Partial amphiploids created by crossing common wheat （Triticum aestivum L.） and Thinopyrum ponticum （Podp.） Barkworth ＆ D. R. Dewey are important intermediates in wheat breeding because of their resistance to maj...Partial amphiploids created by crossing common wheat （Triticum aestivum L.） and Thinopyrum ponticum （Podp.） Barkworth ＆ D. R. Dewey are important intermediates in wheat breeding because of their resistance to major wheat diseases. In this study, we examined the chromosome compositions of five Xiaoyan-series wheat-Th, ponticum partial amphiploids （Xiaoyan 68, Xiaoyan 693, Xiaoyan 784, Xiaoyan 7430, and Xiaoyan 7631） using GISH, multicolor-GISH, and multicolor-FISH. We found several chromosome changes in these lines. For example, wheat chromosomes 1B and 2B were added in Xiaoyan 68 and Xiaoyan 7430, respectively, while wheat chromosome 6B was eliminated from Xiaoyan 693 and Xiaoyan 7631. Chromosome rearrangements were also detected in these amphiploids, including an interspecific translocation involving chromosome 4D and some intergenomic translocations, such as A--B and A--D translocations, among wheat genomes. Analysis of the Th. ponticum chromosomes in the amphiploids showed that some lines shared the same alien chromosomes. We also evaluated these partial amphiploids for resistance to nine races of stem rust, including TTKSK （commonly known as Ug99）. Three lines, Xiaoyan 68, Xiaoyan 784, and Xiaoyan 7430, exhibited excellent resistance to all nine races, and could therefore be valuable sources of stem rust resistance in wheat breeding.展开更多
The wild relatives of wheat （Triticum aestivum L.） contain tremendous amounts of potentially useful genes and represent a promising source of genetic diversity for wheat improvement （Bommineni and Jauhar, 1997）. T...The wild relatives of wheat （Triticum aestivum L.） contain tremendous amounts of potentially useful genes and represent a promising source of genetic diversity for wheat improvement （Bommineni and Jauhar, 1997）. Thinopyrum ponticum （Popd.） Barkworth and D. R. Dewey [syn. Agropyron elongatum （Host） P. Beauv., Elytrigia pontica （Podp.） Holub, Lophopy- rum ponticum （Podp.） A. L6ve] （2n = 10x = 70）, has high crossability with various Triticum species. Numerous studies have shown that Th. ponticum carries many potentially valu- able resistance genes against biotic and abiotic stresses （Shannon, 1978; Cox, 1991; Zheng et al., 2014a,b）. Transferring the useful genes from Th. ponticum to common wheat through chromosome engineering had been a successful way to enhance the resistance of wheat to pests and diseases （Sharma et al., 1989; McIntosh, 1991）.展开更多
Stem rust,caused by Puccinia graminis f.sp.tritici(Pgt),threatens global wheat production.Development of cultivars with increased resistance to stem rust by identification,mapping,and deployment of resistance genes is...Stem rust,caused by Puccinia graminis f.sp.tritici(Pgt),threatens global wheat production.Development of cultivars with increased resistance to stem rust by identification,mapping,and deployment of resistance genes is the best strategy for controlling the disease.In this study,we performed fine mapping and characterization of the all-stage stem rust resistance(Sr)gene Sr8155B1 from the durum wheat line 8155-B1.In seedling tests of biparental populations,Sr8155B1 was effective against six Chinese Pgt races tested.In a segregating population of 5060 gametes,Sr8155B1 was mapped to a 0.06-cM region flanked by markers Pku2772 and Pku43365,corresponding to 1.5-and 2.7-Mb regions in the Svevo and Chinese Spring reference genomes.Both regions include several typical nucleotide-binding leucine-rich repeat(NLR)and protein kinase genes that represent candidate genes.Among them,three NLR genes and three receptor-like protein kinases were highly polymorphic between the parental lines and their transcripts were upregulated in the homozygous resistant line TdR2 relative to its susceptible sister line TdS4.Four markers(Pku2772,Pku43365,Pku2950,and Pku3721)developed in this study,together with seedling resistance responses,correctly predicted Sr8155B1 absence or presence in 78 tetraploid wheat genotypes tested.The presence of Sr8155B1 in tetraploid wheat accessions CItr 14916,PI 197492,and PI 197493 was confirmed by mapping in three F_(2)populations.The genetic map and linked markers developed in this study may accelerate the deployment of Sr8155B1-mediated resistance in wheat breeding programs.展开更多
Stem rust, caused by Puccinia graminis Pers. f. sp. tritici Eriks ＆ E. Henn., is an important disease of wheat （Triticum aestivum L.） in many countries. To estimate grain yield losses due race Ug99 （TTKSK）, repli...Stem rust, caused by Puccinia graminis Pers. f. sp. tritici Eriks ＆ E. Henn., is an important disease of wheat （Triticum aestivum L.） in many countries. To estimate grain yield losses due race Ug99 （TTKSK）, replicated experiments including fifteen wheat cultivars were screened for seedling and adult plant resistance. Greenhouse seedling test was conducted under controlled conditions. Field experiments were set up at two locations, Mau-Narok and KARI-Njoro, during 2008 and 2009 cropping seasons. The stem rust epidemics were created by inoculating spreader rows of a susceptible variety planted perpendicular to rows of non-protected plots. Rust severity and infection response were determined using modified Cobb scale. All the cultivars were susceptible at seedling stage, but some displayed adult plant resistance response despite of the high disease pressure. Mean grain yield losses ranged between 5.6% and 66.3% and were highly correlated to final disease severity （FDS） and the area under disease progress curve （AUDPC）. Genetic studies are needed to characterise the adult plant resistance in tolerant culti（,ars to race Ug99 for exploitation in breeding for resistance.展开更多
Stem rust race Ug99, also designated TTKSK （Puccinia graminis f. sp. tritici） cause stem or black rust, which is a serious disease of wheat worldwide. Field experiments were conducted at two sites during 2008 and 20...Stem rust race Ug99, also designated TTKSK （Puccinia graminis f. sp. tritici） cause stem or black rust, which is a serious disease of wheat worldwide. Field experiments were conducted at two sites during 2008 and 2009 growing seasons to evaluate the effectiveness of two new foliar fungicides： viz. Nativo 300 SC （trifloxystrobin 100 g L^-1 ＋ tebuconazole 200 g L^-1） and Prosaro 250 EC （prothioconazole 125 g L^-1 ＋ tebuconazole 125 g L^-1）, in controlling stem rust on susceptible wheat cultivar Duma. AmistarXtra 280 SC （azoxystrobin 200 g L^-1 ＋ cyproconazole 80 g L^-1） and Folicur 250 EC （tebuconazole） were used as checks. The treatment at each site and year included non-treated control and two spray applications of the fungicides at growth stages （GS） 55 （heading） and 65 （flowering）. Stem rust severities were scored using the modified Cobb scale at 14-day intervals after application. The data were used to calculate mean rust severity （MRS）. Stem rust epidemics were severe at KARI-Njoro in 2008 and the treatment effects on stem rust severities, grain yield and 1,000 kernel weights were significant at both KARI-Njoro and Mau-Narok sites. The fungicide treatments, significantly （P _〈 0.05） reduced stem rust severity, increased grain yield and 1,000 kernel weight of the susceptible wheat cultivar Duma compared to the non-treated control. Both fungicides： Nativo 300 SC and Prosaro 250 EC applied at the rate of 1.0 L hat were recommended for commercial use.展开更多
Detection in 1999 of a new stem rust （Puccinia graminis f. sp. tritici） race Ug99 in Uganda with broad virulence including the virulence for Sr31 and its migration to Kenya and Ethiopia has been recognized as a sign...Detection in 1999 of a new stem rust （Puccinia graminis f. sp. tritici） race Ug99 in Uganda with broad virulence including the virulence for Sr31 and its migration to Kenya and Ethiopia has been recognized as a significant threat to local and world wheat production. All the Current Kenyan commercial varieties are susceptible to this race. This study was aimed at identifying suitable wheat varieties with resistance to Ug99 and replacing the susceptible commercial varieties through multi-locational testing and variety release. Thirty three lines were identified from a prescreen population of 104 lines and tested in 3 wheat growing regions in Kenya for two seasons in 2006 and 2007. The resulting four superior lines were evaluated under the National Performance Trial （NPT） where two lines which out-performed the best check variety were released as for commercial production. ＇Robin＇ was the best line and out yielded the commercial variety by 27%. ＂Eaglel0＂ was the second best and was better significantly than the check variety. These two lines which combined both adult plant resistant gene Sr2 complex and other major genes are expected to have some durable resistance and may be used to replace the current susceptible commercial varieties grown in Kenya.展开更多
InKenya, Russian wheat aphid (RWA) and stem rust race TTKS (“Ug99”) are the most devastating pests of wheat. Severe infestations by RWA result in yield losses of up to 90% while epidemics of “Ug99”can cause up to ...InKenya, Russian wheat aphid (RWA) and stem rust race TTKS (“Ug99”) are the most devastating pests of wheat. Severe infestations by RWA result in yield losses of up to 90% while epidemics of “Ug99”can cause up to 100% loss. The two pests combined have seriously affected farmer incomes forcing them to rely heavily on pesticides and increasing the cost of production. This study sought to evaluate a wheat line that has been developed to be resistant to both RWA and “Ug99”by pyramiding two major resistance genes. Three varieties were used in this study: “Kwale”, a Kenyan high yielding commercial variety but susceptible to both RWA and “Ug99”;“Cook”, an Australian variety carrying stem rust resistance gene Sr36 conferring immunity to “Ug99”;and “KRWA9”, a Kenyan line resistant to RWA but with poor agronomic attributes. The F1 of the double cross (DC F1) was obtained by crossing the F1 of “Kwale × Cook” and the F1 of “Kwale × KRWA9”. The DC F1 population was subjected to sequential screening for both RWA and “Ug99”resistance. The surviving DC F1 progenies were left to self pollinate in the field to obtain the DC F2. The DC F2 progenies were sequentially screened against RWA and “Ug99”to obtain a resistant population to both RWA and “Ug99”. The yield and yield components of the new resistant line were compared with the three parents. Results showed that the DC F2:3 had higher yields than the three parents based on 1000 kernel weight, weight of kernel per spike, and the actual yield in tons/ha, indicating that the genes were successfully introgressed. It is concluded that though races with virulence for Sr36 have been reported, the gene provides immunity to race “Ug99”and can be used as a component for “Ug99”resistance breeding together with other Sr genes.展开更多
Wheat is the second most important cereal in Kenya. However, production is severely constrained by both abiotic and biotic stresses. Of the biotic stresses a devastating pest (Russian wheat aphid (RWA)) and a serious ...Wheat is the second most important cereal in Kenya. However, production is severely constrained by both abiotic and biotic stresses. Of the biotic stresses a devastating pest (Russian wheat aphid (RWA)) and a serious disease (stem rust race TTKS (“Ug99”)) are currently the biggest problem for wheat producers in Kenya. Severe infestations by RWA may result in yield losses of up to 90% while “Ug99” infected fields may suffer 100% crop loss. The two pests combined are seriously affecting wheat farmers’ incomes because of the heavy reliance on pesticides that increase the cost of production. This study attempted to develop and characterize wheat lines that are resistant to both RWA and “Ug99” by pyramiding two major resistance genes. Three wheat varieties: “Kwale”, a Kenyan high yielding variety but susceptible to both RWA and “Ug99”;“Cook”, an Australian variety carrying stem rust resistance gene Sr36 conferring immunity to “Ug99”;and “KRWA9”, a Kenyan line with resistance to RWA but of poor agronomic attributes were used. A double cross F1 (DC F1) was obtained by crossing the F1 of “Kwale × Cook” and the F1 of “Kwale × KRWA9”. The DC F1 population was subjected to sequential screening for both RWA and “Ug99” resistance. Surviving DC F1 progenies were left to self pollinate to obtain the F2 of the double cross (DC F2). The DC F2 progenies were sequentially screened against RWA and “Ug99” to yield a population that was resistant to both RWA and “Ug99”. Genotyping of the DC F2:3 families were conducted to select homozygous resistant plants. Data indicated that the RWA and “Ug99” resistance genes were successfully pyramided. Though races with virulence for Sr36 have been reported, the gene provides immunity to race “Ug99” and can still be effectively used as a component for “Ug99” resistance breeding together with other Sr genes.展开更多
Dear Editor,Wheat stem rust is a devastating foliar disease caused by the fungal pathogen Puccinia graminis f.sp.tritici(Pgt).The emergence of new and highly virulent races of this pathogen(Lewis et al.,2018;Terefe et...Dear Editor,Wheat stem rust is a devastating foliar disease caused by the fungal pathogen Puccinia graminis f.sp.tritici(Pgt).The emergence of new and highly virulent races of this pathogen(Lewis et al.,2018;Terefe et al.,2024),including the Ug99 race group,has prompted global efforts to identify effective resistance genes.展开更多
Wheat along with rice and maize is fulfilling half of the calories demands of the world. Global Wheat production has increased tremendously since green revolution in 1960’s and helped in minimizing hunger and malnutr...Wheat along with rice and maize is fulfilling half of the calories demands of the world. Global Wheat production has increased tremendously since green revolution in 1960’s and helped in minimizing hunger and malnutrition. Developing countries, which consume 60% of the global wheat production, have shown a higher yield increase than the developed countries in the past [1]. It was driven by the hunger prevalence in these countries and was attributable to the introduction of high yielding and rusted resistant semi dwarf varieties developed under the collaborative efforts of International and National research systems during the last 50 years. Whereas, climate change and the emergence of new pests and diseases are threatening the food sustainability. The evolution of new races of disease pathogens like stem rust (Ug 99) is of serious concern. In order to feed the ever increasing population we have to increase wheat production at the rate 1.6% which can be achieved by developing high yielding varieties having a good tolerance level for biotic and abiotic stresses.展开更多
Wheat rusts,including stripe,leaf,and stem rusts,are severe wheat diseases and cause huge yield loss in China annually.Benefiting from utilizing the genetic resistance wheat varieties,wheat stem rust has been effectiv...Wheat rusts,including stripe,leaf,and stem rusts,are severe wheat diseases and cause huge yield loss in China annually.Benefiting from utilizing the genetic resistance wheat varieties,wheat stem rust has been effectively controlled since the 1970s;however,the wheat stripe and leaf rusts are still threating the wheat production in China due to lack of effective agricultural regulations.This review summarizes the research advances on wheat rust physiology,epidemiology,and fungicide resistance in China.In addition,the corresponding field management strategies for the integrated control of rust diseases are also discussed.展开更多
基金supported by the grants from the National Natural Science Foundation of China(No.31171539)the National High-Tech Research and Development Program of China(No.2011AA1001)the National Key Technology R&D Program of China(No.2013BAD05B01)
文摘Partial amphiploids created by crossing common wheat （Triticum aestivum L.） and Thinopyrum ponticum （Podp.） Barkworth ＆ D. R. Dewey are important intermediates in wheat breeding because of their resistance to major wheat diseases. In this study, we examined the chromosome compositions of five Xiaoyan-series wheat-Th, ponticum partial amphiploids （Xiaoyan 68, Xiaoyan 693, Xiaoyan 784, Xiaoyan 7430, and Xiaoyan 7631） using GISH, multicolor-GISH, and multicolor-FISH. We found several chromosome changes in these lines. For example, wheat chromosomes 1B and 2B were added in Xiaoyan 68 and Xiaoyan 7430, respectively, while wheat chromosome 6B was eliminated from Xiaoyan 693 and Xiaoyan 7631. Chromosome rearrangements were also detected in these amphiploids, including an interspecific translocation involving chromosome 4D and some intergenomic translocations, such as A--B and A--D translocations, among wheat genomes. Analysis of the Th. ponticum chromosomes in the amphiploids showed that some lines shared the same alien chromosomes. We also evaluated these partial amphiploids for resistance to nine races of stem rust, including TTKSK （commonly known as Ug99）. Three lines, Xiaoyan 68, Xiaoyan 784, and Xiaoyan 7430, exhibited excellent resistance to all nine races, and could therefore be valuable sources of stem rust resistance in wheat breeding.
基金supported by the grants from the National High-Tech Research and Development Program of China (No. 2011AA1001)the National Key Technology R&D Program of China (No. 2013BAD05B01)the National Natural Science Foundation of China (No. 31171539)
文摘The wild relatives of wheat （Triticum aestivum L.） contain tremendous amounts of potentially useful genes and represent a promising source of genetic diversity for wheat improvement （Bommineni and Jauhar, 1997）. Thinopyrum ponticum （Popd.） Barkworth and D. R. Dewey [syn. Agropyron elongatum （Host） P. Beauv., Elytrigia pontica （Podp.） Holub, Lophopy- rum ponticum （Podp.） A. L6ve] （2n = 10x = 70）, has high crossability with various Triticum species. Numerous studies have shown that Th. ponticum carries many potentially valu- able resistance genes against biotic and abiotic stresses （Shannon, 1978; Cox, 1991; Zheng et al., 2014a,b）. Transferring the useful genes from Th. ponticum to common wheat through chromosome engineering had been a successful way to enhance the resistance of wheat to pests and diseases （Sharma et al., 1989; McIntosh, 1991）.
基金the National Key Research and Development Program of China(2022YFD1201300)the Key R&D Program of Shandong Province(ZR202211070163)+1 种基金the Provincial Natural Science Foundation of Shandong(ZR2021ZD30,ZR2021MC056)the Young Taishan Scholars Program of Shandong Province.
文摘Stem rust,caused by Puccinia graminis f.sp.tritici(Pgt),threatens global wheat production.Development of cultivars with increased resistance to stem rust by identification,mapping,and deployment of resistance genes is the best strategy for controlling the disease.In this study,we performed fine mapping and characterization of the all-stage stem rust resistance(Sr)gene Sr8155B1 from the durum wheat line 8155-B1.In seedling tests of biparental populations,Sr8155B1 was effective against six Chinese Pgt races tested.In a segregating population of 5060 gametes,Sr8155B1 was mapped to a 0.06-cM region flanked by markers Pku2772 and Pku43365,corresponding to 1.5-and 2.7-Mb regions in the Svevo and Chinese Spring reference genomes.Both regions include several typical nucleotide-binding leucine-rich repeat(NLR)and protein kinase genes that represent candidate genes.Among them,three NLR genes and three receptor-like protein kinases were highly polymorphic between the parental lines and their transcripts were upregulated in the homozygous resistant line TdR2 relative to its susceptible sister line TdS4.Four markers(Pku2772,Pku43365,Pku2950,and Pku3721)developed in this study,together with seedling resistance responses,correctly predicted Sr8155B1 absence or presence in 78 tetraploid wheat genotypes tested.The presence of Sr8155B1 in tetraploid wheat accessions CItr 14916,PI 197492,and PI 197493 was confirmed by mapping in three F_(2)populations.The genetic map and linked markers developed in this study may accelerate the deployment of Sr8155B1-mediated resistance in wheat breeding programs.
文摘Stem rust, caused by Puccinia graminis Pers. f. sp. tritici Eriks ＆ E. Henn., is an important disease of wheat （Triticum aestivum L.） in many countries. To estimate grain yield losses due race Ug99 （TTKSK）, replicated experiments including fifteen wheat cultivars were screened for seedling and adult plant resistance. Greenhouse seedling test was conducted under controlled conditions. Field experiments were set up at two locations, Mau-Narok and KARI-Njoro, during 2008 and 2009 cropping seasons. The stem rust epidemics were created by inoculating spreader rows of a susceptible variety planted perpendicular to rows of non-protected plots. Rust severity and infection response were determined using modified Cobb scale. All the cultivars were susceptible at seedling stage, but some displayed adult plant resistance response despite of the high disease pressure. Mean grain yield losses ranged between 5.6% and 66.3% and were highly correlated to final disease severity （FDS） and the area under disease progress curve （AUDPC）. Genetic studies are needed to characterise the adult plant resistance in tolerant culti（,ars to race Ug99 for exploitation in breeding for resistance.
文摘Stem rust race Ug99, also designated TTKSK （Puccinia graminis f. sp. tritici） cause stem or black rust, which is a serious disease of wheat worldwide. Field experiments were conducted at two sites during 2008 and 2009 growing seasons to evaluate the effectiveness of two new foliar fungicides： viz. Nativo 300 SC （trifloxystrobin 100 g L^-1 ＋ tebuconazole 200 g L^-1） and Prosaro 250 EC （prothioconazole 125 g L^-1 ＋ tebuconazole 125 g L^-1）, in controlling stem rust on susceptible wheat cultivar Duma. AmistarXtra 280 SC （azoxystrobin 200 g L^-1 ＋ cyproconazole 80 g L^-1） and Folicur 250 EC （tebuconazole） were used as checks. The treatment at each site and year included non-treated control and two spray applications of the fungicides at growth stages （GS） 55 （heading） and 65 （flowering）. Stem rust severities were scored using the modified Cobb scale at 14-day intervals after application. The data were used to calculate mean rust severity （MRS）. Stem rust epidemics were severe at KARI-Njoro in 2008 and the treatment effects on stem rust severities, grain yield and 1,000 kernel weights were significant at both KARI-Njoro and Mau-Narok sites. The fungicide treatments, significantly （P _〈 0.05） reduced stem rust severity, increased grain yield and 1,000 kernel weight of the susceptible wheat cultivar Duma compared to the non-treated control. Both fungicides： Nativo 300 SC and Prosaro 250 EC applied at the rate of 1.0 L hat were recommended for commercial use.
文摘Detection in 1999 of a new stem rust （Puccinia graminis f. sp. tritici） race Ug99 in Uganda with broad virulence including the virulence for Sr31 and its migration to Kenya and Ethiopia has been recognized as a significant threat to local and world wheat production. All the Current Kenyan commercial varieties are susceptible to this race. This study was aimed at identifying suitable wheat varieties with resistance to Ug99 and replacing the susceptible commercial varieties through multi-locational testing and variety release. Thirty three lines were identified from a prescreen population of 104 lines and tested in 3 wheat growing regions in Kenya for two seasons in 2006 and 2007. The resulting four superior lines were evaluated under the National Performance Trial （NPT） where two lines which out-performed the best check variety were released as for commercial production. ＇Robin＇ was the best line and out yielded the commercial variety by 27%. ＂Eaglel0＂ was the second best and was better significantly than the check variety. These two lines which combined both adult plant resistant gene Sr2 complex and other major genes are expected to have some durable resistance and may be used to replace the current susceptible commercial varieties grown in Kenya.
文摘InKenya, Russian wheat aphid (RWA) and stem rust race TTKS (“Ug99”) are the most devastating pests of wheat. Severe infestations by RWA result in yield losses of up to 90% while epidemics of “Ug99”can cause up to 100% loss. The two pests combined have seriously affected farmer incomes forcing them to rely heavily on pesticides and increasing the cost of production. This study sought to evaluate a wheat line that has been developed to be resistant to both RWA and “Ug99”by pyramiding two major resistance genes. Three varieties were used in this study: “Kwale”, a Kenyan high yielding commercial variety but susceptible to both RWA and “Ug99”;“Cook”, an Australian variety carrying stem rust resistance gene Sr36 conferring immunity to “Ug99”;and “KRWA9”, a Kenyan line resistant to RWA but with poor agronomic attributes. The F1 of the double cross (DC F1) was obtained by crossing the F1 of “Kwale × Cook” and the F1 of “Kwale × KRWA9”. The DC F1 population was subjected to sequential screening for both RWA and “Ug99”resistance. The surviving DC F1 progenies were left to self pollinate in the field to obtain the DC F2. The DC F2 progenies were sequentially screened against RWA and “Ug99”to obtain a resistant population to both RWA and “Ug99”. The yield and yield components of the new resistant line were compared with the three parents. Results showed that the DC F2:3 had higher yields than the three parents based on 1000 kernel weight, weight of kernel per spike, and the actual yield in tons/ha, indicating that the genes were successfully introgressed. It is concluded that though races with virulence for Sr36 have been reported, the gene provides immunity to race “Ug99”and can be used as a component for “Ug99”resistance breeding together with other Sr genes.
文摘Wheat is the second most important cereal in Kenya. However, production is severely constrained by both abiotic and biotic stresses. Of the biotic stresses a devastating pest (Russian wheat aphid (RWA)) and a serious disease (stem rust race TTKS (“Ug99”)) are currently the biggest problem for wheat producers in Kenya. Severe infestations by RWA may result in yield losses of up to 90% while “Ug99” infected fields may suffer 100% crop loss. The two pests combined are seriously affecting wheat farmers’ incomes because of the heavy reliance on pesticides that increase the cost of production. This study attempted to develop and characterize wheat lines that are resistant to both RWA and “Ug99” by pyramiding two major resistance genes. Three wheat varieties: “Kwale”, a Kenyan high yielding variety but susceptible to both RWA and “Ug99”;“Cook”, an Australian variety carrying stem rust resistance gene Sr36 conferring immunity to “Ug99”;and “KRWA9”, a Kenyan line with resistance to RWA but of poor agronomic attributes were used. A double cross F1 (DC F1) was obtained by crossing the F1 of “Kwale × Cook” and the F1 of “Kwale × KRWA9”. The DC F1 population was subjected to sequential screening for both RWA and “Ug99” resistance. Surviving DC F1 progenies were left to self pollinate to obtain the F2 of the double cross (DC F2). The DC F2 progenies were sequentially screened against RWA and “Ug99” to yield a population that was resistant to both RWA and “Ug99”. Genotyping of the DC F2:3 families were conducted to select homozygous resistant plants. Data indicated that the RWA and “Ug99” resistance genes were successfully pyramided. Though races with virulence for Sr36 have been reported, the gene provides immunity to race “Ug99” and can still be effectively used as a component for “Ug99” resistance breeding together with other Sr genes.
基金supported by the Key R&D Program of Shandong Province,China(2024LZGC034 and 2023LZGC022)the Shandong Provincial Natural Science Foundation(SYS202206)+2 种基金the National Key Research and Development Program of China(2022YFD1201300)the National Natural Science Foundation of China(32472159)the Taishan Scholars Program.
文摘Dear Editor,Wheat stem rust is a devastating foliar disease caused by the fungal pathogen Puccinia graminis f.sp.tritici(Pgt).The emergence of new and highly virulent races of this pathogen(Lewis et al.,2018;Terefe et al.,2024),including the Ug99 race group,has prompted global efforts to identify effective resistance genes.
文摘Wheat along with rice and maize is fulfilling half of the calories demands of the world. Global Wheat production has increased tremendously since green revolution in 1960’s and helped in minimizing hunger and malnutrition. Developing countries, which consume 60% of the global wheat production, have shown a higher yield increase than the developed countries in the past [1]. It was driven by the hunger prevalence in these countries and was attributable to the introduction of high yielding and rusted resistant semi dwarf varieties developed under the collaborative efforts of International and National research systems during the last 50 years. Whereas, climate change and the emergence of new pests and diseases are threatening the food sustainability. The evolution of new races of disease pathogens like stem rust (Ug 99) is of serious concern. In order to feed the ever increasing population we have to increase wheat production at the rate 1.6% which can be achieved by developing high yielding varieties having a good tolerance level for biotic and abiotic stresses.
基金supported by the National Key Research and Development Program of China(2021YFD1401000)the National Natural Science Foundation of China(32072358,31871918,32272507,and 31071641)+1 种基金the Earmarked Fund for CARS-03,the Natural Science Basic Research Plan in Shaanxi Province of China(2020JZ-15)National‘111 Plan’(BP0719026).
文摘Wheat rusts,including stripe,leaf,and stem rusts,are severe wheat diseases and cause huge yield loss in China annually.Benefiting from utilizing the genetic resistance wheat varieties,wheat stem rust has been effectively controlled since the 1970s;however,the wheat stripe and leaf rusts are still threating the wheat production in China due to lack of effective agricultural regulations.This review summarizes the research advances on wheat rust physiology,epidemiology,and fungicide resistance in China.In addition,the corresponding field management strategies for the integrated control of rust diseases are also discussed.
