Objective To evaluate whether the thermotaxis tracking model is suitable for assessing long-term memory （LTM） in the nematode Caenorhabditis elegans. Methods Animals were trained at 20℃ overnight in presence of foo...Objective To evaluate whether the thermotaxis tracking model is suitable for assessing long-term memory （LTM） in the nematode Caenorhabditis elegans. Methods Animals were trained at 20℃ overnight in presence of food. The percentage of animals performing isothermal tracking （IT） behavior was measured at different time intervals after the training. Results The percentage of animals performing IT behavior, the numbers of body bends inside and outside the training temperature, and the expression patterns of AFD and AIY neurons were similar to those in control animals at 36 and 48 h after training; whereas when extending to 60, 72, and 84 h, locomotory behavior defects were observed in the assayed animals, suggesting that this thermal tracking model is feasible for analyzing LTM at 36 and 48 h after training. Moreover, the percent-age of animals performing IT behavior was reduced at 18, 36, and 48 h after training in neuronal calcium sensor-1 gene （nsc-1） mutant animals compared with that in wild-type N2 animals. In addition, exposure to plumbum （Pb） significantly repressed the LTM at 18, 36, and 48 h after training in both wild-type N2 and ncs-1 mutant animals. Conclusion The thermotaxis tracking model is suitable for evaluating the LTM regulated by NCS-1, and can be employed for elucidating regulatory functions of specific genes or effects of stimuli on memory in C. elegans.展开更多
AIM To determine vitamin B 1 in pharmaceutical preparations by chemiluminescence (CL) flow sensor. METHODS When 200 μL of Na 3PO 4 was passed through an anion exchange column, K 3Fe(CN) 6 was eluted from the resin an...AIM To determine vitamin B 1 in pharmaceutical preparations by chemiluminescence (CL) flow sensor. METHODS When 200 μL of Na 3PO 4 was passed through an anion exchange column, K 3Fe(CN) 6 was eluted from the resin and then mixed with the vitamin B 1 stream containing NaOH to product CL, by the fast oxidation reaction between vitamin B 1 and K 3Fe(CN) 6 in alkaline solution. RESULTS The CL emission intensity was correlated with the vitamin B 1 concentration in the range 1 0×10 -5 to 1 0×10 -3 mol·L -1 and the detection limit was 8 0×10 -6 mol·L -1 (3σ). A complete analysis, including sampling and washing, can be performed within 2 min with a relative standard deviation of less than 4 0%. CONCLUSION The vitamin B 1 flow sensor was stable for over 200 analyses and has been applied successfully to determination of vitamin B 1 in pharmaceutical preparations.展开更多
文摘Objective To evaluate whether the thermotaxis tracking model is suitable for assessing long-term memory （LTM） in the nematode Caenorhabditis elegans. Methods Animals were trained at 20℃ overnight in presence of food. The percentage of animals performing isothermal tracking （IT） behavior was measured at different time intervals after the training. Results The percentage of animals performing IT behavior, the numbers of body bends inside and outside the training temperature, and the expression patterns of AFD and AIY neurons were similar to those in control animals at 36 and 48 h after training; whereas when extending to 60, 72, and 84 h, locomotory behavior defects were observed in the assayed animals, suggesting that this thermal tracking model is feasible for analyzing LTM at 36 and 48 h after training. Moreover, the percent-age of animals performing IT behavior was reduced at 18, 36, and 48 h after training in neuronal calcium sensor-1 gene （nsc-1） mutant animals compared with that in wild-type N2 animals. In addition, exposure to plumbum （Pb） significantly repressed the LTM at 18, 36, and 48 h after training in both wild-type N2 and ncs-1 mutant animals. Conclusion The thermotaxis tracking model is suitable for evaluating the LTM regulated by NCS-1, and can be employed for elucidating regulatory functions of specific genes or effects of stimuli on memory in C. elegans.
文摘AIM To determine vitamin B 1 in pharmaceutical preparations by chemiluminescence (CL) flow sensor. METHODS When 200 μL of Na 3PO 4 was passed through an anion exchange column, K 3Fe(CN) 6 was eluted from the resin and then mixed with the vitamin B 1 stream containing NaOH to product CL, by the fast oxidation reaction between vitamin B 1 and K 3Fe(CN) 6 in alkaline solution. RESULTS The CL emission intensity was correlated with the vitamin B 1 concentration in the range 1 0×10 -5 to 1 0×10 -3 mol·L -1 and the detection limit was 8 0×10 -6 mol·L -1 (3σ). A complete analysis, including sampling and washing, can be performed within 2 min with a relative standard deviation of less than 4 0%. CONCLUSION The vitamin B 1 flow sensor was stable for over 200 analyses and has been applied successfully to determination of vitamin B 1 in pharmaceutical preparations.
