The in vitro and in vivo effects of corn peptides（CPs） prepared from corn gluten meal by proteolysis with an alkaline protease and fractions of CPs from Sephadex G-15 and G-10 columns on activities of alcohol dehydr...The in vitro and in vivo effects of corn peptides（CPs） prepared from corn gluten meal by proteolysis with an alkaline protease and fractions of CPs from Sephadex G-15 and G-10 columns on activities of alcohol dehydroge-nase（ADH） were studied. The results show that CPs and fraction 3 of CPs from Sephadex G-10 column enhance in vitro ADH activity. Furthermore, the in vitro accelerating effect of the fraction 3 of CPs on ADH activity was superior to that of glutathione, which was also found even in the presence of ADH inhibitor, such as pyrazole. In the in vivo experiments, the animals were fed with different dosages of CPs and with a dose of Chinese distilled spirit orally, and sacrificed for the measurement of ADH activity. In vivo experimental results indicate that CPS enhanced hepatic ADH activities. To test the safety of CPs as health food, 30 d feeding test was performed. No obvious toxic effects were detected in treated Wistar rats.展开更多
This study aims at the effects of an intake of low molecular weight corn peptides(LMCPs) prepared from zein on alcohol metablism in rats. LMCPs(1.0 g/kg body weight) in 15% ethanol(10 mL/kg body weight) were given to ...This study aims at the effects of an intake of low molecular weight corn peptides(LMCPs) prepared from zein on alcohol metablism in rats. LMCPs(1.0 g/kg body weight) in 15% ethanol(10 mL/kg body weight) were given to Wister rats by intragastric gavage. The assay of blood ethanol was conducted by using the enzyme based assay kit. The amino acid analysis was made with an amino acid analyzer. The data of the animal experiments showed that LMCPs could accelerate the metabolism of alcohol in rats. In the control group, the blood ethanol concentration reached the maximum level of (827.0±77.3) mg/L after ethanol loading for 30 min, then gradually decreased. In contrast, the blood ethanol concentration only reached (527.25±47.0) mg/L after 30 min in the group of LMCPs taken. These results indicate that LMCPs could decrease ethanol concentration in blood rapidly.展开更多
Corn peptides(CP) were prepared from zein via proteolysis by means of a type of alkaline protease, Alcalase, at 50 ℃ and pH 8.0. The anti oxidative activity of the hydrolysate was examined. It was found that the...Corn peptides(CP) were prepared from zein via proteolysis by means of a type of alkaline protease, Alcalase, at 50 ℃ and pH 8.0. The anti oxidative activity of the hydrolysate was examined. It was found that the anti oxidative activity reached the maximum at the fourth hour of the hydrolysis and then decreased. The effect of the enzyme dosage on the solubility of corn protein, the dependence of hydrolysis time on the degree of the hydrolysis(DH) and molecular weight distribution were examined respectively. 30% DH was controlled on the basis of anti oxidative activity. The hydrolysate was fractionated on a Sephadex G 15 column. The anti oxidative activities were examined for all the fractions. The main fraction for anti oxidative activity was collected and characterized by the methods of amino acid analysis, the measurement of average chain length and etc .. The main M W distribution of the main fraction was 300-600 Daltons. The anti oxidative properties of the small peptides in vitro were studied. It was found that they could inhibit the auto oxidation of pyrogallol and the oxidation of hemoglobin. The hemolysis degree of erythrocyte caused by hydroxyl free radicals was decreased greatly, indicating a protective effect of the anti oxidative peptides on the cell membrane damage of erythrocyte.展开更多
The effects of the ingestion of corn peptides with a low molecular weight(LMCP) prepared from zein on some plasma free amino acid concentrations in rats that had taken ethanol were investigated. LMCP(1 0 g/kg bod...The effects of the ingestion of corn peptides with a low molecular weight(LMCP) prepared from zein on some plasma free amino acid concentrations in rats that had taken ethanol were investigated. LMCP(1 0 g/kg body weight) in 15% ethanol(10 mL/kg body weight) was given to Wister rats by intragastrical administration. The amino acid analysis showed that the concentrations of alanine, leucine, and proline in the plasma reached their maximum levels at 30 min for the LMCP-intake group. They are 582.39, 99.60 and 272.51 μg/L , respectively. But in the control group, the plasma free amino acid levels were not changed obviously. Therefore, LMCP could cause an increase in concentration of some free amino acids such as alanine, leucine and proline etc . in plasma of the rats that have taken ethanol.展开更多
[Objective] To investigate the optimal enzyme for the hydrolysis of corn gluten meal and the optimal hydrolysis conditions for the enzyme. [Method] Nine kinds of enzymes were used to hydrolyze the corn gluten meal, us...[Objective] To investigate the optimal enzyme for the hydrolysis of corn gluten meal and the optimal hydrolysis conditions for the enzyme. [Method] Nine kinds of enzymes were used to hydrolyze the corn gluten meal, using the formaldehyde titration method for the determination of hydrolysis degree, and orthogonal test was used to determine the optimal hydrolysis conditions for double enzymes hydrol- ysis of corn gluten meal. [Result] The optimal pretreatment condition for corn gluten meal is heating at 121 ~C for 30 min. The double enzyme hydrolysis for the pro- treated corn gluten meal using 2709 alkaline protease and flavourzyme showed that the degree of hydrolysis could reach 32.4% with enzyme addition amount of 4%, hy- drolysis time of 4 h at 45℃ and pH=7.0. [Conclusion] This study laid the foundation for the study on the preparation of bioactive peptides such as oligopeptide with high F value and antihypertensive peptides, further improving the corn intensive process- ing industrial chain.展开更多
为探究胃肠消化对玉米活性肽α-葡萄糖苷酶抑制活性及抗氧化活性的影响,分别对消化前后玉米活性肽未超滤和分子量<1000 Da 2个组分的粒径分布、氨基酸组成、α-葡萄糖苷酶抑制活性、DPPH、ABTS^(+)自由基清除率进行比较分析。结果表...为探究胃肠消化对玉米活性肽α-葡萄糖苷酶抑制活性及抗氧化活性的影响,分别对消化前后玉米活性肽未超滤和分子量<1000 Da 2个组分的粒径分布、氨基酸组成、α-葡萄糖苷酶抑制活性、DPPH、ABTS^(+)自由基清除率进行比较分析。结果表明:玉米活性肽经体外模拟胃肠消化后,2个组分的平均粒径分别降低52.01%和66.92%,氨基酸组成变化无统计学意义,未超滤组分的α-葡萄糖苷酶抑制活性和DPPH自由基清除率分别提高至48.38%±1.88%和43.21%±2.21%,ABTS^(+)自由基清除率变化无统计学意义;分子量<1000Da组分的α-葡萄糖苷酶抑制活性和ABTS^(+)自由基清除率变化无统计学意义,DPPH自由基清除率降低了1.97%。因此,玉米活性肽能够抵抗胃肠消化,且消化产物持有更佳的降血糖及抗氧化活性,为玉米活性肽在功能食品中的应用提供了理论基础。展开更多
目的基于代谢组学探究玉米肽通过调控粪便代谢产物及通路参与肥胖小鼠体重和血脂调节的可能机制。方法通过高脂饮食构建C57BL/6小鼠肥胖模型,分别采用玉米肽灌胃、玉米肽联合游泳运动、游泳运动、奥利司他对肥胖小鼠进行8周干预。干预...目的基于代谢组学探究玉米肽通过调控粪便代谢产物及通路参与肥胖小鼠体重和血脂调节的可能机制。方法通过高脂饮食构建C57BL/6小鼠肥胖模型,分别采用玉米肽灌胃、玉米肽联合游泳运动、游泳运动、奥利司他对肥胖小鼠进行8周干预。干预后检测小鼠体质量、血脂水平,采集小鼠粪便样品通过液相色谱-串联质谱技术(liquid chromatography-tandem mass spectrometry,LC-MS/MS)分析粪便代谢物,利用Analyst1.6.3、MultiaQuant处理质谱数据并进行多元统计及聚类分析,通过正交偏最小二乘判别(orthogonal partial least squares discriminant analysis,OPLS-DA)分析组间代谢物的总体差异,采用京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)数据库对差异代谢物进行通路富集分析。结果与肥胖对照组相比,玉米肽干预组小鼠体重虽未显著下降,但体重增长幅度相对缓慢,血清甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)显著降低(P<0.05,P<0.01);玉米肽联合游泳组小鼠体重极显著下降(P<0.01),总胆固醇(total cholesterol,TC)、TG、LDL-C均显著降低(P<0.05,P<0.01)。代谢组学结果表明肥胖小鼠粪便代谢轮廓与正常对照组相比发生显著变化。对各干预组与肥胖对照组间的差异代谢物进行交集筛选,共发现26个共有的差异代谢产物,其中,与模型组相比,干预组可以显著回调胞嘧啶、溶血磷脂酰乙醇胺(lysophosphatidylethanolamine,LPE)(0:0/16:1)、LPE(16:1/0:0)、LPE(17:1/0:0)这4种代谢物的水平,可以显著下调D-松三糖、棉籽糖、3,5-二碘-L-酪氨酸、7,8-二氢-L-生物蝶呤及亮氨酸-丙氨酸-缬氨酸这5种代谢物的水平,玉米肽、玉米肽辅以游泳运动干预分别能显著调节其中8个、9个差异代谢物。代谢通路分析共确定9条与肥胖发生显著相关的代谢通路,玉米肽及玉米肽辅以游泳运动干预共有的显著代谢通路有4条,即甘油磷酸脂代谢、嘧啶代谢、酪氨酸代谢及叶酸合成通路。结论玉米肽能够有效改善高脂膳食导致的粪便代谢产物失调,并调节与能量代谢相关的一些代谢产物及相关信号通路来实现其对体重和血脂的调节作用,且玉米肽辅以游泳运动干预效果更佳。展开更多
基金Supported by the Science Research Foundation of Jilin Agricultural University, China(No.205-00370)
文摘The in vitro and in vivo effects of corn peptides（CPs） prepared from corn gluten meal by proteolysis with an alkaline protease and fractions of CPs from Sephadex G-15 and G-10 columns on activities of alcohol dehydroge-nase（ADH） were studied. The results show that CPs and fraction 3 of CPs from Sephadex G-10 column enhance in vitro ADH activity. Furthermore, the in vitro accelerating effect of the fraction 3 of CPs on ADH activity was superior to that of glutathione, which was also found even in the presence of ADH inhibitor, such as pyrazole. In the in vivo experiments, the animals were fed with different dosages of CPs and with a dose of Chinese distilled spirit orally, and sacrificed for the measurement of ADH activity. In vivo experimental results indicate that CPS enhanced hepatic ADH activities. To test the safety of CPs as health food, 30 d feeding test was performed. No obvious toxic effects were detected in treated Wistar rats.
基金Supported by the Grant 94 35 4 6 - 3from Science and Technology Commission of Jilin Province
文摘This study aims at the effects of an intake of low molecular weight corn peptides(LMCPs) prepared from zein on alcohol metablism in rats. LMCPs(1.0 g/kg body weight) in 15% ethanol(10 mL/kg body weight) were given to Wister rats by intragastric gavage. The assay of blood ethanol was conducted by using the enzyme based assay kit. The amino acid analysis was made with an amino acid analyzer. The data of the animal experiments showed that LMCPs could accelerate the metabolism of alcohol in rats. In the control group, the blood ethanol concentration reached the maximum level of (827.0±77.3) mg/L after ethanol loading for 30 min, then gradually decreased. In contrast, the blood ethanol concentration only reached (527.25±47.0) mg/L after 30 min in the group of LMCPs taken. These results indicate that LMCPs could decrease ethanol concentration in blood rapidly.
基金Supported by the Science and Technology Comm ission of Jinlin Province(No. 94 35 4 6 - 3)
文摘Corn peptides(CP) were prepared from zein via proteolysis by means of a type of alkaline protease, Alcalase, at 50 ℃ and pH 8.0. The anti oxidative activity of the hydrolysate was examined. It was found that the anti oxidative activity reached the maximum at the fourth hour of the hydrolysis and then decreased. The effect of the enzyme dosage on the solubility of corn protein, the dependence of hydrolysis time on the degree of the hydrolysis(DH) and molecular weight distribution were examined respectively. 30% DH was controlled on the basis of anti oxidative activity. The hydrolysate was fractionated on a Sephadex G 15 column. The anti oxidative activities were examined for all the fractions. The main fraction for anti oxidative activity was collected and characterized by the methods of amino acid analysis, the measurement of average chain length and etc .. The main M W distribution of the main fraction was 300-600 Daltons. The anti oxidative properties of the small peptides in vitro were studied. It was found that they could inhibit the auto oxidation of pyrogallol and the oxidation of hemoglobin. The hemolysis degree of erythrocyte caused by hydroxyl free radicals was decreased greatly, indicating a protective effect of the anti oxidative peptides on the cell membrane damage of erythrocyte.
基金Supported by the Science and Technology Comm ission of Jilin Province( No.94 35 4 6 - 3)
文摘The effects of the ingestion of corn peptides with a low molecular weight(LMCP) prepared from zein on some plasma free amino acid concentrations in rats that had taken ethanol were investigated. LMCP(1 0 g/kg body weight) in 15% ethanol(10 mL/kg body weight) was given to Wister rats by intragastrical administration. The amino acid analysis showed that the concentrations of alanine, leucine, and proline in the plasma reached their maximum levels at 30 min for the LMCP-intake group. They are 582.39, 99.60 and 272.51 μg/L , respectively. But in the control group, the plasma free amino acid levels were not changed obviously. Therefore, LMCP could cause an increase in concentration of some free amino acids such as alanine, leucine and proline etc . in plasma of the rats that have taken ethanol.
文摘[Objective] To investigate the optimal enzyme for the hydrolysis of corn gluten meal and the optimal hydrolysis conditions for the enzyme. [Method] Nine kinds of enzymes were used to hydrolyze the corn gluten meal, using the formaldehyde titration method for the determination of hydrolysis degree, and orthogonal test was used to determine the optimal hydrolysis conditions for double enzymes hydrol- ysis of corn gluten meal. [Result] The optimal pretreatment condition for corn gluten meal is heating at 121 ~C for 30 min. The double enzyme hydrolysis for the pro- treated corn gluten meal using 2709 alkaline protease and flavourzyme showed that the degree of hydrolysis could reach 32.4% with enzyme addition amount of 4%, hy- drolysis time of 4 h at 45℃ and pH=7.0. [Conclusion] This study laid the foundation for the study on the preparation of bioactive peptides such as oligopeptide with high F value and antihypertensive peptides, further improving the corn intensive process- ing industrial chain.
文摘为探究胃肠消化对玉米活性肽α-葡萄糖苷酶抑制活性及抗氧化活性的影响,分别对消化前后玉米活性肽未超滤和分子量<1000 Da 2个组分的粒径分布、氨基酸组成、α-葡萄糖苷酶抑制活性、DPPH、ABTS^(+)自由基清除率进行比较分析。结果表明:玉米活性肽经体外模拟胃肠消化后,2个组分的平均粒径分别降低52.01%和66.92%,氨基酸组成变化无统计学意义,未超滤组分的α-葡萄糖苷酶抑制活性和DPPH自由基清除率分别提高至48.38%±1.88%和43.21%±2.21%,ABTS^(+)自由基清除率变化无统计学意义;分子量<1000Da组分的α-葡萄糖苷酶抑制活性和ABTS^(+)自由基清除率变化无统计学意义,DPPH自由基清除率降低了1.97%。因此,玉米活性肽能够抵抗胃肠消化,且消化产物持有更佳的降血糖及抗氧化活性,为玉米活性肽在功能食品中的应用提供了理论基础。
文摘目的基于代谢组学探究玉米肽通过调控粪便代谢产物及通路参与肥胖小鼠体重和血脂调节的可能机制。方法通过高脂饮食构建C57BL/6小鼠肥胖模型,分别采用玉米肽灌胃、玉米肽联合游泳运动、游泳运动、奥利司他对肥胖小鼠进行8周干预。干预后检测小鼠体质量、血脂水平,采集小鼠粪便样品通过液相色谱-串联质谱技术(liquid chromatography-tandem mass spectrometry,LC-MS/MS)分析粪便代谢物,利用Analyst1.6.3、MultiaQuant处理质谱数据并进行多元统计及聚类分析,通过正交偏最小二乘判别(orthogonal partial least squares discriminant analysis,OPLS-DA)分析组间代谢物的总体差异,采用京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)数据库对差异代谢物进行通路富集分析。结果与肥胖对照组相比,玉米肽干预组小鼠体重虽未显著下降,但体重增长幅度相对缓慢,血清甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)显著降低(P<0.05,P<0.01);玉米肽联合游泳组小鼠体重极显著下降(P<0.01),总胆固醇(total cholesterol,TC)、TG、LDL-C均显著降低(P<0.05,P<0.01)。代谢组学结果表明肥胖小鼠粪便代谢轮廓与正常对照组相比发生显著变化。对各干预组与肥胖对照组间的差异代谢物进行交集筛选,共发现26个共有的差异代谢产物,其中,与模型组相比,干预组可以显著回调胞嘧啶、溶血磷脂酰乙醇胺(lysophosphatidylethanolamine,LPE)(0:0/16:1)、LPE(16:1/0:0)、LPE(17:1/0:0)这4种代谢物的水平,可以显著下调D-松三糖、棉籽糖、3,5-二碘-L-酪氨酸、7,8-二氢-L-生物蝶呤及亮氨酸-丙氨酸-缬氨酸这5种代谢物的水平,玉米肽、玉米肽辅以游泳运动干预分别能显著调节其中8个、9个差异代谢物。代谢通路分析共确定9条与肥胖发生显著相关的代谢通路,玉米肽及玉米肽辅以游泳运动干预共有的显著代谢通路有4条,即甘油磷酸脂代谢、嘧啶代谢、酪氨酸代谢及叶酸合成通路。结论玉米肽能够有效改善高脂膳食导致的粪便代谢产物失调,并调节与能量代谢相关的一些代谢产物及相关信号通路来实现其对体重和血脂的调节作用,且玉米肽辅以游泳运动干预效果更佳。
