Epigenetic regulation is critical to multiple physiological and pathological processes.However,little is known regarding the epigenetic changes during neonatal skin development and skin aging,and in response to ultrav...Epigenetic regulation is critical to multiple physiological and pathological processes.However,little is known regarding the epigenetic changes during neonatal skin development and skin aging,and in response to ultraviolet(UV)exposure.The transcriptomes of human skin samples from different ages or irradiated with different types and doses of UV light were analyzed using R(version 4.0.3)software.The epigenetic landscape of the skin,including histone modifications,genetic imprinting and m^(6)A modification,which are mainly involved in collagen formation,extracellular matrix organization,immune function and keratinization,underwent significant changes during neonatal to adult development.Epigenetic effectors such as IGF2BP2,GATA2,GATA3,CPA4 and CDK1 were significantly correlated with extracellular matrix organization,and VEGFA,CDK1 and PRKCB with skin immune function.The m^(6)A readers such as IGF2BP2,IGF2BP3,HNRNPA2B1 and EIF3G showed significant correlation with extracellular matrix organization,metabolism,or antigen processing and presentation.Small doses of UV exposure only induced changes in the expression levels of some epigenetic effectors,without any significant effect on the overall epigenetic landscape.However,the minimal erythema dose of UV exposure altered multiple epigenetic effectors regulating extracellular matrix organization,cell-matrix adhesion,innate immune response,mitochondrial function and mRNA processing.In addition,epigenetic changes following UV exposure were more pronounced in the elderly skin compared to the younger skin.In conclusion,histone modifications,genetic imprinting and m^(6)A modification play critical roles during skin development,and a large dose of UV exposure can significantly change the expression of multiple epigenetic effectors.展开更多
目的探讨髋关节周围皮纹不对称与发育性髋关节发育不良(developmental dysplasia of the hip,DDH)的关系。方法回顾性分析2017年1月至2022年12月在徐州医科大学附属徐州儿童医院进行DDH筛查的308名婴儿。根据筛查原因将其分为观察组(单...目的探讨髋关节周围皮纹不对称与发育性髋关节发育不良(developmental dysplasia of the hip,DDH)的关系。方法回顾性分析2017年1月至2022年12月在徐州医科大学附属徐州儿童医院进行DDH筛查的308名婴儿。根据筛查原因将其分为观察组(单纯皮纹不对称,n=116例)、临床异常组(具有髋关节弹响、髋关节外展受限、双下肢不等长等临床表现,n=92)和对照组(单纯行髋关节筛查,n=100)。分析临床评估结果及Graf法髋关节超声检查结果,比较各组病理性DDH的检出率,计算髋关节周围皮纹不对称诊断病理性DDH的阳性预测值、阴性预测值、敏感度、特异性。结果观察组检出病理性DDH 5例(4.31%);临床异常组检出病理性DDH 28例(30.43%);对照组检出病理性DDH 4例(4.00%)。观察组和对照组婴儿的病理性DDH检出率比较差异无统计学意义(χ^(2)=0.909,P=0.592);临床异常组婴儿的病理性DDH检出率显著高于对照组(χ^(2)=24.109,P<0.001)。单纯髋关节周围皮纹不对称对病理性DDH的阳性预测值为4.31%,阴性预测值为96.00%,敏感度为55.56%,特异性为46.38%。出现异常临床表现对病理性DDH的阳性预测值为30.43%,阴性预测值为96.00%,敏感度为87.50%,特异性为60.00%。结论单纯髋关节周围皮纹不对称诊断病理性DDH的价值较低,应更加重视是否存在其他临床体征,以指导婴儿的影像学筛查。展开更多
Deep skin wounds heal by scar formation with a Joss of its original appearance, structure and function.However, when the same damage occurs to the skin of an early gestational fetus, complete regeneration can be obser...Deep skin wounds heal by scar formation with a Joss of its original appearance, structure and function.However, when the same damage occurs to the skin of an early gestational fetus, complete regeneration can be observed. Despite significant research in the field of skin regeneration, many mysteries remain, such as the loss of wound healing ability with maturity, the differences in healing at different parts of the body, and the presence of hypertrophic scars and keloids in some races but not in others. The finding of HOX genes in the skin provides new explanations to these conundrums.展开更多
基金supported by the National Natural Science Foundation of China(82073421 and 82003326)the Wisdom Accumulation and Talent Cultivation Project of the Third Xiangya Hospital of Central South University(YX202007)+1 种基金the science and technology innovation Program of Hunan Province(2021RC3035)Natural Science Foundation of Hunan Province(2021JJ40924 and 2021JJ20089).
文摘Epigenetic regulation is critical to multiple physiological and pathological processes.However,little is known regarding the epigenetic changes during neonatal skin development and skin aging,and in response to ultraviolet(UV)exposure.The transcriptomes of human skin samples from different ages or irradiated with different types and doses of UV light were analyzed using R(version 4.0.3)software.The epigenetic landscape of the skin,including histone modifications,genetic imprinting and m^(6)A modification,which are mainly involved in collagen formation,extracellular matrix organization,immune function and keratinization,underwent significant changes during neonatal to adult development.Epigenetic effectors such as IGF2BP2,GATA2,GATA3,CPA4 and CDK1 were significantly correlated with extracellular matrix organization,and VEGFA,CDK1 and PRKCB with skin immune function.The m^(6)A readers such as IGF2BP2,IGF2BP3,HNRNPA2B1 and EIF3G showed significant correlation with extracellular matrix organization,metabolism,or antigen processing and presentation.Small doses of UV exposure only induced changes in the expression levels of some epigenetic effectors,without any significant effect on the overall epigenetic landscape.However,the minimal erythema dose of UV exposure altered multiple epigenetic effectors regulating extracellular matrix organization,cell-matrix adhesion,innate immune response,mitochondrial function and mRNA processing.In addition,epigenetic changes following UV exposure were more pronounced in the elderly skin compared to the younger skin.In conclusion,histone modifications,genetic imprinting and m^(6)A modification play critical roles during skin development,and a large dose of UV exposure can significantly change the expression of multiple epigenetic effectors.
文摘目的探讨髋关节周围皮纹不对称与发育性髋关节发育不良(developmental dysplasia of the hip,DDH)的关系。方法回顾性分析2017年1月至2022年12月在徐州医科大学附属徐州儿童医院进行DDH筛查的308名婴儿。根据筛查原因将其分为观察组(单纯皮纹不对称,n=116例)、临床异常组(具有髋关节弹响、髋关节外展受限、双下肢不等长等临床表现,n=92)和对照组(单纯行髋关节筛查,n=100)。分析临床评估结果及Graf法髋关节超声检查结果,比较各组病理性DDH的检出率,计算髋关节周围皮纹不对称诊断病理性DDH的阳性预测值、阴性预测值、敏感度、特异性。结果观察组检出病理性DDH 5例(4.31%);临床异常组检出病理性DDH 28例(30.43%);对照组检出病理性DDH 4例(4.00%)。观察组和对照组婴儿的病理性DDH检出率比较差异无统计学意义(χ^(2)=0.909,P=0.592);临床异常组婴儿的病理性DDH检出率显著高于对照组(χ^(2)=24.109,P<0.001)。单纯髋关节周围皮纹不对称对病理性DDH的阳性预测值为4.31%,阴性预测值为96.00%,敏感度为55.56%,特异性为46.38%。出现异常临床表现对病理性DDH的阳性预测值为30.43%,阴性预测值为96.00%,敏感度为87.50%,特异性为60.00%。结论单纯髋关节周围皮纹不对称诊断病理性DDH的价值较低,应更加重视是否存在其他临床体征,以指导婴儿的影像学筛查。
基金This study was supported by a grant from Key Project of National Natural Science Foundation （No. 30730092）.
文摘Deep skin wounds heal by scar formation with a Joss of its original appearance, structure and function.However, when the same damage occurs to the skin of an early gestational fetus, complete regeneration can be observed. Despite significant research in the field of skin regeneration, many mysteries remain, such as the loss of wound healing ability with maturity, the differences in healing at different parts of the body, and the presence of hypertrophic scars and keloids in some races but not in others. The finding of HOX genes in the skin provides new explanations to these conundrums.
