for enhanced THz technology applications.
