Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effec...Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effects of these 2 bifidobacterial species against Salmonella infection.The results demonstrated that B.longum subsp.infantis was more effective than B.adolescentis in alleviating the severity of infection in newborn rats exposed to Salmonella enterica serovar Typhimurium strain SL1344.B.longum subsp.infantis attenuated intestinal inflammation and mucosal damage induced by Salmonella infection,as well as protecting intestinal nerves and intestinal barrier function through TLR4/My D88 signalling.B.longum subsp.infantis also displayed the potential to modulate gut metabolites by promoting the biosynthesis of unsaturated fatty acids(arachidonic acid,eicosapentaenoic acid andα-linolenic acid)and purine metabolism(guanine,adenine,inosine and adenosine),thereby regulating metabolic disturbances.Additionally,the benefits of B.longum subsp.infantis were also observed in the liver,spleen and brain,improving nerve reflexes and suppressing hepatosplenomegaly.Overall,these findings provide novel insights into the prevention and treatment of gutrelated diseases in newborns,highlighting the potentially significant role of B.longum subsp.infantis in clinical applications.展开更多
Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to ...Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to explore the effects of B.longum subsp.infantis supplementation on interferon-beta(IFN-β)secretion and intestinal barrier improvement in growing mice.Female and male mice were orally administered either saline or B.longum subsp.infantis CCFM1269 or I5TI(1×10^(9) CFU/mice per day,n=8)from 1-week-age until 3-,4-,and 5-week-age.RNA sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2'-5'oligoadenylate synthetase(OAS).Additionally,CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling pathway.However,this effect was not observed in 4-and 5-week-old mice.Furthermore,both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-,4-,and 5-week-old mice.In summary,the results of this study suggested that B.longum subsp.infantis CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner.展开更多
Bifidobacterium longum subsp.infantis(B.infantis)is the most active consumer of human milk oligosaccharides(HMOs),which can promote the development and maturation of the infant’s intestinal immune system.In this stud...Bifidobacterium longum subsp.infantis(B.infantis)is the most active consumer of human milk oligosaccharides(HMOs),which can promote the development and maturation of the infant’s intestinal immune system.In this study,we collected information on B.infantis isolated from human feces in the NCBI database to analyze their whole genome.We found that the whole genome of the tested strains and the functional genes utilizing the HMOs showed geographical clustering.Comparison of the genes encodingα-L-fucosidase between B.infantis H11 and BINF revealed that strain H11 had moreα-L-fucosidase genes,and further heterologous expression ofα-Lfucosidase showed that only the glycoside hydrolase(GH)95 family could hydrolyze 2’-fucosyllactose(2’-FL).At the same time,FUC95A(derived from strain H11)was more efficient in catalyzing 2’-FL than FUC95B(derived from strain BINF).In addition,metabolites in the 2’-FL fermentation supernatants were analyzed based on untargeted metabolomics,and it was found that strain H11 could utilize 2’-FL to produce more beneficial metabolites compared to strain BINF.In conclusion,we hypothesized that the enhancement ofα-L-fucosidase activity of B.infantis is one of the essential requirements to improve the utilization of 2’-FL and increase the contents of beneficial metabolites to perform the probiotic function.展开更多
Although all strains of Bifidobacterium longum subsp.infantis(B.infantis)can utilize 2′-fucosyllactose(2′-FL)as a carbon source,they exhibit notable strain-level differences in utilization efficiency and genetic mec...Although all strains of Bifidobacterium longum subsp.infantis(B.infantis)can utilize 2′-fucosyllactose(2′-FL)as a carbon source,they exhibit notable strain-level differences in utilization efficiency and genetic mechanisms.To investigate these intra-subspecific variations,we compared 2′-FL metabolism in B.infantis Y46 and the type strain B.infantis ATCC 15697 using phenotypic assays and whole-genome sequencing.Both strains were able to grow on 2′-FL as the sole carbon source,although Y46 demonstrated markedly higher growth and metabolic activity than ATCC 15697.Genomic analysis revealed Y46 has more carbohydrate metabolism genes,including 10 carbohydrate-active enzyme(CAZyme)genes absent in ATCC 15697.Despite lacking the 2′-FL transporter gene FL1_SBP,Y46 showed significantly upregulated expression of FL2_SBP,another 2′-FL transporters,and enhanced 2′-FL metabolism,suggesting an adaptive mechanism that compensates for FL1_SBP loss.These findings highlight the metabolic flexibility and ecological adaptability of Y46 and provide insight into the genomic basis of strain-specific 2′-FL utilization,offering a reference for future probiotic development.展开更多
The early life period is a critical phase for the colonization of initial gut microbes in infants,during which the immune system develops and matures.These processes have long-lasting implications that may extend into...The early life period is a critical phase for the colonization of initial gut microbes in infants,during which the immune system develops and matures.These processes have long-lasting implications that may extend into adolescence and even adulthood.Bifidobacterium longum subsp.infantis,recognized as an ideal probiotic during infancy,has the potential to modulate intestinal immunity and mitigate immune-mediated diseases.This research explored the potential of various B.longum subsp.infantis strains to modulate the balance between T helper(Th)1 and Th2 responses,immune cell populations,Immunoglobulin A(IgA),and the related genes expression in mice.Notably,B.longum subsp.infantis FHNFQ4M11 and CCFM1269 demonstrated more robust regulatory capabilities.These two strains,which exhibited higher ILA production,activated the aryl hydrocarbon receptor(AHR)signaling pathway,upregulated the levels of galectin-1(Gal-1)and galectin-3(Gal-3),and modulated Th1/Th2 differentiation markers in the colon.Additionally,these strains significantly elevated IgA concentrations in both serum and colon and modulated the gut microbiota composition.These findings underscored the potential of specific B.longum subsp.infantis strains as targeted probiotic interventions in early life,providing a promising strategy for promoting immune homeostasis and gut health in infants.展开更多
Human milk oligosaccharides(HMOs)are considered the“golden ingredients”of human milk,and Bifidobacte-rium longum subsp.infantis(B.infantis)is not only indigenous probiotics of the infant gut,but also efficiently uti...Human milk oligosaccharides(HMOs)are considered the“golden ingredients”of human milk,and Bifidobacte-rium longum subsp.infantis(B.infantis)is not only indigenous probiotics of the infant gut,but also efficiently utilizes HMOs.The aim of this study was to investigate the potential manners in which B.infantis interacts with HMOs to promote intestinal health.We found that all strains of B.infantis tested had varying degrees of pro-tective effects on the cellular barrier,with B.infantis H11 being the most effective.The whole genome results of strain H11 revealed that it was enriched in genes related to HMOs consumption,and it could efficiently utilize HMOs,especially fully utilizing 2′-FL,3-FL,and DFL,utilizing about 60%of 3′-SL and 6′-SL,while the utilization of LNnT was lowest.By performing untargeted metabolomics analyses on the fermentation supernatant of strain H11 that utilized HMOs or lactose as a carbon source,it was found that strain H11 could utilize both HMOs and lactose to produce indole-3-lactic acid,indoleacetic acid,and other substances with potential probiotic functions.Furthermore,the tryptophan metabolic pathway was enriched with a variety of significantly different metabo-lites and had a large impact on the metabolic process,especially the serotonin pathway branch.In the fermentation supernatant of strain H11 utilizing HMOs as a carbon source,there was a significant increase in the levels of beneficial metabolites in the serotonin pathway.This may be one of the essential manners for the utilization of HMOs by strain H11 to exert probiotic potential.展开更多
Objectives:Constipation is a prevalent gastrointestinal issue,and the efficacy of probiotics in alleviating constipation has been well demonstrated.This study aimed to investigate the impact of Bifidobacterium longum ...Objectives:Constipation is a prevalent gastrointestinal issue,and the efficacy of probiotics in alleviating constipation has been well demonstrated.This study aimed to investigate the impact of Bifidobacterium longum subsp.infantis NKU FB3-14 on loperamide-induced constipation by focusing on improving intestinal barrier function and modulating gut microbiota composition.Materials and Methods:The constipated model mice induced by loperamide were treated with NKU FB3-14,and the laxative effect was assessed based on fecal water content,first black stool time and gastrointestinal transit rate.Gastrointestinal regulatory peptides in serum and intestinal neurotransmitter and inflammatory cytokines in colon tissues were measured using enzyme-linked immunosorbent assay kits.Changes in the composition of gut microbiota were analyzed through 16S ribosomal DNA(rDNA)sequencing.Additionally,high-performance liquid chromatography(HPLC)was performed to quantify levels of short-chain fatty acids(SCFAs)in feces.Results:Treatment with NKU FB3-14 increased fecal water content,shortened the first black stool time,and improved the small intestine transit rate.Motilin and substance-P significantly decreased in the model group,and only motilin increased in the FB3-14 group;somatostatin and vasoactive intestinal peptide were decreased in the model mice and both increased in the FB3-14 group;5-hydroxytryptamine(5-HT)levels in the colon tissue were upregulated following NKU FB3-14 treatment.Histological examination revealed thinner colonic mucosa in the model group along with significant increases in tumor necrosis factorα(TNF-α),interleukin 1β(IL-1β),and interleukin 17(IL-17)levels in the colon tissues,which were alleviated by NKU FB 3-14 treatment.Furthermore,NKU FB3-14 intervention resulted in reduced abundance of Desulfobacterota and Desulfovibrio while increasing the abundance of Ruminococcaceae and Eubacterium;a higher level of butyric acid was observed in feces.Conclusions:In summary,our findings demonstrated that NKU FB3-14 treatment significantly enhanced intestinal motility,regulated the expression levels of gastrointestinal regulatory peptides,prevented damage to colonic barriers,and ameliorated gut microbiota imbalance associated with loperamide-induced constipation.展开更多
Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by g...Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by genetic factors but also by en-vironmental factors,particularly the symbiotic microbiota.Bifidobacterium animalis subsp.lactis(BB-12),originally found in dairy products,is widely used in infant for-mula and dietary supplements.However,its role and mechanisms in immune develop-ment during early life remain unclear.Methods:Using GF mice as the experimental model,B.animalis subsp.lactis BB-12 was administered via gavage during early life.In the juvenile stage,changes in T-cell subsets in the spleen,thymus,and gut intraepithelial lymphocytes(IEL)were assessed using spectral flow cytometry.Additionally,targeted metabolomics analysis of trypto-phan metabolism and short-chain fatty acid pathways in colonic tissue was conducted to explore how B.animalis subsp.lactis BB-12 influences the immune system through gut microbiota metabolism.Results:BB-12 effectively modulates the gut immune microenvironment,leading to beneficial changes in T-cell subsets in key immune tissues such as the spleen,thymus,and gut IELs.Metabolomics analysis further supports these findings by showing that BB-12 intervention greatly increased the production of tryptophan derivatives and acetic acid in the colon of GF mice.Conclusion:The findings provide theoretical evidence for the role of B.animalis subsp.lactis in immune system development and support its application in dietary supple-ments,suggesting potential as a component for infant immune health and in prevent-ing immune-related diseases.展开更多
1)Bifidobacterium longum subsp.iuvenis(Modesto et al.IJSEM.2023,73:6013;Type strain:NCC 5000 T=CCOS 2034 T=LMG 32752^(T))Etymology:iu’ve.nis.L.gen.masc./fem.n.iuvenis,of child,because the type strain was isolated fro...1)Bifidobacterium longum subsp.iuvenis(Modesto et al.IJSEM.2023,73:6013;Type strain:NCC 5000 T=CCOS 2034 T=LMG 32752^(T))Etymology:iu’ve.nis.L.gen.masc./fem.n.iuvenis,of child,because the type strain was isolated from a 2 year old Bangladeshi child.长双歧杆菌幼儿亚种.展开更多
Global seafood demand has continued to rise amidst challenges to traditional aquaculture operations.Current shrimp aquaculture practice requires high water exchange and discharges toxic effluent to the environment.Bio...Global seafood demand has continued to rise amidst challenges to traditional aquaculture operations.Current shrimp aquaculture practice requires high water exchange and discharges toxic effluent to the environment.Biofloc technology(BFT)is self-sustaining and emphasizes nutrient cycling through microbial activity to maintain water quality.The effect of BFT on water quality and profitability of shrimp(Litopenaeus vannamei)culture was determined over a 70-day period.Ponds P1,P2,and P3 were treated with BFT and compared to a control group(P4)without BFT.Bacillus infantis cultured inoculum initiated biofloc development while molasses-maintained C:N ratio of 15:1.One-way ANOVA determined the mean differences in Temperature,pH,dissolved oxygen(DO),total dissolved solid,alkalinity,salinity,ammonia(NH_(3)),nitrates,nitrites(NO_(2)^(-)),calcium,magnesium,as well as shrimp body weight(BW)and total length(TL)across treatments.Profitability was determined by comparing the cost of production with sales and plotting it on a bar chart.BFT shrimp exhibited significantly higher BW(13.6 g)compared to 8.1 g in the control,and maintained a higher survival rate(80–90%)by day 70.Water quality was better managed in BFT,with NH_(3) consistently kept below 0.5mg/L;transient peaks of NO_(2)^(-),more stable pH(averaging at 7.5),and better DO management,maintained above 5 mg/L.BFT provided higher profitability of Ringgit Malaysia(RM)11,019.67(P1)and RM 8651.83(P2)compared to financiallosses in the non-biofloc system.Although operational challenges were reported,BFT showed superior resilience,suggesting that proper technical training and farm management are crucial for its optimization.展开更多
Bacterial soft rot(BSR)caused by Pectobacterium carotovorum subsp.brasiliense(Pcb)is a serious bacterial disease which negatively impact yield and quality in cucumber.However,the genetic mechanism of BSR resistance in...Bacterial soft rot(BSR)caused by Pectobacterium carotovorum subsp.brasiliense(Pcb)is a serious bacterial disease which negatively impact yield and quality in cucumber.However,the genetic mechanism of BSR resistance in cucumber has not been reported.Here,we investigated the BSR resistance of 119 cucumber core germplasm worldwide at the seedling stage and identified 26 accessions highly resistant to BSR.A total of 1642740 single-nucleotide polymorphisms(SNPs)were used to conduct GWAS,and five loci associated with BSR resistance were detected on four chromosomes:gBSR2.1,gBSR2.2,gBSR3.1,gBSR4.1 and gBSR5.1.Based on haplotype analysis,sequence polymorphisms,functional annotation and qRT-PCR analysis,six candidate genes were identified within the five loci.CsaV3_2G014450,CsaV3_2G014490,CsaV3_2G016000,CsaV3_3G000850,CsaV3_4G033150,and CsaV3_5G000390 each had nonsynonymous SNPs,and were significantly up-regulated in the resistant genotypes after inoculation.And CsaV3_5G000390 in the susceptible genotype was significantly up-regulated after inoculation.The identification of these candidate genes lays a foundation for understanding the genetic mechanism of BSR resistance in cucumber.Generally,our study mined genes associated with BSR resistance in cucumber seedlings and will assist the breeding of BSR-resistant cucumber cultivars.展开更多
Accumulating evidence suggests that probiotics affect the microbial-gut-brain axis in a way that can prevent and treat Alzheimer's disease(AD).However,the mechanism underlying the therapeutic effects still needs t...Accumulating evidence suggests that probiotics affect the microbial-gut-brain axis in a way that can prevent and treat Alzheimer's disease(AD).However,the mechanism underlying the therapeutic effects still needs to be further investigated.This study aimed to examine the alleviating effect of Lacticaseibacillus paracasei L9 and Bifidobacterium animalis subsp.lactis A6 and possible mechanism in mice with AD.In this study,the administration of probiotics L9 and A6 effectively improved memory and learning functions in AD mice.The hippocampal cells of mice were partly recovered in morphology and rearranged more neatly after probiotics intervention.Meanwhile,L9 and A6 exhibited inhibitory effects on the phosphorylation of Tau and the deposition of Aβ,which were mediated via GSK-3βand PP2A kinases.Meanwhile,by metagenomic sequencing,we found interventions with L9 and A6 altered the intestinal microbiome's taxonomic composition,reduced the abundance of pathogenic Desulfovibrio genera,and increased beneficial Clostridium and Paramuribaculum genera abundance.The fatty acids metabolism and biosynthesis of gut microbiome were also enhanced.Serum untargeted metabolomics also showed noticeable alternation in lipidrelated metabolites,which may alleviate the pathogenesis of AD.These results revealed a mitigating role for probiotic L9 and A6 in AD prevention and offer new insights into AD prevention via gut-brain connection.展开更多
Microbial food is an important direction of sustainable food development in the future.Microorganisms such as lactic acid bacteria(LAB)are important sources of natural radioprotectors.Moderate environmental stress can...Microbial food is an important direction of sustainable food development in the future.Microorganisms such as lactic acid bacteria(LAB)are important sources of natural radioprotectors.Moderate environmental stress can induce stress response and improve biological activity of LAB.In the previous study,Lactococcus lactis subsp.lactis IL1403(L.lactis IL1403)cell-free extract induced by ionizing radiation(IR)of 500 Gy(IR-CFE)presented the stronger radioprotective effect than untreated cell-free extract in mice.To explore the radioprotective active substances of IR-CFE,the key protein was screened by proteomics and its radioprotective effect in vivo was further evaluated.The Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment showed that two-component systems(TCS)were enhanced to adapt the IR induction.Meanwhile,the protein LlrG belonged to TCS was screened by the protein-protein interaction.Furthermore,the recombinant protein LlrG(rLlrG)could markedly alleviate the ^(60)Coγ-induced damage to the hematopoietic system,oxidative stress and inflammation in mice,thereby exerting its radioprotective function.These results suggested LlrG protein not only played an important role in the adaptation of L.lactis IL1403 to IR environment,but also exerted a good radioprotective effect,which could be applied in the development of protein-based radioprotectors.展开更多
【目的】分离鉴定江苏省扬州市养殖场异育银鲫患病病原。【方法】采用常规的理化特性和分子生物学的方法,对从濒死异育银鲫肝脏处分离到的菌株YZ-1进行表型生物学、分子生物学及药敏试验的系统研究。【结果】该菌株16S r RNA基因(序列长...【目的】分离鉴定江苏省扬州市养殖场异育银鲫患病病原。【方法】采用常规的理化特性和分子生物学的方法,对从濒死异育银鲫肝脏处分离到的菌株YZ-1进行表型生物学、分子生物学及药敏试验的系统研究。【结果】该菌株16S r RNA基因(序列长度1 446 bp,Gen Bank登录号为JX164202)与其它杀鲑气单胞菌16S r RNA基因一致性在99%-100%之间,构建发育树确定该菌株为杀鲑气单胞菌杀鲑亚种(Aeromonas salmonicida subsp.salmonicida)。人工回感可导致异育银鲫死亡。药敏试验结果显示:对头孢呋辛、复方新诺明、恩诺沙星等23种抗生素敏感;对阿米卡星、四环素、大观霉素、头孢拉定等11种抗生素中度敏感;对青霉素G、链霉素、庆大霉素、氟苯尼考、万古霉素等10种抗生素耐药。【结论】研究结果证实引起异育银鲫死亡的病原为杀鲑气单胞菌杀鲑亚种。展开更多
基金supported by the National Natural Science Foundation of China(32021005,32272332)the Fundamental Research Funds for the Central Universities(JUSRP622020,JUSRP51501)+1 种基金the Program of Collaborative Innovation Centre of Food Safety and Quality Control in Jiangsu ProvincePostgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX22_2391)。
文摘Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effects of these 2 bifidobacterial species against Salmonella infection.The results demonstrated that B.longum subsp.infantis was more effective than B.adolescentis in alleviating the severity of infection in newborn rats exposed to Salmonella enterica serovar Typhimurium strain SL1344.B.longum subsp.infantis attenuated intestinal inflammation and mucosal damage induced by Salmonella infection,as well as protecting intestinal nerves and intestinal barrier function through TLR4/My D88 signalling.B.longum subsp.infantis also displayed the potential to modulate gut metabolites by promoting the biosynthesis of unsaturated fatty acids(arachidonic acid,eicosapentaenoic acid andα-linolenic acid)and purine metabolism(guanine,adenine,inosine and adenosine),thereby regulating metabolic disturbances.Additionally,the benefits of B.longum subsp.infantis were also observed in the liver,spleen and brain,improving nerve reflexes and suppressing hepatosplenomegaly.Overall,these findings provide novel insights into the prevention and treatment of gutrelated diseases in newborns,highlighting the potentially significant role of B.longum subsp.infantis in clinical applications.
基金funded by the National Key R&D Program of China(2021YFD2100700)National Natural Science Foundation of China(32021005)+1 种基金111 project(BP0719028)Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province。
文摘Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to explore the effects of B.longum subsp.infantis supplementation on interferon-beta(IFN-β)secretion and intestinal barrier improvement in growing mice.Female and male mice were orally administered either saline or B.longum subsp.infantis CCFM1269 or I5TI(1×10^(9) CFU/mice per day,n=8)from 1-week-age until 3-,4-,and 5-week-age.RNA sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2'-5'oligoadenylate synthetase(OAS).Additionally,CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling pathway.However,this effect was not observed in 4-and 5-week-old mice.Furthermore,both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-,4-,and 5-week-old mice.In summary,the results of this study suggested that B.longum subsp.infantis CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner.
基金supported financially by the National Natural Science Foundation of China(No.32272291)Heilongjiang Province Key Research and Development Program(Innovation Base)(No.JD2023SJ15).
文摘Bifidobacterium longum subsp.infantis(B.infantis)is the most active consumer of human milk oligosaccharides(HMOs),which can promote the development and maturation of the infant’s intestinal immune system.In this study,we collected information on B.infantis isolated from human feces in the NCBI database to analyze their whole genome.We found that the whole genome of the tested strains and the functional genes utilizing the HMOs showed geographical clustering.Comparison of the genes encodingα-L-fucosidase between B.infantis H11 and BINF revealed that strain H11 had moreα-L-fucosidase genes,and further heterologous expression ofα-Lfucosidase showed that only the glycoside hydrolase(GH)95 family could hydrolyze 2’-fucosyllactose(2’-FL).At the same time,FUC95A(derived from strain H11)was more efficient in catalyzing 2’-FL than FUC95B(derived from strain BINF).In addition,metabolites in the 2’-FL fermentation supernatants were analyzed based on untargeted metabolomics,and it was found that strain H11 could utilize 2’-FL to produce more beneficial metabolites compared to strain BINF.In conclusion,we hypothesized that the enhancement ofα-L-fucosidase activity of B.infantis is one of the essential requirements to improve the utilization of 2’-FL and increase the contents of beneficial metabolites to perform the probiotic function.
基金supported by National Key R&D Program of China(2024YFD2100900).
文摘Although all strains of Bifidobacterium longum subsp.infantis(B.infantis)can utilize 2′-fucosyllactose(2′-FL)as a carbon source,they exhibit notable strain-level differences in utilization efficiency and genetic mechanisms.To investigate these intra-subspecific variations,we compared 2′-FL metabolism in B.infantis Y46 and the type strain B.infantis ATCC 15697 using phenotypic assays and whole-genome sequencing.Both strains were able to grow on 2′-FL as the sole carbon source,although Y46 demonstrated markedly higher growth and metabolic activity than ATCC 15697.Genomic analysis revealed Y46 has more carbohydrate metabolism genes,including 10 carbohydrate-active enzyme(CAZyme)genes absent in ATCC 15697.Despite lacking the 2′-FL transporter gene FL1_SBP,Y46 showed significantly upregulated expression of FL2_SBP,another 2′-FL transporters,and enhanced 2′-FL metabolism,suggesting an adaptive mechanism that compensates for FL1_SBP loss.These findings highlight the metabolic flexibility and ecological adaptability of Y46 and provide insight into the genomic basis of strain-specific 2′-FL utilization,offering a reference for future probiotic development.
基金supported by the National Natural Science Foundation of China(32394051)the Fundamental Research Funds for the Central Universitiesthe Collaborative Innovation Centre of Food Safety and Quality Control in Jiangsu Province.
文摘The early life period is a critical phase for the colonization of initial gut microbes in infants,during which the immune system develops and matures.These processes have long-lasting implications that may extend into adolescence and even adulthood.Bifidobacterium longum subsp.infantis,recognized as an ideal probiotic during infancy,has the potential to modulate intestinal immunity and mitigate immune-mediated diseases.This research explored the potential of various B.longum subsp.infantis strains to modulate the balance between T helper(Th)1 and Th2 responses,immune cell populations,Immunoglobulin A(IgA),and the related genes expression in mice.Notably,B.longum subsp.infantis FHNFQ4M11 and CCFM1269 demonstrated more robust regulatory capabilities.These two strains,which exhibited higher ILA production,activated the aryl hydrocarbon receptor(AHR)signaling pathway,upregulated the levels of galectin-1(Gal-1)and galectin-3(Gal-3),and modulated Th1/Th2 differentiation markers in the colon.Additionally,these strains significantly elevated IgA concentrations in both serum and colon and modulated the gut microbiota composition.These findings underscored the potential of specific B.longum subsp.infantis strains as targeted probiotic interventions in early life,providing a promising strategy for promoting immune homeostasis and gut health in infants.
基金supported financially by the National Natural Science Foundation of China(No.32272291)Heilongjiang Province Key Research and Development Program(Innovation Base)(No.JD2023SJ15).
文摘Human milk oligosaccharides(HMOs)are considered the“golden ingredients”of human milk,and Bifidobacte-rium longum subsp.infantis(B.infantis)is not only indigenous probiotics of the infant gut,but also efficiently utilizes HMOs.The aim of this study was to investigate the potential manners in which B.infantis interacts with HMOs to promote intestinal health.We found that all strains of B.infantis tested had varying degrees of pro-tective effects on the cellular barrier,with B.infantis H11 being the most effective.The whole genome results of strain H11 revealed that it was enriched in genes related to HMOs consumption,and it could efficiently utilize HMOs,especially fully utilizing 2′-FL,3-FL,and DFL,utilizing about 60%of 3′-SL and 6′-SL,while the utilization of LNnT was lowest.By performing untargeted metabolomics analyses on the fermentation supernatant of strain H11 that utilized HMOs or lactose as a carbon source,it was found that strain H11 could utilize both HMOs and lactose to produce indole-3-lactic acid,indoleacetic acid,and other substances with potential probiotic functions.Furthermore,the tryptophan metabolic pathway was enriched with a variety of significantly different metabo-lites and had a large impact on the metabolic process,especially the serotonin pathway branch.In the fermentation supernatant of strain H11 utilizing HMOs as a carbon source,there was a significant increase in the levels of beneficial metabolites in the serotonin pathway.This may be one of the essential manners for the utilization of HMOs by strain H11 to exert probiotic potential.
基金funded by the Fundamental Research Funds for the Central Universities,Nankai University,China(Nos.63241340 and 63241341).
文摘Objectives:Constipation is a prevalent gastrointestinal issue,and the efficacy of probiotics in alleviating constipation has been well demonstrated.This study aimed to investigate the impact of Bifidobacterium longum subsp.infantis NKU FB3-14 on loperamide-induced constipation by focusing on improving intestinal barrier function and modulating gut microbiota composition.Materials and Methods:The constipated model mice induced by loperamide were treated with NKU FB3-14,and the laxative effect was assessed based on fecal water content,first black stool time and gastrointestinal transit rate.Gastrointestinal regulatory peptides in serum and intestinal neurotransmitter and inflammatory cytokines in colon tissues were measured using enzyme-linked immunosorbent assay kits.Changes in the composition of gut microbiota were analyzed through 16S ribosomal DNA(rDNA)sequencing.Additionally,high-performance liquid chromatography(HPLC)was performed to quantify levels of short-chain fatty acids(SCFAs)in feces.Results:Treatment with NKU FB3-14 increased fecal water content,shortened the first black stool time,and improved the small intestine transit rate.Motilin and substance-P significantly decreased in the model group,and only motilin increased in the FB3-14 group;somatostatin and vasoactive intestinal peptide were decreased in the model mice and both increased in the FB3-14 group;5-hydroxytryptamine(5-HT)levels in the colon tissue were upregulated following NKU FB3-14 treatment.Histological examination revealed thinner colonic mucosa in the model group along with significant increases in tumor necrosis factorα(TNF-α),interleukin 1β(IL-1β),and interleukin 17(IL-17)levels in the colon tissues,which were alleviated by NKU FB 3-14 treatment.Furthermore,NKU FB3-14 intervention resulted in reduced abundance of Desulfobacterota and Desulfovibrio while increasing the abundance of Ruminococcaceae and Eubacterium;a higher level of butyric acid was observed in feces.Conclusions:In summary,our findings demonstrated that NKU FB3-14 treatment significantly enhanced intestinal motility,regulated the expression levels of gastrointestinal regulatory peptides,prevented damage to colonic barriers,and ameliorated gut microbiota imbalance associated with loperamide-induced constipation.
基金supported by the National Key R&D Programs of China(2022YFF0710701,2022YFF0710702)Guangzhou Key Research and Development Program(202206010157)+2 种基金Guangzhou Joint Fund for Key Laboratory(202201020381)Medical Joint Fund of Jinan University(YXJC202204)Open Research Project of the Key Laboratory of Viral Pathogenesis and Infection Prevention and Control of the Ministry of Education(2023VPPC-R02).
文摘Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by genetic factors but also by en-vironmental factors,particularly the symbiotic microbiota.Bifidobacterium animalis subsp.lactis(BB-12),originally found in dairy products,is widely used in infant for-mula and dietary supplements.However,its role and mechanisms in immune develop-ment during early life remain unclear.Methods:Using GF mice as the experimental model,B.animalis subsp.lactis BB-12 was administered via gavage during early life.In the juvenile stage,changes in T-cell subsets in the spleen,thymus,and gut intraepithelial lymphocytes(IEL)were assessed using spectral flow cytometry.Additionally,targeted metabolomics analysis of trypto-phan metabolism and short-chain fatty acid pathways in colonic tissue was conducted to explore how B.animalis subsp.lactis BB-12 influences the immune system through gut microbiota metabolism.Results:BB-12 effectively modulates the gut immune microenvironment,leading to beneficial changes in T-cell subsets in key immune tissues such as the spleen,thymus,and gut IELs.Metabolomics analysis further supports these findings by showing that BB-12 intervention greatly increased the production of tryptophan derivatives and acetic acid in the colon of GF mice.Conclusion:The findings provide theoretical evidence for the role of B.animalis subsp.lactis in immune system development and support its application in dietary supple-ments,suggesting potential as a component for infant immune health and in prevent-ing immune-related diseases.
文摘1)Bifidobacterium longum subsp.iuvenis(Modesto et al.IJSEM.2023,73:6013;Type strain:NCC 5000 T=CCOS 2034 T=LMG 32752^(T))Etymology:iu’ve.nis.L.gen.masc./fem.n.iuvenis,of child,because the type strain was isolated from a 2 year old Bangladeshi child.长双歧杆菌幼儿亚种.
基金supported by Ministry of Higher Education,Malaysia through the Translational Research Program entitled Program Traslasional Sektor Makanan Laut Malaysia untuk Kelestarian Pengeluaran Udang Marin dan Penjanaan Pendapatan-Tinggi:Aplikasi Teknologi“Rapid Biofloc”(UMT/PPIJIM/2-2/68/Trans-KPT)grant number[Vot.No:58932].
文摘Global seafood demand has continued to rise amidst challenges to traditional aquaculture operations.Current shrimp aquaculture practice requires high water exchange and discharges toxic effluent to the environment.Biofloc technology(BFT)is self-sustaining and emphasizes nutrient cycling through microbial activity to maintain water quality.The effect of BFT on water quality and profitability of shrimp(Litopenaeus vannamei)culture was determined over a 70-day period.Ponds P1,P2,and P3 were treated with BFT and compared to a control group(P4)without BFT.Bacillus infantis cultured inoculum initiated biofloc development while molasses-maintained C:N ratio of 15:1.One-way ANOVA determined the mean differences in Temperature,pH,dissolved oxygen(DO),total dissolved solid,alkalinity,salinity,ammonia(NH_(3)),nitrates,nitrites(NO_(2)^(-)),calcium,magnesium,as well as shrimp body weight(BW)and total length(TL)across treatments.Profitability was determined by comparing the cost of production with sales and plotting it on a bar chart.BFT shrimp exhibited significantly higher BW(13.6 g)compared to 8.1 g in the control,and maintained a higher survival rate(80–90%)by day 70.Water quality was better managed in BFT,with NH_(3) consistently kept below 0.5mg/L;transient peaks of NO_(2)^(-),more stable pH(averaging at 7.5),and better DO management,maintained above 5 mg/L.BFT provided higher profitability of Ringgit Malaysia(RM)11,019.67(P1)and RM 8651.83(P2)compared to financiallosses in the non-biofloc system.Although operational challenges were reported,BFT showed superior resilience,suggesting that proper technical training and farm management are crucial for its optimization.
基金supported by the National Key Research and Development Program of China(Grant No.2023YFD1200101)the Earmarked Fund for Modern Agro-industry Technology Research System(Grant No.CARS-23)Science and Technology Innovation Program of the Chinese Academy of Agricultural Science(Grant No.CAAS-ASTIP-IVFCAAS).
文摘Bacterial soft rot(BSR)caused by Pectobacterium carotovorum subsp.brasiliense(Pcb)is a serious bacterial disease which negatively impact yield and quality in cucumber.However,the genetic mechanism of BSR resistance in cucumber has not been reported.Here,we investigated the BSR resistance of 119 cucumber core germplasm worldwide at the seedling stage and identified 26 accessions highly resistant to BSR.A total of 1642740 single-nucleotide polymorphisms(SNPs)were used to conduct GWAS,and five loci associated with BSR resistance were detected on four chromosomes:gBSR2.1,gBSR2.2,gBSR3.1,gBSR4.1 and gBSR5.1.Based on haplotype analysis,sequence polymorphisms,functional annotation and qRT-PCR analysis,six candidate genes were identified within the five loci.CsaV3_2G014450,CsaV3_2G014490,CsaV3_2G016000,CsaV3_3G000850,CsaV3_4G033150,and CsaV3_5G000390 each had nonsynonymous SNPs,and were significantly up-regulated in the resistant genotypes after inoculation.And CsaV3_5G000390 in the susceptible genotype was significantly up-regulated after inoculation.The identification of these candidate genes lays a foundation for understanding the genetic mechanism of BSR resistance in cucumber.Generally,our study mined genes associated with BSR resistance in cucumber seedlings and will assist the breeding of BSR-resistant cucumber cultivars.
基金funded by the National Key R&D Program of China(2023YFF1104501)R&D Program of Beijing Municipal Education Commission(23JF0006)National Natural Science Foundation of China(31601443 and 32101938)。
文摘Accumulating evidence suggests that probiotics affect the microbial-gut-brain axis in a way that can prevent and treat Alzheimer's disease(AD).However,the mechanism underlying the therapeutic effects still needs to be further investigated.This study aimed to examine the alleviating effect of Lacticaseibacillus paracasei L9 and Bifidobacterium animalis subsp.lactis A6 and possible mechanism in mice with AD.In this study,the administration of probiotics L9 and A6 effectively improved memory and learning functions in AD mice.The hippocampal cells of mice were partly recovered in morphology and rearranged more neatly after probiotics intervention.Meanwhile,L9 and A6 exhibited inhibitory effects on the phosphorylation of Tau and the deposition of Aβ,which were mediated via GSK-3βand PP2A kinases.Meanwhile,by metagenomic sequencing,we found interventions with L9 and A6 altered the intestinal microbiome's taxonomic composition,reduced the abundance of pathogenic Desulfovibrio genera,and increased beneficial Clostridium and Paramuribaculum genera abundance.The fatty acids metabolism and biosynthesis of gut microbiome were also enhanced.Serum untargeted metabolomics also showed noticeable alternation in lipidrelated metabolites,which may alleviate the pathogenesis of AD.These results revealed a mitigating role for probiotic L9 and A6 in AD prevention and offer new insights into AD prevention via gut-brain connection.
基金supported by the National Natural Science Foundation of China(32172211,32572557)the Key Science Research Projects of Higher Education Institutions in Henan Province(21A530006)+3 种基金the Key Research and Development and Promotion Projects of Henan Province(232102310293)the Henan Provincial Natural Science Foundation General Program(252300421403)the Zhongyuan Sci-Tech Innovation Leading Talents(254200510040)the Innovative Research Team(in Science and Technology)in University of Henan Province(26IRTSTHN030).
文摘Microbial food is an important direction of sustainable food development in the future.Microorganisms such as lactic acid bacteria(LAB)are important sources of natural radioprotectors.Moderate environmental stress can induce stress response and improve biological activity of LAB.In the previous study,Lactococcus lactis subsp.lactis IL1403(L.lactis IL1403)cell-free extract induced by ionizing radiation(IR)of 500 Gy(IR-CFE)presented the stronger radioprotective effect than untreated cell-free extract in mice.To explore the radioprotective active substances of IR-CFE,the key protein was screened by proteomics and its radioprotective effect in vivo was further evaluated.The Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment showed that two-component systems(TCS)were enhanced to adapt the IR induction.Meanwhile,the protein LlrG belonged to TCS was screened by the protein-protein interaction.Furthermore,the recombinant protein LlrG(rLlrG)could markedly alleviate the ^(60)Coγ-induced damage to the hematopoietic system,oxidative stress and inflammation in mice,thereby exerting its radioprotective function.These results suggested LlrG protein not only played an important role in the adaptation of L.lactis IL1403 to IR environment,but also exerted a good radioprotective effect,which could be applied in the development of protein-based radioprotectors.
文摘【目的】分离鉴定江苏省扬州市养殖场异育银鲫患病病原。【方法】采用常规的理化特性和分子生物学的方法,对从濒死异育银鲫肝脏处分离到的菌株YZ-1进行表型生物学、分子生物学及药敏试验的系统研究。【结果】该菌株16S r RNA基因(序列长度1 446 bp,Gen Bank登录号为JX164202)与其它杀鲑气单胞菌16S r RNA基因一致性在99%-100%之间,构建发育树确定该菌株为杀鲑气单胞菌杀鲑亚种(Aeromonas salmonicida subsp.salmonicida)。人工回感可导致异育银鲫死亡。药敏试验结果显示:对头孢呋辛、复方新诺明、恩诺沙星等23种抗生素敏感;对阿米卡星、四环素、大观霉素、头孢拉定等11种抗生素中度敏感;对青霉素G、链霉素、庆大霉素、氟苯尼考、万古霉素等10种抗生素耐药。【结论】研究结果证实引起异育银鲫死亡的病原为杀鲑气单胞菌杀鲑亚种。
