[Objectives]To optimize the optimal extraction process of Qingdu Jianpi Mixture.[Methods]Taking water addition ratio,extraction time and extraction times as process investigation factors,psoralen content,astilbin cont...[Objectives]To optimize the optimal extraction process of Qingdu Jianpi Mixture.[Methods]Taking water addition ratio,extraction time and extraction times as process investigation factors,psoralen content,astilbin content and dry extract yield as evaluation indicators,the main influencing factors and level range of the extraction process of Qingdu Jianpi Mixture were determined on the basis of single factor test method,and the optimal weight coefficient was screened by AHP-entropy method mixed with weighting method.Combined with L_(9)(3^(4))orthogonal experiment,the best extraction process was obtained.At the same time,thin-layer chromatographic identification was used to identify Ficus simplicissima Lour.and Smilax glabra Roxb.in the medicinal liquid.[Results]The best extraction process:add 1:12 water to the prescription decoction pieces,extract under reflux for 2 times,1.5 h per time,and combine the filtrate to 250 mL.Thin layer chromatography analysis showed that the spots of Ficus simplicissima Lour.and Smilax glabra Roxb.in the medicinal solution were the same as those of reference substances at the corresponding positions,and the negative control had no interference.[Conclusions]The experimental method is reasonable and feasible,and the process is reliable,which can provide experimental reference for the subsequent application of in-hospital preparations and research and development of Qingdu Jianpi Mixture.展开更多
In order to comprehensively utilize the remaining bamboo residue of bamboo products,this paper presents a research on recycling the bamboo fibers from bamboo residue for improving the performance of the asphalt mixtur...In order to comprehensively utilize the remaining bamboo residue of bamboo products,this paper presents a research on recycling the bamboo fibers from bamboo residue for improving the performance of the asphalt mixtures.First of all,the basic performance parameters of sinocalamus affinis fiber,phyllostachys pubescens fiber,green bamboo fiber were tested and analyzed,and the optimal content and length were put forward.Then,the mix ratio design of the bamboo fiber modified asphalt mixture was further designed through the response surface method,and was verified the rationality of the mix ratio.Finally,the mixture specimens were made according to the experimental design mix ratio,and the high temperature,low temperature performance and moisture susceptibility of the bamboo fiber modified mixtures asphalt were tested.The results showed that the high temperature performance,low temperature performance and moisture susceptibility of bamboo fiber modified asphalt mixtures were improved compared with the performance of SBS modified asphalt mixture.When the length of bamboo fiber is 7.25 mm and the content of 0.22%,the road performance of the asphalt mixture was optimal.Consequentially,the decomposition of bamboo residue into bamboo fiber and its application in asphalt pavement can improve the reuse of bamboo waste,with remarkable environmental benefits and great promotion value.展开更多
New HPLC method was developed for determination of amlodipine and valsartan in their binary mixture as a part of routine control of combined formulations. The method was validated to meet official requirements includi...New HPLC method was developed for determination of amlodipine and valsartan in their binary mixture as a part of routine control of combined formulations. The method was validated to meet official requirements including selectivity, stability, linearity, precision and accuracy. Chromatography was carried out using a LiChrospher RP-18 column, a mixture containing acetonitrile, phosphate buffer of pH 3.5 and methanol (45:45:10, v/v/v) and new fluorescence detection at 255 nm for excitation and 448 nm for emission. The effect of methanol content, pH of the buffer, flow rate, detection wavelengths and column temperature was estimated in robustness study, according to a plan defined by the Plackett-Burman design. For identification of significant effects, both graphical and statistical methods were used. Ro-bustness for dissolution test was checked estimating the effects of paddle speed, temperature and pH of dissolution medium. The method was proved to complying with all official guidelines. Therefore, it is suitable for determination of amlodipine and valsartan in their binary mixtures for different analytical and pharmaceutical purposes.展开更多
基金Supported by Huang Ruisong's National Famous Old Traditional Chinese Medicine Expert Inheritance Studio Construction Project[GuoZhongYiYaoRenJiaoHan(2022)75]Hospital Pharmacy Research Project of Guangxi Pharmaceutical Association(GXYXH-202404)+4 种基金2024 Youth Science Fund Project of International Zhuang Medical Hospital(2024GZYJKT005)High-level Traditional Chinese Medicine Key Discipline Construction Project of National Administration of Traditional Chinese Medicine(ZYYZDXK-2023165)National Old Pharmaceutical Workers Inheritance Studio Construction Project of National Administration of Traditional Chinese Medicine[GuoZhongYiYaoRenJiaoHan(2024)255]Talent Cultivation Project-"Young Crop Project"of International Zhuang Medical Hospital Affiliated to Guangxi University of Chinese Medicine(2022001)Guangxi Traditional Chinese Medicine Multidisciplinary Innovation Team Project(GZKJ2309).
文摘[Objectives]To optimize the optimal extraction process of Qingdu Jianpi Mixture.[Methods]Taking water addition ratio,extraction time and extraction times as process investigation factors,psoralen content,astilbin content and dry extract yield as evaluation indicators,the main influencing factors and level range of the extraction process of Qingdu Jianpi Mixture were determined on the basis of single factor test method,and the optimal weight coefficient was screened by AHP-entropy method mixed with weighting method.Combined with L_(9)(3^(4))orthogonal experiment,the best extraction process was obtained.At the same time,thin-layer chromatographic identification was used to identify Ficus simplicissima Lour.and Smilax glabra Roxb.in the medicinal liquid.[Results]The best extraction process:add 1:12 water to the prescription decoction pieces,extract under reflux for 2 times,1.5 h per time,and combine the filtrate to 250 mL.Thin layer chromatography analysis showed that the spots of Ficus simplicissima Lour.and Smilax glabra Roxb.in the medicinal solution were the same as those of reference substances at the corresponding positions,and the negative control had no interference.[Conclusions]The experimental method is reasonable and feasible,and the process is reliable,which can provide experimental reference for the subsequent application of in-hospital preparations and research and development of Qingdu Jianpi Mixture.
基金Funded by the Key Research and Development Projects in Shaanxi Province(No.2022SF-328)Science and Technology Project of Shaanxi Department of Transportation(Nos.19-10K,19-28K)Science and Technology Project of Henan Department of Transportation(No.2020J-2-3)。
文摘In order to comprehensively utilize the remaining bamboo residue of bamboo products,this paper presents a research on recycling the bamboo fibers from bamboo residue for improving the performance of the asphalt mixtures.First of all,the basic performance parameters of sinocalamus affinis fiber,phyllostachys pubescens fiber,green bamboo fiber were tested and analyzed,and the optimal content and length were put forward.Then,the mix ratio design of the bamboo fiber modified asphalt mixture was further designed through the response surface method,and was verified the rationality of the mix ratio.Finally,the mixture specimens were made according to the experimental design mix ratio,and the high temperature,low temperature performance and moisture susceptibility of the bamboo fiber modified mixtures asphalt were tested.The results showed that the high temperature performance,low temperature performance and moisture susceptibility of bamboo fiber modified asphalt mixtures were improved compared with the performance of SBS modified asphalt mixture.When the length of bamboo fiber is 7.25 mm and the content of 0.22%,the road performance of the asphalt mixture was optimal.Consequentially,the decomposition of bamboo residue into bamboo fiber and its application in asphalt pavement can improve the reuse of bamboo waste,with remarkable environmental benefits and great promotion value.
文摘New HPLC method was developed for determination of amlodipine and valsartan in their binary mixture as a part of routine control of combined formulations. The method was validated to meet official requirements including selectivity, stability, linearity, precision and accuracy. Chromatography was carried out using a LiChrospher RP-18 column, a mixture containing acetonitrile, phosphate buffer of pH 3.5 and methanol (45:45:10, v/v/v) and new fluorescence detection at 255 nm for excitation and 448 nm for emission. The effect of methanol content, pH of the buffer, flow rate, detection wavelengths and column temperature was estimated in robustness study, according to a plan defined by the Plackett-Burman design. For identification of significant effects, both graphical and statistical methods were used. Ro-bustness for dissolution test was checked estimating the effects of paddle speed, temperature and pH of dissolution medium. The method was proved to complying with all official guidelines. Therefore, it is suitable for determination of amlodipine and valsartan in their binary mixtures for different analytical and pharmaceutical purposes.
