摘要
背景长白山区作为东北重要生态功能区,大型真菌资源丰富,其中荷叶离褶伞因独特食药用价值备受关注。目的促进该野生菌株资源的保护与可持续利用,建立高效培养体系。方法通过多阶段优化策略优化长白山区野生荷叶离褶伞菌株培养条件,采用单因素试验结合Plackett-Burman试验筛选关键影响因素,运用响应面法建立预测模型优化液体培养基配方。此外,筛选二级种配方并采用混料均匀试验设计优化固体培养基配方。结果确定液体培养显著因子为葡萄糖、玉米粉和硫酸镁,响应面法建立预测模型(R2=0.9841)优化获得的最佳培养条件为葡萄糖26.135 g/L、玉米粉31.074 g/L、硫酸镁1.517 g/L,在此条件下菌丝生物量达(21.89±0.20)g/L。二级种麦粒培养基中添加木屑菌丝生长速度达到(2.62±0.38)mm/d,瓶栽三级种配方基质最佳配比为24%玉米芯、43%木屑、33%棉籽壳,该配方中菌丝生长速度为(1.51±0.03)mm/d,头潮菇产量为(44.33±3.05)g,头潮菇生物学效率为(44.00±3.46)%,建立的数学模型(R2>0.9)可准确预测各因素交互作用对菌丝生长速度和子实体产量的影响。结论长白山区荷叶离褶伞菌株对营养因子适应性广,建立的响应面模型可准确预测该菌株在不同状态培养条件中的生长情况。
Background As an important ecological functional area in northeast China,the Changbai Mountain region is rich in macrofungal resources,among which Lyophyllum decastes has garnered significant attention owing to its unique edible and medicinal values.Objective To facilitate the protection and sustainable utilization of the wild strain resources of this species and establish an efficient culture system.Methods We employed a multi-stage optimization strategy to optimize the culture conditions for the wild L.decastes strain isolated from the Changbai Mountain region.The key influencing factors were screened by single factor tests combined with the Plackett-Burman test,and the response surface method was utilized to establish a prediction model to optimize the liquid medium formulas.Additionally,the medium formulas for secondary culture were screened,and the mixture uniform design was adopted to optimize the solid medium formula for tertiary culture.Results The significant factors affecting the liquid culture were glucose,corn flour,and magnesium sulfate.A prediction model was established via the response surface method(R2=0.9841).The optimal culture conditions were as follows:glucose 26.135 g/L,corn flour 31.074 g/L,and magnesium sulfate 1.517 g/L.Under these conditions,the mycelial biomass was(21.89±0.20)g/L.The mycelial growth rate in the wheat grain medium supplemented with sawdust for secondary culture reached(2.62±0.38)mm/d.The optimal medium formula for the tertiary culture was composed of 24%corncob,43%sawdust,and 33%cotton seed hull.The mycelial growth rate in the medium prepared with this formula was(1.51±0.03)mm/d.The yield of the first flush of mushrooms was(44.33±3.05)g,and the biological efficiency of the first flush was(44.00±3.46)%.The established mathematical model(R2>0.9)can accurately predict the effects of interactions among various factors on the mycelial growth rate and fruiting body yield.Conclusion The L.decastes strain exhibits broad adaptability to nutrient factors,and the established model can accurately predict the growth of this strain under different culture conditions.
作者
孔雨非
张雪超
李晓滨
梁运江
徐济责
KONG Yufei;ZHANG Xuechao;LI Xiaobin;LIANG Yunjiang;XU Jize(Agriculture College,Yanbian University,Yanji 133000,Jilin,China;Agriculture College,Jilin Agricultural Science and Technology University,Jilin 132000,Jilin,China;Jilin Province Gardening Specialty Management Station,Changchun 130000,Jilin,China)
出处
《微生物学通报》
北大核心
2026年第2期989-1006,共18页
Microbiology China
基金
吉林省现代农业产业技术体系食用菌产业技术体系(JLARS-2025-060205)。
关键词
荷叶离褶伞
液、固体培养基配方优化
响应面法
混料设计
模型
Lyophyllum decastes
optimization of liquid and solid medium formulas
response surface method
mixture design
model
