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改进的6轮Square算法中间相遇攻击
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作者 何峰 董晓丽 韦永壮 《计算机技术与发展》 2025年第2期63-69,共7页
Square分组密码算法是由Daemen等人设计,并在1997年快速软件加密(FSE)国际会议上首次公布。该密码算法基于SPN(Substitution-Permutation Network)结构,其分组长度和密钥长度均为128比特;具有设计新颖、实现高效等优点,也是高级加密标准... Square分组密码算法是由Daemen等人设计,并在1997年快速软件加密(FSE)国际会议上首次公布。该密码算法基于SPN(Substitution-Permutation Network)结构,其分组长度和密钥长度均为128比特;具有设计新颖、实现高效等优点,也是高级加密标准(AES)算法的前身,在密码学界备受关注。中间相遇攻击作为分组密码算法的重要分析方法之一,如何研究Square算法抵抗中间相遇攻击一直是业内讨论的热点问题。该文基于Square算法的结构特点和截断差分特征,利用差分枚举技术,构造了一个3.5轮中间相遇区分器。通过密钥桥技术及Square算法的密钥编排特点,推演出了主密钥与子密钥之间的部分线性关系。由此,将3.5轮区分器向前扩展1轮,向后扩展1.5轮,实现了对6轮Square算法的中间相遇攻击。该攻击所需数据复杂度为2105个选择明文,时间复杂度为2105次6轮加密,存储复杂度为285个分组。与已有攻击结果相比,新的攻击有效地降低了所需的数据复杂度、时间复杂度和存储复杂度。 展开更多
关键词 分组密码 square算法 中间相遇攻击 差分枚举技术 密钥桥技术
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Mechanical properties of wood materials using near-infrared spectroscopy based on correlation local embedding and partial least-squares 被引量:5
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作者 Lei Yu Yuliang Liang +1 位作者 Yizhuo Zhang Jun Cao 《Journal of Forestry Research》 SCIE CAS CSCD 2020年第3期1053-1060,共8页
This study used near-infrared(NIR)spectroscopy to predict mechanical properties of wood.NIR spectra were collected in wavelengths 900–1700 nm,and spectra averaged by radial and tangential surface spectra were used to... This study used near-infrared(NIR)spectroscopy to predict mechanical properties of wood.NIR spectra were collected in wavelengths 900–1700 nm,and spectra averaged by radial and tangential surface spectra were used to establish a partial least square(PLS)model based on correlation local embedding(CLE).Mongolian oak(Quercus mongolica Fisch.ex Ledeb.)was used to test the eff ectiveness of the model.The cross-validation method was used to verify the robustness of the CLE–PLS model.Ninety samples were tested as the calibration set and forty-fi ve as the validation set.The results show that the prediction coeffi cient of determination(R2 p)is 0.80 for MOR,and 0.78 for MOE.The ratio of performance to deviation is 2.23 for MOR and 2.15 for MOE. 展开更多
关键词 MODULUS of RUPTURE MODULUS of ELASTICITY Near-infrared CORRELATION LOCAL EMBEDDING Partial least square
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A novel noise reduction technique for underwater acoustic signals based on complete ensemble empirical mode decomposition with adaptive noise,minimum mean square variance criterion and least mean square adaptive filter 被引量:9
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作者 Yu-xing Li Long Wang 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2020年第3期543-554,共12页
Underwater acoustic signal processing is one of the research hotspots in underwater acoustics.Noise reduction of underwater acoustic signals is the key to underwater acoustic signal processing.Owing to the complexity ... Underwater acoustic signal processing is one of the research hotspots in underwater acoustics.Noise reduction of underwater acoustic signals is the key to underwater acoustic signal processing.Owing to the complexity of marine environment and the particularity of underwater acoustic channel,noise reduction of underwater acoustic signals has always been a difficult challenge in the field of underwater acoustic signal processing.In order to solve the dilemma,we proposed a novel noise reduction technique for underwater acoustic signals based on complete ensemble empirical mode decomposition with adaptive noise(CEEMDAN),minimum mean square variance criterion(MMSVC) and least mean square adaptive filter(LMSAF).This noise reduction technique,named CEEMDAN-MMSVC-LMSAF,has three main advantages:(i) as an improved algorithm of empirical mode decomposition(EMD) and ensemble EMD(EEMD),CEEMDAN can better suppress mode mixing,and can avoid selecting the number of decomposition in variational mode decomposition(VMD);(ii) MMSVC can identify noisy intrinsic mode function(IMF),and can avoid selecting thresholds of different permutation entropies;(iii) for noise reduction of noisy IMFs,LMSAF overcomes the selection of deco mposition number and basis function for wavelet noise reduction.Firstly,CEEMDAN decomposes the original signal into IMFs,which can be divided into noisy IMFs and real IMFs.Then,MMSVC and LMSAF are used to detect identify noisy IMFs and remove noise components from noisy IMFs.Finally,both denoised noisy IMFs and real IMFs are reconstructed and the final denoised signal is obtained.Compared with other noise reduction techniques,the validity of CEEMDAN-MMSVC-LMSAF can be proved by the analysis of simulation signals and real underwater acoustic signals,which has the better noise reduction effect and has practical application value.CEEMDAN-MMSVC-LMSAF also provides a reliable basis for the detection,feature extraction,classification and recognition of underwater acoustic signals. 展开更多
关键词 Underwater acoustic signal Noise reduction Empirical mode decomposition(EMD) Ensemble EMD(EEMD) Complete EEMD with adaptive noise(CEEMDAN) Minimum mean square variance criterion(MMSVC) Least mean square adaptive filter(LMSAF) Ship-radiated noise
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ASYMPTOTIC PROPERTY STUDY OF THE LEAST SQUARE ESTIMATES OF 2-D EXPONENTIAL SIGNALS VIA COMPLEX SIGNAL PROCESSING APPROACH 被引量:1
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作者 Mao Yongcai Bao Zheng (Key Laboratory for Radar Signal Processing, Xidian University, Xi’an 710071) 《Journal of Electronics(China)》 1999年第1期1-6,共6页
By use of the approach of complex random signal processing, the asymptotic statistical properties of the least square estimates of 2-D exponential signals are studied. In doing so it is found that the representation i... By use of the approach of complex random signal processing, the asymptotic statistical properties of the least square estimates of 2-D exponential signals are studied. In doing so it is found that the representation is considerably more intuitive, and is analytically more tractable. 展开更多
关键词 2-D complex EXPONENTIAL SIGNals Least squareS ESTIMATES ASYMPTOTIC STATISTICAL properties
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大豆GmALS2及其突变基因耐受苯磺隆的功能研究
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作者 郝飞宇 山琦 +5 位作者 杨明雨 王南 李晓薇 董园园 王法微 刘伟灿 《吉林农业大学学报》 北大核心 2025年第5期857-863,共7页
为挖掘大豆耐磺酰脲类除草剂(Sulfonylurea herbicides,SUs)的关键基因——聚焦乙酰乳酸合成酶(Acetolactate synthase,ALS)基因GmALS2,探究其第574位色氨酸突变为甘氨酸(W574G)对大豆耐苯磺隆(Tribenuron-methyl,TBM)能力的影响。通过... 为挖掘大豆耐磺酰脲类除草剂(Sulfonylurea herbicides,SUs)的关键基因——聚焦乙酰乳酸合成酶(Acetolactate synthase,ALS)基因GmALS2,探究其第574位色氨酸突变为甘氨酸(W574G)对大豆耐苯磺隆(Tribenuron-methyl,TBM)能力的影响。通过分析GmALS2基因在大豆不同组织及TBM胁迫下幼苗中的表达水平;克隆该基因,通过重叠PCR技术构建其W574G定点突变基因GmALS2(WG)。功能验证结果显示:GmALS2与GmALS2(WG)在酵母中表达后,均能提升酵母在含TBM培养基上的存活能力,且GmALS2(WG)的提升效果更显著;在大豆发状根中分别表达2个基因后,通过检测ALS酶底物丙酮酸的积累量,进一步证实GmALS2(WG)能更显著增强大豆发状根对TBM的耐受性。明确GmALS2基因W574G突变可增强大豆对TBM的耐受性,为筛选新的耐除草剂ALS突变位点及验证方案提供了参考。 展开更多
关键词 大豆 乙酰乳酸合成酶 als 除草剂 苯磺隆
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水稻抗甲咪唑烟酸突变体对多种ALS抑制剂类除草剂的抗性分析
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作者 王歆凯 李阳 +3 位作者 凌溪铁 裘实 张保龙 王金彦 《江苏农业学报》 北大核心 2025年第9期1665-1673,共9页
为创制抗乙酰乳酸合成酶(ALS)抑制剂类除草剂水稻种质、促进ALS抑制剂类除草剂在水稻生产中的应用,本研究以连粳15为试验材料,利用甲磺酸乙酯(Ethyl methanesulfonate,EMS)诱变和甲咪唑烟酸除草剂喷施处理,筛选获得抗甲咪唑烟酸的水稻... 为创制抗乙酰乳酸合成酶(ALS)抑制剂类除草剂水稻种质、促进ALS抑制剂类除草剂在水稻生产中的应用,本研究以连粳15为试验材料,利用甲磺酸乙酯(Ethyl methanesulfonate,EMS)诱变和甲咪唑烟酸除草剂喷施处理,筛选获得抗甲咪唑烟酸的水稻突变体,通过遗传分析和基因克隆明确突变体的突变位点及类型,并对野生型植株和突变体植株进行喷施无菌水(CK)和5种常用的ALS抑制剂类除草剂(甲氧咪草烟、甲咪唑烟酸、咪唑乙烟酸、甲基二磺隆和烟嘧磺隆)处理,分析突变体的抗性水平。结果表明,共筛选获得1个抗甲咪唑烟酸的水稻突变体,突变体ALS基因核苷酸序列在第1880位的碱基由G突变为碱基A,ALS蛋白氨基酸序列中第627个氨基酸由丝氨酸突变为天冬酰胺。喷施咪唑啉酮类除草剂甲氧咪草烟、甲咪唑烟酸和咪唑乙烟酸处理,野生型植株ALS活性、茎叶鲜重和根系鲜重、叶片叶绿素总含量均显著低于喷施无菌水对照(CK),突变体植株ALS活性、茎叶鲜重和根系鲜重、叶片叶绿素总含量与CK无显著差异。喷施磺酰脲类除草剂甲基二磺隆和烟嘧磺隆处理,野生型植株和突变体植株ALS活性、茎叶鲜重和根系鲜重、叶片叶绿素总含量均显著低于CK。因此,本研究获得的突变体对咪唑啉酮类除草剂具有较强的抗性,但对磺酰脲类除草剂抗性相对较差。 展开更多
关键词 als抑制剂类除草剂 水稻突变体 咪唑啉酮类除草剂 磺酰脲类除草剂 乙酰乳酸合成酶(als)
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Correlation analysis and partial least square modeling to quantify typical minerals with Chang'E-3 visible and near-infrared imaging spectrometer's ground validation data 被引量:3
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作者 LIU Bin LIU Jianzhong +5 位作者 ZHANG Guangliang LING Zongcheng ZHANG Jiang HE Zhiping YANG Benyong ZOU Yongliao 《Chinese Journal Of Geochemistry》 EI CAS CSCD 2014年第1期86-94,共9页
In 2013, Chang'E-3 program will develop lunar mineral resources in-situ detection. A Visible and Near-infrared Imaging Spectrometer(VNIS) has been selected as one payload of CE-3 lunar rover to achieve this goal. ... In 2013, Chang'E-3 program will develop lunar mineral resources in-situ detection. A Visible and Near-infrared Imaging Spectrometer(VNIS) has been selected as one payload of CE-3 lunar rover to achieve this goal. It is critical and urgent to evaluate VNIS' spectrum data quality and validate quantification methods for mineral composition before its launch. Ground validation experiment of VNIS was carried out to complete the two goals, by simulating CE-3 lunar rover's detection environment on lunar surface in the laboratory. Based on the hyperspectral reflectance data derived, Correlation Analysis and Partial Least Square(CA-PLS) algorithm is applied to predict abundance of four lunar typical minerals(pyroxene, plagioclase, ilmenite and olivine) in their mixture. We firstly selected a set of VNIS' spectral parameters which highly correlated with minerals' abundance by correlation analysis(CA), and then stepwise regression method was used to find out spectral parameters which make the largest contributions to the mineral contents. At last, functions were derived to link minerals' abundance and spectral parameters by partial least square(PLS) algorithm. Not considering the effect of maturity, agglutinate and Fe0, we found that there are wonderful correlations between these four minerals and VNIS' spectral parameters, e.g. the abundance of pyroxene correlates positively with the mixture's absorption depth, the value of absorption depth added as the increasing of pyroxene's abundance. But the abundance of plagioclase correlates negatively with the spectral parameters of band ratio, the value of band ratio would decrease when the abundance of plagioclase increased. Similar to plagioclase, the abundance of ilmenite and olivine has a negative correlation with the mixture's reflectance data, if the abundance of ilmenite or olivine increase, the reflectance values of the mixture will decrease. Through model validation, better estimates of pyroxene, plagioclase and ilmenite's abundances are given. It is concluded that VNIS has the capability to be applied on lunar minerals' identification, and CA-PLS algorithm has the potential to be used on lunar surface's in-situ detection for minerals' abundance prediction. 展开更多
关键词 红外成像光谱仪 偏最小二乘 矿物成分 地面验证 相关分析 模型验证 可见光 高光谱反射率
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Summation of Reciprocals Related to Square of Products of Fibonacci Numbers
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作者 席高文 王淑玉 《Chinese Quarterly Journal of Mathematics》 CSCD 2009年第3期400-406,共7页
By applying the method of on summation by parts,the purpose of this paper is to give several reciprocal summations related to squares of products of the Fibonacci numbers.
关键词 Fibonacci numbers SUMMATION square reciprocal
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热处理对选区激光熔化AlSi10Mg合金组织与性能的影响 被引量:1
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作者 李智勇 张珞 +4 位作者 陈锐敏 王俊 刘奋成 尤启凡 胡晓安 《热加工工艺》 北大核心 2025年第13期31-35,共5页
AlSi10Mg铝合金具有高比强度和高耐磨性等特点,被广泛应用于航空航天、汽车及船舶制造等领域。利用选区激光熔化技术制备AlSi10Mg铝合金,探究不同热处理工艺对AlSi10Mg铝合金组织及力学性能的影响。结果表明,随着热处理温度的提高,共晶S... AlSi10Mg铝合金具有高比强度和高耐磨性等特点,被广泛应用于航空航天、汽车及船舶制造等领域。利用选区激光熔化技术制备AlSi10Mg铝合金,探究不同热处理工艺对AlSi10Mg铝合金组织及力学性能的影响。结果表明,随着热处理温度的提高,共晶Si组织被破坏并且合金中颗粒状Si相的尺寸不断增加,合金的强度和硬度呈现下降趋势,但塑性提升明显。热处理改变了合金的断裂方式,沉积态下AlSi10Mg铝合金为脆性断裂,而热处理后则转变为韧性断裂。 展开更多
关键词 选区激光熔化 alsi10Mg铝合金 力学性能 热处理
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On a New Family of Weighted Least-square Orthogonal Polynomials in Multivariables
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作者 郑成德 王仁宏 《Northeastern Mathematical Journal》 CSCD 2003年第4期339-345,共7页
This paper introduces a new notion of weighted least-square orthogonal polynomials in multivariables from the triangular form. Their existence and uniqueness is studied and some methods for their recursive computation... This paper introduces a new notion of weighted least-square orthogonal polynomials in multivariables from the triangular form. Their existence and uniqueness is studied and some methods for their recursive computation are given. As an application, this paper constructs a new family of Pade-type approximates in multi-variables from the triangular form. 展开更多
关键词 orthogonal polynomials least square Pade-type approximate multi-variate approximation
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JAK2/p-JAK2在ALS-SOD1-G93A转基因小鼠发病及进展中的表达
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作者 齐银矿 文岚 +2 位作者 余建萍 李堃毅 刘勇 《中南医学科学杂志》 2025年第2期221-225,共5页
目的 观察酪氨酸激酶2(JAK2)/磷酸化JAK2(p-JAK2)在肌萎缩侧索硬化(ALS)-超氧化物歧化酶1(SOD1)-G93A转基因小鼠发病及进展中的作用。方法 构建带有人类SOD1突变基因的ALS-SOD1-G93A转基因小鼠。用运动缺陷评分系统评价小鼠运动功能,观... 目的 观察酪氨酸激酶2(JAK2)/磷酸化JAK2(p-JAK2)在肌萎缩侧索硬化(ALS)-超氧化物歧化酶1(SOD1)-G93A转基因小鼠发病及进展中的作用。方法 构建带有人类SOD1突变基因的ALS-SOD1-G93A转基因小鼠。用运动缺陷评分系统评价小鼠运动功能,观察小鼠行为学,然后随机取30、60、90天日龄、ALS发病期(onset)、终末期ALS-SOD1-G93A转基因小鼠及130天日龄非转基因小鼠(non-Tg)各6只,分别为30天组、60天组、90天组、onset组、终末期组及non-Tg组。取各组小鼠脊髓腰膨大部,利用Western blotting和免疫组织化学实验观察JAK2、p-JAK2蛋白表达水平。结果 JAK2在ALS-SOD1-G93A转基因小鼠各时期和non-Tg小鼠脊髓腰膨大部中阳性表达均较弱,其中,30天组、60天组、90天组可见具有细胞形态的弱阳性表达,onset组、终末期组表达略增多;non-Tg组、30天组、60天组、90天组p-JAK2阳性表达较弱,主要集中在脊髓前角灰质的运动神经元胞质,但30天组、60天组、90天组偶见呈p-JAK2强阳性表达的胶质细胞,并累及白质,随小鼠日龄增加呈p-JAK2强阳性表达的胶质细胞数量增多,onset组、终末期组p-JAK2阳性表达于胶质细胞且明显增强,广泛累及腰髓灰质和白质。Western blotting结果显示,终末期组JAK2、p-JAK2蛋白表达水平较non-Tg组、30天组、60天组明显增高(P<0.05)。结论 在ALS-SOD1-G93A转基因小鼠发病过程中,JAK2、p-JAK2表达显著上调,p-JAK2主要表达在发病后ALS-SOD1-G93A转基因小鼠增生的胶质细胞,提示JAK2/p-JAK2在ALS发病机制中具有重要作用。 展开更多
关键词 als JAK2 p-JAK2 SOD1 神经变性疾病 als-SOD1-G93A转基因小鼠
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基于认知负荷理论的ALS患者眼控交互界面优化研究
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作者 何钰彬 陈洋 《包装工程》 北大核心 2025年第18期117-126,158,共11页
目的降低ALS患者的认知负荷,提出一套适用于ALS患者的眼控交互界面设计原则。方法选用市面上ALS患者使用的眼控交互界面进行实验,实验主要分为自由浏览测试、任务测试、主观评价测试3个阶段。根据实验结果发掘界面存在的问题,并提出相... 目的降低ALS患者的认知负荷,提出一套适用于ALS患者的眼控交互界面设计原则。方法选用市面上ALS患者使用的眼控交互界面进行实验,实验主要分为自由浏览测试、任务测试、主观评价测试3个阶段。根据实验结果发掘界面存在的问题,并提出相应的设计原则,运用设计原则对原界面进行设计优化,得到优化后的界面。再根据眼动数据(眨眼率、眨眼时间、总注视点数、瞳孔直径、热区图、AOI注视率)、绩效数据(任务完成时间、任务完成率)和主观数据(NASA-TLX量表),对比分析原界面和优化后界面的认知负荷。结果优化后界面的眼动数据和绩效数据均优于原界面,主观认知负荷也小于原界面。结论提出的ALS患者使用的眼控交互界面设计原则具有一定可行性,主、客观认知负荷综合分析的方法适用于ALS患者的眼控交互界面优化分析。 展开更多
关键词 眼控交互 认知负荷 肌萎缩侧索硬化症(als) 界面设计 关怀体验
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ON A FAMILY OF MULTIVARIATE LEAST-SQUARES ORTHOGONAL POLYNOMIALS 被引量:1
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作者 郑成德 王仁宏 《Numerical Mathematics A Journal of Chinese Universities(English Series)》 SCIE 2003年第1期51-58,共8页
In this paper the new notion of multivariate least-squares orthogonal poly-nomials from the rectangular form is introduced. Their existence and uniqueness isstudied and some methods for their recursive computation are... In this paper the new notion of multivariate least-squares orthogonal poly-nomials from the rectangular form is introduced. Their existence and uniqueness isstudied and some methods for their recursive computation are given. As an applica-is constructed. 展开更多
关键词 线性代数 正交多项式 多元最小二乘方 递归计算 多元近似
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一种基于Least Square Method算法的城轨车辆车门动作时间精准判断的研究
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作者 李宏菱 宋华杰 +3 位作者 马仲智 周辉 李晴 陈龙 《时代汽车》 2025年第3期190-192,共3页
为研究城市轨道交通车辆客室车门动作时间精准性,门的动作主要依靠直流无刷电机的驱动,所以门动作判断的根本,是对电机运动状态的判读,门运动过程中由于电机码盘线受杂波干扰,系统无法准确寻找计时点从而影响系统判断门运动时间;建立波... 为研究城市轨道交通车辆客室车门动作时间精准性,门的动作主要依靠直流无刷电机的驱动,所以门动作判断的根本,是对电机运动状态的判读,门运动过程中由于电机码盘线受杂波干扰,系统无法准确寻找计时点从而影响系统判断门运动时间;建立波形矫正模型,利用数学方法校准波形,让MCU找出最佳计时点并处理(误差不超过10ms),采用最小二乘法模型,通过最小化误差的平方和找到一组数据的最佳函数匹配,求得未知的数据,并使得这些求得的数据与实际数据之间误差的平方和为最小,可精准地得到门动作时间。模拟测试结果表明,门动作时间测算误差所示其误差为7.42ms,小于10ms。 展开更多
关键词 城轨车辆 客室车门 电机码盘 Least square Method算法 门动作时间精准
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ALS2基因突变致婴儿起病型上行性遗传性痉挛性截瘫家系2例报道
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作者 朱子豪 华冉 +4 位作者 姜俊红 王宝田 吴德 唐久来 杨李 《中国优生与遗传杂志》 2025年第2期363-372,共10页
目的探讨ALS2基因突变导致婴儿起病型上行性遗传性痉挛性截瘫(IAHSP)临床表型及遗传学特点。方法回顾性分析ALS2基因突变致婴儿起病型上行性遗传性痉挛性截瘫先证者临床资料及家系特点,并应用全外显子组测序分析患儿的致病基因,再用San... 目的探讨ALS2基因突变导致婴儿起病型上行性遗传性痉挛性截瘫(IAHSP)临床表型及遗传学特点。方法回顾性分析ALS2基因突变致婴儿起病型上行性遗传性痉挛性截瘫先证者临床资料及家系特点,并应用全外显子组测序分析患儿的致病基因,再用Sanger测序进行家系验证,复习相关文献,总结其临床特点及遗传学特征。结果家系1先证者及其姐姐检测到ALS2基因突变位点为c.2687G>A的纯合突变,家系2先证者为c.4221delA和c.575C>T的复合杂合突变;主要表现为运动功能的倒退,下肢痉挛、肌力下降,失去独走能力,合并智力发育存在落后,但头颅影像学无特异性发现;检索到中国知网、万方数据库和PubMed数据库自建库至2023年12月的26篇文献(其中英文25篇,中文1篇),共报道IAHSP77例(包括本文3例)。结论ALS2基因突变致IAHSP,早期症状和痉挛型脑瘫相似,近亲婚配史是纯合突变发生的主要原因,ALS2基因突变多位于RCC1结构域,上肢受累是普遍现象,虽然大多智力正常,但球麻痹和是否可独坐及独立行走决定患儿生活质量,早期干预有重要意义;常规的头颅MRI影像学结果异常率不高,且缺乏特异性。 展开更多
关键词 als2基因 婴儿起病型上行性遗传性痉挛性截瘫 遗传学诊断
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大鼠中敲除SOD1导致肌萎缩侧索硬化症(ALS)表型
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作者 李新月 潘烁 +6 位作者 张磊 贺佳跃 刘宁 张旭 高珊 齐晓龙 马元武 《中国实验动物学报》 北大核心 2025年第11期1598-1609,共12页
目的 建立超氧化物歧化酶1(SOD1)基因敲除大鼠,解析其行为学表型。方法 采用CRISPR/Cas9技术构建SOD1敲除型大鼠,通过PCR及Sanger测序进行基因型鉴定,Western Blot验证SOD1蛋白表达情况。与野生型大鼠交配,评估基因型遗传稳定性。动态... 目的 建立超氧化物歧化酶1(SOD1)基因敲除大鼠,解析其行为学表型。方法 采用CRISPR/Cas9技术构建SOD1敲除型大鼠,通过PCR及Sanger测序进行基因型鉴定,Western Blot验证SOD1蛋白表达情况。与野生型大鼠交配,评估基因型遗传稳定性。动态监测体质量变化、生存率,系统评估组织病变、行为学异常、脊髓运动神经元数量变化及炎症变化情况。结果 基因型鉴定确认SOD1敲除型大鼠模型制备成功。SOD1基因缺失影响大鼠发育及体质量。行为学显示敲除型大鼠表现出渐进性的运动共济失调,最终导致后肢瘫痪,伴随有脊髓L4区运动神经元丧失,小胶质细胞显著增多、腓肠肌肌细胞数量与横截面积减少等肌萎缩侧索硬化症(ALS)疾病特征。结论 本研究成功构建SOD1敲除型大鼠模型,该模型能重现ALS疾病的一些核心病理特征,为深入研究SOD1基因在ALS中的功能提供了重要动物模型。 展开更多
关键词 SOD1 敲除型大鼠 肌萎缩侧索硬化症 als
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烤烟K326的NtALS基因单碱基编辑与抗除草剂材料创制
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作者 孙小琼 张孝廉 +9 位作者 郭玉双 胡建林 胡鹏 郑兴飞 王红波 薛莲 徐得泽 钟余平 殷得所 王丰 《湖北农业科学》 2025年第5期102-104,111,共4页
为培育抗除草剂的烤烟新品种,利用基因编辑技术对烤烟K326的NtALS基因进行单碱基编辑替换,将烟草NtALS基因的第194位脯氨酸三联体密码CCA替换成亮氨酸编码CTA和TTA,测序结果表明编辑效率达到了20.69%,阳性编辑植株的除草剂喷施抗性鉴定... 为培育抗除草剂的烤烟新品种,利用基因编辑技术对烤烟K326的NtALS基因进行单碱基编辑替换,将烟草NtALS基因的第194位脯氨酸三联体密码CCA替换成亮氨酸编码CTA和TTA,测序结果表明编辑效率达到了20.69%,阳性编辑植株的除草剂喷施抗性鉴定结果显示抗性明显,创制了抗除草剂的烤烟新材料。 展开更多
关键词 乙酰乳酸合成酶 烤烟K326 除草剂 基因编辑
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Laser-implanted short fiber-like interface structure for strengthening welde d-braze d joint of Al/steel dissimilar metals 被引量:1
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作者 Lize Li Jianyu Li +4 位作者 Shuhai Chen Shujun Chen Jian Yang Jihua Huang Gaoyang Yu 《Journal of Materials Science & Technology》 2025年第34期68-82,共15页
The lamellar layer of intermetallic compounds(IMCs)was adversely affected the performance of welding-brazing joints in Al/steel dissimilar metals.In this study,a short fiber-like surface morphology was fabricated on t... The lamellar layer of intermetallic compounds(IMCs)was adversely affected the performance of welding-brazing joints in Al/steel dissimilar metals.In this study,a short fiber-like surface morphology was fabricated on the butt surface of Q235 steel via laser.The interaction behavior between the short fibers and the molten pool was captured using a high-speed camera.Laser-arc hybrid welding-brazing was then employed to join Al(6061-T6)to the steel.This process successfully created a short fiber-like interface structure at the joint.The relationship between microstructure and mechanical properties was investigated,compared with Al/bare steel(ABS)joint.The research results indicated that the IMCs layer consisted of FeAl_(3)and Fe_(2)Al_(5).The interface strength of the Al/short fiber-like surface structural steel(ASFSSS)joint reached 153.2 MPa,an 82.2%increase compared to the ABS joint,which reached 84.1 MPa.When the ASFSSS joints without the reinforcement were bent to 58.2°and 25.2°in the longitudinal and transverse direction,respectively,they remained intact.However,cracks were discovered when the bending angle of the ABS reached 39.1°and 0°in the two directions.Numerical simulation revealed that the short fiber-like interface structure significantly reduced residual stress and improved the stress distribution in the weld,thereby enhancing the strength and toughness of Al/steel dissimilar joints.The crack propagation path in the ASFSSS joint was deflected into the weld when it encountered short fibers,and the fracture morphology presented the characteristic of ductile-brittle mixed fracture. 展开更多
关键词 Short fiber-like interface structure Laser-arc welding-brazing Al/steel dissimilar joint Intermetallic compounds Mechanical properties
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Design Process of a Memorial Square to Thank and Respect for Healthcare Workers after Covid-19 Pandemic
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作者 Ozgur Yerli 《Journal of Environmental & Earth Sciences》 2025年第2期1-12,共12页
Open and green spaces are essential elements of cities.With the COVID epidemic,the importance of the need for open and green spaces in urban areas has become more understood.The aim of this study is to create a new an... Open and green spaces are essential elements of cities.With the COVID epidemic,the importance of the need for open and green spaces in urban areas has become more understood.The aim of this study is to create a new and contemporary square in the working area where the current use and traffic create chaos,to add a new square to the open and green areas in Ankara(Turkey)and to create a connection between this area and the existing open and green areas.At the same time,health workers are honoured through a memorial area integrated into the square.The importance of the location chosen as the study area is that it is surrounded by many public health facilities and is a point of intersection of Ankara’s existing open green space systems.Throughout the three-stage design process,brainstorming sessions were held,drawings and sketches were made,and urban analyses were carried out on transport,culture,art,education,health,open and green spaces.Solutions were proposed for both indoor and outdoor spaces,and the forms were transformed into functional design elements and reflected in the space.As a result,after the pandemic,a city square has been created where people can socialise again,with a monument,a square,water surfaces,an amphitheatre,viewing terraces,a market area,a bridge,sitting and walking areas that are appropriate to the capital. 展开更多
关键词 Design MEMORIAL MONUMENT square Urban
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Achieved strength-ductility simultaneous enhancement of AZ31 magnesium alloy square tube through a novel tube continuous expanding shear extrusion processing
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作者 Wei Zhang Chaowei Zeng +6 位作者 Hui Zhao Hongjun Hu Dingfei Zhang Rui Hong Ting Yuan Zhenwei Sun Wei Peng 《Journal of Magnesium and Alloys》 2025年第5期2390-2415,共26页
Recently,there have been few reports on the hot extrusion process of magnesium(Mg)alloy square tubes.In this work,we introduce a novel hot extrusion process called tube continuous expanding shear extrusion(t CESE)to p... Recently,there have been few reports on the hot extrusion process of magnesium(Mg)alloy square tubes.In this work,we introduce a novel hot extrusion process called tube continuous expanding shear extrusion(t CESE)to produce Mg alloy square tubes with heterogeneous structures.Compared to previously reported hot-extruded Mg alloy tubes,the tCESE samples exhibit simultaneous enhancements in both strength and ductility.The microstructure evolution during the t CESE process was analyzed in detail.It was found that the microstructure developed consecutively as a result of twinning-induced dynamic recrystallization(TDRX),discontinuous dynamic recrystallization(DDRX)and continuous dynamic recrystallization(CDRX)with different nucleation of new orientations,while static recrystallization(SRX)promoted the formation of heterogeneous structures.The strengthening mechanism in the t CESE samples was revealed by loading-unloading-reloading(LUR)tensile tests,viscoplastic self-consistent(VPSC)simulation and intermittent tensile tests.The improvement of the strength in the tCESE samples is attributed to the size refinement of the grains and the second-phases,and more importantly,to hetero-deformation induced(HDI)strengthening.The improvement of the ductility in the t CESE samples can be attributed to the significant increase in non-basal dislocations within the heterogeneous structures during tensile deformation. 展开更多
关键词 Mg alloy square tube Heterogeneous structures Mechanical properties
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