摘要
为培育抗除草剂的烤烟新品种,利用基因编辑技术对烤烟K326的NtALS基因进行单碱基编辑替换,将烟草NtALS基因的第194位脯氨酸三联体密码CCA替换成亮氨酸编码CTA和TTA,测序结果表明编辑效率达到了20.69%,阳性编辑植株的除草剂喷施抗性鉴定结果显示抗性明显,创制了抗除草剂的烤烟新材料。
In order to breed new varieties of flue-cured tobacco resistant to herbicide,the NtALS gene of flue-cured tobacco K326 was edited by the single base,the 194th proline triad code CCA of tobacco NtALS gene was replaced with leucine encoding CTA and TTA.Sequencing results showed that the editing rate was up to 20.69%,the identification results of the herbicide spraying resistance of positive edited plants showed that the resistance was obvious,and a new flue-cured tobacco material with herbicide resistance was developed.
作者
孙小琼
张孝廉
郭玉双
胡建林
胡鹏
郑兴飞
王红波
薛莲
徐得泽
钟余平
殷得所
王丰
SUN Xiao-qiong;ZHANG Xiao-lian;GUO Yu-shuang;HU Jian-lin;HU Peng;ZHENG Xing-fei;WANG Hong-bo;XUE Lian;XU De-ze;ZHONG YU-ping;YIN De-suo;WANG Feng(Institute of Food Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;Hubei Huichuzhi Biotech Company,Wuhan 430064,China;Guizhou Academy of Tobacco Science,Guiyang 550081,China)
出处
《湖北农业科学》
2025年第5期102-104,111,共4页
Hubei Agricultural Sciences
基金
贵州省烟草公司科技项目(2022XM04)
武汉市科技计划项目(2022022202015037)。
