Fluorescently encoded microbeads are in demand for multiplexed applications in different fields.Compared to organic dye-based commercially available Luminex's x MAP technology, upconversion nanoparticles(UCNPs) ar...Fluorescently encoded microbeads are in demand for multiplexed applications in different fields.Compared to organic dye-based commercially available Luminex's x MAP technology, upconversion nanoparticles(UCNPs) are better alternatives due to their large antiStokes shift, photostability, nil background, and single wavelength excitation. Here, we developed a new multiplexed detection system using UCNPs for encoding poly(ethylene glycol) diacrylate(PEGDA) microbeads as well as for labeling reporter antibody. However, to prepare UCNPs-encoded microbeads, currently used swellingbased encapsulation leads to non-uniformity, which is undesirable for fluorescence-based multiplexing. Hence,we utilized droplet microfluidics to obtain encoded microbeads of uniform size, shape, and UCNPs distribution inside. Additionally, PEGDA microbeads lack functionality for probe antibodies conjugation on their surface.Methods to functionalize the surface of PEGDA microbeads(acrylic acid incorporation, polydopamine coating)reported thus far quench the fluorescence of UCNPs. Here,PEGDA microbeads surface was coated with silica followed by carboxyl modification without compromising the fluorescence intensity of UCNPs. In this study, droplet microfluidics-assisted UCNPs-encoded microbeads of uniform shape, size, and fluorescence were prepared.Multiple color codes were generated by mixing UCNPs emitting red and green colors at different ratios prior to encapsulation. UCNPs emitting blue color were used to label the reporter antibody. Probe antibodies were covalently immobilized on red UCNPs-encoded microbeads for specific capture of human serum albumin(HSA) as a model protein. The system was also demonstrated for multiplexed detection of both human C-reactive protein(hCRP) and HSA protein by immobilizing anti-h CRP antibodies on green UCNPs.展开更多
New initiatives put forward by clinical diagnosis require the development of technologies for high throughput screening(HTS) of multiple analytes. Suspension arrays have great advantages over the planar arraybased mul...New initiatives put forward by clinical diagnosis require the development of technologies for high throughput screening(HTS) of multiple analytes. Suspension arrays have great advantages over the planar arraybased multiplexing assays, and the encoded bead is the key for providing multiplexing capability. Among various encoding strategies, optically encoded microspheres have been widely used while the number of codes is still limited. This review discusses the progress of optical encoding strategy from mainly three aspects, namely organic dyes, quantum dots(QDs) and surface-enhanced Raman scattering(SERS) active substrates. Emphases are put up on describing how these optical encoded microbeads are manufactured and the merits and demerits of different encoding materials are compared.展开更多
Single-shot multi-frame phase imaging plays an important role in detecting continuous extreme physical phenomena,particularly suitable for measuring the density of media with non-repeatable changes and uncertainties.H...Single-shot multi-frame phase imaging plays an important role in detecting continuous extreme physical phenomena,particularly suitable for measuring the density of media with non-repeatable changes and uncertainties.However,traditional single-pattern multiplexed imaging faces challenges in retrieving amplitude and phase information of multiple frames without sacrificing spatial resolution and phase accuracy。展开更多
Single-shot ultrafast multidimensional optical imaging(UMOI)combines ultrahigh temporal resolution with multidimensional imaging capabilities in a snapshot,making it an essential tool for real-time detection and analy...Single-shot ultrafast multidimensional optical imaging(UMOI)combines ultrahigh temporal resolution with multidimensional imaging capabilities in a snapshot,making it an essential tool for real-time detection and analysis of ultrafast scenes.However,current single-shot UMOI techniques cannot simultaneously capture the spatial-temporal-spectral complex amplitude information,hampering it from complete analyses of ultrafast scenes.To address this issue,we propose a single-shot spatial-temporal-spectral complex amplitude imaging(STS-CAI)technique using wavelength and time multiplexing.By employing precise modulation of a broadband pulse via an encoding plate in coherent diffraction imaging and spatial-temporal shearing through a wide-open-slit streak camera,dual-mode multiplexing image reconstruction of wavelength and time is achieved,which significantly enhances the efficiency of information acquisition.Experimentally,a custom-built STS-CAI apparatus precisely measures the spatiotemporal characteristics of picosecond spatiotemporally chirped and spatial vortex pulses,respectively.STS-CAI demonstrates both ultrahigh temporal resolution and robust phase sensitivity.Prospectively,this technique is valuable for spatiotemporal coupling measurements of large-aperture ultrashort pulses and offers promising applications in both fundamental research and applied sciences.展开更多
The rapid developments of genomics and proteomics have driven the demand for multiplex and high throughput analysis of large numbers of biomolecules in the fields of medical diagnostics, drug discovery, and environmen...The rapid developments of genomics and proteomics have driven the demand for multiplex and high throughput analysis of large numbers of biomolecules in the fields of medical diagnostics, drug discovery, and environmental monitoring. Encoding the biomolecular binding events is the key technique to fulfill this demand, in which microparticles play the most important roles. This review outlines the development of multiplex and high throughput biodetections, and highlights the most recent advances in the field of encoding microparticles, together with problems that need to be resolved.展开更多
The quantum key distribution(QKD)network is a promising solution for secure communications.In this paper,we proposed a polarization-independent phase-modulated polarization encoding module,and it can be combined with ...The quantum key distribution(QKD)network is a promising solution for secure communications.In this paper,we proposed a polarization-independent phase-modulated polarization encoding module,and it can be combined with a dense wavelength division multiplexer(DWDM)to achieve multi-user QKD.We experimentally test the encoding module with a repetition rate of 62.5 MHz,and its average quantum bit error rate(QBER)is as low as 0.4%.Finally,we implement a principle verification test for simultaneous QKD for 1 to 2 users in 100 min,and the average QBER of two users under the transmission distance of 1 km and 5 km is kept below 0.8%.Due to the use of polarization encoding,the module can also realize scalable network architecture in free-space QKD systems in the future.展开更多
An algorithm is proposed for the fast reconstruction of off-axis digital holograms based on a combination of complex encoding（CE） and spatial multiplexing（SM）. In this algorithm, every two off-axis holograms recor...An algorithm is proposed for the fast reconstruction of off-axis digital holograms based on a combination of complex encoding（CE） and spatial multiplexing（SM）. In this algorithm, every two off-axis holograms recorded in sequence are first assembled into a CE hologram using the CE method, and then four of the CE holograms are again encoded into one complex spatial multiplexing（CSM） hologram based on the SM algorithm. It is demonstrated that the eight holograms encoded into such one CSM hologram can be quickly reconstructed by performing a two-dimensional（2D） Fourier transform（FT） on the CSM hologram. Using this method, the eight 2D FTs required for the reconstruction of the eight holograms in the conventional spatial filtering methods can be simplified to a process with only one 2D FT, which can largely improve the computation efficiency with the展开更多
基金the funding support from the Singapore Ministry of Education Academic Research Fund (AcRF Tier 3 Grant MOE2016-T3-1-004, R-397-000274-112 AcRF Tier 1 Grant R-397-000-270-114)
文摘Fluorescently encoded microbeads are in demand for multiplexed applications in different fields.Compared to organic dye-based commercially available Luminex's x MAP technology, upconversion nanoparticles(UCNPs) are better alternatives due to their large antiStokes shift, photostability, nil background, and single wavelength excitation. Here, we developed a new multiplexed detection system using UCNPs for encoding poly(ethylene glycol) diacrylate(PEGDA) microbeads as well as for labeling reporter antibody. However, to prepare UCNPs-encoded microbeads, currently used swellingbased encapsulation leads to non-uniformity, which is undesirable for fluorescence-based multiplexing. Hence,we utilized droplet microfluidics to obtain encoded microbeads of uniform size, shape, and UCNPs distribution inside. Additionally, PEGDA microbeads lack functionality for probe antibodies conjugation on their surface.Methods to functionalize the surface of PEGDA microbeads(acrylic acid incorporation, polydopamine coating)reported thus far quench the fluorescence of UCNPs. Here,PEGDA microbeads surface was coated with silica followed by carboxyl modification without compromising the fluorescence intensity of UCNPs. In this study, droplet microfluidics-assisted UCNPs-encoded microbeads of uniform shape, size, and fluorescence were prepared.Multiple color codes were generated by mixing UCNPs emitting red and green colors at different ratios prior to encapsulation. UCNPs emitting blue color were used to label the reporter antibody. Probe antibodies were covalently immobilized on red UCNPs-encoded microbeads for specific capture of human serum albumin(HSA) as a model protein. The system was also demonstrated for multiplexed detection of both human C-reactive protein(hCRP) and HSA protein by immobilizing anti-h CRP antibodies on green UCNPs.
基金the 2012 Shanghai Jiao Tong University and University of Michigan Collaborative Research Projects(No.12X120010007)the National High Technology Research and Development Program(863) of China(No.2012AA020103)+1 种基金the Shanghai Nano Program(No.11nm0505600)the Shanghai Jiao Tong University Funding(No.YG2012ZD03)
文摘New initiatives put forward by clinical diagnosis require the development of technologies for high throughput screening(HTS) of multiple analytes. Suspension arrays have great advantages over the planar arraybased multiplexing assays, and the encoded bead is the key for providing multiplexing capability. Among various encoding strategies, optically encoded microspheres have been widely used while the number of codes is still limited. This review discusses the progress of optical encoding strategy from mainly three aspects, namely organic dyes, quantum dots(QDs) and surface-enhanced Raman scattering(SERS) active substrates. Emphases are put up on describing how these optical encoded microbeads are manufactured and the merits and demerits of different encoding materials are compared.
基金China Postdoctoral Science Foundation(2023M743252,2024T170846)Key Research and Development Program of Zhejiang Province(2024SSYS0014)National Natural Science Foundation of China(62205304).
文摘Single-shot multi-frame phase imaging plays an important role in detecting continuous extreme physical phenomena,particularly suitable for measuring the density of media with non-repeatable changes and uncertainties.However,traditional single-pattern multiplexed imaging faces challenges in retrieving amplitude and phase information of multiple frames without sacrificing spatial resolution and phase accuracy。
基金supported by the National Natural Science Foundation of China(Grant Nos.12074121,12274139,and 12325408)the China Postdoctoral Science Foundation(Grant Nos.2023M743252 and 2024T170846)+1 种基金the Fundamental Research Funds for the Central Universities(Grant No.YZY24014)the Key Research and Development Program of Zhejiang Province(Grant No.2024SSYS0014).
文摘Single-shot ultrafast multidimensional optical imaging(UMOI)combines ultrahigh temporal resolution with multidimensional imaging capabilities in a snapshot,making it an essential tool for real-time detection and analysis of ultrafast scenes.However,current single-shot UMOI techniques cannot simultaneously capture the spatial-temporal-spectral complex amplitude information,hampering it from complete analyses of ultrafast scenes.To address this issue,we propose a single-shot spatial-temporal-spectral complex amplitude imaging(STS-CAI)technique using wavelength and time multiplexing.By employing precise modulation of a broadband pulse via an encoding plate in coherent diffraction imaging and spatial-temporal shearing through a wide-open-slit streak camera,dual-mode multiplexing image reconstruction of wavelength and time is achieved,which significantly enhances the efficiency of information acquisition.Experimentally,a custom-built STS-CAI apparatus precisely measures the spatiotemporal characteristics of picosecond spatiotemporally chirped and spatial vortex pulses,respectively.STS-CAI demonstrates both ultrahigh temporal resolution and robust phase sensitivity.Prospectively,this technique is valuable for spatiotemporal coupling measurements of large-aperture ultrashort pulses and offers promising applications in both fundamental research and applied sciences.
基金support of Grant for Teaching and Research of Excellent Young Teachers in Southeast University333 Talent Project Foundation and the Qing Lan Project of Jiangsu Province+2 种基金Jiangsu Science and Technology Department (BE2009148 & Bk2008318)the National Natural Science Foundation of China (50925309, 20703010 & 21073033)the Scientific Research Foundation of the Graduate School of Southeast University
文摘The rapid developments of genomics and proteomics have driven the demand for multiplex and high throughput analysis of large numbers of biomolecules in the fields of medical diagnostics, drug discovery, and environmental monitoring. Encoding the biomolecular binding events is the key technique to fulfill this demand, in which microparticles play the most important roles. This review outlines the development of multiplex and high throughput biodetections, and highlights the most recent advances in the field of encoding microparticles, together with problems that need to be resolved.
基金supported by the National Natural Science Foundation of China(Nos.61771205 and 62071186)Natural Science Foundation of Guangdong Province(No.2015A030313388)+1 种基金Science and Technology Planning Project of Guangdong Province(Nos.2015B010128012 and 2017KZ010101)Key Laboratory Foundation of Guangdong Province,China(No.2020B1212060066)。
文摘The quantum key distribution(QKD)network is a promising solution for secure communications.In this paper,we proposed a polarization-independent phase-modulated polarization encoding module,and it can be combined with a dense wavelength division multiplexer(DWDM)to achieve multi-user QKD.We experimentally test the encoding module with a repetition rate of 62.5 MHz,and its average quantum bit error rate(QBER)is as low as 0.4%.Finally,we implement a principle verification test for simultaneous QKD for 1 to 2 users in 100 min,and the average QBER of two users under the transmission distance of 1 km and 5 km is kept below 0.8%.Due to the use of polarization encoding,the module can also realize scalable network architecture in free-space QKD systems in the future.
基金supported by National Natural Science Foundation of China under Grant No.11474186
文摘An algorithm is proposed for the fast reconstruction of off-axis digital holograms based on a combination of complex encoding（CE） and spatial multiplexing（SM）. In this algorithm, every two off-axis holograms recorded in sequence are first assembled into a CE hologram using the CE method, and then four of the CE holograms are again encoded into one complex spatial multiplexing（CSM） hologram based on the SM algorithm. It is demonstrated that the eight holograms encoded into such one CSM hologram can be quickly reconstructed by performing a two-dimensional（2D） Fourier transform（FT） on the CSM hologram. Using this method, the eight 2D FTs required for the reconstruction of the eight holograms in the conventional spatial filtering methods can be simplified to a process with only one 2D FT, which can largely improve the computation efficiency with the
