Endoscopic submucosal dissection(ESD)is the standard treatment for early-stage gastric cancer,but the large post-operative ulcers caused by ESD often lead to serious side effects.Post-ESD mu-cosal repair materials pro...Endoscopic submucosal dissection(ESD)is the standard treatment for early-stage gastric cancer,but the large post-operative ulcers caused by ESD often lead to serious side effects.Post-ESD mu-cosal repair materials provide a new option for the treatment of post-ESD ulcers.In this study,we developed a polyurethane/small intestinal submucosa(PU/SIS)hydrogel and investigated its effi-cacy for accelerating ESD-induced ulcer healing in a canine model.PU/SIS hydrogel possessed great biocompatibility and distinctive pH-sensitive swelling properties and protected GES-1 cells from acid attack through forming a dense film in acidic conditions in vitro.Besides,PU/SIS gels present a strong bio-adhesion to gastric tissues under acidic conditions,thus ensuring the reten-tion time of PU/SIS gels in vivo.In a canine model,PU/SIS hydrogel was easily delivered via endoscopy and adhered to the ulcer sites.PU/SIS hydrogel accelerated gastric ulcer healing at an early stage with more epithelium regeneration and slight inflammation.Our findings reveal PU/SIS hydrogel is a promising and attractive candidate for ESD-induced ulcer repair.展开更多
目的采用高通量测序技术检测溃疡性结肠炎(ulcerative colitis,UC)和正常人群的肠道菌群分布,挖掘与UC形成和发展显著相关的菌属结构,促进UC的研究和新药开发.方法提取各研究样本染色体DNA,并进行DNA片段长度检测和定量.对提取的DNA样...目的采用高通量测序技术检测溃疡性结肠炎(ulcerative colitis,UC)和正常人群的肠道菌群分布,挖掘与UC形成和发展显著相关的菌属结构,促进UC的研究和新药开发.方法提取各研究样本染色体DNA,并进行DNA片段长度检测和定量.对提取的DNA样本进行16S r RNA V3区基因扩增及焦磷酸测序.结果 U C患者的肠道菌群整体结构与健康对照人群具有显著的差异,主要表现为拟杆菌门、变形菌门和厚壁菌门丰度降低.其中在11个代谢通路中二组平均丰度存在显著差异(P<0.05).结论肠道菌群结构的异常改变与UC的发生发展存在较强相关性.多个代谢通路参与了UC的形成发展.通过分析疾病相关的关键菌群结构,有助于了解疾病的分子病理基础,并为新药设计提供可借鉴的理论依据.展开更多
基金This work was supported by The National Key R&D Program of China(2017YFC1104702)Sichuan Science and Technology Program(2019JDRC0020)the 1.3.5 Project for Disciplines of Excellence,West China Hospital,Sichuan University(ZYJC18002).
文摘Endoscopic submucosal dissection(ESD)is the standard treatment for early-stage gastric cancer,but the large post-operative ulcers caused by ESD often lead to serious side effects.Post-ESD mu-cosal repair materials provide a new option for the treatment of post-ESD ulcers.In this study,we developed a polyurethane/small intestinal submucosa(PU/SIS)hydrogel and investigated its effi-cacy for accelerating ESD-induced ulcer healing in a canine model.PU/SIS hydrogel possessed great biocompatibility and distinctive pH-sensitive swelling properties and protected GES-1 cells from acid attack through forming a dense film in acidic conditions in vitro.Besides,PU/SIS gels present a strong bio-adhesion to gastric tissues under acidic conditions,thus ensuring the reten-tion time of PU/SIS gels in vivo.In a canine model,PU/SIS hydrogel was easily delivered via endoscopy and adhered to the ulcer sites.PU/SIS hydrogel accelerated gastric ulcer healing at an early stage with more epithelium regeneration and slight inflammation.Our findings reveal PU/SIS hydrogel is a promising and attractive candidate for ESD-induced ulcer repair.
文摘目的采用高通量测序技术检测溃疡性结肠炎(ulcerative colitis,UC)和正常人群的肠道菌群分布,挖掘与UC形成和发展显著相关的菌属结构,促进UC的研究和新药开发.方法提取各研究样本染色体DNA,并进行DNA片段长度检测和定量.对提取的DNA样本进行16S r RNA V3区基因扩增及焦磷酸测序.结果 U C患者的肠道菌群整体结构与健康对照人群具有显著的差异,主要表现为拟杆菌门、变形菌门和厚壁菌门丰度降低.其中在11个代谢通路中二组平均丰度存在显著差异(P<0.05).结论肠道菌群结构的异常改变与UC的发生发展存在较强相关性.多个代谢通路参与了UC的形成发展.通过分析疾病相关的关键菌群结构,有助于了解疾病的分子病理基础,并为新药设计提供可借鉴的理论依据.
